Specific detection and semi-quantitative analysis of TRPC4 protein expression by antibodies (original) (raw)

References

  1. Corey DP, Garcia-Añoveros J, Holt JR, Kwan KY, Lin SY, Vollrath MA, Amalfitano A, Cheung ELM, Derfler BH, Duggan A, Géléoc GSG, Gray PA, Hoffmann MP, Rehm HL, Tamasauskas D, Zhang DS (2004) TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells. Nature 432:723–730
    Article CAS PubMed Google Scholar
  2. Montell C, Birnbaumer L, Flockerzi V (2002) The TRP channels, a remarkably functional family. Cell 108:595–598
    CAS PubMed Google Scholar
  3. Wissenbach U, Niemeyer BA, Himmerkus N, Fixemer T, Bonkhoff H, Flockerzi V (2004) TRPV6 and prostate cancer: cancer growth beyond the prostate correlates with increased TRPV6 Ca2+ channel expression. Biophys Biochem Res Com 322:359–1363
    Google Scholar
  4. Birnbaumer L, Boulay G, Brown D, Jiang M, Dietrich A, Mikoshiba K, Zhu X, Qin N (2000) Mechanism of capacitative Ca2+ entry (CCE): interaction between IP3 receptor and TRP links the internal calcium storage compartment to plasma membrane CCE channels. Recent Prog Horm Res 55:127–162
    CAS PubMed Google Scholar
  5. Kiselyov KI, Shin DM, Wang Y, Pessah IN, Allen PD, Muallem S (2000) Gating of store-operated channels by conformational coupling to ryanodine receptors. Mol Cell 6:421–431
    CAS PubMed Google Scholar
  6. Hofmann T, Obukhov AG, Schaefer M, Harteneck C, Gudermann T, Schultz G (1999) Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. Nature 397:259–263
    Article CAS PubMed Google Scholar
  7. Philipp S, Cavalié A, Freichel M, Wissenbach U, Zimmer S, Trost C, Marquart A, Murakami M, Flockerzi V (1996) A mammalian capacitative calcium entry channel homologous to Drosophila TRP and TRPL. EMBO J 15:6166–6171
    CAS PubMed Google Scholar
  8. Warnat J, Philipp S, Zimmer S, Flockerzi V, Cavalié A (1999) Phenotype of a recombinant store-operated channel: highly selective permeation of Ca2+. J Physiol (Lond) 518:631–638
    Google Scholar
  9. Philipp S, Trost C, Warnat J, Rautmann J, Himmerkus N, Schroth G, Kretz O, Nastainczyk W, Cavalié A, Hoth M, Flockerzi V (2000) TRP4 (CCE1) Protein is part of native calcium release-activated Ca2+-like channels in adrenal cells. J Biol Chem 275:23965–23972
    Article CAS PubMed Google Scholar
  10. Freichel M, Suh SH, Pfeifer A, Schweig U, Trost C, Weißgerber P, Biel M, Philipp S, Freise D, Droogmans G, Hofmann F, Flockerzi V, Nilius B (2001) Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4−/− mice. Nat Cell Biol 3:121–127
    Article CAS PubMed Google Scholar
  11. Vazquez G, Lievremont JP, Bird GSJ, Putney JW (2001) Human Trp3 forms both inositol trisphosphate receptor-dependent and receptor-independent store-operated cation channels in DT40 avian B lymphocytes. Proc Natl Acad Sci 98:11777–11782
    Article CAS PubMed Google Scholar
  12. Zitt C, Zobel A, Obukhov AG, Harteneck C, Kalkbrenner F, Lückhoff A, Schultz G (1996) Cloning and functional expression of a human Ca2+-permeable cation channel activated by calcium store depletion. Neuron 16:1189–1196
    Article CAS PubMed Google Scholar
  13. van Rossum DB, Patterson RL, Sharma S, Barrow RK, Kornberg M, Gill DL, Snyder SH (2005) Phospholipase Cγ1 controls surface expression of TRPC3 through an intermolecular PH domain. Nature 434:99–104
    Google Scholar
  14. Singh BB, Lockwich TP, Bandyopadhyay BC, Liu X, Bollimuntha S, Brazer S, Combs C, Das S, Leenders M, Sheng ZH, Knepper MA, Ambudkar SV, Ambudkar IS (2004) VAMP2-dependent exocytosis regulates plasma membrane insertion of TRPC3 channels and contributes to agonist-stimulated Ca2+ influx. Mol Cell 15:635–646
    Article CAS PubMed Google Scholar
  15. Bezzerides VJ, Ramsey IS, Kotecha S, Greka A, Clapham DE (2004) Rapid vesicular translocation and insertion of TRP channels. Nat Cell Biol 6:709–720
    Article CAS PubMed Google Scholar
  16. Maroto R, Raso A, Wood TG, Kurosky A, Martinac B, Hamill OP (2005) TRPC1 forms the stretch-activated cation channel in vertebrate cells. Nat Cell Biol 7:179–185
    Article CAS PubMed Google Scholar
  17. Trost C, Bergs C, Himmerkus N, Flockerzi V (2001) The transient receptor potential, TRP4, cation channel is a novel member of the family of calmodulin binding proteins. Biochem J 355:663–670
