Mohammed Nafi | AL-Neelain University (original) (raw)

Papers by Mohammed Nafi

Research paper thumbnail of Seroprevalence of Helicobacter pylori among elevated HA1c and Type - 2 Diabetes Mellitus Patients attending Khartoum Hospitals

Background: There are conflicting reports on Helicobacter pylori prevalence and its relationship ... more Background: There are conflicting reports on Helicobacter pylori prevalence and its relationship to patients with diabetes mellitus type 2 (T2DM). Aim: The current study was carried out to detect the sero-frequency of Helicobacter pylori among high HA1c and Type-2 diabetes mellitus patients in Khartoum. Methods: A total number of 50 plasma samples were collected randomly from uncontrolled DM patient's attending different hospitals in Khartoum, Sudan from January to March 2016 and 40 plasma samples were collected from healthy individuals as controls. The detection of Helicobacter pylori IgG was performed by using an Enzyme–linked immune-sorbent assay (ELISA). Results: Helicobacter pylori antibodies were detected in 24.0% HAc1 and Type-2 diabetes mellitus patient's and 47.5% control group (P value 0.02). Conclusion: Helicobacter pylori infection is not associated significantly with high HAc1and Type2 diabetes mellitus.

Research paper thumbnail of The Frequency Rate of vanB Gene among Vancomycin-resistant Enterococci isolated from Different Clinical Specimens in Khartoum (Sudan

Background: Glycopeptides such as vancomycin are frequently the antibiotics of choice for the

Research paper thumbnail of Seroprevalence of Herpes Simplex Virus (HSV-1 and HSV-2) Infections in Sudanese Renal Transplant Recipients

Research paper thumbnail of Sero-prevalence of hepatitis B virus (HBsAg) and hepatitis C virus (Anti-HCV) among HIV- infected in Sudan

Background: Human immunodeficiency virus (HIV), hepatitis B virus (HBV) and hepatitis C virus (HC... more Background: Human immunodeficiency virus (HIV), hepatitis B virus (HBV) and hepatitis C virus (HCV) were three most common chronic viral infections all over the world, they shares similar routes of transmission. Hepatitis coinfection with HIV is associated with increased serious life threatening complication, morbidity and mortality. Aim: To determine the seroprevalence of HBV (HBsAg) and HCV (Anti-HCV) co-infection in HIV-positive patients and to detect the shared and significant factors in the co-infection. Methods: This cross sectional study, 88 blood samples were collected from confirmed HIV positive patients (ELISA and Western blot), 52 (59.1%) and 36 (40.9%) of them were males and females, respectively; All samples were tested for HBV (HBsAg) and HCV (Anti-HCV) by Enzyme Linked Immunosorbent Assay (ELISA). Results: Among HIV infected patients HBV (HBsAg) infection were detected in 11.4% and HCV (anti-HCV) were detected in 5.7% respectively.

Research paper thumbnail of Seroprevalence of Helicobacter pylori among elevated HA1c and Type -2 Diabetes Mellitus Patients attending Khartoum Hospitals

Background: There are conflicting reports on Helicobacter pylori prevalence and its relationship ... more Background: There are conflicting reports on Helicobacter pylori prevalence and its relationship to patients with diabetes mellitus type-2 (T2DM). Aim: The current study was carried out to detect the sero-frequency of Helicobacter pylori among

Research paper thumbnail of Sero-frequency of Hepatitis B Infection among Health Care Workers in Khartoum

Background: Health care workers (HCWs) are at a great risk of occupational exposure with

Research paper thumbnail of Sensitivity and Specificity of Rapid Antigen Test in Diagnosis of Streptococcal Upper Respiratory Tract Infection

Background: Sore throat is one of the most common reasons for visits to family physicians.

Research paper thumbnail of Panton-Valentine leukocidin gene Association with Methicillin-resistant and sensitive Staphylococcus aureus strains isolated from Community and Hospital-acquired wound infections in Khartoum-Sudan

BACKGROUND: Panton-Valentine leukocidin (PVL) genes encode a potent toxin in

Research paper thumbnail of Panton-Valentine Leukocidin Gene among Hospital Acquired and Community Acquired Methicillin Resistant Staphylococcus aureus Isolates from Patients in Khartoum-Sudan

Background: Panton-Valentine Leukocidin (PVL) genes encode a highly potent toxin involved in seve... more Background: Panton-Valentine Leukocidin (PVL) genes encode a highly potent toxin involved in severe skin infections and necrotizing pneumonia, even in healthy individuals. Infections with PVL producing Staphylococcu aureus strains have increased over the last decade and attracted the attention twards the detection of the PVL toxin.

