Kumud Harley | MUHS, Nasik (original) (raw)

Papers by Kumud Harley

PARIPEX INDIAN JOURNAL OF RESEARCH, Jan 15, 2024

Background Dyslipidemia is frequently have lipoprotein abnormalities such as low HDL-C concentrat... more Background Dyslipidemia is frequently have lipoprotein abnormalities such as low HDL-C concentrations, increased remnant particles and hypertriglyceridemia. HDL-C concentrations are inversely correlated with atherogenic risk. PON-1 activity was lower in patients with renal insufficiency Aim & Objectives 1) To evaluate activity of PON-1 activity in patients with CRF during pre-and post-hemodialysis sessions 2) To establish interrelationships if any, between PON-1, blood urea and serum creatinine levels. 3) To identify the association of PON-1 with serum HDL levels among CRF patients Materials And Methods Hospital based case-control study consists of 40 patients who were receiving Hemodialysis (HD) therapy in the dialysis unit. Study designed to evaluate alterations in serum PON-1 activity in patients during pre and post dialysis sessions in CRF. About 17% increase in the activities of PON-1 have been evident during post-Result hemodialysis as compared to pre-hemodialysis samples of patients with CRF. The mean activity of PON-1 and HDL were decreased in pre-hemodialysis samples of patients with CRF. PON-1 activity and HDL was increased in CRF patients during post-hemodialysis as compared to pre-hemodialysis sessions. In both, the results were statistically highly significant (p < 0.001). There is a positive correaltion between HDL and PON 1. This study demonstrates that Conclusion there is an increased risk of cardiovascular complications in patients undergoing hemodialysis. CRF patients undergoing hemodialysis show an increased generation of reactive oxygen species accompanied by an altered lipid profile and paraoxonase-1.

International Journal of Clinical Biochemistry and Research, 2017

Background: Arterial blood gas analysis is a common investigation exercised in emergency room and... more Background: Arterial blood gas analysis is a common investigation exercised in emergency room and ICU. In post-operative CABG patients, acid base alterations is the commonest problem encountered. Thus, constant check on these parameters is necessary to minimize post-operative mortality. Aim and Objectives: To document the pre-operative and post-operative ABG, hemoglobin and serum potassium in patients undergoing CABG. Materials and Method: Present observational study was conducted on 27 patients (Male=12 & Female=15) in the department of clinical biochemistry and cardiovascular thoracic unit at GMC-SSH, Nagpur. Fresh Arterial blood Samples were collected in dept. of biochemistry for ABG, hemoglobin and serum potassium pre-operatively and post operatively. Data were expressed in mean and standard deviation. Level of significance was expressed in P value applying student paired “t” tests. Results: Findings suggested significant decrease in PaO2, in post-operative 5 & 9 day (P=0.0001) ...

International Journal of Clinical Biochemistry and Research, Mar 15, 2018

Introduction: Nitric oxide (NO) is known as a mediator in a broad cluster of biological systems. ... more Introduction: Nitric oxide (NO) is known as a mediator in a broad cluster of biological systems. NO is the endothelium-derived relaxing factor, which causes vasodilation by relaxing vascular smooth muscle. This role of NO plays an important role of diabetic nephropathy in Type 2 Diabetes Mellitus (DM) Aims: To assess serum nitric oxide levels among type 2 diabetes mellitus patients having normoalbuminuria and microalbuminuria. Setting and Design: This cross sectional study was carried out at Department of Biochemistry in a tertiary care rural hospital in Central India with 200 diagnosed patients of type 2 diabetes mellitus. Materials and Methods: 24 hr urine albumin was done in all the patients. Based on the concentration of albumin in urine, patients were categorised in two groups, group 1 had normoalbuminuria (i.e. Urine Albumin <20 mg/ 24 hr) and group 2 had microalbuminuria (i.e. Urine Albumin >20 to <300 mg/24 hr). Then, serum NO was assessed and compared in these groups. Results: Serum NO levels were significantly raised (p <0.05) in Group 2 as compared to Group 1. Conclusion: Our observations suggest that serum NO levels measurement has a good detection potential for early stages of diabetic nephropathy manifested in the form of microalbuminuria in Type 2 DM

