Vanja Tadic - Academia.edu (original) (raw)

Papers by Vanja Tadic

Molecules

A comparative study on essential oils extracted from Mentha suaveolens Ehrh. from Italy is report... more A comparative study on essential oils extracted from Mentha suaveolens Ehrh. from Italy is reported. Two extraction procedures were investigated: hydrodistillation and steam distillation, carried out as a continuous and fractionated procedure. Fresh and dried plant material from two harvests was used. The hydrodistillation method yielded a higher amount of essential oil. The dried plant was significantly richer in essential oil per kg of starting plant material. Gas chromatography-mass spectrometry analysis of 112 samples showed that the essential oils belong to the piperitenone oxide-rich chemotype. In addition, piperitenone, p-cymen-8-ol, and limonene were among the most abundant compounds in the different samples. A higher amount of piperitenone oxide was obtained by hydrodistillation, while steam distillation gave a higher percentage of piperitenone and limonene. The essential oils were characterized for their anti-Candida albicans activity; higher potency was observed for the s...

Makedonsko farmacevtski bilten, Dec 31, 2022

Savremene tehnologije, 2013

Elecampane (Inula helenium L., Asteraceae), a well-known traditional medicinal herb, and its root... more Elecampane (Inula helenium L., Asteraceae), a well-known traditional medicinal herb, and its root (Inulae radix) have been used as antiseptics, expectorants, diuretics and bactericidal agents. The aim of this study was to investigate the antimicrobial activity of different Inulae radix isolates against microorganisms that commonly cause skin infections. The essential oil obtained by hydrodistillation (IH-HD) and Sohxlet extracts, ether (IH-SOX-E) and ethanol (IH-SOX-Et) fractions were investigate The isolates were dissolved in DMSO at the concentration of 2560 to 0.625 μg/mL. The antimicrobial activity was tested by using the microdilution method against reference bacterial strains and clinical isolates from skin swabs. IH-SOX-E exhibited a strong antimicrobial activity against all tested strains, IH-HD as well, with the exception of S. aureus, ATCC 25923 (moderate activity), while IH-SOX-Et showed a strong activity only against clinical isolate of C. albicans. The results of our study indicate that elecampane root isolates may be used for incorporation into phytopreparations for topical application in treating skin infections, especially those caused by C. Albicans the most possible sites for interactions with other compounds.

Lekovite sirovine, 2018

Lichens represent a promising source of antimicrobial, cytotoxic and antioxidant agents. Their gr... more Lichens represent a promising source of antimicrobial, cytotoxic and antioxidant agents. Their great pharmacological potential lies in the fact that they represent specific symbiotic organisms and thus possess natural roles allowing them to be highly adaptable to different environmental conditions. On the other hand, stated biological activities of lichens with prospective medicinal significance may be connected to their long-term use in the traditional treatment of various ailments. Genus Usnea from the Parmeliaceae family is certainly one of the best studied in terms of chemical composition and biological properties of its extracts and/or isolated compounds. In the first part of the study, a detailed review of the literature has been performed yielding a detailed report on the investigations of biological activities of the lichens belonging to this genus. In the second part of the study, the chemical composition of the lichens from the genus was described and, additionally, a survey of the biological properties of the most representative secondary metabolites in these lichens has been reported. It could be concluded that the extracts and/or isolated compounds from the lichens belonging to the genus Usnea may be considered a valuable source of prospective drug candidates with potential clinical relevance.

Acta Poloniae Pharmaceutica, Jun 28, 2019

In the present work, fruits from two plant species, Rosa canina L. and Sorbus aucuparia L., popul... more In the present work, fruits from two plant species, Rosa canina L. and Sorbus aucuparia L., popular in traditional folk medicine in Serbia, were studied. The aim was to examine and compare the efficiency of the ultrasonic extraction with different solvents regarding physicochemical properties, polyphenolic profile of extracts, as well as their tyrosinase inhibitory and antioxidant activity. The polyphenols evaluation indicated that water was the best solvent for a thorough extraction of bioactive compounds from the R. canina fruits, while propylene glycol-water (45 : 55, v/v) was the most efficient regarding S. aucuparia fruits, followed by ethanol-water (7 : 3, v/v). Only flavonoids were more abundant in S. aucuparia fruit extracts. R. canina water extracts showed higher antioxidant activity, using several in vitro tests with different working principles. However, S. aucuparia ultrasonic extracts with propylene glycol-water (45 : 55, v/v) demonstrated a higher potential concerning tyrosinase inhibitory and chelating activity. Therefore, these ultrasonic extracts, being great sources of natural anti-tyrosinase inhibitors and antioxidants, can be considered as promising candidates suitable for pharmaceutical application, as great sources of natural anti-tyrosinase inhibitors and antioxidants.

