T. Mini Shobi | Bharathidhasan University (original) (raw)
Papers by T. Mini Shobi
Asian Journal of Pharmaceutical and Clinical Research, 2015
Objective: This study was subjected to analyse the technique for the determination of quercetin, ... more Objective: This study was subjected to analyse the technique for the determination of quercetin, a flavonoid in onion ( Allium cepa L.) from three different cultivars of bulb onion (O1, O2 and O3) which were extracted with petroleum ether, chloroform, methanol and water. Also this study was aimed to determine the antimicrobial activity of four solvent extracts of three onion cultivars against ten MTCC bacterial pathogens. Methods: Quercetin in onion was determined by analytical technique, HPTLC and the antimicrobial activity was performed by well diffusion method. Result: Quercetin was determined in all the three cultivars with Rf value 0.68. Quercetin was also extracted in all the four solvent extracts of onion cultivars. Apart from quercetin, few more compounds also isolated. More compounds were isolated in O3. In the determination of antimicrobial activity of onion extracts against MTCC cultures revealed that all the three onion cultivars were highly active against Staphylococc...
International Journal of Current Research in Biosciences and Plant Biology, 2017
An analytical technique for the determination of volatile phytochemical compounds by GC-MS in oni... more An analytical technique for the determination of volatile phytochemical compounds by GC-MS in onion, Allium cepa L., was reported here. Three different cultivars of onion bulb (O1, O2 and O3) were extracted with petroleum ether, and the volatile compounds were analyzed by GC-MS. Terpenes such as humulaneand phytosterols and aldehydes are found common in all the three cultivars. Thiophenes and disulphides were identified as major compounds in O1 and O3 respectively. Caryophyllene, a terpene was observed in both O1 and O2. Squalene, allicin and sulphide compounds were identified specifically in O3. The study further reported that the composition of volatile compounds in the cultivars is varied and these differences may be due to the variations in geographic, climatic, soil fertility and cultivation practices. The intensity of flavour is governed by hereditary factors and environmental conditions under which the onions grow.
GSC Biological and Pharmaceutical Sciences, 2018
A b s t r a c t A r t i c l e I n f o Begonia fallax A.DC. belongs to the family of Begoniaceae a... more A b s t r a c t A r t i c l e I n f o Begonia fallax A.DC. belongs to the family of Begoniaceae and is an endemic species to Peninsular India. Shoot tip and nodal explants were screened using Murashige and Skoog medium supplemented with BAP, KIN and TDZ individually and combination with IAA, NAA and IBA for multiple shoot initiation. BAP and NAA (0.5+0.5 mg-l) produced 20.8 shoots with up to 4.0 cm shoot length to nodal explants whereas 17.9 shoots with 3.1 cm shoot length to shoot tip explants. For root induction, various concentrations of IAA, NAA and IBA were used individually. The highest rooting frequency was achieved to nodal explants (64.90 roots) on MS medium supplemented with 0.5 mg-l NAA compared to shoot tip explants (31.90 roots). Invariably, root length was up to 2.3 cm to both the explants. The rooted explants were transplanted into plastic cups and showed 70% survival rate in the Environmental Growth Chamber. Nodal explants could be the best for large-scale multiplication and propagation to facilitate in vitro conservation and for secondary metabolites production.
Garlic, Allium sativum L. is a plant with so many medicinal properties and as therapeutic agent w... more Garlic, Allium sativum L. is a plant with so many medicinal properties and as therapeutic agent
with a peculiar odor. It was used as antiseptic to prevent gangrene during the two world wars and was
ingested as stimulant by the athletes during the first Olympic Games in Greece. An antiseptic lotion
prepared out of it is used to clean wounds and ulcers in India. Onion and garlic tea is suggested to treat
fever, headache, cholera and dysentery in China. Due to its medicinal property, it is used to treat many
disorders right from respiratory ailments, asthma, pneumonia, diabetes, cardiovascular disorders to
rheumatism. The medicinal property is due to the presence of a large number of bioactive compounds.
The unique odour is attributed for the presence of organosulfur compounds which can be analyzed by
GC-MS. Major bioactive components identified in the present study are allicin, diallyl disulfide, allyl
alcohol, alliin, diallylsulphide, dimethyl sulphate, dimethyl disulfide, diallyl ether, allyl methyl disulfide,
allyl propyl sulphide, dimethyl propyl disulfide, dipropyl disulfide, allyl methyl sulphide and
trichloroethylene.
