Samaneh Boroumand-Noughabi | Mashhad University of Medical Sciences, Mashhad. Iran. (original) (raw)

Papers by Samaneh Boroumand-Noughabi

Transfusion Clinique Et Biologique, Dec 1, 2021

Biomedicine & Pharmacotherapy, 2022

Transfusion and Apheresis Science, 2022

Hemolytic disease of the fetus and newborn (HDFN) is a clinically significant problem that may po... more Hemolytic disease of the fetus and newborn (HDFN) is a clinically significant problem that may potentially affect any pregnancy. Direct antiglobulin test (DAT) is considered to be an important test in identifying newborns who are suspected to have HDN. This study aims in reviewing data regarding a positive DAT result concerning etiology and the development of HDN over a period of 10 years. A retrospective study of all neonates with a positive DAT result between January 2011 and December 2020 was performed. Data were obtained from patients' electronic hospital files, transfusion medicine databases, and medical birth records. Laboratory parameters along with clinical interventions in neonates with a DAT-positive result and a comparison group of DAT-negative neonates were performed. 36,000 deliveries were registered in this period. 176 (2.65 %) neonates had a positive DAT result. ABO-incompatibility was the most common cause with 59.1 %; Rh incompatibility 13.8 %, minor blood group incompatibility, and other RBC-related antibodies 10.1 %, and unspecified etiology in 17 % of cases. Among DAT-positive cases, 32.7 % of neonates were diagnosed with HDN. ABO-incompatibility was the major reason as well. Initial mean total bilirubin levels were higher in the DAT-positive group than the control group (p < 0.001), and these neonates also had a lower initial hemoglobin level (p < 0.001). The need for therapeutic interventions was significantly higher in DAT-positive neonates (p < 0.001) as 86.8 % underwent phototherapy, with 32.7 %, and 17.6 % receiving exchange transfusion (ET) and intravenous immunoglobulin (IVIG), respectively. In conclusion, ABO incompatibility was the most common cause for neonatal DAT positivity. Besides the common causes of DAT positivity, there would be rare but important conditions that may lead to a positive result, such as antibodies passively acquired from mothers in the context of alloimmunizations or using drugs. In addition, as a high rate of therapeutic intervention was identified among neonates with a DAT-positive result, there is a crucial need for increasing awareness regarding early diagnosis of the condition, careful monitoring, and the employment of prenatal alloimmunization screening tests.

Transfusion Clinique et Biologique, 2021

Journal of Complementary and Integrative Medicine, 2021

Although Sanguisorba minor has been used as herbal medicine, no study has ever examined its poten... more Although Sanguisorba minor has been used as herbal medicine, no study has ever examined its potential toxicity. This study investigated acute and subacute toxicities of S. minor hydroalcoholic extract (SE). In the acute toxicity test, a single oral dose (300, 2,000, and 3,000 mg/kg) of SE was given to mice. The oral administration of SE (100, 200, and 400 mg/kg for 4 weeks) was performed to evaluate subacute toxicity. After the treatments, neurobehavioral, histopathology, hematological, and biochemical parameters were monitored. In vitro cytotoxicity was also assessed. Moreover, high-performance liquid chromatography fingerprint was done for the standardization of SE. The no-observed-adverse-effect level of SE was up to 2,000 mg/kg, and the LD50 of the prepared extract was over 3,000 mg/kg. The rats exposed to the extract did not show any marked change in their body weight. The extract at used doses did not affect neuromuscular coordination. According to the hematological, biochemic...

Iranian journal of basic medical sciences, 2019

Objectives Gentamicin belongs to the family of aminoglycoside antibiotics and is a preferred drug... more Objectives Gentamicin belongs to the family of aminoglycoside antibiotics and is a preferred drug in developing countries because of its low cost, availability, and potent effects against bacterial. However, gentamicin can induce nephrotoxicity. In this research, hydroalcoholic extract of Rheum turkestanicum was used against gentamicin- induced nephrotoxicity and its effect against gentamicin-induced nephrotoxicity in rats has been investigated. Materials and Methods The rats were placed into one of these groups: saline group, gentamicin group that received gentamicin 80 mg/kg/day for six days, and two treatment groups that received R. turkestanicum intraperitoneally at doses of 100 and 200 mg/kg body weight, respectively, 1 hr before gentamicin injections. Urine samples were collected at 24 hr to measure glucose and protein concentration. Blood samples were collected to determine serum urea and creatinine. One kidney was homogenized to measure malondialdehyde and thiol, and the oth...

The Neuroscience Journal of Shefaye Khatam, 2018

Central neuropathic pain (CNP) is considered as a complicated sensory dis turbance which many mul... more Central neuropathic pain (CNP) is considered as a complicated sensory dis turbance which many multiple sclerosis (MS) patients suffer from. Although monophasic experimental autoimmune encephalomyelitis (EAE) mouse model is a gold s tandard model in preclinical research of MS, severe movement deficit could confound pain behaviors evaluation over the disease course. In this s tudy, complete Freund’s adjuvant (CFA) was subs tituted with an acylated triterpene glycoside saponin adjuvant named quillaja saponin-21 (QS-21) to es tablish EAE model for CNP development. Twentyfour, 5-7 weeks old female C57BL/6 mice were randomly divided into three groups. Two groups immunized with MOG35-55 peptide emulsified with CFA and QS-21 adjuvant. The las t group received PBS as negative control group. Thermal hyperalgesia as a CNP clinical manifes tation through hot plate tes t and clinical signs were assessed for 60 days’ pos t immunization (p.i). On days 21 and 60 p.i mice were sacrificed and TCD4+, ...