Abstract. COVID-19 is an infection induced by the SARS-CoV-2 virus, that has resulted in a worldw... more Abstract. COVID-19 is an infection induced by the SARS-CoV-2 virus, that has resulted in a worldwide sanitary crisis. COVID-19 has a wide variety of clinical features, ranging from asymptomatic infection to minor to severe pneumonia. Different laboratory markers get altered in these patients, according to recent studies, and are therefore valuable as biomarkers to detect disease development and identify patients who may present a severe and/or deadly clinical condition. This article reviews biochemistry and immunology biomarkers that are changed in COVID-19 positive individuals, as well as inflammatory markers, and their influence on liver, heart, kidney and pancreatic functions markers' levels, as well as their significance in the disease's progression.

Objectives - The aim was to study association of blood lipids and Atherogenic index of plasma be... more Objectives - The aim was to study association of blood lipids and Atherogenic index of plasma between hypothyroid and controls. Material and Methods - Thyroid function test and lipid profile were done in 50 hypothyroid cases and compaired with controls. AIP was calculated as log TG/HDL-C using CZECH online calculator of Atherogenic risk. Result - We found there is significant elevation in levels of cholesterol, Triglycerides, LDL,HDL,VLDL in hypothyroidism as compared to control. The increase in mean levels of AIP in hypothyroid (0.280±0.108) was highly significant statistically ( p value < 0.0001) as compared to controls (0.165±0.099). Our AIP results indicates controls may have moderate risk for CVD though they have normal lipid profile and need to be screened for dyslipidemia and AIP. Conclusion- TheAtherogenic index of plasma is significantly elevated hypothyroidism along with standard lipids, hence it can be used for risk assessment of atherosclerosis in hypothyroid p...

Ukrainian Journal of Nephrology and Dialysis

COVID-19 is an infection induced by the SARS-CoV-2 virus, that has resulted in a worldwide sanita... more COVID-19 is an infection induced by the SARS-CoV-2 virus, that has resulted in a worldwide sanitary crisis. COVID-19 has a wide variety of clinical features, ranging from asymptomatic infection to minor to severe pneumonia. Different laboratory markers get altered in these patients, according to recent studies, and are therefore valuable as biomarkers to detect disease development and identify patients who may present a severe and/or deadly clinical condition. This article reviews biochemistry and immunology biomarkers that are changed in COVID-19 positive individuals, as well as inflammatory markers, and their influence on liver, heart, kidney and pancreatic functions markers' levels, as well as their significance in the disease's progression.

Journal of Pharmaceutical Research International

Type 2 diabetes mellitus is becoming more common over the world, making it a major health concern... more Type 2 diabetes mellitus is becoming more common over the world, making it a major health concern. Many research has been carried a link among both higher uric acid concentrations in the body and a higher incidence of type 2 diabetes mellitus. On a biochemical level, uric acid has been proved to have a negative impact on insulin sensitivity in animals. When nitric oxide, essential for glucose uptake, is suppressed, this occurs. The goal of this study is to review past research on the relationship between serum uric acid and diabetes type II in various parts of the world, as well as the prevalence of such a link. Articles were chosen using the PubMed database and EBSCO Information Services. We used all relevant publications to our review that dealt with the connection among both serum uric acid and diabetes type II, in addition to the other articles. Other publications that were not linked to this field were excluded. In this systematic review, serum uric Acid levels were found to be...