    CAS PubMed Google Scholar
  18. Tang J, Lin Y, Zhang Z, Tikunova S, Birnbaumer L, Zhu MX (2001) Identification of common binding sites for calmodulin and inositol 1,4,5-trisphosphate receptors on the carboxyl termini of trp channels. J Biol Chem 276:21303–21310
    Article CAS PubMed Google Scholar
  19. Mery L, Magnino F, Schmidt K, Krause KH, Dufour JF (2001) Alternative splice variants of hTrp4 differentially interact with the C-terminal portion of the inositol 1,4,5-trisphosphate receptors. FEBS Lett 487:377–383
    Article CAS PubMed Google Scholar
  20. Zhang Z, Tang J, Tikunova S, Johnson JD, Chen Z, Qin N, Dietrich A, Stefani E, Birnbaumer L, Zhu MX (2000) Activation of Trp3 by inositol 1,4,5-trisphosphate receptors through displacement of inhibitory calmodulin from a common binding domain. Proc Natl Acad Sci 98:3168–3173
    Article Google Scholar
  21. Vandebrouck C, Martin D, Colson-Van Schorr M, Debaix H, Gailly P (2002) Involvement of TRPC in the abnormal calcium influx observed in dystrophic (mdx) mouse skeletal muscle fibers. J Cell Biol 158:1089–1096
    Article CAS PubMed Google Scholar
  22. Weigl L, Zidar A, Gscheidlinger R, Karel A, Hohenegger M (2003) Store-operated Ca2+ influx by selective depletion of ryanodine-sensitive Ca2+ pools in primary human skeletal muscle cells. Naunyn Schmiedeberg’s Arch Pharmacol 367:353–363
    CAS Google Scholar
  23. Freichel M, Wissenbach U, Philipp S, Flockerzi V (1998) Alternative splicing and tissue specific expression of the 5′ truncated bCCE 1 variant bCCE 1Δ 514. FEBS Lett 422:354–358
    Article CAS PubMed Google Scholar
  24. Maurer P, Moratzky A, Fecher-Trost C, Flockerzi V, Lenk U, Sommer T, Völzing C, Zimmermann R (2003) Cell-free synthesis of membrane proteins on a preparative scale. In: Swartz JR (ed) Cell-free protein expression. Springer, Berlin Heidelberg New York, pp 133–139
    Google Scholar
  25. Yellen G (2002) The voltage-gated potassium channels and their relatives. Nature 419:35–42
    Google Scholar
  26. Ong HL, Chen J, Chataway T, Brereton H, Zhang L, Downs T, Tsiokas L, Barritt G (2002) Specific detection of the endogenous transient receptor potential (TRP)-1 protein in liver and airway smooth muscle cells using immunoprecipitation and Western-blot analysis. Biochem J 364:641–648
    Article CAS PubMed Google Scholar
  27. Reuter H (1983) Calcium channel modulation by neurotransmitters, enzymes and drugs. Nature 301:569–574
    Google Scholar
  28. Bosse E, Bottlender R, Kleppisch T, Hescheler J, Welling A, Hofmann F, Flockerzi V (1992) Stable and functional expression of the calcium channel α1 subunit from smooth muscle in somatic cell lines. EMBO J 11:2033–2038
    CAS PubMed Google Scholar
  29. Flockerzi V, Oeken HJ, Hofmann F, Pelzer D, Cavalié A, Trautwein W (1986) Purified dihydropyridine-binding site from skeletal muscle t-tubules is a functional calcium channel. Nature 323:66–68
    Google Scholar
  30. Lockwich TP, Liu X, Singh BB, Jadlowiec J, Weiland S, Ambudkar IS (2000) Assembly of Trp1 in a signalling complex associated with caveolin-scaffolding lipid raft domains. J Biol Chem 275:11934–11942
    CAS PubMed Google Scholar
  31. Murakami M, Fleischmann B, DeFelipe C, Freichel M, Trost C, Ludwig A, Wissenbach U, Schwegler H, Hofmann F, Hescheler J, Flockerzi V, Cavalié A (2002) Pain perception in mice lacking the β3 subunit of voltage-activated calcium channels. J Biol Chem 277:40342–40351
    Google Scholar
  32. Berggren PO, Yang SN, Murakami M, Efanov AM, Uhles S, Köhler M, Moede T, Fernström A, Appelskog IB, Aspinwall CA, Zaitsev SV, Larsson O, de Vargas LM, Fecher-Trost C, Weißgerber P, Ludwig A, Leibiger B, Juntti-Berggren L, Barker CJ, Gromada J, Freichel M, Leibiger IB, Flockerzi V (2004) Removal of Ca2+ channel β3 subunit enhances Ca2+ oscillation frequency and insulin exocytosis. Cell 119: 273–284
    Article CAS PubMed Google Scholar
  33. Niemeyer BA (2005) Structure-function analysis of TRPV channels. Naunyn Schmiedeberg’s Arch Pharmacol DOI: 10.1007/s00210-005-1053-7
  34. Nicke A, Rettinger J, Schmalzing G (2003) Monomeric and dimeric byproducts are the principal functional elements of higher order P2X1 concatamers. Mol Pharmacol 63:243–252
    Article CAS PubMed Google Scholar

Download references