Physiology and Pharmacology, 2021

Introduction: Lead is a heavy metal with vast usage in the industry. Lead toxicity affects any or... more Introduction: Lead is a heavy metal with vast usage in the industry. Lead toxicity affects any organ in the body. It causes various clinical presentations, which leads to diagnostic complexity. Regarding recent increased observation of cases with lead toxicity in our center, we aimed to evaluate the frequencies of lead toxicity in patients referred to Imam-Reza Hospital’s laboratory and find a possible relationship between the blood lead level (BLL) and hematological and biochemical tests. Methods: From 2016 to 2017, the patients referred to Imam-Reza hospital’s laboratory to detect BLL enrolled in the study. Among them, 254 adult cases with BLLs≥10 μg/dl were selected. Complete blood counts and peripheral blood smear were done. Other lab data were extracted from hospital files. Results: The mean BLL of 1649 participants was 59.11±116.25 μg/dl, ranging from 0 to 1580. Sixty nine percent of them had lead toxicity. Eighty-one percent (n=1341) of patients were males and 18.7% (n=308) w...

Journal of Hematopathology, 2020

Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is a rare subtype of Hodgkin lymphoma (HL... more Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is a rare subtype of Hodgkin lymphoma (HL). It shows different histoarchitectural patterns which are reported to be of prognostic significance. This study aimed to investigate the clinicopathological features of NLPHL cases in Shiraz, Iran, and their possible impact on prognosis. Between March 2014 and November 2019, all records for lymphoma were extracted from the electronic files of hospitals affiliated with Shiraz University of Medical Sciences. The slides of all NLPHL cases were extracted and reviewed. Other necessary data was retrieved from hospital records and through direct contact with patients. Among1168 lymphoma cases, 382 were HLs and 19.1% (73) of them were of the NLPHL type. The median age of NLPHL patients was 34 years. Of the patients, 51.5% showed variant patterns, and 11% of them had BM involvement. The variant morphology was significantly associated with BM involvement (p = 0.02). Patients with higher stage tumors had lower OS and PFS rates. Stage, BM involvement, and infra-diaphragmatic involvement were significantly associated with outcomes. The rate of NLPHL and variant morphology seems to be high in our center in Iran. The variant morphology was associated with a higher BM involvement rate but not with OS or PFS. Higher stage, BM, and subdiaphragmatic disease involvement were associated with worse outcomes.

Life Sciences, 2020

AIMS Increased expression of inhibitor of apoptosis (IAP) genes has been associated with progress... more AIMS Increased expression of inhibitor of apoptosis (IAP) genes has been associated with progressive cancer and chemoresistance. Accordingly, blockade of IAPs by BV6 has resulted in ameliorative outcomes. Interleukin (IL)-6 is another important mediator involved in the growth and survival of tumor cells. Therefore, we hypothesized that simultaneous inhibition of IAPs and IL-6 could be a new promising anti-tumor treatment strategy. MATERIALS AND METHODS In this study, we generated and characterized hyaluronate-PEG-Chitosan-Lactate (H-PCL) nanoparticles (NPs) to simultaneously deliver IL6-specific siRNA and BV6 to 4T1 (breast cancer) and CT26 (colon cancer) cells, and investigate the anti-tumor properties of this combination therapy both in vitro and in vivo. KEY FINDINGS H-PCL NPs exhibited good physicochemical properties leading to efficient transfection of cancer cells and suppression of target molecules. Moreover, combination therapy synergistically increased apoptosis, as well as decreased cell migration, proliferation, colony formation, and angiogenesis in both 4T1 and CT26 cell lines and suppressed cancer progression in tumor-bearing mice that was associated with enhanced survival time. SIGNIFICANCE These findings imply the effectiveness of cancer combination therapy by using H-PCL NPs loaded with anti-IL-6 siRNA and BV6.

Journal of Medicinal Plants, 2020

Journal of Hematopathology, 2020

Mantle cell lymphoma (MCL) is a relatively rare lymphoma subtype. Almost all cases show common ge... more Mantle cell lymphoma (MCL) is a relatively rare lymphoma subtype. Almost all cases show common genetic abnormality t(11;14), resulting in overexpression of cyclin D1 protein. The clinical course is usually aggressive, and the disease is mainly considered incurable but obvious clinical heterogeneity exists. Most patients experience a rapidly progressive course, and some go through an indolent course of disease. So new strategies are needed for risk stratification and may allow for a more personalized approach in the future. We retrospectively studied 50 cases of MCL which were diagnosed at a single center over about 5 years to determine clinicopathologic and immunophenotyping findings. This study focuses on (1) age, gender, and location of tumor at presentation; (2) some challenges in the diagnostic criteria; (3) cytomorphology, histomorphology, immunophenotyping, and proliferation rate; and (4) overall survival and finally relationship with demographic and pathologic findings which shows no association between survival of patients and gender, cytomorphology, histology, and location of tumor while increased mitotic activity and age are prognostically important factors. Cyclin D1 immunostaining is strictly recommended in all low-grade B cell lymphoma due to absence of CD5 expression on 20% of confirmed MCL and also on all high-grade B cell lymphoma for avoiding overlook of blastic variant of MCL.