Journal of Evolution of Medical and Dental Sciences

BACKGROUND Diabetes mellitus is a long-lasting illness, frequently getting diagnosed when complic... more BACKGROUND Diabetes mellitus is a long-lasting illness, frequently getting diagnosed when complications appear. Natural course of diabetic nephropathy can be seen with normoalbuminuria to microalbuminuria, then to chronic renal disease through overt proteinuria. Microalbuminuria is a renal marker of generalized vascular endothelial damage and early atherosclerosis. Patients having microalbuminuria are at higher risk of vascular complications of diabetes mellitus like myocardial infarction, cerebrovascular accident and diabetic nephropathy. Poor glycaemic control increases the risk of microalbuminuria. This is the reason why urinary albumin is used as a sensitive marker of diabetic nephropathy. The present study aimed to correlate the HbA1c levels with urinary albumin in subjects of type 2 diabetes mellitus. METHODS A cross sectional study was done at a tertiary care hospital. We studied 200 diagnosed subjects of type 2 diabetes mellitus. Depending on their urinary albumin excretion, they were categorized into 2 groups-groups 1 and 2. Group 1 and Group 2 subjects had a mean age of 43.75 ± 5.95 years and 51.06 ± 7.94 years respectively. Group 1 and 2 subjects had 7.06 ± 0.56 (%) and 8.27 ± 1.30 (%) of HbA1c respectively. RESULTS HbA1c levels were compared in Group 1 (7.06 ± 0.56) and Group 2 (8.27 ± 1.30) and the difference was statistically significant (p value = 0.0001, S). We found positive correlation between urinary albumin and HbA1c in Groups 1 (r = 0.255) and Group 2 (r = 0.417). CONCLUSIONS Constant rise in glycated haemoglobin and micro albuminuria may be considered as risk factors in diabetic nephropathy. Therefore, regular screening for HbA1c and urinary albumin must be done as an early marker of renal dysfunction.

International Journal of Clinical Biochemistry and Research

Introduction: Advances in science and Technology have led to transformation of laboratory diagnos... more Introduction: Advances in science and Technology have led to transformation of laboratory diagnostics from manual, clumsy testing methods to fully automated science, ensuring accuracy and speed. Pre analytical errors have a major impact on diagnostic accuracy of laboratory results. There have been tremendous work and established quality control criteria for analytical phase of testing however there is paucity of standards for pre analytical phase. Quality indicators (QIs) should therefore cover all the steps involved in the pre-analytical phase, from test requesting, transport to sample storage. Objectives: The following were the objectives for the study: 1. To discern the percent age of pre-analytical errors in our central clinical biochemistry Laboratory (CCL); 2. To stratify the pre-analytical errors documented at CCL; 3. To formulate the possible corrective measures to be taken to minimise such errors. Materials and Methods: In patients (IPD) 18,982 blood specimens requested and received at CCL for various biochemical investigations during November 2018 to May 2019 (6 months) were first sorted out for pre analytical errors. And n= 1907 blood specimens were identified with pre analytical errors were further stratified and categorised according to the error contributing and expressed in percentage. Result: Total 1907 blood specimens were documented and grouped under pre analytical phase errors out of 18,982 total samples received at CCL. When sorted for individual pre-analytical error, out of total n= 1907; Improper request form (n= 107), incorrect timing of sample (n=37), improper labelling (n=65); improper tube collection (n=67) ; insufficient sample (n=228) and in-vitro haemolysis (n=251), sample not received (SNR) (n=1142) of samples amounted to be the major proportion of errors. Conclusion: Pre-analytical errors are not inevitable and can be avoided with a diligent application of proper quality control, proper education of phlebotomist about the errors and effective collection systems to improve the total quality management of laboratory so as to ensure total quality patient care.