International Journal of Cosmetic Science, Jun 1, 2019

The aim of the study was in vitro and in vivo characterization of cosmetic cream with 6% of stand... more The aim of the study was in vitro and in vivo characterization of cosmetic cream with 6% of standardized wild apple fruit extract, stabilized by conventional non‐ionic emulsifier‐CEW, in order to determine the influence of emulsifiers (conventional vs. biodegradable) on the characteristics of creams and their effects on the skin.

Antioxidants, Mar 16, 2021

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Makedonsko farmacevtski bilten, Dec 31, 2022

Natural Product Research, Feb 6, 2023

In the present study, we investigated the effect of supercritical carbon-oxide (scCO2) extraction... more In the present study, we investigated the effect of supercritical carbon-oxide (scCO2) extraction on antioxidant capacity, enzyme inhibitory potential, and levels of ergosterol and ganoderic acid in both cultivated and wild-grown G. lucidum. Extraction yields were slightly higher for wild samples (1.29%) than for cultivated ones (1.13%). The levels of ganoderic acid and ergosterol were higher in cultivated in comparison to wild samples. In addition, the total phenolic content in cultivated samples (13.42 mg GAE g-1) was higher than in wild samples (10.38 mg GAE g-1). In general, cultivated samples exhibited stronger antioxidant potential when compared with wild ones. Regarding enzyme inhibitory properties, it was validated that the wild samples (14.01 mg OE g-1) possessed greater lipase activity in comparison to cultivated samples (5.36 mg OE g-1). Based on our findings, cultivated G. lucidum might be considered a valuable source of natural bioactive agents in the preparation of health-promoting products.

Journal of the Mexican Chemical Society, 2021

This work aimed to obtain a validated model for the prediction of retention times of compounds is... more This work aimed to obtain a validated model for the prediction of retention times of compounds isolated from Origanum heracleoticum, Origanum vulgare, Thymus vulgaris, and Thymus serpyllum essential oils. In total 68 experimentally obtained retention times of compounds, which were separated and detected by GC-MS were further used to build the prediction models. The quantitative structure-retention relationship was employed to foresee the Kovats retention indices of compounds acquired by GC-MS analysis, using eight molecular descriptors selected by a genetic algorithm. The chosen descriptors were used as inputs for the four artificial neural networks, to construct a Kovats retention indices predictive quantitative structure-retention relationship model. The coefficients of determination in the training cycle were 0.830; 0.852; 0.922 and 0.815 (for compounds found in O. heracleoticum, O. vulgare, T. vulgaris and T. serpyllum essential oils, respectively), demonstrating that these models could be used for prediction of Kovats retention indices, due to low prediction error and high r 2 .

PONS - medicinski casopis, 2019

Objective. The literature provides data on the antioxidant activity of different botanical parts ... more Objective. The literature provides data on the antioxidant activity of different botanical parts of walnut (Juglans regia L., Juglandaceae) growing in various countries. This research has evaluated the antioxidant activity of pericarp (green husk) of unripe walnut fruit, originated from Sumadija region in Serbia. Methods. The methanol extract of walnut husk was characterized by the total phenol and tannin content, while antioxidant evaluation was done by DPPH radical scavenging assay. Results. It has been found that the antioxidant activity of walnut pericarp is within the range 25.32-79.35 % and is concentration dependent. The IC50 is 1.223 mg/ml. The total phenol content was 119.8 mg GAE/g and tannin 2.645 %. Conclusion. The investigated parameters of the green walnut pericarp from Sumadija region fit the range of values documented for different parts of walnut species growing in other areas in the world.