Nephro-Urology Monthly, 2020

Background: The growing prevalence of Attention Deficit Hyperactivity Disorder (ADHD) and the non... more Background: The growing prevalence of Attention Deficit Hyperactivity Disorder (ADHD) and the non-medical use of Methylphenidate (MPH) among the youth have lead male infertility to be a major health problem. Objectives: The present study was conducted to investigate the impacts of MPH administration on different aspects of productivity, including total body weight, testis weight, spermatogenesis, sperm motility, histopathology changes, and sex hormone serum levels in male rats. Methods: This study was performed with 54 eight-week-old male rats divided into one control and two experimental groups. The experimental groups were gavaged with 2 and 10 mg/kg methylphenidate daily while the control group was gavaged with normal saline (at the same dosage). After 60 days, rats were subjected to blood sampling and bilateral orchidoepididymectomy under anesthesia. Spermogram, histological, and hormonal evaluations were performed on the samples. Testes weight and total body weight were also re...

Pharmaceutical Sciences, 2019

Background: Some plants stimulate spermatogenesis and increase fertility, while some cause sperma... more Background: Some plants stimulate spermatogenesis and increase fertility, while some cause spermatogenesis arrest. So far, the effects of bene (Pistacia atlantica) on male fertility have not been studied. The aim of this study was to investigate the effects of bene on sperm parameters, testicular histopathology, sperm quality, and oxidative stress in busulfan-induced infertile mice. Methods: Thirty-five male BALB/c mice were randomly assigned to control, sham, busulfan, bene, and bene + busulfan groups. The busulfan group received 10 mg/kg as a single dose and intraperitoneally. The bene group received pellets containing 10% of bene. Another group received 10 mg/kg busulfan and was fed with pellet containing 10% bene. Then, sperms, sperm chromatin quality, testicular histopathology, and oxidative stress levels were studied on the 35th day of the experiment. Results: Busulfan injection resulted in a significant reduction in sperm parameters compared to the control group (p<0.001);...

International Journal of Radiation Biology, 2019

Propose-CatSper protein channels are responsible for the entry of Ca2+ into sperm cells. These pr... more Propose-CatSper protein channels are responsible for the entry of Ca2+ into sperm cells. These proteins play an important role in motility and male fertility. So it is important to find out whether or not environmental factors, such as gamma radiation, have an effect on the expression of Catsper genes. In this study, we investigated the effects of gamma radiation on the expression of CatSper1 and CatSper2 genes. Materials and Methods-Twenty-one male NMRI mice were divided into three groups: a control group without gamma radiation, and two experimental groups; Group 1 treated with 1Gy of gamma radiation, and Group 2 treated with a higher dose of 2Gy gamma radiation. Testes were removed from all groups of animals 35 days following irradiation and the testicular tissue, processed and embedded in paraffin blocks for sectioning and histological examination. Sperm samples were also taken from the epididymis for microscopic. Sperm parameters such as sperm count, morphology, motility and viability rates were analysed. Expression of CatSper genes was evaluated using Real-time PCR. Data were analysed using the SPSS software and ANOVA test. Results-Our results showed that after treatment with gamma radiation, testes morphology was changed. Epididymal sperm count, motility, and morphology rates were significantly affected in both experimental groups compared to the control group. The relative expressions of CatSper 1 and 2 genes were significantly reduced in the irradiated mice (1 Gy and 2 Gy) than non-irradiated ones. conclusions-Gamma radiations not only change testes histology and sperm parameters, but also decrease the expression of CatSper 1 & 2 genes in male mice.

Multiple Sclerosis and Related Disorders, 2019

Background: The majority of patients with multiple sclerosis (MS) suffer from central neuropathic... more Background: The majority of patients with multiple sclerosis (MS) suffer from central neuropathic pain (CNP). Using experimental autoimmune encephalomyelitis (EAE) model, only a few experiments were performed to assess pain behaviors in MS. To address this issue, complete Freund's adjuvant (CFA) was replaced with an acylated triterpene glycoside saponin adjuvant named quillaja saponin-21 (QS-21) to develop CNP in the EAE mouse model. The deacylated form of QS-21, named QT-0101, has been suggested to have an immunomodulatory effect. Thus, QT-0101 was used as a vaccine adjuvant to modulate the immune system against myelin oligodendrocyte glycoprotein (MOG35-55) antigen. Methods: In this study, C57BL/6 mice, except for mice in the negative control (PBS) and MOG groups, were divided into three groups and immunized by MOG35-55 emulsified with CFA, QS-21, or QT-0101 adjuvants, respectively. Thermal hyperalgesia, as a CNP clinical manifestation, through the Hot Plate test and the clinical signs, was assessed for 60 days after immunization. On days 21 and 60, mice were sacrificed and the frequency of TCD4 + , TCD8 + , IL-17 + , IL-4 + , and CD25 + /FoxP3 + cells population in the total splenocytes population was assessed by flow cytometry. Infiltration of Leukocytes into the brain and demyelination of white matter were also evaluated by histopathologic studies. Results: Our results revealed that unlike the MOG+QT-0101 group, the MOG+QS-21 and MOG+CFA groups represented clinical symptoms that mimic the mild relapsing-remitting and monophasic models, respectively. Thermal hyperalgesia, as a CNP clinical manifestation, developed in the bilateral hind paws in the MOG+CFA and MOG+QS-21 mice groups during the onset of neurologic deficits, but it is maintained until completion of the study only in MOG+QS-21 mice group. The frequency of TCD4 + , TCD8 + and IL-17 + cells population in the MOG+QS-21 and MOG+CFA mice groups, as well as IL-4 + and CD25 + /Foxp3 + cells population in the MOG+QT-0101 mice group, significantly increased in comparison with the PBS mice group. Infiltration of inflammatory cells increased significantly in the MOG+QS-21 and MOG+CFA mice groups compared with the PBS mice group. Demyelination of white matter was identified significantly only in the MOG+CFA mice group compared with the PBS mice group. Conclusion: These results showed that QS-21 is a suitable adjuvant for the establishment of a mild relapsingremitting EAE model for CNP development and open a new avenue to future pre-clinical and clinical research studies related to CNP treatment. Nevertheless, QT-0101 seems to have the potential to act as a vaccine adjuvant with immunomodulatory property against auto-antigens.

