asha singh | Indian Council of Agricultural Research (ICAR) (original) (raw)
Papers by asha singh
Biomass Conversion and Biorefinery
The textile industries discharge around 10-15% dyes into effluents, posing threat to human health... more The textile industries discharge around 10-15% dyes into effluents, posing threat to human health and environment. White-rot fungi are promising candidates for elimination of variety of dyes, owing to their potential for degradation and mineralization of a broad spectrum of highly toxic, recalcitrant, and organic pollutants. In this study, different species of Pleurotus were evaluated for ligninolytic enzymes production using paddy straw, sugarcane bagasse and wheat straw for 7, 14, 21 and 28 days of incubation, respectively. Among substrates, paddy straw exhibited maximum activity of laccase (Lac) (45968.9 ± 347.2 U/g) and manganese peroxidase (MnP) (16312.1 ± 99.9 U/g) from P. ostreatus D-66 after 28 days, however the lignin peroxidase (LiP) (10823.2 ± 94.5 U/g) activity from P. pulmonarius D-79 following 21 days.
Journal of Basic Microbiology, 2019
Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic ... more Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic biomass, which contains approximately 57-74.3% of holocellulose fraction. This study was aimed at utilizing SMS of Pleurotus florida for recovery of lignocellulolytic enzymes and sugars and also as a substrate for production of cellulolytic enzymes using different isolates of Trichoderma and Aspergillus under solid-state fermentation (SSF). SMS of P. florida extracts contained significant amounts of laccase (3,015.8 ± 29.5 U/g SMS) and xylanase (1,187.9 ± 12 U/g SMS) activity. Crystallinity pattern and chemical changes in SMS revealed that SMS had a lower crystallinity index (34.2%) as compared with the raw biomass (37.8%), which, in turn, helps in enhancing the accessibility of cellulolytic enzymes to holocellulose. Among the isolates, Trichoderma longibrachiatum A-01 showed maximum activity of endoglucanase (220.4 ± 5.9 U/mg), exoglucanase (78.5 ± 3.2 U/mg) and xylanase (1,550.4 ± 11.6 U/mg) while Aspergillus aculeatus C-08 showed maximum activity of cellobiase (113.9 ± 3.9 U/mg). Extraction with sodium citrate buffer (pH 4.8) showed maximum cellulolytic enzyme activity as compared with other solvents tested. Partial purification of endoglucanase, exoglucanase, xylanase, and cellobiase resulted in 56.3% (1,112.5 U/mg), 48.4% (212.5 U/mg), 44% (4,492.3 U/mg), and 62% (705.0 U/mg) yield with an increase by 5.2-, 4.5-, 4.1-, and 5.0-fold as compared with crude extract. The results reveal that SMS from P. florida could be a potential and cost-effective substrate for production of cellulolytic enzymes from T. longibrachiatum A-01 and A. aculeatus C-08. K E Y W O R D S lignocellulolytic enzymes, Pleurotus florida, solid-state fermentation, spent mushroom substrate 1 | INTRODUCTION Mushroom cultivation is one of the largest industries involving solid-state fermentation (SSF) at the global level [1]. Currently, mushroom production is growing at a rate of 7% per annum, and it is projected to grow further in the near future. During the year 2017, 10 million tonnes of mushrooms were produced globally with major
Springer Nature , 2023
The textile industries discharge around 10-15% dyes into effluents, posing threat to human health... more The textile industries discharge around 10-15% dyes into effluents, posing threat to human health and environment. White-rot fungi are promising candidates for elimination of variety of dyes, owing to their potential for degradation and mineralization of a broad spectrum of highly toxic, recalcitrant, and organic pollutants. In this study, different species of Pleurotus were evaluated for ligninolytic enzymes production using paddy straw, sugarcane bagasse and wheat straw for 7, 14, 21 and 28 days of incubation, respectively. Among substrates, paddy straw exhibited maximum activity of laccase (Lac) (45968.9 ± 347.2 U/g) and manganese peroxidase (MnP) (16312.1 ± 99.9 U/g) from P. ostreatus D-66 after 28 days, however the lignin peroxidase (LiP) (10823.2 ± 94.5 U/g) activity from P. pulmonarius D-79 following 21 days.
