Sumer Punia | Maharana Pratap University of Agriculture and Technology (original) (raw)
Papers by Sumer Punia
Genetic diversity among 100 accessions of chickpea collected from different agro-ecological zones... more Genetic diversity among 100 accessions of chickpea collected from different agro-ecological zones of India was assessed for several quantitative and qualitative traits. These accessions were grown in the augmented design with 5 intermittent checks viz., HK 94-134, KWR 108, GCP-105, Udai and Pant G-186 after every tenth row. Wide range of variability was observed for both qualitative and quantitative traits studied. All the chickpea genotypes were grouped into 11 discrete clusters with higher genetic diversity for different traits. Maximum 12 genotypes consisted by cluster II, IV, VII followed by cluster I, III and VI were having 11 genotypes. The highest intra-cluster distance was recorded for cluster II (23.214) followed by cluster VIII (16.218). The maximum inter-cluster distance was observed between cluster IV and XI (70.776) followed by cluster III and XI (58.599). The above result indicates that these genotypes having sufficient genetic diversity to generate segregants through crossing programme.
Genetic Resources and Crop Evolution, 2014
The lentil (Lens culinaris Medik.) is a selfpollinated seed legume with cleistogamous flowers. A ... more The lentil (Lens culinaris Medik.) is a selfpollinated seed legume with cleistogamous flowers. A spontaneous open flower semi leaf-less mutant, was observed from segregating generation (F4) of a cross IPL 313 9 RKL 1001 in which reproductive organs were not enclosed by the keel petals and thus remained exposed. Leaves on this mutant plant was very less and random with 1–3 pairs of leaf lets whereas in normal plant leaves are present at every reproductive node with 5–7 pairs of leaf lets. A very large number of open flowers ([90 %) remained sterile in mutant plant and their progenies, though its pollen fertility was as high as the standard cultivars. The progenies of the mutant open flower plant were segregates in three morphological different plant types as (1) open flower leafless plants, (2) open flower semi leaf-less plants and (3) normal plants having cleistogamous (closed) flowers and normal bearing leaves. The open flower leafless plant were low yielder whereas open flower semi leafless progenies have good yield potential and are important source of rare novel mutant alleles for important economic traits like multiple peduncles per reproductive node, more number of flowers/pods per peduncle and long reproductive phase. The open flower trait offers opportunity for exploring hybrid technology in the lentil and mutant alleles can play a crucial role in understanding the genetics of the target trait and for improvement of lentils. Keywords Leafless Lens culinaris Multiple peduncle Open flower Spontaneous mutant
Successful induction of autotetraploidy by seedling treatment of colchicine has been achieved in ... more Successful induction of autotetraploidy by seedling treatment of colchicine has been achieved in Lablab purpureus (L.) Sweet. Young seedlings were treated with different concentrations of aqueous colchicine (0.2, 0.3, 0.4 & 0.5%) using the cotton-swab method, each for different durations. Morphological and cytological variations were studied for C 1 and C 2 generations. In C 1 generation, 4 autotetraploids were obtained with 0.5% colchicine treatment for 6 and 9 h. The tetraploid plants were morphologically different as compared to diploids. Meiosis was irregular and average number of associations per cell ranged from 1.8±0.83 to 2.2±0.44 among quadrivalents and 24.00±0.00 to 19.6±0.89 in bivalents. Bivalent chromosome associations were observed more frequently than quadrivalent associations. Chiasma was not clearly seen. Seeds (10) from suspected tetraploids were used to raise the next generation to evaluate their stability. Only 5 plants were survived up to maturity and of these, one plant was suspected triploid and another plant monosomic. Thus morphological and cytogenetical changes in the polyploidy of L. purpureus, as a result of colchicine treatment, could not be significantly inherited in next generation and successful induction of stable tetraploids in next generation is a challenge for the researchers.
