Effects of integrins on laminin chemotaxis by hepatocellular carcinoma cells (original) (raw)

Abstract

To quantitatively evaluate the effects of integrins α1β1, α2β1, α3β1, α4β1, α5β1, and α6β1 on the chemotaxis of hepatocelluar carcinoma (HCC) cell line SMMC-7721 to laminin (LN). A modified dual-micropipette system was used to dynamically and quantitatively monitor the formation of pseudopod protrusion of HCC cells toward LN in the presence or absence of specific antibodies against integrins α1, α2, α3, α4, α5, α6, and β1. Additionally, the expression levels of different integrin subunits on the surface of the cells were determined via flow cytometry analysis. In response to equal concentrations of LN in both micropipettes, HCC cells form symmetrical pseudopod protrusions on both sides. Addition of antibodies against α3, α6, or β1 into one micropipette leads to significant reduction of pseudopod formation on that side, while antibodies against α1, α2, α4, and α5 do not affect the symmetrical formation of pseudopods in either micropipette. The percentages of HCC cells positive for expression of integrins α1, α2, α3, α4, α5, α6, and β1 were 95.07, 23.17, 95.55, 2.47, 34, 14.29, and 95.78%, respectively. Integrins α3β1 and α6β1 are important cell surface receptors that mediate the chemotaxis of HCC cells toward LN.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Project no. 39970198) and the Visiting Scholar Foundation of Key Laboratory in Chongqing University, Ministry of Education, China ([2002] 4).

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Authors and Affiliations

  1. College of Resources and Environmental Sciences, Chongqing University, 400044, Chongqing, China
    Bian-Hong Fu
  2. Key Laboratory for Biomechanics and Tissue Engineering of Ministry of Education, Bioengineering College of Chongqing University, 400044, Chongqing, China
    Ze-Zhi Wu & Jian Qin

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  1. Bian-Hong Fu
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  2. Ze-Zhi Wu
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  3. Jian Qin
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Correspondence toBian-Hong Fu.

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Fu, BH., Wu, ZZ. & Qin, J. Effects of integrins on laminin chemotaxis by hepatocellular carcinoma cells.Mol Biol Rep 37, 1665–1670 (2010). https://doi.org/10.1007/s11033-009-9790-1

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