Pravin Mishra | University of Hawaii at Manoa (original) (raw)

Papers by Pravin Mishra

Encyclopedia, 2021

Capture myopathy is a noninfectious disease of the muscle associated with capturing or handling o... more Capture myopathy is a noninfectious disease of the muscle associated with capturing or handling of any animals.

Foods, 2021

Natural colorants have been used in several ways throughout human history, such as in food, dyes,... more Natural colorants have been used in several ways throughout human history, such as in food, dyes, pharmaceuticals, cosmetics, and many other products. The study aimed to isolate the natural colorant-producing filamentous fungi Aspergillus niger from soil and extract pigments for its potential use specially for food production. Fourteen soil samples were collected from Madhupur National Park at Madhupur Upazila in the Mymensingh district, Bangladesh. The Aspergillus niger was isolated and identified from the soil samples by following conventional mycological methods (cultural and morphological characteristics), followed by confirmatory identification by a polymerase chain reaction (PCR) of conserved sequences of ITS1 ribosomal DNA using specific oligonucleotide primers. This was followed by genus- and species-specific primers targeting Aspergillus niger with an amplicon size of 521 and 310 bp, respectively. For pigment production, a mass culture of Aspergillus niger was conducted in Sabouraud dextrose broth in shaking conditions for seven days. The biomass was subjected to extraction of the pigments following an ethanol-based extraction method and concentrated using a rotary evaporator. Aspergillus niger could be isolated from three samples. The yield of extracted brown pigment from Aspergillus niger was 0.75% (w/v). Spectroscopic analysis of the pigments was carried out using a UV–VIS spectrophotometer. An in vivo experiment was conducted with mice to assess the toxicity of the pigments. From the colorimetric and sensory evaluations, pigment-supplemented products (cookies and lemon juice) were found to be more acceptable than the control products. This could be the first attempt to use Aspergillus niger extracted pigment from soil samples in food products in Bangladesh, but for successful food production, the food colorants must be approved by a responsible authority, e.g., the FDA or the BSTI. Moreover, fungal pigments could be used in the emerging fields of the food and textile industries in Bangladesh.

Journal of Animal Science and Biotechnology, 2021

Dietary fiber (DF) was considered an antinutritional factor due to its adverse effects on feed in... more Dietary fiber (DF) was considered an antinutritional factor due to its adverse effects on feed intake and nutrient digestibility. However, with increasing evidence, scientists have found that DF has enormous impacts on the gastrointestinal tract (GIT) development, digestive physiology, including nutrient digestion, fermentation, and absorption processes of poultry. It may help maintain the small and large intestine's integrity by strengthening mucosal structure and functions and increasing the population and diversity of commensal bacteria in the GIT. Increasing DF content benefits digestive physiology by stimulating GIT development and enzyme production. And the inclusion of fiber at a moderate level in diets also alters poultry growth performance. It improves gut health by modulating beneficial microbiota in the large intestine and enhancing immune functions. However, determining the source, type, form, and level of DF inclusion is of utmost importance to achieve the above-noted benefits. This paper critically reviews the available information on dietary fibers used in poultry and their effects on nutrient utilization, GIT development, gut health, and poultry performance. Understanding these functions will help develop nutrition programs using proper DF at an appropriate inclusion level that will ultimately lead to enhanced DF utilization, overall health, and improved poultry growth performance. Thus, this review will help researchers and industry identify the sources, type, form, and amount of DF to be used in poultry nutrition for healthy, cost-effective, and eco-friendly poultry production.