The Journal of Supercritical Fluids, 2018

Highlights  SSI enabled incorporation of high amounts of pyrethrum extract in tested materials ... more Highlights  SSI enabled incorporation of high amounts of pyrethrum extract in tested materials  The largest loading capacity among the tested materials had PP fabric  Cellulose acetate is a promising carrier for controlled release of pyrethrum extract  Physical form of cellulose acetate influenced the rates of impregnation and release  Amount of pyrethrum extract in polyamide fabric remained stable after 30 days

RAD Conference Proceedings, 2017

Solar radiation has harmful effects on exposed skin, producing accelerated aging processes (wrink... more Solar radiation has harmful effects on exposed skin, producing accelerated aging processes (wrinkles, dryness, telangiectasia, dyspigmentations). Also, there is an increased ROS generation in skin exposed to the UV-A and UV-B radiation. This results in oxidative stress, photodamage of skin macromolecules and photocarcinogenesis processes. In order to prevent this, botanical extracts with antioxidant properties can be used in anti-photoaging preparations, as a substitute for traditional sunscreen products. Plant extracts, rich in natural polyphenols, exert fewer sensitization effects on skin and are very effective against oxidative damaging caused by UV radiation. The aim of this study was to evaluate the antioxidative activity of different elder (Sambucus nigra L.) fruit extracts. Active components of S. nigra, such as polyphenols, have an important biological activity. Fruit extracts were obtained by the maceration method using four different solvents (methanol-ME, propylene glycol 45% v/v-PE, ethanol 70% v/v-EE and distilled water-WE). To study antioxidant activity we used different in vitro assays: 2,2-diphenyl-1picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP) assay and β-carotene bleaching assay. The concentrations at which 50% of the DPPH radicals were scavenged (IC50) were 3.54, 3.94 and 12.07 mg/ml for the samples EE, ME and PE, respectively. The sample WE showed a stronger scavenging activity (IC50 value was 2.62 mg/ml). FRAP values were 242.29 and 686.43 μmol Fe 2+ /g of dry extract for PE and ME samples, respectively. Higher values were obtained using the EE and WE samples, 793.54 and 934.81 μmol Fe 2+ /g of dry extract, respectively. Sample EE was the most active in the β-carotene bleaching assay (IC50 was 0.235±0.004 mg/ml). Extracts WE and PE showed similar, but lower percentage of inhibition of β-carotene bleaching. FRAP assay showed a significant (p < 0.05) negative correlation (r =-0.975) with radical scavenging capacity (IC50 values). The extract WE (distilled water was used as a solvent) exhibited the highest radical scavenging activity and had the highest ferric reducing potential.

Journal of ethnopharmacology, Jan 2, 2017

Olive or sunflower oil yarrow extracts have been extensively used against inflammatory disorders ... more Olive or sunflower oil yarrow extracts have been extensively used against inflammatory disorders and skin wound healing in traditional medicine. To evaluate oil yarrow extracts traditional use in treatment of topical/dermatological skin impairments, sodium lauryl sulfate test was applied, and in vivo measuring of the biophysical parameters (erythema index, skin capacitance and the pH of the skin) in the artificially irritated skin was performed. As traditionally olive and sunflower oil have been used equally for extracts production, the experiment was carried out to investigate whether any of the oil extractants has the advantage over the other, and if the method of extraction might influence the desired activity. The observed activity has been connected to the chemical profile of the investigated extracts and their antioxidative properties. In vivo measurements were performed using the appropriate probes for measuring skin capacitance, pH of the skin and erythema index (EI). The de...

Journal of Ethnopharmacology, 2019

Ethnopharmacological relevance Alchemilla vulgaris is an important remedy in European folk medici... more Ethnopharmacological relevance Alchemilla vulgaris is an important remedy in European folk medicine, known for its adstringent and anti-inflammatory properties; it is traditionally used to heal gynecological and gastrointestinal diseases. Despite its folkloric use in wound healing, there is a lack of scientific data to support this therapeutic application.

Open Chemistry, 2015

Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (... more Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (SFE), SFE followed by isomerization, as well as by conventional technique, were investigated for their chemical composition and antibacterial activity against selected foodborne pathogens and microorganisms capable to cause the food spoilage. The antibacterial activity of the extracts was compared with the antibacterial activity of xanthohumol, compound known for its broad pharmacological properties, isolated from the raw material remained after the SFE. Xanthohumol (XH, 96%) proved to posses the most prominent activity against all the tested strains, with the MIC values ranged between 2.5 and 20 μg mL-1. Supercritical hops extract and potassium isomerized supercritical hops extract showed strong antibacterial activity against the tested strains as well. Escherichia coli was not affected by the extracts, meaning that their oral admission would not cause the same problem as antibiotic app...