south asian journal of experimental biology, 2013
Diabetes is a proven predisposing factor for vulvovaginal candidiasis, along with pregnancy, use ... more Diabetes is a proven predisposing factor for vulvovaginal candidiasis, along with pregnancy, use of broad-spectrum antibiotics, oral contraceptive, obesity etc. For the treatment of candidiasis, imidazole drugs are effective; however , due to the intrinsic or acquired resistance of Candida species to these imidazoles, and their side effects, infections by Candida species may necessitate alternative treatment. The present study was undertaken to evaluate the effect of some probiotic Lactobacilli from commercial products against Candida species isolated from vagina of 55 diabetic women. Samples were processed on Sabouraud dextrose agar media. After incubation for 24h at 37 0 C, the yeast like colonies were characterized and identified with gram staining, standard carbohydrate assimilation test and growth in differential culture media Hi-chrom agar. Four Candida species were isolated viz. Can-dida krusei, Candida albicans, Candida glabrata and Candida tropicalis. Out of them the C.krusei (33%) was prevalent in asymptomatic patients, while C.albicanse (48%) in symptomatic vaginal infection of diabetic patients. Pro-biotic Lactobacillus species were isolated from commercially available products like Bion, Ecobion, Powell and curd and identified by morphological and biochemical test. Antifungal activity of probiotics Lactobacillus species isolated from curd shown with the mean zone of diameter (mm) of inhibition and the SD was found to be 23.21±5.74 against Candida species. Therefore, it is visualized that probiotics can be used as a treatment or co-treatment of vul-vovaginal candidiasis of diabetic women and improves their life value.
asha singh rajavat, 2019
Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic ... more Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic biomass, which contains approximately 57-74.3% of holocellulose fraction. This study was aimed at utilizing SMS of Pleurotus florida for recovery of lignocellulolytic enzymes and sugars and also as a substrate for production of cellulolytic enzymes using different isolates of Trichoderma and Aspergillus under solid-state fermentation (SSF). SMS of P. florida extracts contained significant amounts of laccase (3,015.8 ± 29.5 U/g SMS) and xylanase (1,187.9 ± 12 U/g SMS) activity. Crystallinity pattern and chemical changes in SMS revealed that SMS had a lower crystallinity index (34.2%) as compared with the raw biomass (37.8%), which, in turn, helps in enhancing the accessibility of cellulolytic enzymes to holocellulose. Among the isolates, Trichoderma longibrachiatum A-01 showed maximum activity of endoglucanase (220.4 ± 5.9 U/mg), exoglucanase (78.5 ± 3.2 U/mg) and xyla-nase (1,550.4 ± 11.6 U/mg) while Aspergillus aculeatus C-08 showed maximum activity of cellobiase (113.9 ± 3.9 U/mg). Extraction with sodium citrate buffer (pH 4.8) showed maximum cellulolytic enzyme activity as compared with other solvents tested. Partial purification of endoglucanase, exoglucanase, xylanase, and cellobiase resulted in 56.3% (1,112.5 U/mg), 48.4% (212.5 U/mg), 44% (4,492.3 U/mg), and 62% (705.0 U/mg) yield with an increase by 5.2-, 4.5-, 4.1-, and 5.0-fold as compared with crude extract. The results reveal that SMS from P. florida could be a potential and cost-effective substrate for production of cellulolytic enzymes from T. longibrachiatum A-01 and A. aculeatus C-08. K E Y W O R D S lignocellulolytic enzymes, Pleurotus florida, solid-state fermentation, spent mushroom substrate
Diabetes is a proven predisposing factor for vulvovaginal candidiasis, along with pregnancy, use ... more Diabetes is a proven predisposing factor for vulvovaginal candidiasis, along with pregnancy, use of broad-spectrum antibiotics, oral contraceptive, obesity etc. For the treatment of candidiasis, imidazole drugs are effective; however , due to the intrinsic or acquired resistance of Candida species to these imidazoles, and their side effects, infections by Candida species may necessitate alternative treatment. The present study was undertaken to evaluate the effect of some probiotic Lactobacilli from commercial products against Candida species isolated from vagina of 55 diabetic women. Samples were processed on Sabouraud dextrose agar media. After incubation for 24h at 37 0 C, the yeast like colonies were characterized and identified with gram staining, standard carbohydrate assimilation test and growth in differential culture media Hi-chrom agar. Four Candida species were isolated viz. Can-dida krusei, Candida albicans, Candida glabrata and Candida tropicalis. Out of them the C.krusei (33%) was prevalent in asymptomatic patients, while C.albicanse (48%) in symptomatic vaginal infection of diabetic patients. Pro-biotic Lactobacillus species were isolated from commercially available products like Bion, Ecobion, Powell and curd and identified by morphological and biochemical test. Antifungal activity of probiotics Lactobacillus species isolated from curd shown with the mean zone of diameter (mm) of inhibition and the SD was found to be 23.21±5.74 against Candida species. Therefore, it is visualized that probiotics can be used as a treatment or co-treatment of vul-vovaginal candidiasis of diabetic women and improves their life value.