Environmental Toxicology and Pharmacology, 2018

High light Administration with either lead acetate or mercury chloride caused degenerative damage... more High light Administration with either lead acetate or mercury chloride caused degenerative damage in seminiferous tubules and reduction in sperm quality and expression of CatSper 1, 2 genes in m It is possible, owing to structural similarities, these metals are substitutes for calcium ions and have effects on calcium channel

Toxin Reviews, 2017

This study was designed to investigate the effect of the administration of cadmium and nickel on ... more This study was designed to investigate the effect of the administration of cadmium and nickel on the histology of the testes, sperm parameters, and the expression of CatSper 1 and CatSper 2 genes in adult male mice. Despite the vital role of CatSper genes in male fertility, very little is known about the factors that regulate their expression. Thirty-two adult male mice were randomly allocated into four groups. The control group received no treatment. The sham group was injected with normal saline. The cadmium and nickel groups were injected with 2 mg/kg/ day of cadmium chloride or 5 mg/kg/day of nickel chloride for 2-weeks as models for testicular injury. Histological study and the analysis of their sperm were performed according to WHO's guidelines for the examination of human sperm. In addition, a prooxidant antioxidant balance assay and real-time PCR were performed 35 days after the treatment, as the duration of spermatogenesis cycle in mice is 35 days. The data were analyzed using SPSS software using ANOVA. Both nickel and cadmium injections caused a reduction in sperm parameters as well as a decrease in the thickness of the germinal epithelium. The administration of cadmium caused down-regulation in the expression of both CatSper1 and CatSper2 genes. Only cadmium increased the PAB values.

Journal of Basic Research in Medical Sciences, 2016

Introduction: Apoptosis is an important mechanism in both physiological and pathological conditio... more Introduction: Apoptosis is an important mechanism in both physiological and pathological conditions. The BCL2 family of proteins plays a critical role in regulation of apoptotic cell death. Up and down regulation of BCL2-like 12 (BCL2L12), a new member of the BCL2 family, has been reported in several malignancies. However, the expression level of BCL2L12 rarely has been studied in leukemia. This study was designed to investigate the mRNA expression of BCL2L12 in patients with acute leukemia. Materials and methods: 90 patients with acute leukemia as case group and 90 healthy persons as controls, were participated this study. RNA was extracted from peripheral blood samples. Expression level of BCL2L12 mRNA was evaluated by a quantitative real-time polymerase chain reaction (qRT-PCR) method and its association with clinical and laboratory findings was analyzed. Results: The expression of BCL2L12 mRNA was significantly lower in acute lymphoblastic leukemia (ALL) cases comparing the controls (P<0.001), while it was not significantly different in acute myeloid leukemia (AML) samples compared the control group. In addition, there were higher BCL2L12 level in females (than in males) and in patients with t(12;21) in ALL patients. There was no association between BCL2L12 expression level and other clinical and laboratory findings of AML patients. Conclusion: BCL2L12 seems play a role in the pathogenesis of ALL. Further studies with larger sample size is needed to clarify its probable impact on prognosis and therapeutic response.

Journal of Cell Communication and Signaling, 2016

The expression of GLI1 as a downstream gene of sonic hedgehog (Hh) pathway, studied in a variety ... more The expression of GLI1 as a downstream gene of sonic hedgehog (Hh) pathway, studied in a variety of cancers including esophageal squamous cell carcinoma (ESCC). However, the interaction of Hh with other developmental pathways needs to be elucidated. In this study, we aimed to investigate the correlation of GLI1 expression with transcription factors (TFs) of stem cell signaling pathways, and their association with clinico-pathological data of ESCC. Using real-time PCR, we assessed the expression of GLI1 mRNA in 49 ESCC patients, and analyzed the correlation between GLI1 and selected TFs. The results showed overexpression of GLI1 in ESCC tissues in significant correlation with lymph node metastasis. The GLI1 up-regulation was also correlated to the SOX2 and SIZN1 (Smad-interacting zinc finger protein) expression. These correlations may confirmed the role of GLI1 in crosstalk among different cell signaling pathways in ESCC. To our knowledge, this is the first study to demonstrate the correlation of GLI1 expression with stemness marker and BMP signaling in ESCC.