Journal of Istanbul Veterinary Sciences, 2021

This study was carried out to evaluate the efficacy of Fenugreek seeds (Trigonella foenum-graecum... more This study was carried out to evaluate the efficacy of Fenugreek seeds (Trigonella foenum-graecum L.) on overall performance of broiler. A total of 96-day old Cobb-500 chicks were randomly divided into four dietary treatment groups namely T0, T1, T2and T3having three replications in each treatment group. Brooded chicks were randomly separated into replications wise separate pen to rear up to 4 weeks. Each treatment group contains 24 birds (8 birds in each replication). Experimental birds in T1, T2 and T3 were provided fenugreek seeds meal with 0.5%, 1% and 1.5% of feed while T0was provided with standard feed and considered as control group. The results of this study were indicated that final live weight gain and feed efficiency of birds was significantly (P<0.05) higher in T3 compared to T2, and T0 respectively. The result also indicated that feed efficiency was increased at dose rate of 1.5% fenugreek seeds meal in T3 compared to T2, T1 and control T0 group respectively. In case of meat yield parameters there was significant (P<0.05) difference among treatment groups except liver weight. The carcass weight was significantly (P<0.05) higher in T3 group compared to the control group. The lowest feed cost was found in T0 and highest profit in T3group. Based on the current study, it is concluded that fenugreek seed meal at a dose of 1.5% can be used as growth promoter for the production of broiler chicken.

Veterinary Sciences: Research and Reviews, 2021

Administration of peptide drugs like insulin through the oral avenue specially the gastro-intesti... more Administration of peptide drugs like insulin through the oral avenue specially the gastro-intestinal tract represents the great challenges. Polymeric nanoparticles are suitable colloidal carriers for insulin delivery. The current study was carried out to evaluate nanoparticle tactics for the oral delivery of insulin in the alloxan-induced hyperglycemia in rat model. Twenty male rats of age four months were randomly allocated into four different groups (T 0 , T 1 , T 2 , and T 3). After acclimatization diabetes was induced in three groups of rats (T 1 ; positive control, T 2 : insulin only insulin, and T 3 ; insulin with nano particles) by administered alloxan injection at dose of 120mg/kg body weight intraperitoneally. Group T 0 was kept for negative control while T 1 was kept positive control. T 2 were considered as insulin treated group at 21IU/kg and T 3 as insulin with nano particles of same dose as insulin. Over the sequence of the experimental trials, observations were noted for blood glucose level, glycosylated hemoglobin, and body weight. Blood glucose levels were increased significantly (P<0.05) in all treated groups compared to the negative control group. Similarly, bodyweight was decreased significantly (P<0.05) in all alloxan treated groups compared to negative control. There was significant falling in blood glucose level in insulin with nanoparticle treated group (T 3) compared to only insulin treated group (T 2) and (T 1) that was received alloxan. The present study shows that insulin administration of oral route with nanoparticles was significantly decrease blood glucose levels and increase body weight. Hence, it can be concluded that oral insulin supplementation with nano particles may have significant effect on hyperglycemic controls.

Veterinary Sciences: Research and Reviews, 2021

A cross-sectional study using random sampling was conducted from May to September 2020 in Benadir... more A cross-sectional study using random sampling was conducted from May to September 2020 in Benadir region of Somalia, to determine the knowledge, attitude, and practices of population towards Brucellosis. In this study, a total of 120 participants share their knowledge, attitude, and practices towards Brucellosis. Their knowledge regarding causative agent was 37.5% (n=45) and the disease was 45% (n=54), while transmission of animal to human was 43.33% (n=52). The majority of participants would take actions to ensure the animal is healthy when they buy 95% (n=114), all participants would like to receive more information about the disease and majority of participants have good practices when they handling the domesticated animals and their secretions 96.66% (n=116). Populations who are connected with livestock management or eating livestock products are frequently aware of the disease brucellosis. Though, they do not have as considerable knowledge, what is required to help in control it. Veterinarians, health care workers and respective government bodies must take action to increase the level of awareness and considerate by providing programs or any other technique that can be helpful.

