Biosynthesis of silver nanoparticles by a new strain of Streptomyces sp. compared with Aspergillus fumigatus (original) (raw)

Abstract

Locally isolated strains of a thermoalkalotolerant Streptomyces sp. and Aspergillus fumigatus were used for the in vitro biosynthesis of silver nanoparticles from AgNO3 solutions. An autolysed cell-free culture filtrate from each strain was used, indicating that the formation mechanism depends on intra-cellular components for both organisms, since culture broths had no significant nanoparticle formation potential. Nanoparticle formation was indicated by a change of the solution from colourless or light brown to dark brown after 24 h or more, and UV–visible spectroscopy and x-ray diffraction analysis confirmed the formation by both organisms. The initial formation kinetics were faster with Aspergillus, but formation continued for a longer period with Streptomyces, resulting in higher concentrations after 48 h. Transmission electron microscope images revealed well dispersed nanoparticles with diameters ranging from 15 to 45 nm from A. fumigatus, while those from Streptomyces sp. had a narrower size distribution of 15–25 nm. The higher productivity and preferred narrower size distribution of Streptomyces, together with its well established industrial use, may make it the preferred choice for further optimization studies.

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Acknowledgments

Portions of this work were funded by the Natural Sciences and Engineering Research Council of Canada.

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Authors and Affiliations

1. Engineering Technology, Biotechnology Program, McMaster University, Hamilton, ON, Canada
   Faiez Alani
2. Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada
   Murray Moo-Young & William Anderson

Authors

1. Faiez Alani
2. Murray Moo-Young
3. William Anderson

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Correspondence toWilliam Anderson.

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Alani, F., Moo-Young, M. & Anderson, W. Biosynthesis of silver nanoparticles by a new strain of Streptomyces sp. compared with Aspergillus fumigatus .World J Microbiol Biotechnol 28, 1081–1086 (2012). https://doi.org/10.1007/s11274-011-0906-0

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• Received: 04 February 2011
• Accepted: 19 September 2011
• Published: 04 October 2011
• Issue date: March 2012
• DOI: https://doi.org/10.1007/s11274-011-0906-0

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