Objective: This study was subjected to analyze the technique for the determination of quercetin, ... more Objective: This study was subjected to analyze the technique for the determination of quercetin, a flavonoid in onion (Allium cepa L.) from three different cultivars of bulb onion (O1, O2, and O3) which were extracted with petroleum ether, chloroform, methanol, and water. Furthermore, this study was aimed to determine the antimicrobial activity of four solvent extracts of three onion cultivars against 10 microbial type culture collection (MTCC) bacterial pathogens.
Methods: Quercetin in onion was determined by analytical technique, high-performance thin layer chromatography, and the antimicrobial activity were performed by the well diffusion method.
Result: Quercetin was determined in all the three cultivars with retention factor value 0.68. Quercetin was also extracted in all the four solvent extracts of onion cultivars. Apart from quercetin, few more compounds also isolated. More compounds were isolated in O3. In the determination of the antimicrobial activity of onion extracts against MTCC cultures revealed that all the three onion cultivars were highly active against Staphylococcus aureus, it showed more than 25 mm zone of inhibition. Among the three onion cultivars, O2 showed better antimicrobial activity against all the tested bacterial cultures.
Conclusion: This study showed that onion has good broad spectrum bioactivity against microorganisms revealed that it contains phytochemicals which can be used as nutraceuticals.
This study was subjected to analyse the technique for the determination of quercetin, a flavonoid... more This study was subjected to analyse the technique for the determination of quercetin, a flavonoid in garlic (Allium
sativum L.) from three different cultivars of bulb onion (G1, G2 and G3) which were extracted with petroleum ether,
chloroform, methanol and water. Also this study was aimed to determine the antimicrobial activity of four solvent
extracts of garlic cultivar against ten MTCC bacterial pathogens. Quercetin in garlic was determined by analytical
technique, HPTLC and the antimicrobial activity was performed by well diffusion method. Quercetin was
determined in all the three cultivars with Rf value 0.68. Quercetin was also extracted in all the four solvent extracts
of garlic cultivars. Apart from quercetin, few more compounds also isolated. More compounds were isolated in G1.
In the determination of antimicrobial activity of onion extracts against MTCC cultures revealed that onion cultivar
was highly active against Staphylococcus aureus, it showed more than 25mm zone of inhibition. This study showed
that onion has good broad spectrum bioactivity against microorganisms revealed that it contains phytochemicals
which can be used as neutraceuticals.
An analytical technique for the determination of volatile phytochemical compounds by GC-MS in oni... more An analytical technique for the determination of volatile phytochemical compounds by GC-MS in onion, Allium cepa L., was reported here. Three different cultivars of onion bulb (O1, O2 and O3) were extracted with petroleum ether, and the volatile compounds were analyzed by GC-MS. Terpenes such as humulaneand phytosterols and aldehydes are found common in all the three cultivars. Thiophenes and disulphides were identified as major compounds in O1 and O3 respectively. Caryophyllene, a terpene was observed in both O1 and O2. Squalene, allicin and sulphide compounds were identified specifically in O3. The study further reported that the composition of volatile compounds in the cultivars is varied and these differences may be due to the variations in geographic, climatic, soil fertility and cultivation practices. The intensity of flavour is governed by hereditary factors and environmental conditions under which the onions grow.
Conference Presentations by T. Mini Shobi
The aim of the present study was to optimize the culture medium components in the growth media of... more The aim of the present study was to optimize the culture
medium components in the growth media of
Begonia malabarica in order to enhance the shoot
multiplications for extends its applicability to the
commercial production of secondary metabolites.
Optimization of medium components such as sucrose,
6-benzylaminopurine (BAP) and 1-naphthaleneacetic
acid (NAA) was performed by Central Composite
Design- Response Surface Methodology (CCD-RSM)
to induce shoot multiplication. The maximum shoot
multiplication were found to be Murashige and Skoog
(MS) basal medium supplemented with 30 g/l of sucrose,
0.5 mg/l of BAP and 0.55 mg/l of NAA with 8.9
number of bud per explant. It was observed that the
model predicted values of almost all points were nearer
to experimental values. The fully grown explants were
transferred to rooting medium at the concentration of
0.5 mg/l NAA. The rooted plantlets were acclimatized
with sand, garden soil and farmyard manure at the
ratio of 1:1:1 in environmental growth chamber. The
present work reveals shoot multiplication efficacy on MS medium at optimized concentrations of growth
regulators without expending more media constituents.
Natural products from medicinal plants either as pure compounds or as standardized extracts provi... more Natural products from medicinal plants either as pure
compounds or as standardized extracts provide unlimited
opportunities for new drug leads because of
the unmatched availability of chemical diversity. Begonia
malabarica Lam. is one of the medicinally important
herbs belonging to the family Begoniaceae.