Journal of Ethnopharmacology

Pharmaceutics

The dual controlled release of emulgels makes them efficient drug delivery systems of increasing ... more The dual controlled release of emulgels makes them efficient drug delivery systems of increasing interest. The framework of this study was to incorporate selected L-ascorbic acid derivatives into emulgels. From the formulated emulgels, the release profiles of actives were evaluated considering their different polarities and concentrations, and consequently their effectiveness on the skin via a long-term in vivo study that lasted for 30 days was determined. Skin effects were assessed by measuring the electrical capacitance of the stratum corneum (EC), trans-epidermal water loss (TEWL), melanin index (MI) and skin pH. In addition, the sensory and textural properties of emulgel formulations were compared with each other. The changes in the rate of the release of the L-ascorbic acid derivatives were monitored using the Franz diffusion cells. The obtained data were statistically significant, and indicated an increase in the degree of hydration of the skin and skin whitening potential, wh...

Antioxidants

The aim of this study was to investigate the effect of the use of green solvents, natural deep eu... more The aim of this study was to investigate the effect of the use of green solvents, natural deep eutectic solvents (NaDES), in comparison with conventional solvents on the extraction of free unbound phenolic compounds and the antioxidant activity of extracts of dried bilberry fruit, bilberry leaves and green tea leaves. After preparation of the extracts via ultrasound-assisted extraction using NaDES and conventional solvents (water and ethanol), spectrophotometric determination of total phenolic and flavonoid content, HPLC analysis of extracted polyphenols and antioxidant determination using FRAP, DPPH and ABTS assays were conducted. The results showed that NaDES have a great potential as agents for the extraction of phenolic compounds with potent antioxidant activity; the highest values of phenolic content and antioxidant activity were detected in the samples obtained by extraction using the NaDES combination betaine + urea. The bilberry leaves exhibited the highest flavonoid content...

Journal of CO2 Utilization

Molecules

A comparative study on essential oils extracted from Mentha suaveolens Ehrh. from Italy is report... more A comparative study on essential oils extracted from Mentha suaveolens Ehrh. from Italy is reported. Two extraction procedures were investigated: hydrodistillation and steam distillation, carried out as a continuous and fractionated procedure. Fresh and dried plant material from two harvests was used. The hydrodistillation method yielded a higher amount of essential oil. The dried plant was significantly richer in essential oil per kg of starting plant material. Gas chromatography-mass spectrometry analysis of 112 samples showed that the essential oils belong to the piperitenone oxide-rich chemotype. In addition, piperitenone, p-cymen-8-ol, and limonene were among the most abundant compounds in the different samples. A higher amount of piperitenone oxide was obtained by hydrodistillation, while steam distillation gave a higher percentage of piperitenone and limonene. The essential oils were characterized for their anti-Candida albicans activity; higher potency was observed for the s...

Makedonsko farmacevtski bilten, Dec 31, 2022

Savremene tehnologije, 2013

Elecampane (Inula helenium L., Asteraceae), a well-known traditional medicinal herb, and its root... more Elecampane (Inula helenium L., Asteraceae), a well-known traditional medicinal herb, and its root (Inulae radix) have been used as antiseptics, expectorants, diuretics and bactericidal agents. The aim of this study was to investigate the antimicrobial activity of different Inulae radix isolates against microorganisms that commonly cause skin infections. The essential oil obtained by hydrodistillation (IH-HD) and Sohxlet extracts, ether (IH-SOX-E) and ethanol (IH-SOX-Et) fractions were investigate The isolates were dissolved in DMSO at the concentration of 2560 to 0.625 μg/mL. The antimicrobial activity was tested by using the microdilution method against reference bacterial strains and clinical isolates from skin swabs. IH-SOX-E exhibited a strong antimicrobial activity against all tested strains, IH-HD as well, with the exception of S. aureus, ATCC 25923 (moderate activity), while IH-SOX-Et showed a strong activity only against clinical isolate of C. albicans. The results of our study indicate that elecampane root isolates may be used for incorporation into phytopreparations for topical application in treating skin infections, especially those caused by C. Albicans the most possible sites for interactions with other compounds.