Research paper thumbnail of PREVALENCE OF HUMAN IMMUNODEFICIENCY VIRUS INFECTION AMONG TUBERCULOSIS PATIENTS IN OMDURMAN -SUDAN

Research paper thumbnail of PREVALENCE OF HERPES SIMPLEX VIRUS 1/2 AND HUMAN CYTOMEGALOVIRUS IN SUDANESE SCHIZOPHRENIC PATIENTS

Background: Cognitive impairment in the form of decreased working memory and executive functions ... more Background: Cognitive impairment in the form of decreased working memory and executive functions has been recognized as a key deficit in schizophrenia. Neurotropic viruses such as Herpes virus and human cytomegalovirus have been associated with focal gray matter deficits in patients with schizophrenia. Aim: The study was carried out to detect the prevalence and relationship of herpes simplex virus (HSV 1/2) and human cytomegalovirus (HCMV) among Sudanese schizophrenia patients. Methods: A total number of 60 plasma samples were collected randomly from schizophrenia patients at Kubar hospital in Khartoum, Sudan. On the other hand, 30 samples were collected from healthy individuals were served as controls. The detection of HSV1/2 and HCMV IgG antibodies were performed by using an Enzyme-linked immunosorbent assay (ELISA). The results: HSV1/2 IgG antibodies were detected in 95% schizophrenic patients and 93.3% control (0.75 P value), while IgG antibodies to HCMV were detected in 96.7% schizophrenic patients and (90.0%) control (P value 0.19).

Research paper thumbnail of Prevalence and Antimicrobial Susceptibility Pattern of Methicillin Resistance Staphylococcus aureus in Khartoum Sudan

Methicillin-resistant Staphylococcus aureus (MRSA) has become increasingly prevalent worldwide. M... more Methicillin-resistant Staphylococcus aureus (MRSA) has become increasingly prevalent worldwide. Multi-resistant MRSA has been commonly reported to be one of the commonest causes of nosocomial infections worldwide. Also, recent reports describe MRSA in community. The aim of this study was to determine the prevalence of MRSA and their antimicrobial susceptibility pattern among hospital and community acquired Staphylococcus aureus isolates from Khartoum hospitals (Sudan), from September 2010 to September 2011. This is a cross sectional study. Staphylococcus aureus strains were isolated and identified from patients suffering from skin and wound infections in Khartoum using conventional microbiology techniques. MRSA strains were investigated by detection of mecA gene using PCR. MRSA strains were also tested for antimicrobial resistance using Kirby-Bauer NCCLS modified disc diffusion technique. A total of 223 strains of Staphylococcus aureus isolates were collected from 400 patients suffering wound and skin infections 46.2% (103/223) strains were from community acquired infections, and 53.8% (120/223) strains were from hospital acquired infections, 78 out of 223 (35.3%) were found to be

Research paper thumbnail of Laboratory medicine journal Prevalence and Antimicrobial Susceptibility Pattern of Extended Spectrum β-Lactamases Producing Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis in Khartoum Sudan

Background: Resistance to broad-spectrum β-lactams, mediated by extended-spectrum β lactamase enz... more Background: Resistance to broad-spectrum β-lactams, mediated by extended-spectrum β lactamase enzymes (ESBL), has become increasingly prevalent worldwide. Objective: The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of extended spectrum β-lactamases producing Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis isolated from clinical specimens. Methods: This is across sectional study. A total of

Research paper thumbnail of PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF BACTERIA CAUSING POSTOPERATIVE WOUND INFECTIONS IN PORT-SUDAN

Research paper thumbnail of FREQUENCY OF HEPATITIS E VIRUS AMONG RENAL TRANSPLANT RECIPIENTS PATIENTS IN KHARTOUM -SUDAN

Research paper thumbnail of Frequency of Hepatitis E Virus among Pregnant Women Attending Khartoum Hospitals

Background: Hepatitis E virus (HEV) causes large-scale epidemics of acute viral hepatitis, partic... more Background: Hepatitis E virus (HEV) causes large-scale epidemics of acute viral hepatitis, particularly in developing countries with high mortality rate among pregnant women. The infection is more severe, often leading to fulminant hepatic failure and death in a significant proportion of patients. Objective: The aim of this study was to verify the frequency of Hepatitis E virus among pregnant women attending Khartoum hospitals. Methods: Enzyme linked immunoassay (ELISA) was done to determine the presence of anti-HEV IgG among 90 pregnant women during the period from July to September 2013. The results: HEV IgG antibodies were detected in 41.1% (37/90). The highest percentages were recorded in the second and third trimesters of pregnancies (37.8% and 48.7%) respectively. Conclusion: This study found a high frequency of anti-HEV IgG among Sudanese pregnant women in Khartoum.