It is reported that the leaves are used to treat respiratory
infections, diarrhea, blood cancer and skin
diseases. The whole plant possesses a variety of secondary
metabolites. Separation of these compounds
adds more novel (Biological active) structures to
screen lead compounds in modern drug industries.
In this line, the compound from Begonia malabarica
was extracted and purified by various solvent extraction
methods and different chromatographic techniques. The colorless or pale yellow oily nature
compound was isolated which is soluble in chloroform.
Further, the structure was elucidated by comparing
the spectral data of FT-IR, MS, 1H-NMR, and
13C-NMR. This is the first report of di-butyl phthalate,
isolated from the family of Begoniaece which exhibited
potential antibacterial and anticancer activities.
Begonia malabarica Lam. belongs to the family of Begoniaceae and is distributed in peninsular Ind... more Begonia malabarica Lam. belongs to the family of Begoniaceae and is distributed in
peninsular India and Sri Lanka. In Tamil, it is called as Narayanachanjeevi, Sengurungu and
Senthandu. The Paliyan tribe of Tirunelveli District in Tamil Nadu substitutes its leaves for
Tamarind (Tamarindus indica L., Caesalpinoideae) and consume after cooking. Further, they
consume boiled leaves for stomachache, stomach ulcer, and respiratory problems. Previously, it
is reported that the leaves are used to treat respiratory infections, diarrhea, blood cancer and skin
diseases. Compounds reported include friedelin, epi-friedelinol, β-sitosterol, luteolin, quercetin
and β-stitosterol-3-β-D-glucopyranoside. Successive solvent extracts (petroleum ether,
chloroform, ethyl acetate and methanol) of the leaves, stem and roots were tested for preliminary
phytochemical analysis which indicated the presence of steroids in chloroform extract of the
leaves, petroleum ether, chloroform and methanol extracts of the stem, triterpenoids in
petroleum ether and methanol extracts of the leaves and methanol extract of the root, reducing
sugars in methanol extract of leaves and ethyl acetate extract of the root, sugars in all extracts of
the leaves and petroleum ether extract of the root, alkaloids in methanol extract of the leaves and
chloroform and methanol extracts of the stem, flavonoids in ethyl acetate and methanol extracts
Asian Journal of Pharmaceutical and Clinical Research, 2015
Objective: This study was subjected to analyse the technique for the determination of quercetin, ... more Objective: This study was subjected to analyse the technique for the determination of quercetin, a flavonoid in onion ( Allium cepa L.) from three different cultivars of bulb onion (O1, O2 and O3) which were extracted with petroleum ether, chloroform, methanol and water. Also this study was aimed to determine the antimicrobial activity of four solvent extracts of three onion cultivars against ten MTCC bacterial pathogens. Methods: Quercetin in onion was determined by analytical technique, HPTLC and the antimicrobial activity was performed by well diffusion method. Result: Quercetin was determined in all the three cultivars with Rf value 0.68. Quercetin was also extracted in all the four solvent extracts of onion cultivars. Apart from quercetin, few more compounds also isolated. More compounds were isolated in O3. In the determination of antimicrobial activity of onion extracts against MTCC cultures revealed that all the three onion cultivars were highly active against Staphylococc...
International Journal of Current Research in Biosciences and Plant Biology, 2017
An analytical technique for the determination of volatile phytochemical compounds by GC-MS in oni... more An analytical technique for the determination of volatile phytochemical compounds by GC-MS in onion, Allium cepa L., was reported here. Three different cultivars of onion bulb (O1, O2 and O3) were extracted with petroleum ether, and the volatile compounds were analyzed by GC-MS. Terpenes such as humulaneand phytosterols and aldehydes are found common in all the three cultivars. Thiophenes and disulphides were identified as major compounds in O1 and O3 respectively. Caryophyllene, a terpene was observed in both O1 and O2. Squalene, allicin and sulphide compounds were identified specifically in O3. The study further reported that the composition of volatile compounds in the cultivars is varied and these differences may be due to the variations in geographic, climatic, soil fertility and cultivation practices. The intensity of flavour is governed by hereditary factors and environmental conditions under which the onions grow.