Lekovite sirovine, 2018

Lichens represent a promising source of antimicrobial, cytotoxic and antioxidant agents. Their gr... more Lichens represent a promising source of antimicrobial, cytotoxic and antioxidant agents. Their great pharmacological potential lies in the fact that they represent specific symbiotic organisms and thus possess natural roles allowing them to be highly adaptable to different environmental conditions. On the other hand, stated biological activities of lichens with prospective medicinal significance may be connected to their long-term use in the traditional treatment of various ailments. Genus Usnea from the Parmeliaceae family is certainly one of the best studied in terms of chemical composition and biological properties of its extracts and/or isolated compounds. In the first part of the study, a detailed review of the literature has been performed yielding a detailed report on the investigations of biological activities of the lichens belonging to this genus. In the second part of the study, the chemical composition of the lichens from the genus was described and, additionally, a survey of the biological properties of the most representative secondary metabolites in these lichens has been reported. It could be concluded that the extracts and/or isolated compounds from the lichens belonging to the genus Usnea may be considered a valuable source of prospective drug candidates with potential clinical relevance.

Acta Poloniae Pharmaceutica, Jun 28, 2019

In the present work, fruits from two plant species, Rosa canina L. and Sorbus aucuparia L., popul... more In the present work, fruits from two plant species, Rosa canina L. and Sorbus aucuparia L., popular in traditional folk medicine in Serbia, were studied. The aim was to examine and compare the efficiency of the ultrasonic extraction with different solvents regarding physicochemical properties, polyphenolic profile of extracts, as well as their tyrosinase inhibitory and antioxidant activity. The polyphenols evaluation indicated that water was the best solvent for a thorough extraction of bioactive compounds from the R. canina fruits, while propylene glycol-water (45 : 55, v/v) was the most efficient regarding S. aucuparia fruits, followed by ethanol-water (7 : 3, v/v). Only flavonoids were more abundant in S. aucuparia fruit extracts. R. canina water extracts showed higher antioxidant activity, using several in vitro tests with different working principles. However, S. aucuparia ultrasonic extracts with propylene glycol-water (45 : 55, v/v) demonstrated a higher potential concerning tyrosinase inhibitory and chelating activity. Therefore, these ultrasonic extracts, being great sources of natural anti-tyrosinase inhibitors and antioxidants, can be considered as promising candidates suitable for pharmaceutical application, as great sources of natural anti-tyrosinase inhibitors and antioxidants.

International Journal of Cosmetic Science, Jun 1, 2019

The aim of the study was in vitro and in vivo characterization of cosmetic cream with 6% of stand... more The aim of the study was in vitro and in vivo characterization of cosmetic cream with 6% of standardized wild apple fruit extract, stabilized by conventional non‐ionic emulsifier‐CEW, in order to determine the influence of emulsifiers (conventional vs. biodegradable) on the characteristics of creams and their effects on the skin.

Antioxidants, Mar 16, 2021

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Makedonsko farmacevtski bilten, Dec 31, 2022

Natural Product Research, Feb 6, 2023

In the present study, we investigated the effect of supercritical carbon-oxide (scCO2) extraction... more In the present study, we investigated the effect of supercritical carbon-oxide (scCO2) extraction on antioxidant capacity, enzyme inhibitory potential, and levels of ergosterol and ganoderic acid in both cultivated and wild-grown G. lucidum. Extraction yields were slightly higher for wild samples (1.29%) than for cultivated ones (1.13%). The levels of ganoderic acid and ergosterol were higher in cultivated in comparison to wild samples. In addition, the total phenolic content in cultivated samples (13.42 mg GAE g-1) was higher than in wild samples (10.38 mg GAE g-1). In general, cultivated samples exhibited stronger antioxidant potential when compared with wild ones. Regarding enzyme inhibitory properties, it was validated that the wild samples (14.01 mg OE g-1) possessed greater lipase activity in comparison to cultivated samples (5.36 mg OE g-1). Based on our findings, cultivated G. lucidum might be considered a valuable source of natural bioactive agents in the preparation of health-promoting products.