The lentil (Lens culinaris Medik.) is a selfpollinated seed legume with cleistogamous flowers. A ... more The lentil (Lens culinaris Medik.) is a selfpollinated
seed legume with cleistogamous flowers. A
spontaneous open flower semi leaf-less mutant, was
observed from segregating generation (F4) of a cross
IPL 313 9 RKL 1001 in which reproductive organs
were not enclosed by the keel petals and thus remained
exposed. Leaves on this mutant plant was very less and
random with 1–3 pairs of leaf lets whereas in normal
plant leaves are present at every reproductive node
with 5–7 pairs of leaf lets. A very large number of open
flowers ([90 %) remained sterile in mutant plant and
their progenies, though its pollen fertility was as high
as the standard cultivars. The progenies of the mutant
open flower plant were segregates in three morphological
different plant types as (1) open flower leafless
plants, (2) open flower semi leaf-less plants and
(3) normal plants having cleistogamous (closed)
flowers and normal bearing leaves. The open flower
leafless plant were low yielder whereas open flower
semi leafless progenies have good yield potential and
are important source of rare novel mutant alleles for
important economic traits like multiple peduncles per
reproductive node, more number of flowers/pods per
peduncle and long reproductive phase. The open
flower trait offers opportunity for exploring hybrid
technology in the lentil and mutant alleles can play a
crucial role in understanding the genetics of the target
trait and for improvement of lentils.
Keywords Leafless Lens culinaris Multiple
peduncle Open flower Spontaneous mutant
The extent of genetic variation obtained naturally is useful in self-pollinated crops like lentil... more The extent of genetic variation obtained naturally is useful in self-pollinated crops like lentil, though the variation is very low in frequency. A single plant was isolated from a commercial population of DPL-62 in 2007-08 assuming that it is a mutant of DPL-62 with earliness and other morphological traits. Interestingly, the progenies of the isolated plant showed segregation up to sixth generation (rabi 2012-13). Wide variation was observed for days to flowering (38-66), days to maturity (95-135), reproductive phase (55-80 days), plant height (35-65 cm), pods per plant (30-185), 100seed weight (3.10 to 6.80 g), seed coat colour and other traits. Mutation at multiple loci may be the possible reason. In every generation segregation has been seen for major traits, and still the material is segregating which shows a kind of dynamic mutation. P resence of mobile genetic elements in the functional genome may also be a reason. This genetic stock can play a crucial role in understanding the genetics of the target trait and improvement of small-seed Indian lentils.
Genetic diversity among 100 accessions of chickpea collected from different agro-ecological zones... more Genetic diversity among 100 accessions of chickpea collected from different agro-ecological zones of India was assessed for several quantitative and qualitative traits. These accessions were grown in the augmented design with 5 intermittent checks viz., HK 94-134, KWR 108, GCP-105, Udai and Pant G-186 after every tenth row. Wide range of variability was observed for both qualitative and quantitative traits studied. All the chickpea genotypes were grouped into 11 discrete clusters with higher genetic diversity for different traits. Maximum 12 genotypes consisted by cluster II, IV, VII followed by cluster I, III and VI were having 11 genotypes. The highest intra-cluster distance was recorded for cluster II (23.214) followed by cluster VIII (16.218). The maximum inter-cluster distance was observed between cluster IV and XI (70.776) followed by cluster III and XI (58.599). The above result indicates that these genotypes having sufficient genetic diversity to generate segregants through crossing programme.
