Fatemeh Rostami | Tarbiat Modares University (original) (raw)
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Papers by Fatemeh Rostami
The effect of nanosilver was investigated on protein pattern changes, silver accumulation, altera... more The effect of nanosilver was investigated on protein pattern changes, silver accumulation, alterations in antioxidant capacity and reduced sugars in canola (Brassica napus L.) cultivar Ocapy under in vitro conditions. The grown seedlings on MS medium were subjected to MS medium containing 0, 0.5, 1, 1.5 and 2 ppm concentrations of silver nanoparticles for four weeks. Application of different concentrations of nanosilver had not significant effect on the total soluble protein in shoot but decreased protein content in 1.5 ppm concentration in root. The study of shoot and root protein patterns using one dimension gel electrophoresis (SDS-PAGE) and consequence analysis of bands by ImageJ program showed some remarkable changes. Relative expression of three proteins in shoot and five proteins in root were changes in response to nanosilver treatment. Silver accumulation was detected in shoot tissue only. There were some changes in antioxidant enzymes activity. The reduced sugars were increased in 1.5 ppm of nanosilver comparing with the control plants in shoot. It seems that the nanosilver at 1.5 ppm concentration is the best treatment of nanosilver as an ethylene action inhibitor under in vitro condition for B. napus L. cultivar Ocapy in this study.
Natural Science, 2010
We used SDS-PAGE to evaluate and characterize the protein patterns of seed storage proteins in fo... more We used SDS-PAGE to evaluate and characterize the protein patterns of seed storage proteins in four pistachios cultivars (Akbari, Ahmad Aghaei, Fandoghi, and Kaleghouchi). Total protein content of pistachio seeds in all cultivars did not show any significant difference. Results of SDS PAGE pattern of a few protein bands were up regulated whereas some other bands showed down regulation. The identified protein patterns may be used protein marker for pistachio cultivars.
We used SDS-PAGE to evaluate and characterize the protein patterns of seed storage proteins in fo... more We used SDS-PAGE to evaluate and characterize the protein patterns of seed storage proteins in four pistachios cultivars (Akbari, Ahmad Aghaei, Fandoghi, and Kaleghouchi). Total protein content of pistachio seeds in all cultivars did not show any significant difference. Results of SDS PAGE pattern of a few protein bands were up regulated whereas some other bands showed down regulation. The identified protein patterns may be used protein marker for pistachio cultivars.
The effect of nanosilver was investigated on protein pattern changes, silver accumulation, altera... more The effect of nanosilver was investigated on protein pattern changes, silver accumulation, alterations in antioxidant capacity and reduced sugars in canola (Brassica napus L.) cultivar Ocapy under in vitro conditions. The grown seedlings on MS medium were subjected to MS medium containing 0, 0.5, 1, 1.5 and 2 ppm concentrations of silver nanoparticles for four weeks. Application of different concentrations of nanosilver had not significant effect on the total soluble protein in shoot but decreased protein content in 1.5 ppm concentration in root. The study of shoot and root protein patterns using one dimension gel electrophoresis (SDS-PAGE) and consequence analysis of bands by ImageJ program showed some remarkable changes. Relative expression of three proteins in shoot and five proteins in root were changes in response to nanosilver treatment. Silver accumulation was detected in shoot tissue only. There were some changes in antioxidant enzymes activity. The reduced sugars were increased in 1.5 ppm of nanosilver comparing with the control plants in shoot. It seems that the nanosilver at 1.5 ppm concentration is the best treatment of nanosilver as an ethylene action inhibitor under in vitro condition for B. napus L. cultivar Ocapy in this study.
Natural Science, 2010
We used SDS-PAGE to evaluate and characterize the protein patterns of seed storage proteins in fo... more We used SDS-PAGE to evaluate and characterize the protein patterns of seed storage proteins in four pistachios cultivars (Akbari, Ahmad Aghaei, Fandoghi, and Kaleghouchi). Total protein content of pistachio seeds in all cultivars did not show any significant difference. Results of SDS PAGE pattern of a few protein bands were up regulated whereas some other bands showed down regulation. The identified protein patterns may be used protein marker for pistachio cultivars.
We used SDS-PAGE to evaluate and characterize the protein patterns of seed storage proteins in fo... more We used SDS-PAGE to evaluate and characterize the protein patterns of seed storage proteins in four pistachios cultivars (Akbari, Ahmad Aghaei, Fandoghi, and Kaleghouchi). Total protein content of pistachio seeds in all cultivars did not show any significant difference. Results of SDS PAGE pattern of a few protein bands were up regulated whereas some other bands showed down regulation. The identified protein patterns may be used protein marker for pistachio cultivars.