GSC Biological and Pharmaceutical Sciences, 2018
A b s t r a c t A r t i c l e I n f o Begonia fallax A.DC. belongs to the family of Begoniaceae a... more A b s t r a c t A r t i c l e I n f o Begonia fallax A.DC. belongs to the family of Begoniaceae and is an endemic species to Peninsular India. Shoot tip and nodal explants were screened using Murashige and Skoog medium supplemented with BAP, KIN and TDZ individually and combination with IAA, NAA and IBA for multiple shoot initiation. BAP and NAA (0.5+0.5 mg-l) produced 20.8 shoots with up to 4.0 cm shoot length to nodal explants whereas 17.9 shoots with 3.1 cm shoot length to shoot tip explants. For root induction, various concentrations of IAA, NAA and IBA were used individually. The highest rooting frequency was achieved to nodal explants (64.90 roots) on MS medium supplemented with 0.5 mg-l NAA compared to shoot tip explants (31.90 roots). Invariably, root length was up to 2.3 cm to both the explants. The rooted explants were transplanted into plastic cups and showed 70% survival rate in the Environmental Growth Chamber. Nodal explants could be the best for large-scale multiplication and propagation to facilitate in vitro conservation and for secondary metabolites production.
Garlic, Allium sativum L. is a plant with so many medicinal properties and as therapeutic agent w... more Garlic, Allium sativum L. is a plant with so many medicinal properties and as therapeutic agent
with a peculiar odor. It was used as antiseptic to prevent gangrene during the two world wars and was
ingested as stimulant by the athletes during the first Olympic Games in Greece. An antiseptic lotion
prepared out of it is used to clean wounds and ulcers in India. Onion and garlic tea is suggested to treat
fever, headache, cholera and dysentery in China. Due to its medicinal property, it is used to treat many
disorders right from respiratory ailments, asthma, pneumonia, diabetes, cardiovascular disorders to
rheumatism. The medicinal property is due to the presence of a large number of bioactive compounds.
The unique odour is attributed for the presence of organosulfur compounds which can be analyzed by
GC-MS. Major bioactive components identified in the present study are allicin, diallyl disulfide, allyl
alcohol, alliin, diallylsulphide, dimethyl sulphate, dimethyl disulfide, diallyl ether, allyl methyl disulfide,
allyl propyl sulphide, dimethyl propyl disulfide, dipropyl disulfide, allyl methyl sulphide and
trichloroethylene.
Objective: This study was subjected to analyze the technique for the determination of quercetin, ... more Objective: This study was subjected to analyze the technique for the determination of quercetin, a flavonoid in onion (Allium cepa L.) from three different cultivars of bulb onion (O1, O2, and O3) which were extracted with petroleum ether, chloroform, methanol, and water. Furthermore, this study was aimed to determine the antimicrobial activity of four solvent extracts of three onion cultivars against 10 microbial type culture collection (MTCC) bacterial pathogens.
Methods: Quercetin in onion was determined by analytical technique, high-performance thin layer chromatography, and the antimicrobial activity were performed by the well diffusion method.
Result: Quercetin was determined in all the three cultivars with retention factor value 0.68. Quercetin was also extracted in all the four solvent extracts of onion cultivars. Apart from quercetin, few more compounds also isolated. More compounds were isolated in O3. In the determination of the antimicrobial activity of onion extracts against MTCC cultures revealed that all the three onion cultivars were highly active against Staphylococcus aureus, it showed more than 25 mm zone of inhibition. Among the three onion cultivars, O2 showed better antimicrobial activity against all the tested bacterial cultures.
Conclusion: This study showed that onion has good broad spectrum bioactivity against microorganisms revealed that it contains phytochemicals which can be used as nutraceuticals.
This study was subjected to analyse the technique for the determination of quercetin, a flavonoid... more This study was subjected to analyse the technique for the determination of quercetin, a flavonoid in garlic (Allium
sativum L.) from three different cultivars of bulb onion (G1, G2 and G3) which were extracted with petroleum ether,
chloroform, methanol and water. Also this study was aimed to determine the antimicrobial activity of four solvent
extracts of garlic cultivar against ten MTCC bacterial pathogens. Quercetin in garlic was determined by analytical
technique, HPTLC and the antimicrobial activity was performed by well diffusion method. Quercetin was
determined in all the three cultivars with Rf value 0.68. Quercetin was also extracted in all the four solvent extracts
of garlic cultivars. Apart from quercetin, few more compounds also isolated. More compounds were isolated in G1.
In the determination of antimicrobial activity of onion extracts against MTCC cultures revealed that onion cultivar
was highly active against Staphylococcus aureus, it showed more than 25mm zone of inhibition. This study showed
that onion has good broad spectrum bioactivity against microorganisms revealed that it contains phytochemicals
which can be used as neutraceuticals.