Books by asha singh
Biomass Conversion and Biorefinery
The textile industries discharge around 10-15% dyes into effluents, posing threat to human health... more The textile industries discharge around 10-15% dyes into effluents, posing threat to human health and environment. White-rot fungi are promising candidates for elimination of variety of dyes, owing to their potential for degradation and mineralization of a broad spectrum of highly toxic, recalcitrant, and organic pollutants. In this study, different species of Pleurotus were evaluated for ligninolytic enzymes production using paddy straw, sugarcane bagasse and wheat straw for 7, 14, 21 and 28 days of incubation, respectively. Among substrates, paddy straw exhibited maximum activity of laccase (Lac) (45968.9 ± 347.2 U/g) and manganese peroxidase (MnP) (16312.1 ± 99.9 U/g) from P. ostreatus D-66 after 28 days, however the lignin peroxidase (LiP) (10823.2 ± 94.5 U/g) activity from P. pulmonarius D-79 following 21 days.
Journal of Basic Microbiology, 2019
Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic ... more Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic biomass, which contains approximately 57-74.3% of holocellulose fraction. This study was aimed at utilizing SMS of Pleurotus florida for recovery of lignocellulolytic enzymes and sugars and also as a substrate for production of cellulolytic enzymes using different isolates of Trichoderma and Aspergillus under solid-state fermentation (SSF). SMS of P. florida extracts contained significant amounts of laccase (3,015.8 ± 29.5 U/g SMS) and xylanase (1,187.9 ± 12 U/g SMS) activity. Crystallinity pattern and chemical changes in SMS revealed that SMS had a lower crystallinity index (34.2%) as compared with the raw biomass (37.8%), which, in turn, helps in enhancing the accessibility of cellulolytic enzymes to holocellulose. Among the isolates, Trichoderma longibrachiatum A-01 showed maximum activity of endoglucanase (220.4 ± 5.9 U/mg), exoglucanase (78.5 ± 3.2 U/mg) and xylanase (1,550.4 ± 11.6 U/mg) while Aspergillus aculeatus C-08 showed maximum activity of cellobiase (113.9 ± 3.9 U/mg). Extraction with sodium citrate buffer (pH 4.8) showed maximum cellulolytic enzyme activity as compared with other solvents tested. Partial purification of endoglucanase, exoglucanase, xylanase, and cellobiase resulted in 56.3% (1,112.5 U/mg), 48.4% (212.5 U/mg), 44% (4,492.3 U/mg), and 62% (705.0 U/mg) yield with an increase by 5.2-, 4.5-, 4.1-, and 5.0-fold as compared with crude extract. The results reveal that SMS from P. florida could be a potential and cost-effective substrate for production of cellulolytic enzymes from T. longibrachiatum A-01 and A. aculeatus C-08. K E Y W O R D S lignocellulolytic enzymes, Pleurotus florida, solid-state fermentation, spent mushroom substrate 1 | INTRODUCTION Mushroom cultivation is one of the largest industries involving solid-state fermentation (SSF) at the global level [1]. Currently, mushroom production is growing at a rate of 7% per annum, and it is projected to grow further in the near future. During the year 2017, 10 million tonnes of mushrooms were produced globally with major
Springer Nature , 2023
The textile industries discharge around 10-15% dyes into effluents, posing threat to human health... more The textile industries discharge around 10-15% dyes into effluents, posing threat to human health and environment. White-rot fungi are promising candidates for elimination of variety of dyes, owing to their potential for degradation and mineralization of a broad spectrum of highly toxic, recalcitrant, and organic pollutants. In this study, different species of Pleurotus were evaluated for ligninolytic enzymes production using paddy straw, sugarcane bagasse and wheat straw for 7, 14, 21 and 28 days of incubation, respectively. Among substrates, paddy straw exhibited maximum activity of laccase (Lac) (45968.9 ± 347.2 U/g) and manganese peroxidase (MnP) (16312.1 ± 99.9 U/g) from P. ostreatus D-66 after 28 days, however the lignin peroxidase (LiP) (10823.2 ± 94.5 U/g) activity from P. pulmonarius D-79 following 21 days.