Transfusion Clinique Et Biologique, Dec 1, 2021

Biomedicine & Pharmacotherapy, 2022

Transfusion and Apheresis Science, 2022

Hemolytic disease of the fetus and newborn (HDFN) is a clinically significant problem that may po... more Hemolytic disease of the fetus and newborn (HDFN) is a clinically significant problem that may potentially affect any pregnancy. Direct antiglobulin test (DAT) is considered to be an important test in identifying newborns who are suspected to have HDN. This study aims in reviewing data regarding a positive DAT result concerning etiology and the development of HDN over a period of 10 years. A retrospective study of all neonates with a positive DAT result between January 2011 and December 2020 was performed. Data were obtained from patients' electronic hospital files, transfusion medicine databases, and medical birth records. Laboratory parameters along with clinical interventions in neonates with a DAT-positive result and a comparison group of DAT-negative neonates were performed. 36,000 deliveries were registered in this period. 176 (2.65 %) neonates had a positive DAT result. ABO-incompatibility was the most common cause with 59.1 %; Rh incompatibility 13.8 %, minor blood group incompatibility, and other RBC-related antibodies 10.1 %, and unspecified etiology in 17 % of cases. Among DAT-positive cases, 32.7 % of neonates were diagnosed with HDN. ABO-incompatibility was the major reason as well. Initial mean total bilirubin levels were higher in the DAT-positive group than the control group (p < 0.001), and these neonates also had a lower initial hemoglobin level (p < 0.001). The need for therapeutic interventions was significantly higher in DAT-positive neonates (p < 0.001) as 86.8 % underwent phototherapy, with 32.7 %, and 17.6 % receiving exchange transfusion (ET) and intravenous immunoglobulin (IVIG), respectively. In conclusion, ABO incompatibility was the most common cause for neonatal DAT positivity. Besides the common causes of DAT positivity, there would be rare but important conditions that may lead to a positive result, such as antibodies passively acquired from mothers in the context of alloimmunizations or using drugs. In addition, as a high rate of therapeutic intervention was identified among neonates with a DAT-positive result, there is a crucial need for increasing awareness regarding early diagnosis of the condition, careful monitoring, and the employment of prenatal alloimmunization screening tests.

Transfusion Clinique et Biologique, 2021

Journal of Complementary and Integrative Medicine, 2021

Although Sanguisorba minor has been used as herbal medicine, no study has ever examined its poten... more Although Sanguisorba minor has been used as herbal medicine, no study has ever examined its potential toxicity. This study investigated acute and subacute toxicities of S. minor hydroalcoholic extract (SE). In the acute toxicity test, a single oral dose (300, 2,000, and 3,000 mg/kg) of SE was given to mice. The oral administration of SE (100, 200, and 400 mg/kg for 4 weeks) was performed to evaluate subacute toxicity. After the treatments, neurobehavioral, histopathology, hematological, and biochemical parameters were monitored. In vitro cytotoxicity was also assessed. Moreover, high-performance liquid chromatography fingerprint was done for the standardization of SE. The no-observed-adverse-effect level of SE was up to 2,000 mg/kg, and the LD50 of the prepared extract was over 3,000 mg/kg. The rats exposed to the extract did not show any marked change in their body weight. The extract at used doses did not affect neuromuscular coordination. According to the hematological, biochemic...

Iranian journal of basic medical sciences, 2019

Objectives Gentamicin belongs to the family of aminoglycoside antibiotics and is a preferred drug... more Objectives Gentamicin belongs to the family of aminoglycoside antibiotics and is a preferred drug in developing countries because of its low cost, availability, and potent effects against bacterial. However, gentamicin can induce nephrotoxicity. In this research, hydroalcoholic extract of Rheum turkestanicum was used against gentamicin- induced nephrotoxicity and its effect against gentamicin-induced nephrotoxicity in rats has been investigated. Materials and Methods The rats were placed into one of these groups: saline group, gentamicin group that received gentamicin 80 mg/kg/day for six days, and two treatment groups that received R. turkestanicum intraperitoneally at doses of 100 and 200 mg/kg body weight, respectively, 1 hr before gentamicin injections. Urine samples were collected at 24 hr to measure glucose and protein concentration. Blood samples were collected to determine serum urea and creatinine. One kidney was homogenized to measure malondialdehyde and thiol, and the oth...

The Neuroscience Journal of Shefaye Khatam, 2018

Central neuropathic pain (CNP) is considered as a complicated sensory dis turbance which many mul... more Central neuropathic pain (CNP) is considered as a complicated sensory dis turbance which many multiple sclerosis (MS) patients suffer from. Although monophasic experimental autoimmune encephalomyelitis (EAE) mouse model is a gold s tandard model in preclinical research of MS, severe movement deficit could confound pain behaviors evaluation over the disease course. In this s tudy, complete Freund’s adjuvant (CFA) was subs tituted with an acylated triterpene glycoside saponin adjuvant named quillaja saponin-21 (QS-21) to es tablish EAE model for CNP development. Twentyfour, 5-7 weeks old female C57BL/6 mice were randomly divided into three groups. Two groups immunized with MOG35-55 peptide emulsified with CFA and QS-21 adjuvant. The las t group received PBS as negative control group. Thermal hyperalgesia as a CNP clinical manifes tation through hot plate tes t and clinical signs were assessed for 60 days’ pos t immunization (p.i). On days 21 and 60 p.i mice were sacrificed and TCD4+, ...