Aim: To determine the alteration in total nitric oxide (NO), nitrate (NO)), nitrite (N0 2), coppe... more Aim: To determine the alteration in total nitric oxide (NO), nitrate (NO)), nitrite (N0 2), copper (Cu) and zinc (In) in serum and Cu/In ratio and the role they may play in contributing to breast and other cancers. Materials and methods: The present study was conducted in total of 90 subjects divided into three groups as control (n=30), breast cancer (n=30), and other cancers (n=30). Serum NO and NO) was estimated by cadmium reduction method. Serum Cu and In were estimated by atomic absorption spectrometry. Serum N0 2 and Cu/In ratio were calculated. Results: Total NO, Cu and Cu/In ratio were significantly raised (p< 0.0001) while In levels were significantly low (p< 0.0001), in serum of both breast and other cancer patients as compared to the healthy controls. Fall in In level was more in other cancer patients as compared to the breast cancer patients (p< 0.05). Serum NO) levels were significantly more in other cancer patients (p< 0.0001) while N0 2 levels were raised i...

The levels of cerebrospinal fluid total cholesterol, triglycerides, sodium, potassium and inorgan... more The levels of cerebrospinal fluid total cholesterol, triglycerides, sodium, potassium and inorganic phosphorous were measured in 21 patients with neurological disorders (tuberculous meningitis (TBM), hydrocephalous and TBM with hydrocephalous) and in healthy matched controls. The concentration of total cholesterol, triglycerides, sodium, potassium, inorganic phosphorous were significantly higher in CSF of all the three study groups than in control groups. The determination of CSF total cholesterol, triglycerides, sodium, potassium, inorganic phosphorous may aid in the diagnosis of TBM, hydrocephalous and TBM complicated hydrocephalous patients.

Background: Proper use of teaching aids helps to retain more concepts permanently. Students can l... more Background: Proper use of teaching aids helps to retain more concepts permanently. Students can learn better when they are motivated properly through different teaching aids, such as chalkboards and power point presentations are commonly used to pique students' interest and demonstrate how things work, to clarify better and can facilitate the proper understanding, help to keep classroom live and active. Present study was thus conducted to investigate perception of conventional chalkboard and modern power point teaching methods among 1st M.B.B.S. students of Mahatma Gandhi Institute of Medical Sciences, Sevagram. Methods: 65 students (Male=32, Female=33) of 1 st MBBS (2013-14 Batch) were administered with Questionnaire comprised of 10 questions. Statistical analysis was done by using chi-square test.Results: Total 73.85 % students preferred chalkboard as the superior teaching tool compared to Power Point presentation. Statistically significant difference of opinion observed betwe...

Background: Several studies have been carried out to evaluate the plasma Adiponectin levels and i... more Background: Several studies have been carried out to evaluate the plasma Adiponectin levels and its associations with chronic heart disease. However, limited data is available regarding the serum Adiponectin levels and the Acute Myocardial infarction (AMI) especially in central India. Material and Methods: The present cross sectional study was conducted at tertiary care hospital in central India. Study groups comprised of 101 AMI cases and 100 age and sex matched apparently healthy controls to assess the status of serum Adiponectin and lipid parameters. Observations: We have observed significantly low serum Adiponectin levels in AMI patients as compared to controls with P value 0.0001. In addition, when compared with lipid parameters, Adiponectin levels were positively related with high density lipoprotein cholesterol, whereas, negatively correlate with the serum triglycerides, Total Cholesterol and Low density lipoprotein Cholesterol. In addition when compared lipid parameters betw...

International Journal of Medical and Clinical Research, 2011

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Selenium is a trace element that plays role in antioxidant defense mechanism via selenium depende... more Selenium is a trace element that plays role in antioxidant defense mechanism via selenium dependent glutathione peroxidases (GSH-PX) and selenoproteins. When selenium is lacking, the intima of the arteries is prone to get oxidative damage and involve in development of atherosclerosis. It has been observed in clinical studies that the serum selenium levels in patients with myocardial infarction and with angina pectoris have been significantly lower than that in healthy people. In the present study we found significantly low concentration of serum selenium in acute myocardial infarction (AMI) compared with the age and sex matched healthy controls with p value less than 0.001.