EC Veterinary Science, 2020

A retrospective study was designed to investigate the prevalence of poultry diseases in southern ... more A retrospective study was designed to investigate the prevalence of poultry diseases in southern Somalia (Mogadishu, Baidoa and Kismayo Cities) from January 2018 to August 2020. A total of 4042 diseased cases were recorded, among them the prevalence of viral diseases was noticed higher in all three Cities (Mogadishu City: 37.36%, Baidoa City: 39.53% and Kismayo City: 46%) followed by bacterial disease, parasitic disease and non-infectious disease (Mogadishu city: 30.64%, 17.25%, 14.42%, Baidoa City: 32.11%, 14.84%, 12.19% and Kismayo City: 30.39%, 14.93%, 10.28% respectively). As far we know, no such report was published yet so we conduct this study which may help in developing control strategies against poultry diseases and formulating the policy at government level in future.

Animals, 2020

Probiotics are live microorganisms which, when administered in adequate amounts, confer health be... more Probiotics are live microorganisms which, when administered in adequate amounts, confer health benefits to the host. The use of probiotics in poultry has increased steadily over the years due to higher demand for antibiotic-free poultry. The objective of this systematic review is to present and evaluate the effects of probiotics on the nutrient utilization, growth and laying performance, gut histomorphology, immunity, and gut microbiota of poultry. An electronic search was conducted using relevant keywords to include papers pertinent to the topic. Seventeen commonly used probiotic species were critically assessed for their roles in the performance and gut health of poultry under existing commercial production conditions. The results showed that probiotic supplementation could have the following effects: (1) modification of the intestinal microbiota, (2) stimulation of the immune system, (3) reduction in inflammatory reactions, (4) prevention of pathogen colonization, (5) enhancement of growth performance, (6) alteration of the ileal digestibility and total tract apparent digestibility coefficient, and (7) decrease in ammonia and urea excretion. Thus, probiotics can serve as a potential alternative to antibiotic growth promoters in poultry production. However, factors such as the intestinal health condition of birds, the probiotic inclusion level; and the incubation conditions, feedstuff, and water quality offered to birds may affect the outcome. This systematic review provides a summary of the use of probiotics in poultry production, as well as the potential role of probiotics in the nutrient utilization, growth and laying performance, and gut health of poultry.

Veterinary Sciences: Research and Reviews, 2020

A cross-sectional study of camel mastitis was conducted on 150 lactating camels from July to Dece... more A cross-sectional study of camel mastitis was conducted on 150 lactating camels from July to December 2019 to figure out the prevalence of both clinical and subclinical mastitis in lactating camels in the Benadir Region of Somalia and also to recognize the associated microorganisms as causal agents of mastitis. The
prevalence of this study was measured by using California mastitis test (CMT). Milk samples were collected from the Deyniile District, Benadir Region of Somalia. The overall prevalence of mastitis was 16.66% (7.93% on the quarter basis), the prevalence of clinical and subclinical mastitis was found to be 22.78% and 9.85% on
the animal basis and 9.37% and 6.15% on quarter basis, respectively. The hind quarters were more frequently affected than the fore-quarters. The bacteriological inspection of milk samples revealed that Staphylococcus spp. was the primary etiological agent in both clinical and subclinical mastitis in camels (38.88%), followed by Streptococcus spp. (18.51%), Enterobacterium spp. (14.81%), Corynebacterium spp. (14.81%), Micrococcus spp. (5.55%), Pasteurella spp. (5.55%) and Pseudomonas aeruginosa (1.85%). The prevalence of camel mastitis in the study area was significantly low this might be because of the environmental factors as well as the hygienic managements of the farms. Therefore, implementation of integrated approaches has great importance in the study sites for the avoidance and management of mastitis hence minimizing economic loss and prevents significant public health risks.

Clinical Case Reports, 2020

With timely recognition and surgical intervention along with administration of an antibiotic, ant... more With timely recognition and surgical intervention along with administration of an antibiotic, antihistaminic, and anti‐inflammatory drugs, evisceration of umbilical content with extensive adhesion can be treated, and the outcome is also good.