Physiology and Pharmacology, 2021

Introduction: Lead is a heavy metal with vast usage in the industry. Lead toxicity affects any or... more Introduction: Lead is a heavy metal with vast usage in the industry. Lead toxicity affects any organ in the body. It causes various clinical presentations, which leads to diagnostic complexity. Regarding recent increased observation of cases with lead toxicity in our center, we aimed to evaluate the frequencies of lead toxicity in patients referred to Imam-Reza Hospital’s laboratory and find a possible relationship between the blood lead level (BLL) and hematological and biochemical tests. Methods: From 2016 to 2017, the patients referred to Imam-Reza hospital’s laboratory to detect BLL enrolled in the study. Among them, 254 adult cases with BLLs≥10 μg/dl were selected. Complete blood counts and peripheral blood smear were done. Other lab data were extracted from hospital files. Results: The mean BLL of 1649 participants was 59.11±116.25 μg/dl, ranging from 0 to 1580. Sixty nine percent of them had lead toxicity. Eighty-one percent (n=1341) of patients were males and 18.7% (n=308) w...

Journal of Hematopathology, 2020

Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is a rare subtype of Hodgkin lymphoma (HL... more Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is a rare subtype of Hodgkin lymphoma (HL). It shows different histoarchitectural patterns which are reported to be of prognostic significance. This study aimed to investigate the clinicopathological features of NLPHL cases in Shiraz, Iran, and their possible impact on prognosis. Between March 2014 and November 2019, all records for lymphoma were extracted from the electronic files of hospitals affiliated with Shiraz University of Medical Sciences. The slides of all NLPHL cases were extracted and reviewed. Other necessary data was retrieved from hospital records and through direct contact with patients. Among1168 lymphoma cases, 382 were HLs and 19.1% (73) of them were of the NLPHL type. The median age of NLPHL patients was 34 years. Of the patients, 51.5% showed variant patterns, and 11% of them had BM involvement. The variant morphology was significantly associated with BM involvement (p = 0.02). Patients with higher stage tumors had lower OS and PFS rates. Stage, BM involvement, and infra-diaphragmatic involvement were significantly associated with outcomes. The rate of NLPHL and variant morphology seems to be high in our center in Iran. The variant morphology was associated with a higher BM involvement rate but not with OS or PFS. Higher stage, BM, and subdiaphragmatic disease involvement were associated with worse outcomes.

Life Sciences, 2020

AIMS Increased expression of inhibitor of apoptosis (IAP) genes has been associated with progress... more AIMS Increased expression of inhibitor of apoptosis (IAP) genes has been associated with progressive cancer and chemoresistance. Accordingly, blockade of IAPs by BV6 has resulted in ameliorative outcomes. Interleukin (IL)-6 is another important mediator involved in the growth and survival of tumor cells. Therefore, we hypothesized that simultaneous inhibition of IAPs and IL-6 could be a new promising anti-tumor treatment strategy. MATERIALS AND METHODS In this study, we generated and characterized hyaluronate-PEG-Chitosan-Lactate (H-PCL) nanoparticles (NPs) to simultaneously deliver IL6-specific siRNA and BV6 to 4T1 (breast cancer) and CT26 (colon cancer) cells, and investigate the anti-tumor properties of this combination therapy both in vitro and in vivo. KEY FINDINGS H-PCL NPs exhibited good physicochemical properties leading to efficient transfection of cancer cells and suppression of target molecules. Moreover, combination therapy synergistically increased apoptosis, as well as decreased cell migration, proliferation, colony formation, and angiogenesis in both 4T1 and CT26 cell lines and suppressed cancer progression in tumor-bearing mice that was associated with enhanced survival time. SIGNIFICANCE These findings imply the effectiveness of cancer combination therapy by using H-PCL NPs loaded with anti-IL-6 siRNA and BV6.

Journal of Medicinal Plants, 2020

Journal of Hematopathology, 2020

Mantle cell lymphoma (MCL) is a relatively rare lymphoma subtype. Almost all cases show common ge... more Mantle cell lymphoma (MCL) is a relatively rare lymphoma subtype. Almost all cases show common genetic abnormality t(11;14), resulting in overexpression of cyclin D1 protein. The clinical course is usually aggressive, and the disease is mainly considered incurable but obvious clinical heterogeneity exists. Most patients experience a rapidly progressive course, and some go through an indolent course of disease. So new strategies are needed for risk stratification and may allow for a more personalized approach in the future. We retrospectively studied 50 cases of MCL which were diagnosed at a single center over about 5 years to determine clinicopathologic and immunophenotyping findings. This study focuses on (1) age, gender, and location of tumor at presentation; (2) some challenges in the diagnostic criteria; (3) cytomorphology, histomorphology, immunophenotyping, and proliferation rate; and (4) overall survival and finally relationship with demographic and pathologic findings which shows no association between survival of patients and gender, cytomorphology, histology, and location of tumor while increased mitotic activity and age are prognostically important factors. Cyclin D1 immunostaining is strictly recommended in all low-grade B cell lymphoma due to absence of CD5 expression on 20% of confirmed MCL and also on all high-grade B cell lymphoma for avoiding overlook of blastic variant of MCL.

