John Fagan | Maharishi University of Management (original) (raw)

Papers by John Fagan

Journal of Biological Chemistry, 1993

Journal of Biological Chemistry, 1981

Biochemical and Biophysical Research Communications, Aug 1, 1981

... Biophys Res. Commun. 82, 129135 9. Goodwin, GH, Walker, JM and Johns, EW, (1978) The Cell Nuc... more ... Biophys Res. Commun. 82, 129135 9. Goodwin, GH, Walker, JM and Johns, EW, (1978) The Cell Nucleus (Busch H. ed) Vol. 6, 182221, Academic Press 10. ... Reischsteiner, M., and Kuehl, Le Roy, (1979) Cell 16, 901908 14. Adesnik, M., and Darnell, JE, (1972) J. Mol. Biol. ...

Medicina

Background and Objectives: Stress can overload adaptive mechanisms, leading to epigenetic effects... more Background and Objectives: Stress can overload adaptive mechanisms, leading to epigenetic effects harmful to health. Research on the reversal of these effects is in its infancy. Early results suggest some meditation techniques have health benefits that grow with repeated practice. This study focused on possible transcriptomic effects of 38 years of twice-daily Transcendental Meditation® (TM®) practice. Materials and Methods: First, using Illumina® BeadChip microarray technology, differences in global gene expression in peripheral blood mononuclear cells (PBMCs) were sought between healthy practitioners and tightly matched controls (n = 12, age 65). Second, these microarray results were verified on a subset of genes using quantitative polymerase chain reaction (qPCR) and were validated using qPCR in larger TM and control groups (n = 45, age 63). Bioinformatics investigation employed Ingenuity® Pathway Analysis (IPA®), DAVID, Genomatix, and R packages. Results: The 200 genes and loci ...

Biological Agriculture & Horticulture, 2019

Rosemary (Rosmarinus officinalis L.) is an important medicinal and aromatic herb, used by various... more Rosemary (Rosmarinus officinalis L.) is an important medicinal and aromatic herb, used by various pharmaceutical, food and cosmetics industries. For most species, seed-based multiplication is effective and convenient, but for rosemary, seed germination is a problem due to the mucilaginous seed coat, and sometimes the germination is no more than 10-20% resulting in non-uniform crop establishment. This investigation was carried out to improve seed germination by using low-cost organic seed priming techniques. Eleven pre-sowing seed treatments were tested, based on locally available resources, including: soaking seeds in water (cold and hot), bovine urine (5%, 10%, 15% and 20% concentration), bovine dung slurry, buttermilk, compost tea and Agnihotra ash, and comparing with an untreated control. All the pre-sowing seed treatments increased the percentage of seed germination in rosemary compared with the control, except buttermilk, which had an adverse effect. Among the different treatments, seed germination was significantly increased to 46.7% for seeds treated with bovine dung slurry and to 42.0% for seeds treated with bovine urine 5.0% , compared with those in the control where the germination was 9.7%. The bovine dung slurry treatment also recorded uniform and early germination (9 days for initiation and completion at 43 days) resulting in a significantly higher rate of germination (1.4) than the control. The number of leaves, the root length and the seedling vigour were also recorded to be the highest for seeds treated with bovine dung slurry, followed by bovine urine 5.0% .

Journal of Biological Chemistry, 1990

Foods, 2022

The stated objective of the paper was to provide regulatory laboratories and industry laboratorie... more The stated objective of the paper was to provide regulatory laboratories and industry laboratories a complete, legally robust method for the quantitative detection of SU canola [...]

Foods, 2020

Discussion regarding the regulatory status of genome-edited crops has focused on precision of edi... more Discussion regarding the regulatory status of genome-edited crops has focused on precision of editing and on doubts regarding the feasibility of analytical monitoring compliant with existing GMO regulations. Effective detection methods are important, both for regulatory enforcement and traceability in case of biosafety, environmental or socio-economic impacts. Here, we approach the analysis question for the first time in the laboratory and report the successful development of a quantitative PCR detection method for the first commercialized genome-edited crop, a canola with a single base pair edit conferring herbicide tolerance. The method is highly sensitive and specific (quantification limit, 0.05%), compatible with the standards of practice, equipment and expertise typical in GMO laboratories, and readily integrable into their analytical workflows, including use of the matrix approach. The method, validated by an independent laboratory, meets all legal requirements for GMO analyti...

1. GM crops do not increase yield potential Genetically modified (GM) crops do not increase yield... more 1. GM crops do not increase yield potential Genetically modified (GM) crops do not increase yield potential and sometimes decrease it. While the yields of major crops have increased over recent decades, this is due to conventional breeding, not GM.1 High yield is a complex genetic trait resulting from many genes working together in ways that are not fully understood by scientists. It cannot be genetically engineered into crops with the existing crude techniques – or with any techniques in the development pipeline. Good farming methods, such as maintaining soil fertility, are equally or more important to maximizing yields. A study comparing agricultural productivity in the United States and Western Europe over the last 50 years, focusing on the staple crops of maize, canola, and wheat, found that the US’s mostly GM production was lowering yields and increasing pesticide use compared to Western Europe’s mostly non-GM production. Contrary to claims that Europe’s reluctance to embrace G...