Veterinary Sciences: Research and Reviews, 2020

The resistance of antibiotics to organisms is a matter of global concern. Methicillin-resistant S... more The resistance of antibiotics to organisms is a matter of global concern. Methicillin-resistant Staphylococcus aureus (MRSA) infection is rapidly increasing in both humans and animals. A two and half years old indigenous calf brought with myiasis wound and bacteria associated with myiasis wound was studied and further molecular detection confirms the presence of methicillin-resistant Staphylococcus aureus from the outer part of the wound but not from the inner part. The study helps and aware the veterinarians, health-workers, and general people regarding the situation of antibiotic resistance. As maggot helps in the reduction of bacteria, this can be used as medical therapy in the case of a different wound.

This report documents the work performed and completed during the internship under the supervisio... more This report documents the work performed and completed during the internship under the supervision of Dr. Woo Kyun Kim of the Department of Poultry Science, College of Agriculture and Environmental Science, University of Georgia, Athens, USA. This report will give an overview of the works performed during the period of internship in Dr. Kim’s lab, feed mill, and poultry research center with technical details. The obtained results will be analyzed and discussed.

Journal of Istanbul Veterınary Scıences, 2020

The study mentioned here was designed to investigate both bacterial pathogens and risk factors as... more The study mentioned here was designed to investigate both bacterial pathogens and risk factors associated with the occurrence of naval ill in calves. A total of 350 calves diagnosed to have navel ill formed the population in our study. Incidence density reports representing the period between 2009 and 2018 were obtained from Veterinary Teaching Hospital (VTH), Bangladesh Agricultural University where information about age, sex, breed, seasonal effect was also included. Umbilical swab from each individual suffering from confirmed navel ill was collected for microbiological study. The occurrence of navel ill in the age of <30 days (n = 244, 69.71%) was noted higher compared to the age of ≥30 days (n = 106, 30.29%). Male calves were highly susceptible (n = 192, 54.86%) compared to females (n=158, 45.14%). In cross breed individuals, the occurrence was higher (n = 330, 94.29%) in regard of indigenous calves (n=20, 5.71%). The occurrence of the illness in summer (March-June) was more common (n = 159, 45.43%) in comparison to both rainy (n = 111, 31.71%) and winter (n = 80, 22.86%) seasons. In term of microbiological study, Staphylococcus aureus, E. coli and Proteus spp. were identified and isolated from the infected areas of the calves with the disease. In conclusion, navel ill occurred more commonly in male calves with the age of less than one month. The appearance of navel ill was noted to be more frequently in summer season (March-June) in cross breed calves. The results also demonstrated that the condition is mediated by mixed bacterial infection formed by gram-positive and gram-negative agents which induce the initial inflammation.

Iranian Journal of Veterinary Surgery, 2020

Case Description-Umbilical hernia is occasionally seen with different levels of complexity in ani... more Case Description-Umbilical hernia is occasionally seen with different levels of complexity in animals This emergency condition requires quick diagnosis and is corrected with various surgical approaches. The aim of this paper is to report the surgical correction of an eviscerated umbilical hernia with extensive adhesion in a bovine calf. Twenty days old indigenous male bovine calf was presented for surgical treatment of an umbilical hernia with extensive adhesion and evisceration. Clinical Findings-Physical examination through palpation revealed evisceration of umbilical content with extensive adhesion. Treatment and Outcome-The surgical procedure was carried out aseptically following standard procedure. The bovine calf recovered without any complication two weeks after surgery. Clinical Relevance-It can be concluded that surgical management along with administration of antibiotic, antihistaminic and anti-inflammatory drugs are effective for successful management of eviscerated umbilical hernia with extensive adhesion in the bovine calf.

