Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation (original) (raw)

References

  1. Hershko, A., and Ciechanover, A. (1998) The ubiquitin system. Annu Rev Biochem 67, 425–479.
    Article PubMed CAS Google Scholar
  2. Hochstrasser, M. (1996) Ubiquitin-depend-ent protein degradation. Annu Rev Genet 30, 405–439.
    Article PubMed CAS Google Scholar
  3. Johnson, E. S. (2004) Protein modification by SUMO. Annu Rev Biochem 73, 355–382.
    Article PubMed CAS Google Scholar
  4. Laney, J. D., and Hochstrasser, M. (1999) Substrate targeting in the ubiquitin system. Cell 97, 427–430.
    Article PubMed CAS Google Scholar
  5. Melchior, F. (2000) SUMO–nonclassical ubiq-uitin. Annu Rev Cell Dev Biol 16, 591–626.
    Article PubMed CAS Google Scholar
  6. Muller, S., Hoege, C., Pyrowolakis, G., and Jentsch, S. (2001) SUMO, ubiquitin's mysterious cousin. Nat Rev Mol Cell Biol 2, 202–210.
    Article PubMed CAS Google Scholar
  7. Bernier-Villamor, V., Sampson, D. A., Matunis, M. J., and Lima, C. D. (2002) Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1. Cell 108, 345–356.
    Article PubMed CAS Google Scholar
  8. Yunus, A. A., and Lima, C. D. (2006) Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway. Nat Struct Mol Biol 13, 491–499.
    Article PubMed CAS Google Scholar
  9. Cheng, C. H., Lo, Y. H., Liang, S. S., Ti, S. C., Lin, F. M., Yeh, C. H., Huang, H. Y., and Wang, T. F. (2006) SUMO modifications control assembly of synaptonemal complex and polycomplex in meiosis of Saccharomyces cerevisiae. Genes Dev 20, 2067–2081.
    Article PubMed CAS Google Scholar
  10. Hochstrasser, M. (2001) SP-RING for SUMO: new functions bloom for a ubiqui-tin-like protein. Cell 107, 5–8.
    Article PubMed CAS Google Scholar
  11. Johnson, E. S., and Gupta, A. A. (2001) An E3-like factor that promotes SUMO conjugation to the yeast septins. Cell 106, 735–744.
    Article PubMed CAS Google Scholar
  12. Kahyo, T., Nishida, T., and Yasuda, H. (2001) Involvement of PIAS1 in the sumoylation of tumor suppressor p53. Mol Cell 8, 713–718.
    Article PubMed CAS Google Scholar
  13. Potts, P. R., and Yu, H. (2005) Human MMS21/NSE2 is a SUMO ligase required for DNA repair. Mol Cell Biol 25, 7021–7032.
    Article PubMed CAS Google Scholar
  14. Schmidt, D., and Muller, S. (2003) PIAS/ SUMO: new partners in transcriptional regulation. Cell Mol Life Sci 60, 2561–2574.
    Article PubMed CAS Google Scholar
  15. Takahashi, Y., Toh-e, A., and Kikuchi, Y. (2001) A novel factor required for the SUMO1/Smt3 conjugation of yeast septins. Gene 275, 223–231.
    Article PubMed CAS Google Scholar
  16. Zhao, X., and Blobel, G. (2005) A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization. Proc Natl Acad Sci U S A 102, 4777–4782.
    Article PubMed CAS Google Scholar
  17. Pichler, A., Gast, A., Seeler, J. S., Dejean, A., and Melchior, F. (2002) The nucleop-orin RanBP2 has SUMO1 E3 ligase activity. Cell 108, 109–120.
    Article PubMed CAS Google Scholar
  18. Pichler, A., Knipscheer, P., Saitoh, H., Sixma, T. K., and Melchior, F. (2004) The RanBP2 SUMO E3 ligase is neither HECT- nor RING-type. Nat Struct Mol Biol 11, 984–991.
    Article PubMed CAS Google Scholar
  19. Reverter, D., and Lima, C. D. (2005) Insights into E3 ligase activity revealed by a SUMO-RanGAP1-Ubc9-Nup358 complex. Nature 435, 687–692.
    Article PubMed CAS Google Scholar
  20. Mossessova, E., and Lima, C. D. (2000) Ulp1-SUMO crystal structure andgenetic analysis reveal conserved interactions and a regulatory element essential for cellgrowth in yeast. Mol Cell 5, 865–876.
    Article PubMed CAS Google Scholar
  21. Hoege, C., Pfander, B., Moldovan, G. L., Pyrowolakis, G., and Jentsch, S. (2002) RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO. Nature 419, 135–141.
    Article PubMed CAS Google Scholar
  22. Pfander, B., Moldovan, G. L., Sacher, M., Hoege, C., and Jentsch, S. (2005) SUMO-modified PCNA recruits Srs2 to prevent recombination during S phase. Nature 436, 428–433.
    PubMed CAS Google Scholar
  23. Fersht, A. (2000) The pH dependence of enzyme catalysis. in Structure and Mechanism in Protein Science, W.H Freeman and Company, New York.
    Google Scholar

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