An analytical technique for the determination of volatile phytochemical compounds by GC-MS in oni... more An analytical technique for the determination of volatile phytochemical compounds by GC-MS in onion, Allium cepa L., was reported here. Three different cultivars of onion bulb (O1, O2 and O3) were extracted with petroleum ether, and the volatile compounds were analyzed by GC-MS. Terpenes such as humulaneand phytosterols and aldehydes are found common in all the three cultivars. Thiophenes and disulphides were identified as major compounds in O1 and O3 respectively. Caryophyllene, a terpene was observed in both O1 and O2. Squalene, allicin and sulphide compounds were identified specifically in O3. The study further reported that the composition of volatile compounds in the cultivars is varied and these differences may be due to the variations in geographic, climatic, soil fertility and cultivation practices. The intensity of flavour is governed by hereditary factors and environmental conditions under which the onions grow.
The aim of the present study was to optimize the culture medium components in the growth media of... more The aim of the present study was to optimize the culture
medium components in the growth media of
Begonia malabarica in order to enhance the shoot
multiplications for extends its applicability to the
commercial production of secondary metabolites.
Optimization of medium components such as sucrose,
6-benzylaminopurine (BAP) and 1-naphthaleneacetic
acid (NAA) was performed by Central Composite
Design- Response Surface Methodology (CCD-RSM)
to induce shoot multiplication. The maximum shoot
multiplication were found to be Murashige and Skoog
(MS) basal medium supplemented with 30 g/l of sucrose,
0.5 mg/l of BAP and 0.55 mg/l of NAA with 8.9
number of bud per explant. It was observed that the
model predicted values of almost all points were nearer
to experimental values. The fully grown explants were
transferred to rooting medium at the concentration of
0.5 mg/l NAA. The rooted plantlets were acclimatized
with sand, garden soil and farmyard manure at the
ratio of 1:1:1 in environmental growth chamber. The
present work reveals shoot multiplication efficacy on MS medium at optimized concentrations of growth
regulators without expending more media constituents.
Natural products from medicinal plants either as pure compounds or as standardized extracts provi... more Natural products from medicinal plants either as pure
compounds or as standardized extracts provide unlimited
opportunities for new drug leads because of
the unmatched availability of chemical diversity. Begonia
malabarica Lam. is one of the medicinally important
herbs belonging to the family Begoniaceae.
It is reported that the leaves are used to treat respiratory
infections, diarrhea, blood cancer and skin
diseases. The whole plant possesses a variety of secondary
metabolites. Separation of these compounds
adds more novel (Biological active) structures to
screen lead compounds in modern drug industries.
In this line, the compound from Begonia malabarica
was extracted and purified by various solvent extraction
methods and different chromatographic techniques. The colorless or pale yellow oily nature
compound was isolated which is soluble in chloroform.
Further, the structure was elucidated by comparing
the spectral data of FT-IR, MS, 1H-NMR, and
13C-NMR. This is the first report of di-butyl phthalate,
isolated from the family of Begoniaece which exhibited
potential antibacterial and anticancer activities.
Begonia malabarica Lam. belongs to the family of Begoniaceae and is distributed in peninsular Ind... more Begonia malabarica Lam. belongs to the family of Begoniaceae and is distributed in
peninsular India and Sri Lanka. In Tamil, it is called as Narayanachanjeevi, Sengurungu and
Senthandu. The Paliyan tribe of Tirunelveli District in Tamil Nadu substitutes its leaves for
Tamarind (Tamarindus indica L., Caesalpinoideae) and consume after cooking. Further, they
consume boiled leaves for stomachache, stomach ulcer, and respiratory problems. Previously, it
is reported that the leaves are used to treat respiratory infections, diarrhea, blood cancer and skin
diseases. Compounds reported include friedelin, epi-friedelinol, β-sitosterol, luteolin, quercetin
and β-stitosterol-3-β-D-glucopyranoside. Successive solvent extracts (petroleum ether,
chloroform, ethyl acetate and methanol) of the leaves, stem and roots were tested for preliminary
phytochemical analysis which indicated the presence of steroids in chloroform extract of the
leaves, petroleum ether, chloroform and methanol extracts of the stem, triterpenoids in
petroleum ether and methanol extracts of the leaves and methanol extract of the root, reducing
sugars in methanol extract of leaves and ethyl acetate extract of the root, sugars in all extracts of
the leaves and petroleum ether extract of the root, alkaloids in methanol extract of the leaves and
chloroform and methanol extracts of the stem, flavonoids in ethyl acetate and methanol extracts