Research paper thumbnail of Laboratory medicine journal Detection of Carbapenem-Resistant Escherichia coli, Klebsiellapneumoniae and Proteus mirabilis clinical isolates in Khartoum; Sudan

Background: Carbapenem resistantGram-negativerods are a group of emerging, highly drug

Research paper thumbnail of COMPARISON OF TYPHIDOT-EIA AND WIDAL TEST IN RESPECT TO POLYMERASE CHAIN REACTION AS DIAGNOSTIC PROCEDURES FOR EARLY DIAGNOSIS OF TYPHOID FEVER

Research paper thumbnail of Antimicrobial Resistance of Escherichia coli Strains isolated from Community and Hospital-acquired Urinary Tract Infections in Khartoum (Sudan

Background: Urinary tract infections (UTIs) are among the most common indications for

Research paper thumbnail of VANCOMYCIN RESISTANCE AMONG METHICILLIN RESISTANT Staphylococcus aureus ISOLATES IN KHARTOUM-SUDAN

Background: Glycopeptides such as vancomycin are frequently the antibiotics of choice for the tre... more Background: Glycopeptides such as vancomycin are frequently the antibiotics of choice for the treatment of infections caused by methicillin resistant Staphylococcus aureus. For the last 10 years incidence of vancomycin intermediate S. aureus and vancomycin resistant S. aureus has been increasing in various parts of the world Objective: The aim of this study was to determine the prevalence of vancomycin intermediate S. aureus and vancomycin resistant S. aureus and their antimicrobial susceptibility pattern among hospital and community acquired Methicillin Resistant Staphylococcus aureus isolates.

Research paper thumbnail of Seroprevalence of Helicobacter pylori among elevated HA1c and Type - 2 Diabetes Mellitus Patients attending Khartoum Hospitals

Background: There are conflicting reports on Helicobacter pylori prevalence and its relationship ... more Background: There are conflicting reports on Helicobacter pylori prevalence and its relationship to patients with diabetes mellitus type 2 (T2DM). Aim: The current study was carried out to detect the sero-frequency of Helicobacter pylori among high HA1c and Type-2 diabetes mellitus patients in Khartoum. Methods: A total number of 50 plasma samples were collected randomly from uncontrolled DM patient's attending different hospitals in Khartoum, Sudan from January to March 2016 and 40 plasma samples were collected from healthy individuals as controls. The detection of Helicobacter pylori IgG was performed by using an Enzyme–linked immune-sorbent assay (ELISA). Results: Helicobacter pylori antibodies were detected in 24.0% HAc1 and Type-2 diabetes mellitus patient's and 47.5% control group (P value 0.02). Conclusion: Helicobacter pylori infection is not associated significantly with high HAc1and Type2 diabetes mellitus.

Research paper thumbnail of The Frequency Rate of vanB Gene among Vancomycin-resistant Enterococci isolated from Different Clinical Specimens in Khartoum (Sudan

Background: Glycopeptides such as vancomycin are frequently the antibiotics of choice for the

Research paper thumbnail of Seroprevalence of Herpes Simplex Virus (HSV-1 and HSV-2) Infections in Sudanese Renal Transplant Recipients

Research paper thumbnail of Sero-prevalence of hepatitis B virus (HBsAg) and hepatitis C virus (Anti-HCV) among HIV- infected in Sudan

Background: Human immunodeficiency virus (HIV), hepatitis B virus (HBV) and hepatitis C virus (HC... more Background: Human immunodeficiency virus (HIV), hepatitis B virus (HBV) and hepatitis C virus (HCV) were three most common chronic viral infections all over the world, they shares similar routes of transmission. Hepatitis coinfection with HIV is associated with increased serious life threatening complication, morbidity and mortality. Aim: To determine the seroprevalence of HBV (HBsAg) and HCV (Anti-HCV) co-infection in HIV-positive patients and to detect the shared and significant factors in the co-infection. Methods: This cross sectional study, 88 blood samples were collected from confirmed HIV positive patients (ELISA and Western blot), 52 (59.1%) and 36 (40.9%) of them were males and females, respectively; All samples were tested for HBV (HBsAg) and HCV (Anti-HCV) by Enzyme Linked Immunosorbent Assay (ELISA). Results: Among HIV infected patients HBV (HBsAg) infection were detected in 11.4% and HCV (anti-HCV) were detected in 5.7% respectively.

