Characterization of seed storage protein patterns of four Iranian Pistachios using SDS-PAGE (original) (raw)

Evaluation of seed storage proteins in common bean by some biplot analysis

International Journal of Biosciences (IJB), 2013

In order to study of seed storage proteins, proteins samples of common bean genotypes were prepared by 0.2 M NaCl of extracting soluble. Genotypes were located in two groups by cluster analysis using Wilks' lambda statistic. Two groups were different for yield components (number of pods per plant, number of seeds per plant and seed weight). Factor analysis showed that two factors described 61% of total proteins variation. Correlated bands with yield components characters had the highest coefficients for the first factor. This factor was named "yield components proteins". Protein bands via RM 58 and 64 had relationship with days to flowering. Therefore, the second factor was named "phenologic proteins". Genotypes were located in four groups by these factors. Length, angle and presence of protein bands were important characteristics to explain graphical information in GGE biplot compared to factor analysis.

Seed Storage Protein Profile of Grain Legumes Grown in Iran, Using SDS-PAGE

Journal of Agricultural Science and Technology

Seed protein profiles of 47 accessions belonging to eleven species and four tribes of grain legumes were studied, by extracting the total proteins from ten single seeds in each accession and performing SDS-Polyacrylamide gel electrophoresis. All eleven species were clearly recognizable from their protein banding patterns, but only Phaseolus vulgaris ex-pressed high intraspecific variations, followed by Lathyrus sativus. Variation among ac-cessions of other species was very limited. Cluster analysis, after quantifying the protein bands, using UPGMA procedure, showed phylogenetic relationships which were in a good concordance with species classification based on morphological characters. Accessions of tribe Vicieae formed one cluster (Vicia faba, Lens culinaris, Pisum sativum, Lathyrus sati-vus and Vicia ervilia) having nearly equal amounts of three categories of polypeptide: high, moderate and low molecular weight. The second cluster was a small tribe of Cice-reae (Cicer arietinum ac...

Evaluation of seed storage protein patterns of ten wheat varieties using SDS-PAGE

BIHAREAN BIOLOGIST

SDS-PAGE was used to evaluate and characterize the patterns of seed storage proteins in ten bread wheat (Triticum aestivum L.) genotypes. Electrophorogram for each variety were scored for presence/absence in each sample, which were transformed into a binary data matrix. Jaccard΄s similarity coefficient, generated to determine the genetic similarity among genotypes, revealed that most of the genotypes were genetically distant from each other. The cluster analysis with UPGMA method separated the genotypes into 2 clusters. Based on Jaccard's similarity matrix, we represented a protein marker and it is clear that genotype 14GB & Hama-4, 14GB & un11 and Hama-4 & un11 have the highest similarity and lowest genetic distance. Genotypes Boomaz and Saji have the least similarity and maximum distance.

Differences in tomato seed protein profiles obtained by SDS-PAGE analysis

Journal of Agricultural Sciences, Belgrade, 2008

The protein profiles of tomato seeds from sub-species ( subsp. cultum Brezh., subsp. subspontaneum Brezh. and subsp. spontaneum Brezh.) were analyzed using SDS-PAGE technique. Electrophoreograms and denzitograms of total, soluble and non-soluble proteins of 31 different samples have showed quantitative and qualitative differences. Qualitative differences in electrophoregrams of total seed proteins refer to protein fragments in zone A (114 kDa, 83 kDa and 65 kDa) and protein fragment in zone C (17 kDa). Qualitative differences in electrophoregrams of soluble seed proteins refer to protein fragment in zone A (94 kDa). Qualitative differences in electrophoregrams of nonsoluble seed proteins refer to protein fragments with molecular weights of: 210 kDa, 85 kDa, 67 kDa and 26 kDa.