Journal of the Mexican Chemical Society, 2021

This work aimed to obtain a validated model for the prediction of retention times of compounds is... more This work aimed to obtain a validated model for the prediction of retention times of compounds isolated from Origanum heracleoticum, Origanum vulgare, Thymus vulgaris, and Thymus serpyllum essential oils. In total 68 experimentally obtained retention times of compounds, which were separated and detected by GC-MS were further used to build the prediction models. The quantitative structure-retention relationship was employed to foresee the Kovats retention indices of compounds acquired by GC-MS analysis, using eight molecular descriptors selected by a genetic algorithm. The chosen descriptors were used as inputs for the four artificial neural networks, to construct a Kovats retention indices predictive quantitative structure-retention relationship model. The coefficients of determination in the training cycle were 0.830; 0.852; 0.922 and 0.815 (for compounds found in O. heracleoticum, O. vulgare, T. vulgaris and T. serpyllum essential oils, respectively), demonstrating that these models could be used for prediction of Kovats retention indices, due to low prediction error and high r 2 .

PONS - medicinski casopis, 2019

Objective. The literature provides data on the antioxidant activity of different botanical parts ... more Objective. The literature provides data on the antioxidant activity of different botanical parts of walnut (Juglans regia L., Juglandaceae) growing in various countries. This research has evaluated the antioxidant activity of pericarp (green husk) of unripe walnut fruit, originated from Sumadija region in Serbia. Methods. The methanol extract of walnut husk was characterized by the total phenol and tannin content, while antioxidant evaluation was done by DPPH radical scavenging assay. Results. It has been found that the antioxidant activity of walnut pericarp is within the range 25.32-79.35 % and is concentration dependent. The IC50 is 1.223 mg/ml. The total phenol content was 119.8 mg GAE/g and tannin 2.645 %. Conclusion. The investigated parameters of the green walnut pericarp from Sumadija region fit the range of values documented for different parts of walnut species growing in other areas in the world.

The Journal of Supercritical Fluids, 2018

Highlights  SSI enabled incorporation of high amounts of pyrethrum extract in tested materials ... more Highlights  SSI enabled incorporation of high amounts of pyrethrum extract in tested materials  The largest loading capacity among the tested materials had PP fabric  Cellulose acetate is a promising carrier for controlled release of pyrethrum extract  Physical form of cellulose acetate influenced the rates of impregnation and release  Amount of pyrethrum extract in polyamide fabric remained stable after 30 days

RAD Conference Proceedings, 2017

Solar radiation has harmful effects on exposed skin, producing accelerated aging processes (wrink... more Solar radiation has harmful effects on exposed skin, producing accelerated aging processes (wrinkles, dryness, telangiectasia, dyspigmentations). Also, there is an increased ROS generation in skin exposed to the UV-A and UV-B radiation. This results in oxidative stress, photodamage of skin macromolecules and photocarcinogenesis processes. In order to prevent this, botanical extracts with antioxidant properties can be used in anti-photoaging preparations, as a substitute for traditional sunscreen products. Plant extracts, rich in natural polyphenols, exert fewer sensitization effects on skin and are very effective against oxidative damaging caused by UV radiation. The aim of this study was to evaluate the antioxidative activity of different elder (Sambucus nigra L.) fruit extracts. Active components of S. nigra, such as polyphenols, have an important biological activity. Fruit extracts were obtained by the maceration method using four different solvents (methanol-ME, propylene glycol 45% v/v-PE, ethanol 70% v/v-EE and distilled water-WE). To study antioxidant activity we used different in vitro assays: 2,2-diphenyl-1picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP) assay and β-carotene bleaching assay. The concentrations at which 50% of the DPPH radicals were scavenged (IC50) were 3.54, 3.94 and 12.07 mg/ml for the samples EE, ME and PE, respectively. The sample WE showed a stronger scavenging activity (IC50 value was 2.62 mg/ml). FRAP values were 242.29 and 686.43 μmol Fe 2+ /g of dry extract for PE and ME samples, respectively. Higher values were obtained using the EE and WE samples, 793.54 and 934.81 μmol Fe 2+ /g of dry extract, respectively. Sample EE was the most active in the β-carotene bleaching assay (IC50 was 0.235±0.004 mg/ml). Extracts WE and PE showed similar, but lower percentage of inhibition of β-carotene bleaching. FRAP assay showed a significant (p < 0.05) negative correlation (r =-0.975) with radical scavenging capacity (IC50 values). The extract WE (distilled water was used as a solvent) exhibited the highest radical scavenging activity and had the highest ferric reducing potential.