Nephro-Urology Monthly, 2020

Background: The growing prevalence of Attention Deficit Hyperactivity Disorder (ADHD) and the non... more Background: The growing prevalence of Attention Deficit Hyperactivity Disorder (ADHD) and the non-medical use of Methylphenidate (MPH) among the youth have lead male infertility to be a major health problem. Objectives: The present study was conducted to investigate the impacts of MPH administration on different aspects of productivity, including total body weight, testis weight, spermatogenesis, sperm motility, histopathology changes, and sex hormone serum levels in male rats. Methods: This study was performed with 54 eight-week-old male rats divided into one control and two experimental groups. The experimental groups were gavaged with 2 and 10 mg/kg methylphenidate daily while the control group was gavaged with normal saline (at the same dosage). After 60 days, rats were subjected to blood sampling and bilateral orchidoepididymectomy under anesthesia. Spermogram, histological, and hormonal evaluations were performed on the samples. Testes weight and total body weight were also re...

Pharmaceutical Sciences, 2019

Background: Some plants stimulate spermatogenesis and increase fertility, while some cause sperma... more Background: Some plants stimulate spermatogenesis and increase fertility, while some cause spermatogenesis arrest. So far, the effects of bene (Pistacia atlantica) on male fertility have not been studied. The aim of this study was to investigate the effects of bene on sperm parameters, testicular histopathology, sperm quality, and oxidative stress in busulfan-induced infertile mice. Methods: Thirty-five male BALB/c mice were randomly assigned to control, sham, busulfan, bene, and bene + busulfan groups. The busulfan group received 10 mg/kg as a single dose and intraperitoneally. The bene group received pellets containing 10% of bene. Another group received 10 mg/kg busulfan and was fed with pellet containing 10% bene. Then, sperms, sperm chromatin quality, testicular histopathology, and oxidative stress levels were studied on the 35th day of the experiment. Results: Busulfan injection resulted in a significant reduction in sperm parameters compared to the control group (p<0.001);...

International Journal of Radiation Biology, 2019

Propose-CatSper protein channels are responsible for the entry of Ca2+ into sperm cells. These pr... more Propose-CatSper protein channels are responsible for the entry of Ca2+ into sperm cells. These proteins play an important role in motility and male fertility. So it is important to find out whether or not environmental factors, such as gamma radiation, have an effect on the expression of Catsper genes. In this study, we investigated the effects of gamma radiation on the expression of CatSper1 and CatSper2 genes. Materials and Methods-Twenty-one male NMRI mice were divided into three groups: a control group without gamma radiation, and two experimental groups; Group 1 treated with 1Gy of gamma radiation, and Group 2 treated with a higher dose of 2Gy gamma radiation. Testes were removed from all groups of animals 35 days following irradiation and the testicular tissue, processed and embedded in paraffin blocks for sectioning and histological examination. Sperm samples were also taken from the epididymis for microscopic. Sperm parameters such as sperm count, morphology, motility and viability rates were analysed. Expression of CatSper genes was evaluated using Real-time PCR. Data were analysed using the SPSS software and ANOVA test. Results-Our results showed that after treatment with gamma radiation, testes morphology was changed. Epididymal sperm count, motility, and morphology rates were significantly affected in both experimental groups compared to the control group. The relative expressions of CatSper 1 and 2 genes were significantly reduced in the irradiated mice (1 Gy and 2 Gy) than non-irradiated ones. conclusions-Gamma radiations not only change testes histology and sperm parameters, but also decrease the expression of CatSper 1 & 2 genes in male mice.

Multiple Sclerosis and Related Disorders, 2019

Background: The majority of patients with multiple sclerosis (MS) suffer from central neuropathic... more Background: The majority of patients with multiple sclerosis (MS) suffer from central neuropathic pain (CNP). Using experimental autoimmune encephalomyelitis (EAE) model, only a few experiments were performed to assess pain behaviors in MS. To address this issue, complete Freund's adjuvant (CFA) was replaced with an acylated triterpene glycoside saponin adjuvant named quillaja saponin-21 (QS-21) to develop CNP in the EAE mouse model. The deacylated form of QS-21, named QT-0101, has been suggested to have an immunomodulatory effect. Thus, QT-0101 was used as a vaccine adjuvant to modulate the immune system against myelin oligodendrocyte glycoprotein (MOG35-55) antigen. Methods: In this study, C57BL/6 mice, except for mice in the negative control (PBS) and MOG groups, were divided into three groups and immunized by MOG35-55 emulsified with CFA, QS-21, or QT-0101 adjuvants, respectively. Thermal hyperalgesia, as a CNP clinical manifestation, through the Hot Plate test and the clinical signs, was assessed for 60 days after immunization. On days 21 and 60, mice were sacrificed and the frequency of TCD4 + , TCD8 + , IL-17 + , IL-4 + , and CD25 + /FoxP3 + cells population in the total splenocytes population was assessed by flow cytometry. Infiltration of Leukocytes into the brain and demyelination of white matter were also evaluated by histopathologic studies. Results: Our results revealed that unlike the MOG+QT-0101 group, the MOG+QS-21 and MOG+CFA groups represented clinical symptoms that mimic the mild relapsing-remitting and monophasic models, respectively. Thermal hyperalgesia, as a CNP clinical manifestation, developed in the bilateral hind paws in the MOG+CFA and MOG+QS-21 mice groups during the onset of neurologic deficits, but it is maintained until completion of the study only in MOG+QS-21 mice group. The frequency of TCD4 + , TCD8 + and IL-17 + cells population in the MOG+QS-21 and MOG+CFA mice groups, as well as IL-4 + and CD25 + /Foxp3 + cells population in the MOG+QT-0101 mice group, significantly increased in comparison with the PBS mice group. Infiltration of inflammatory cells increased significantly in the MOG+QS-21 and MOG+CFA mice groups compared with the PBS mice group. Demyelination of white matter was identified significantly only in the MOG+CFA mice group compared with the PBS mice group. Conclusion: These results showed that QS-21 is a suitable adjuvant for the establishment of a mild relapsingremitting EAE model for CNP development and open a new avenue to future pre-clinical and clinical research studies related to CNP treatment. Nevertheless, QT-0101 seems to have the potential to act as a vaccine adjuvant with immunomodulatory property against auto-antigens.

