Relationship between pyrophosphate, amorphous calcium phosphate and other factors in the sequence of calcificationin vivo (original) (raw)

Abstract

The amounts of inorganic pyrophosphate (PPi), orthophosphate and calcium have been measured in resting, proliferating, hypertrophic and calcified cartilage from foetal calf epiphyses and also in cancellous, periosteal and compact bone. In the cartilage samples, the content of PPi increased progressively in the order named above, from values of 8.59 μg P/g dry weight in resting cartilage to 236 μg P/g dry weight in calcified cartilage. However, the ratio of PPi to orthophosphate followed the reverse relationship and was highest in the resting zone and fell dramatically as the tissue calcified. The possible role of PPi in inhibiting the precipitation of amorphous calcium phosphate (ACP) and in the slowing the transformation of ACP to hydroxyapatite (HA) in calcifying tissues is discussed in relation to other factors, such as collagen, magnesium, phospholipids and proteinpolysaccharides, which might also influence the process_in vivo_. At present, no single factor can be identified as a proven physiological regulator.

Résumé

Les concentrations de pyrophosphate inorganiques (PPi), d'orthophosphate et de calcium ont été déterminées dans le cartilage au repos, en prolifération, hypertrophique et calcifié au niveau d'épiphyses foetales de veaux et aussi dans l'os spongieux, périosté et compact. Dans les échantillons de cartilage, le contenu en PPi augmente progressivement dans l'ordre énoncé ci-dessus, de 8.59 μg P/g en poids sec de cartilage au repos à 236 μg P/g en poids sec dans le cartilage calcifié. Cependant, le rapport PPi sur orthophosphate suit une relation inverse: il est plus élevé dans la zone de repos et diminue considérablement lorsque le tissu se calcifie. Le rôle possible du PPi, en inhibant la précipitation de phosphate de calcium amorphe (ACP) et en ralentissant la transformation d'ACP en hydroxyleapatite (HA) dans les tissus calcifiés, est envisagé par rapport à d'autres facteurs, tels que le collagène, le magnésium, les phospholipides et les protéines-polysaccharides, qui peuvent aussi intervenir_in vivo_. Pour l'instant, aucun facteur isolé ne peut être considéré comme un régulateur physiologique certain.

Zusammenfassung

Der Gehalt an anorganischem Pyrophosphat (PPi), Orthophosphat und Calcium wurde in ruhendem, proliferierendem, hypertrophischem und verkalktem Knorpel von foetalen Kalbsepiphysen und auch in spongiösem, periostalem und kompaktem Knochen gemessen. Bei den Knorpelproben nahm der Gehalt an PPi progressiv in der oben angegebenen Reihenfolge zu: von 8,59 μg P/g Trockengewicht in ruhendem Knorpel bis zu 236 μg P/g Trockengewicht in verkalktem Knorpel. Das PPi/Orthophosphat-Verhältnis hingegen verlief in umgekehrter Richtung; es war am höchsten in der ruhenden Zone und nahm dramatisch ab, wenn das Gewebe verkalkte. Die mögliche Rolle von PPi bei der Hemmung der Ausfällung von amorphem Calciumphosphat (ACP) und bei der Verlangsamung der Umwandlung von ACP in Hydroxyapatit (HA) in verkalkenden Geweben wird in bezug auf folgende anderen Faktoren, welche den Vorgang in vivo ebenfalls beieinflussen könnten, diskutiert: Collagen, Magnesium, Phospholipide und Proteinpolysaccharide. Bis jetzt war es nicht möglich, einen einzelnen Faktor als sicheren physiologischen Regulator zu identifizieren.

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Authors and Affiliations

1. Department of Biochemistry and Orthopaedic Surgery, University of Vermont, College of Medicine, Burlington, Vermont
   R. E. Wuthier Ph.D.
2. Department of Pathophysiology, University of Berne Laboratory for Experimental Surgery, Davos-Platz
   S. Bisaz, R. G. G. Russell & H. Fleisch
3. Department of Orthopaedic Surgery, University of Vermont, College of Medicine, 05401, Burlington, Vermont, USA
   R. E. Wuthier Ph.D.

Authors

1. R. E. Wuthier Ph.D.
2. S. Bisaz
3. R. G. G. Russell
4. H. Fleisch

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Wuthier, R.E., Bisaz, S., Russell, R.G.G. et al. Relationship between pyrophosphate, amorphous calcium phosphate and other factors in the sequence of calcification_in vivo_.Calc. Tis Res. 10, 198–206 (1972). https://doi.org/10.1007/BF02012549

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• Received: 21 January 1972
• Accepted: 14 April 1972
• Issue date: December 1972
• DOI: https://doi.org/10.1007/BF02012549

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