Genetic Resources and Crop Evolution, 2014
The lentil (Lens culinaris Medik.) is a selfpollinated seed legume with cleistogamous flowers. A ... more The lentil (Lens culinaris Medik.) is a selfpollinated seed legume with cleistogamous flowers. A spontaneous open flower semi leaf-less mutant, was observed from segregating generation (F4) of a cross IPL 313 9 RKL 1001 in which reproductive organs were not enclosed by the keel petals and thus remained exposed. Leaves on this mutant plant was very less and random with 1–3 pairs of leaf lets whereas in normal plant leaves are present at every reproductive node with 5–7 pairs of leaf lets. A very large number of open flowers ([90 %) remained sterile in mutant plant and their progenies, though its pollen fertility was as high as the standard cultivars. The progenies of the mutant open flower plant were segregates in three morphological different plant types as (1) open flower leafless plants, (2) open flower semi leaf-less plants and (3) normal plants having cleistogamous (closed) flowers and normal bearing leaves. The open flower leafless plant were low yielder whereas open flower semi leafless progenies have good yield potential and are important source of rare novel mutant alleles for important economic traits like multiple peduncles per reproductive node, more number of flowers/pods per peduncle and long reproductive phase. The open flower trait offers opportunity for exploring hybrid technology in the lentil and mutant alleles can play a crucial role in understanding the genetics of the target trait and for improvement of lentils. Keywords Leafless Lens culinaris Multiple peduncle Open flower Spontaneous mutant
Successful induction of autotetraploidy by seedling treatment of colchicine has been achieved in ... more Successful induction of autotetraploidy by seedling treatment of colchicine has been achieved in Lablab purpureus (L.) Sweet. Young seedlings were treated with different concentrations of aqueous colchicine (0.2, 0.3, 0.4 & 0.5%) using the cotton-swab method, each for different durations. Morphological and cytological variations were studied for C 1 and C 2 generations. In C 1 generation, 4 autotetraploids were obtained with 0.5% colchicine treatment for 6 and 9 h. The tetraploid plants were morphologically different as compared to diploids. Meiosis was irregular and average number of associations per cell ranged from 1.8±0.83 to 2.2±0.44 among quadrivalents and 24.00±0.00 to 19.6±0.89 in bivalents. Bivalent chromosome associations were observed more frequently than quadrivalent associations. Chiasma was not clearly seen. Seeds (10) from suspected tetraploids were used to raise the next generation to evaluate their stability. Only 5 plants were survived up to maturity and of these, one plant was suspected triploid and another plant monosomic. Thus morphological and cytogenetical changes in the polyploidy of L. purpureus, as a result of colchicine treatment, could not be significantly inherited in next generation and successful induction of stable tetraploids in next generation is a challenge for the researchers.
The lentil (Lens culinaris Medik.) is a selfpollinated seed legume with cleistogamous flowers. A ... more The lentil (Lens culinaris Medik.) is a selfpollinated
seed legume with cleistogamous flowers. A
spontaneous open flower semi leaf-less mutant, was
observed from segregating generation (F4) of a cross
IPL 313 9 RKL 1001 in which reproductive organs
were not enclosed by the keel petals and thus remained
exposed. Leaves on this mutant plant was very less and
random with 1–3 pairs of leaf lets whereas in normal
plant leaves are present at every reproductive node
with 5–7 pairs of leaf lets. A very large number of open
flowers ([90 %) remained sterile in mutant plant and
their progenies, though its pollen fertility was as high
as the standard cultivars. The progenies of the mutant
open flower plant were segregates in three morphological
different plant types as (1) open flower leafless
plants, (2) open flower semi leaf-less plants and
(3) normal plants having cleistogamous (closed)
flowers and normal bearing leaves. The open flower
leafless plant were low yielder whereas open flower
semi leafless progenies have good yield potential and
are important source of rare novel mutant alleles for
important economic traits like multiple peduncles per
reproductive node, more number of flowers/pods per
peduncle and long reproductive phase. The open
flower trait offers opportunity for exploring hybrid
technology in the lentil and mutant alleles can play a
crucial role in understanding the genetics of the target
trait and for improvement of lentils.
Keywords Leafless Lens culinaris Multiple
peduncle Open flower Spontaneous mutant
The extent of genetic variation obtained naturally is useful in self-pollinated crops like lentil... more The extent of genetic variation obtained naturally is useful in self-pollinated crops like lentil, though the variation is very low in frequency. A single plant was isolated from a commercial population of DPL-62 in 2007-08 assuming that it is a mutant of DPL-62 with earliness and other morphological traits. Interestingly, the progenies of the isolated plant showed segregation up to sixth generation (rabi 2012-13). Wide variation was observed for days to flowering (38-66), days to maturity (95-135), reproductive phase (55-80 days), plant height (35-65 cm), pods per plant (30-185), 100seed weight (3.10 to 6.80 g), seed coat colour and other traits. Mutation at multiple loci may be the possible reason. In every generation segregation has been seen for major traits, and still the material is segregating which shows a kind of dynamic mutation. P resence of mobile genetic elements in the functional genome may also be a reason. This genetic stock can play a crucial role in understanding the genetics of the target trait and improvement of small-seed Indian lentils.