Turkish Journal of Veterinary Research, Sep 30, 2019

Objectives: The study was focused to investigate the occurrence of a fatal capture myopathy (CM) ... more Objectives: The study was focused to investigate the occurrence of a fatal capture myopathy (CM) after chemical immobilization during translocation in different places and to evaluate serum enzymes of stressed deer and pathologic alteration of vital organs of dead animals due to CM.. Immediate after capture the animals were allowed to normalize body temperature by pouring sufficient water over the body. Peripheral blood was aspirated from jugular vein for serum biochemical analysis. Once the animals died, vital organs were collected and processed for histology. Results and Discussion: Data from 2013 to 2018 revealed 178 animals captured through darting and among them 40 animals died due to post-capture myopathy reflecting death rate of 22.47%. We have closely studied on 16 animals captured on different occasions. Serum enzyme analysis were exhibited increased levels of ALT, AST, Bilirubin, Creatinine, BUN, LDH, CK, Troponin, Cholesterol, Triglyceride, HDL and LDL and were highly indicative of stress-linked muscle and organ damage. The macroscopic lesions consisted of muscular and cardiac degeneration, edema, hemorrhage and congestion in lung, adrenal gland and in kidney. Microscopically there were loss of striation and fragmentation of skeletal muscle, formation of contraction band necrosis in myocardial fiber, degenerative changes in renal tubule and formation of central intraluminal eosinophilic casts. Conclusions: The pathological findings were indicative of capture myopathy in spotted deer. This report underlines that mortality from capture is a risk that must be considered during restocking programs.

Bangladesh Veterinary Journal , 2018

A retrospective study was conducted to investigate the occurrence of Schistosomus reflexus in cal... more A retrospective study was conducted to investigate the occurrence of Schistosomus reflexus in calves from January 2012 to December 2016. Occurrence of Schistosomus reflexus in neonatal calves was studied in relation to breed, sex and seasons of calving. Clinical signs were angulation of limbs and no abdominal closure. Results showed 8.41% Schistosomus reflexus in surgically intervened cows. The percentage of indigenous calves affected with Schistosomus reflexus was as high as 42.86%, and in crossbred this was 57.14%. The percent of female calves born with Schistosomus reflexus was higher (60.0%) than the males (40.0%). Maximum occurrence (42.86%) of Schistosomus reflexus was recorded in the summer followed by rainy (37.14%) and winter seasons (20.0%). Among the studied areas, highest occurrence was recorded in Mymensingh Sadar (68.57%) followed by in Patuakhali Sadar (17.14%) and Bauphal (14.29%) upazilla of Patuakhali district. However, this study provides some basic information to veterinarians, researchers and farmers about the occurrence of Schistosomus reflexus in calves.

Research in Agriculture Livestock and Fisheries, 2018

The study has been directed to find out the occurrence of surgical affections in herbivorous zoo ... more The study has been directed to find out the occurrence of surgical affections in herbivorous zoo animals in National Zoological Garden, Dhaka, Bangladesh predicated on record books from 2012 to 2016. A total of 330 surgically affected animals were recorded. The overall occurrence of surgical affections was higher in Bovidae (34.85%) followed by Cervidae (25.45%), Equidae (13.94%), Cercopithecidae (10.61%), Giraffidae (4.85%), Elephantidae (4.55%) Hippopotamidae (4%), Rhinocerotidae (1.52%) and Macropodidae (0.61%). In this study, it is found a higher occurrence of surgically affected are male than female animals. The overall occurrence in male was 56.36% and those in female, it was 43.64%. In terms of different affections, the highest occurrence was wound (13.03% male, 16.67% female) and followed by lameness (14.55% in male, 11.82% in female), skin lesions (9.39% in male, 6.67% in female), myiasis (5.76% in male, 3.33% in female), Horn fracture 3.33% (Only in male), arthritis (2.42% male, 1.52% female), hoof injury (1.82% male, 1% female), abscess (1.52% male, 1.21% female), sore (0.61% male, 2.12% female), Fracture (1.21% male, 0.3% female), Paralysis (0.61% male, 0.3% female) and Corneal opacity (0.61% male, 0.3% female). This report may help to develop control strategies against major surgical affections reported in this study.