Environmental Toxicology and Pharmacology, 2018

High light Administration with either lead acetate or mercury chloride caused degenerative damage... more High light Administration with either lead acetate or mercury chloride caused degenerative damage in seminiferous tubules and reduction in sperm quality and expression of CatSper 1, 2 genes in m It is possible, owing to structural similarities, these metals are substitutes for calcium ions and have effects on calcium channel

Toxin Reviews, 2017

This study was designed to investigate the effect of the administration of cadmium and nickel on ... more This study was designed to investigate the effect of the administration of cadmium and nickel on the histology of the testes, sperm parameters, and the expression of CatSper 1 and CatSper 2 genes in adult male mice. Despite the vital role of CatSper genes in male fertility, very little is known about the factors that regulate their expression. Thirty-two adult male mice were randomly allocated into four groups. The control group received no treatment. The sham group was injected with normal saline. The cadmium and nickel groups were injected with 2 mg/kg/ day of cadmium chloride or 5 mg/kg/day of nickel chloride for 2-weeks as models for testicular injury. Histological study and the analysis of their sperm were performed according to WHO's guidelines for the examination of human sperm. In addition, a prooxidant antioxidant balance assay and real-time PCR were performed 35 days after the treatment, as the duration of spermatogenesis cycle in mice is 35 days. The data were analyzed using SPSS software using ANOVA. Both nickel and cadmium injections caused a reduction in sperm parameters as well as a decrease in the thickness of the germinal epithelium. The administration of cadmium caused down-regulation in the expression of both CatSper1 and CatSper2 genes. Only cadmium increased the PAB values.

Journal of Basic Research in Medical Sciences, 2016

Introduction: Apoptosis is an important mechanism in both physiological and pathological conditio... more Introduction: Apoptosis is an important mechanism in both physiological and pathological conditions. The BCL2 family of proteins plays a critical role in regulation of apoptotic cell death. Up and down regulation of BCL2-like 12 (BCL2L12), a new member of the BCL2 family, has been reported in several malignancies. However, the expression level of BCL2L12 rarely has been studied in leukemia. This study was designed to investigate the mRNA expression of BCL2L12 in patients with acute leukemia. Materials and methods: 90 patients with acute leukemia as case group and 90 healthy persons as controls, were participated this study. RNA was extracted from peripheral blood samples. Expression level of BCL2L12 mRNA was evaluated by a quantitative real-time polymerase chain reaction (qRT-PCR) method and its association with clinical and laboratory findings was analyzed. Results: The expression of BCL2L12 mRNA was significantly lower in acute lymphoblastic leukemia (ALL) cases comparing the controls (P<0.001), while it was not significantly different in acute myeloid leukemia (AML) samples compared the control group. In addition, there were higher BCL2L12 level in females (than in males) and in patients with t(12;21) in ALL patients. There was no association between BCL2L12 expression level and other clinical and laboratory findings of AML patients. Conclusion: BCL2L12 seems play a role in the pathogenesis of ALL. Further studies with larger sample size is needed to clarify its probable impact on prognosis and therapeutic response.

Journal of Cell Communication and Signaling, 2016

The expression of GLI1 as a downstream gene of sonic hedgehog (Hh) pathway, studied in a variety ... more The expression of GLI1 as a downstream gene of sonic hedgehog (Hh) pathway, studied in a variety of cancers including esophageal squamous cell carcinoma (ESCC). However, the interaction of Hh with other developmental pathways needs to be elucidated. In this study, we aimed to investigate the correlation of GLI1 expression with transcription factors (TFs) of stem cell signaling pathways, and their association with clinico-pathological data of ESCC. Using real-time PCR, we assessed the expression of GLI1 mRNA in 49 ESCC patients, and analyzed the correlation between GLI1 and selected TFs. The results showed overexpression of GLI1 in ESCC tissues in significant correlation with lymph node metastasis. The GLI1 up-regulation was also correlated to the SOX2 and SIZN1 (Smad-interacting zinc finger protein) expression. These correlations may confirmed the role of GLI1 in crosstalk among different cell signaling pathways in ESCC. To our knowledge, this is the first study to demonstrate the correlation of GLI1 expression with stemness marker and BMP signaling in ESCC.