Nature Biotechnology, 1999

Sage (Salvia officinalis L.) is an important medicinal and aromatic herb, used as a raw material ... more Sage (Salvia officinalis L.) is an important medicinal and aromatic herb, used as a raw material for various perfumery, pharmaceutical, food, and cosmetics industries. S. officinalis belonging to the family Lamiaceae is commercially cultivated for the essential oil and is grown throughout Europe and the United States, and in parts of Turkey, Yugoslavia, and Canada. Leaves are highly aromatic, used for extraction of essential oil, which contains more than 49 aromatic components. The principal components in the sage oil are 1, 8-cineole, camphor, α-thujone, β-thujone, α-humulene, rosmarinic acid, and quercetin. Traditionally garden sage has been used for the treatment of a multitude of ailments such as localized pain, rheumatism, convulsion, arthritis, vertigo, diarrhea, sclerosis, respiratory, metabolic and mental disorders. As the herbal medicines gaining importance in recent years, usage and systematic documentation of such herb are found essential. Thus an effort has been made to ...

Molecules, 2020

Rosmarinus officinalis is a potent antioxidant herb rich in polyphenols. Ultra-high-performance l... more Rosmarinus officinalis is a potent antioxidant herb rich in polyphenols. Ultra-high-performance liquid chromatography, coupled with electrospray ionization and quadrupole-time of flight mass spectrometry (UHPLC-ESI-QTOF-MS), enables an exhaustive, full-spectrum analysis of the molecular constituents of natural products. The study aimed to develop a rapid UHPLC method to contribute new insights into the phytochemical composition of rosemary and to assess the performance of nine different procedures for extraction. These include fresh tissue homogenization, fresh and dry leaf decoction, and their respective fermentation, Soxhlet extraction, and sonication using water and methanol. Different extraction methods were found to recover quite different groups of polyphenols within 11 min during 20 min of analysis. Soxhlet extraction, yielded very high concentrations of rosmarinic acid (33,491.33 ± 86.29 µg/g), luteolin-7-O-glucoside (209.95 ± 8.78 µg/g), carnosic acid (2915.40 ± 33.23 µg/g)...

Planta Medica International Open, 2020

UHPLC with QTOF-MS is widely used as a powerful tool for metabolomic analysis. This technology ha... more UHPLC with QTOF-MS is widely used as a powerful tool for metabolomic analysis. This technology has recently been applied to the analysis of polyphenols in food and herb extracts. Sage (Salvia officinalis) and rosemary (Rosmarinus officinalis), belonging to the family Lamiaceae, are known for their potent antioxidant properties due to the presence of polyphenols. We have developed a sensitive and reproducible UHPLC-QTOF-MS/MS-based method for comprehensive phytochemical profiling and the identification and quantitation of specific polyphenolic compounds present in sage and rosemary leaves. The herbs were extracted ultrasonically using methanol as the solvent. In sage, rosmarinic acid (17 678.7±673.4 µg/g) and 12-methoxy carnosic acid (21 918.3±715.4 µg/g) were found in the highest concentrations among all polyphenols. In contrast, rosmarinic acid (14 311.0±636.4 µg/g), luteolin-3'-acetyl-O-glucuronide (1488.50±47.58 µg/g), and luteolin-7-O-glucuronide (1053.68±68.83 µg/g) were ob...

Molecular and Cellular Biology, 1985

We wished to determine whether simian virus 40 (SV40)-transformed xeroderma pigmentosum cells, de... more We wished to determine whether simian virus 40 (SV40)-transformed xeroderma pigmentosum cells, despite their defective DNA repair, were suitable for DNA-mediated gene transfer experiments with linked genes. Expression of a nonselectable gene (cat, coding for chloramphenicol acetyltransferase [CAT]) linked to a selectable gene (gpt, coding for xanthine-guanine phosphoribosyltransferase [XPRT]) in the plasmid pSV2catSVgpt was quantified after transfection of SV40-transformed xeroderma pigmentosum [XP20s(SV40)] and normal human [GM0637(SV40)] fibroblast cell lines. A novel autoradiographic assay with [3H]xanthine incorporation showed 0.5 to 0.7% phenotypic expression of XPRT in both cell lines. Without selection, transient CAT activity was 20 times greater in the GM0637(SV40) than in the XP20s(SV40) cells, and transient XPRT activity was 5 times greater. Both of these transient activities were increased and equalized in both cell lines by transfection with pRSVcat or pRSVgpt. Genotypic...

American Journal of Plant Sciences, 2016

Molecular and Cellular Biology, 1990

Three nuclear factors, the Ah receptor, XF1, and XF2, bind sequence specifically to the Ah respon... more Three nuclear factors, the Ah receptor, XF1, and XF2, bind sequence specifically to the Ah response elements or xenobiotic response elements (XREs) of the cytochrome P450IA1 (P450c) gene. The interactions of these factors with the Ah response element XRE1 were compared by three independent methods, methylation interference footprinting, orthophenanthroline-Cu+ footprinting, and mobility shift competition experiments, using a series of synthetic oligonucleotides with systematic alterations in the XRE core sequence. These studies established the following (i) all three factors interact sequence specifically with the core sequence of XRE1; (ii) the pattern of contacts made with this sequence by the Ah receptor are different from those made by XF1 and XF2; and (iii) although XF1 and XF2 can be distinguished by the mobility shift assay, the sequence specificities of their interactions with XRE1 are indistinguishable. Further characterization revealed the following additional differences ...

Agronomski Glasnik, Oct 15, 2008

Environmental Sciences Europe, 2015