Research in Agriculture Livestock and Fisheries, 2018

This study was carried out to investigate the occurrence of various surgical affections often enc... more This study was carried out to investigate the occurrence of various surgical affections
often encountered in carnivorous zoo animals of National Zoological Garden, Dhaka,
Bangladesh. The study was based on the surgical cases reported in the day record
book maintained by the zoo authority during the period of January - December 2012 to
January - December 2016. Of 74 surgically affected zoo carnivore’s cases; Felididae
recorded highest 81.08 %, Canidae 12.61 % and, Ursidae 6.76 %. When affections
were compared between sexes, it was observed higher in male 62.16 % than in female
37.84 %. Among the different surgical affections; the wound cases were observed at
the apex with 29.7 % and 18.9 %, followed by paralysis 10.08 % and 6.8 %, skin injury
9.5 % and 6.8 %, myiasis 4.1 % and 2.7 %, lameness 2.7 % and 4.1 % in male and
female, respectively. While, tail sore 1.4 %, conjunctivitis 1.4 % and hoof injury 1.4 %
were observed in male carnivores only. In conclusion, this report signifies the common
surgical affections suffered by zoo carnivores, while male carnivores showed
comparatively more prone to affections than female one. This report may be helpful to
develop a control strategy in managing zoo animal captivity and further minimizing
these surgical affections.

Journal of Bangladesh Agricultural University, 2018

This study was performed to investigate the recurrence of the umbilical hernia in bovine calves a... more This study was performed to investigate the recurrence of the umbilical hernia in bovine calves at Veterinary Teaching Hospital (VTH), Bangladesh Agricultural University, Mymensingh. A total of 34 animals were admitted to VTH diagnosed to have the umbilical hernia, among them 13 were recurrent cases. The diagnosis of umbilical hernia was based on the disease history, clinical signs, palpation, needle
exploration, and auscultation. The risk factors like age, sex, breed, body weight, the season of affection, size of the hernial ring, suture materials, error in suture patterns and involvement of infections in the recurrence were considered. Infected samples were collected from recurrent patients with infected umbilicus region, and subjected to bacteriological analysis. We found the recurrence percentage was higher in the crossbred (44%) than that in the indigenous (22.22%) calves. It was also observed that
recurrence percentage was higher in male (42.11%) than those in female (33.33%) patients. The recurrence percentage was also higher in calves aged below three months (40.74%) than the calves of above three months (28.57%). It was found that recurrence percentage was most frequent in summer (46.15%) and the least in the winter (30%). Among 13 recurrent bovine calves after herniorrhaphy, three calves returned to VTH with umbilical abscess, six calves recurred due to non-infectious causes like failure of suture materials, errors in suture pattern or management fault and 4 calves recurred with both umbilical abscess and non-infectious causes. From this study, it may be concluded that synthetic absorbable suture materials with secured suture pattern and good postoperative management may help in reducing the recurrence of an umbilical hernia.