Analysis of Seed Proteins in Groundnut Cultivars (Arachis hypogaea L

The seed protein contents and protein banding pattern were studied in commonly cultivated groundnut cultivars. The groundnut cultivars such as ICGV00351, TMV-7, CO-4,CO-6 and TG-374 were used for quantitative and qualitative analysis of seed proteins. The protein contents varied among the different varieties of groundnut. The maximum protein content was observed in CO-6 followed by CO-4, TMV-7, ICGV00351 and TG-374. There was a slight differences in protein content among the different cultivars. All the five cultivars of groundnut were subjected to SDS-PAGE analysis. The results revealed that the variation in total number of bands and MW-Rf values. The maximum number of MW-Rf value was noticed in TG-374 and ICGV00351, and the minimum MW-Rf value was 11 recorded in CO-6 and TMV-7.

Characterization and Identification of Seed Storage Protein of Twelve Lettuce Cultivars

Biosciences, Biotechnology Research Asia, 2016

We used SDS-PAGE to evaluate and characterize the protein patterns of seed storage proteins in 12 lettuce cultivars. Total protein content of lettuce seeds in all cultivars did not show any significant difference. Results of SDS-PAGE pattern of a few protein bands were up regulated whereas some other bands showed down regulation. The identified protein patterns may be used protein marker for lettuce cultivars. The seed storage protein analyses helps in characterization and identification of diversity in lettuce crop varieties, cultivars and their wild varieties and also provides information on phylogenetic relationship of the accessions. It is also known that variation in protein bands provide information on the relationship among the used seeds. SDS-PAGE of seed protein using Tris-glycine buffer was performed to study the relationships within 12 cultivars of Lactuca sativa L. (lettuce). In the present study, SDS-PAGE was used to investigate and characterize the protein patterns of seed storage proteins in order to find protein bands as markers for cultivar characterization. Data were analyzed by clustering method and similarity coefficients using NTSYSpc version 2.02i. Thevalidity of cluster analysis of SDS-PAGE seed proteins profile data was discussed.

Evaluation of some cereals, plants and tubers through protein composition

Journal of protein chemistry, 1999

Wild and cultivated maize, sorghum, rice, amaranth, soybean, and cassava were screened for variability in seed storage proteins. Total seed proteins, albumin (Alb-1 and Alb-2), globulin, alcohol-soluble (A1 and A2), and glutelin (G1 and G2) fractions were investigated by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The comparison was done by the obtained protein patterns and their relative amounts. Using quantitative analysis of the protein composition and the electrophoretic patterns, the relationships between total proteins and amount of individual proteins were determined. Electrophoretic patterns of extracted proteins from investigated samples showed that the main protein subunits were concentrated between 10 and 45 kDa. Variation was found in major fractions and minor bands. Electrophoretic patterns of the protein fractions are directly related to the genetic background of the protein and can be identified and used to certify the genetic makeup...

Characterization of sunflower seed and kernel proteins

Helia, 2010

Total sunflower proteins, storage proteins, and helianthinin (11S) and 2S albumin fractions and their respective subunits in seeds and kernels of three sunflower hybrids were analyzed. Protein contents were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and coupled with densitometry. The SDS-PAGE profiles of the seed and kernel proteins in the crude extracts for all genotypes showed a very similar number of protein bands (thirty two) in the electrophoretograms. Three polypeptide groups of helianthinin fraction were detected. Two of these were acidic (α, Mw = 36,800 -42,900 Da and α', Mw = 31,000 -35,300 Da), while one was basic (β, Mw=21,000 -29,600 Da). The molecular weight of the 2S albumin proteins ranged from 11,500 to 20,100 Da. According to our results, there were significant differences among the seed and kernel protein contents. The 2S albumin content was significantly higher in kernels than in whole seeds of sunflower hybrids (P<0.05). By contrast, the 11S helianthinin content was significantly higher in seeds (where it ranged from 61.75 to 67.70% of totally extracted proteins) than in kernels (varied from 57.36 to 61.51% of totally extracted proteins) of sunflower hybrids (P<0.05).