Journal of ethnopharmacology, Jan 2, 2017

Olive or sunflower oil yarrow extracts have been extensively used against inflammatory disorders ... more Olive or sunflower oil yarrow extracts have been extensively used against inflammatory disorders and skin wound healing in traditional medicine. To evaluate oil yarrow extracts traditional use in treatment of topical/dermatological skin impairments, sodium lauryl sulfate test was applied, and in vivo measuring of the biophysical parameters (erythema index, skin capacitance and the pH of the skin) in the artificially irritated skin was performed. As traditionally olive and sunflower oil have been used equally for extracts production, the experiment was carried out to investigate whether any of the oil extractants has the advantage over the other, and if the method of extraction might influence the desired activity. The observed activity has been connected to the chemical profile of the investigated extracts and their antioxidative properties. In vivo measurements were performed using the appropriate probes for measuring skin capacitance, pH of the skin and erythema index (EI). The de...

Journal of Ethnopharmacology, 2019

Ethnopharmacological relevance Alchemilla vulgaris is an important remedy in European folk medici... more Ethnopharmacological relevance Alchemilla vulgaris is an important remedy in European folk medicine, known for its adstringent and anti-inflammatory properties; it is traditionally used to heal gynecological and gastrointestinal diseases. Despite its folkloric use in wound healing, there is a lack of scientific data to support this therapeutic application.

Open Chemistry, 2015

Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (... more Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (SFE), SFE followed by isomerization, as well as by conventional technique, were investigated for their chemical composition and antibacterial activity against selected foodborne pathogens and microorganisms capable to cause the food spoilage. The antibacterial activity of the extracts was compared with the antibacterial activity of xanthohumol, compound known for its broad pharmacological properties, isolated from the raw material remained after the SFE. Xanthohumol (XH, 96%) proved to posses the most prominent activity against all the tested strains, with the MIC values ranged between 2.5 and 20 μg mL-1. Supercritical hops extract and potassium isomerized supercritical hops extract showed strong antibacterial activity against the tested strains as well. Escherichia coli was not affected by the extracts, meaning that their oral admission would not cause the same problem as antibiotic app...

Journal of Ethnopharmacology

Pharmaceutics

The dual controlled release of emulgels makes them efficient drug delivery systems of increasing ... more The dual controlled release of emulgels makes them efficient drug delivery systems of increasing interest. The framework of this study was to incorporate selected L-ascorbic acid derivatives into emulgels. From the formulated emulgels, the release profiles of actives were evaluated considering their different polarities and concentrations, and consequently their effectiveness on the skin via a long-term in vivo study that lasted for 30 days was determined. Skin effects were assessed by measuring the electrical capacitance of the stratum corneum (EC), trans-epidermal water loss (TEWL), melanin index (MI) and skin pH. In addition, the sensory and textural properties of emulgel formulations were compared with each other. The changes in the rate of the release of the L-ascorbic acid derivatives were monitored using the Franz diffusion cells. The obtained data were statistically significant, and indicated an increase in the degree of hydration of the skin and skin whitening potential, wh...

Antioxidants

The aim of this study was to investigate the effect of the use of green solvents, natural deep eu... more The aim of this study was to investigate the effect of the use of green solvents, natural deep eutectic solvents (NaDES), in comparison with conventional solvents on the extraction of free unbound phenolic compounds and the antioxidant activity of extracts of dried bilberry fruit, bilberry leaves and green tea leaves. After preparation of the extracts via ultrasound-assisted extraction using NaDES and conventional solvents (water and ethanol), spectrophotometric determination of total phenolic and flavonoid content, HPLC analysis of extracted polyphenols and antioxidant determination using FRAP, DPPH and ABTS assays were conducted. The results showed that NaDES have a great potential as agents for the extraction of phenolic compounds with potent antioxidant activity; the highest values of phenolic content and antioxidant activity were detected in the samples obtained by extraction using the NaDES combination betaine + urea. The bilberry leaves exhibited the highest flavonoid content...

Journal of CO2 Utilization