Conference Presentations by Pravin Mishra

Proceeding of the 15th ISCon. 2018 of CVASU, 2018

Fungal infections in chickens during brooding condition as well as in adult condition are common ... more Fungal infections in chickens during brooding condition as well as in adult condition are common in Bangladesh. However, very few reports have been reported in Bangladesh focusing on isolation and molecular identification of the pathogenic fungi from chickens. The objective of this study was to isolate and identify the pathogenic fungi from chickens followed by their confirmatory diagnosis by PCR. A total of 50 suspected dead chickens comprising of broilers (n=32) and layers (n=18) aging between 5 days and 45 weeks were collected during January to June 2016 from poultry farms located at Gazipur, Bangladesh. Lung samples from the suspected dead chicken were collected. Isolation of the associated toxigenic fungi was done using Potato Dextrose Agar (PDA). The fungi were primarily identified based on the colony morphology. DNA was extracted from the suspected colonies, and was used for the detection of Aspegilli using genus specific primers (ASAP-1 and ASAP-2). The Aspergilli were then screened by PCR using species specific primers targeting A. flavus (FLA-1 and FLA-2), A. fumigatus (ASPU and Af3r) and A. niger (ASPU and Nilr). The overall prevalence of Aspergillus spp. was 44% (n=22/50; p<0.05). Among the Aspergilli, A. flavus was detected in 10% (n=5/50) samples. Similarly, A. fumigatus and A. niger was detected as 26% (n=13/50) and 8% (n=4/50), respectively. Three samples were associated with more than one fungi; two fungi (A. flavus and A. niger) were found in two samples and three fungi (A. flavus, A. fumigatus and A. niger) were found in one sample. It is concluded that the fungal infection caused by A. flavus, A. fumigatus and A. niger are present in the chickens of Gazipur, Bangladesh. Improvement of hygienic measure in the poultry farms in Gazipur is suggested.

Encyclopedia, 2021

Foods, 2021

Journal of Animal Science and Biotechnology, 2021

Journal of Istanbul Veterinary Sciences, 2021

Veterinary Sciences: Research and Reviews, 2021

EC Veterinary Science, 2020

Animals, 2020

Veterinary Sciences: Research and Reviews, 2020

Clinical Case Reports, 2020

Veterinary Sciences: Research and Reviews, 2020

Journal of Istanbul Veterınary Scıences, 2020

Iranian Journal of Veterinary Surgery, 2020

Turkish Journal of Veterinary Research, Sep 30, 2019

Bangladesh Veterinary Journal , 2018

Research in Agriculture Livestock and Fisheries, 2018

Journal of Bangladesh Agricultural University, 2018

Proceeding of the 15th ISCon. 2018 of CVASU, 2018

Proceeding of the 16th ISCon. 2019 of CVASU, 2019

Cryptosporidium is an apicomplexan parasite that causes diarrhea in humans and a wide range of do... more Cryptosporidium is an apicomplexan parasite that causes diarrhea in humans and a wide range of domestic and wild animals in both developing and developed countries. Survey of Cryptosporidium genotypes from animals and humans living in the same region are rare, our understanding of the importance of zoonotic transmission in the epidemiology of cryptosporidiosis remains unclear. Molecular biology has provided powerful new tools for characterizing Cryptosporidium and has revealed considerable variation within the genus. PCR was used to amplify a portion of the Cryptosporidium spp. from fecal DNA of humans and cattle living in Mymensingh, Bangladesh. PCR-positive samples were further analyzed on the basis of 18S rRNA gene, HSP 70, gp60 and MSB. From the analysis, three Cryptosporidium spp. (Cryptosporidium hominis, Cryptosporidium parvum, and Cryptosporidium bovis) were recognized, and zoonotically transmitted Cryptosporidium parvum was the predominant species found in humans. Out of 41 samples, 19.5% 18S rRNA 17% HSP-70, 7.31% gp60 and 14.63% MSB positive Cryptosporidium were detected. Sequencing of the 18S rRNA amplicons revealed the presence of 75% Cryptosporidium hominis, 12.5% Cryptosporidium parvum in human and 12.5% Cryptosporidium bovis in calf. Sequencing of HSP-70 amplicons reveals the presence of 14.28% Cryptosporidium parvum in human, 14.12% Cryptosporidium bovis in calf and 71.42% Cryptosporidium hominis both in human and calf. Sequencing of MSB and gp60 amplicons confirms the presence of Cryptosporidium hominis (100%) and Cryptosporidium hominis subtype Ia (100%) respectively in humans. Presence of C. hominis in calf indicates the potential of this subtypes Ia to transmit to other animals like calf.