south asian journal of experimental biology, 2013
Diabetes is a proven predisposing factor for vulvovaginal candidiasis, along with pregnancy, use ... more Diabetes is a proven predisposing factor for vulvovaginal candidiasis, along with pregnancy, use of broad-spectrum antibiotics, oral contraceptive, obesity etc. For the treatment of candidiasis, imidazole drugs are effective; however , due to the intrinsic or acquired resistance of Candida species to these imidazoles, and their side effects, infections by Candida species may necessitate alternative treatment. The present study was undertaken to evaluate the effect of some probiotic Lactobacilli from commercial products against Candida species isolated from vagina of 55 diabetic women. Samples were processed on Sabouraud dextrose agar media. After incubation for 24h at 37 0 C, the yeast like colonies were characterized and identified with gram staining, standard carbohydrate assimilation test and growth in differential culture media Hi-chrom agar. Four Candida species were isolated viz. Can-dida krusei, Candida albicans, Candida glabrata and Candida tropicalis. Out of them the C.krusei (33%) was prevalent in asymptomatic patients, while C.albicanse (48%) in symptomatic vaginal infection of diabetic patients. Pro-biotic Lactobacillus species were isolated from commercially available products like Bion, Ecobion, Powell and curd and identified by morphological and biochemical test. Antifungal activity of probiotics Lactobacillus species isolated from curd shown with the mean zone of diameter (mm) of inhibition and the SD was found to be 23.21±5.74 against Candida species. Therefore, it is visualized that probiotics can be used as a treatment or co-treatment of vul-vovaginal candidiasis of diabetic women and improves their life value.
asha singh rajavat, 2019
Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic ... more Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic biomass, which contains approximately 57-74.3% of holocellulose fraction. This study was aimed at utilizing SMS of Pleurotus florida for recovery of lignocellulolytic enzymes and sugars and also as a substrate for production of cellulolytic enzymes using different isolates of Trichoderma and Aspergillus under solid-state fermentation (SSF). SMS of P. florida extracts contained significant amounts of laccase (3,015.8 ± 29.5 U/g SMS) and xylanase (1,187.9 ± 12 U/g SMS) activity. Crystallinity pattern and chemical changes in SMS revealed that SMS had a lower crystallinity index (34.2%) as compared with the raw biomass (37.8%), which, in turn, helps in enhancing the accessibility of cellulolytic enzymes to holocellulose. Among the isolates, Trichoderma longibrachiatum A-01 showed maximum activity of endoglucanase (220.4 ± 5.9 U/mg), exoglucanase (78.5 ± 3.2 U/mg) and xyla-nase (1,550.4 ± 11.6 U/mg) while Aspergillus aculeatus C-08 showed maximum activity of cellobiase (113.9 ± 3.9 U/mg). Extraction with sodium citrate buffer (pH 4.8) showed maximum cellulolytic enzyme activity as compared with other solvents tested. Partial purification of endoglucanase, exoglucanase, xylanase, and cellobiase resulted in 56.3% (1,112.5 U/mg), 48.4% (212.5 U/mg), 44% (4,492.3 U/mg), and 62% (705.0 U/mg) yield with an increase by 5.2-, 4.5-, 4.1-, and 5.0-fold as compared with crude extract. The results reveal that SMS from P. florida could be a potential and cost-effective substrate for production of cellulolytic enzymes from T. longibrachiatum A-01 and A. aculeatus C-08. K E Y W O R D S lignocellulolytic enzymes, Pleurotus florida, solid-state fermentation, spent mushroom substrate
Diabetes is a proven predisposing factor for vulvovaginal candidiasis, along with pregnancy, use ... more Diabetes is a proven predisposing factor for vulvovaginal candidiasis, along with pregnancy, use of broad-spectrum antibiotics, oral contraceptive, obesity etc. For the treatment of candidiasis, imidazole drugs are effective; however , due to the intrinsic or acquired resistance of Candida species to these imidazoles, and their side effects, infections by Candida species may necessitate alternative treatment. The present study was undertaken to evaluate the effect of some probiotic Lactobacilli from commercial products against Candida species isolated from vagina of 55 diabetic women. Samples were processed on Sabouraud dextrose agar media. After incubation for 24h at 37 0 C, the yeast like colonies were characterized and identified with gram staining, standard carbohydrate assimilation test and growth in differential culture media Hi-chrom agar. Four Candida species were isolated viz. Can-dida krusei, Candida albicans, Candida glabrata and Candida tropicalis. Out of them the C.krusei (33%) was prevalent in asymptomatic patients, while C.albicanse (48%) in symptomatic vaginal infection of diabetic patients. Pro-biotic Lactobacillus species were isolated from commercially available products like Bion, Ecobion, Powell and curd and identified by morphological and biochemical test. Antifungal activity of probiotics Lactobacillus species isolated from curd shown with the mean zone of diameter (mm) of inhibition and the SD was found to be 23.21±5.74 against Candida species. Therefore, it is visualized that probiotics can be used as a treatment or co-treatment of vul-vovaginal candidiasis of diabetic women and improves their life value.