Research paper thumbnail of Seroprevalence of Helicobacter pylori among elevated HA1c and Type -2 Diabetes Mellitus Patients attending Khartoum Hospitals

Background: There are conflicting reports on Helicobacter pylori prevalence and its relationship ... more Background: There are conflicting reports on Helicobacter pylori prevalence and its relationship to patients with diabetes mellitus type-2 (T2DM). Aim: The current study was carried out to detect the sero-frequency of Helicobacter pylori among

Research paper thumbnail of Sero-frequency of Hepatitis B Infection among Health Care Workers in Khartoum

Background: Health care workers (HCWs) are at a great risk of occupational exposure with

Research paper thumbnail of Sensitivity and Specificity of Rapid Antigen Test in Diagnosis of Streptococcal Upper Respiratory Tract Infection

Background: Sore throat is one of the most common reasons for visits to family physicians.

Research paper thumbnail of Panton-Valentine leukocidin gene Association with Methicillin-resistant and sensitive Staphylococcus aureus strains isolated from Community and Hospital-acquired wound infections in Khartoum-Sudan

BACKGROUND: Panton-Valentine leukocidin (PVL) genes encode a potent toxin in

Research paper thumbnail of Panton-Valentine Leukocidin Gene among Hospital Acquired and Community Acquired Methicillin Resistant Staphylococcus aureus Isolates from Patients in Khartoum-Sudan

Background: Panton-Valentine Leukocidin (PVL) genes encode a highly potent toxin involved in seve... more Background: Panton-Valentine Leukocidin (PVL) genes encode a highly potent toxin involved in severe skin infections and necrotizing pneumonia, even in healthy individuals. Infections with PVL producing Staphylococcu aureus strains have increased over the last decade and attracted the attention twards the detection of the PVL toxin.

Research paper thumbnail of PREVALENCE OF HUMAN IMMUNODEFICIENCY VIRUS INFECTION AMONG TUBERCULOSIS PATIENTS IN OMDURMAN -SUDAN

Research paper thumbnail of PREVALENCE OF HERPES SIMPLEX VIRUS 1/2 AND HUMAN CYTOMEGALOVIRUS IN SUDANESE SCHIZOPHRENIC PATIENTS

Background: Cognitive impairment in the form of decreased working memory and executive functions ... more Background: Cognitive impairment in the form of decreased working memory and executive functions has been recognized as a key deficit in schizophrenia. Neurotropic viruses such as Herpes virus and human cytomegalovirus have been associated with focal gray matter deficits in patients with schizophrenia. Aim: The study was carried out to detect the prevalence and relationship of herpes simplex virus (HSV 1/2) and human cytomegalovirus (HCMV) among Sudanese schizophrenia patients. Methods: A total number of 60 plasma samples were collected randomly from schizophrenia patients at Kubar hospital in Khartoum, Sudan. On the other hand, 30 samples were collected from healthy individuals were served as controls. The detection of HSV1/2 and HCMV IgG antibodies were performed by using an Enzyme-linked immunosorbent assay (ELISA). The results: HSV1/2 IgG antibodies were detected in 95% schizophrenic patients and 93.3% control (0.75 P value), while IgG antibodies to HCMV were detected in 96.7% schizophrenic patients and (90.0%) control (P value 0.19).

Research paper thumbnail of Prevalence and Antimicrobial Susceptibility Pattern of Methicillin Resistance Staphylococcus aureus in Khartoum Sudan

Methicillin-resistant Staphylococcus aureus (MRSA) has become increasingly prevalent worldwide. M... more Methicillin-resistant Staphylococcus aureus (MRSA) has become increasingly prevalent worldwide. Multi-resistant MRSA has been commonly reported to be one of the commonest causes of nosocomial infections worldwide. Also, recent reports describe MRSA in community. The aim of this study was to determine the prevalence of MRSA and their antimicrobial susceptibility pattern among hospital and community acquired Staphylococcus aureus isolates from Khartoum hospitals (Sudan), from September 2010 to September 2011. This is a cross sectional study. Staphylococcus aureus strains were isolated and identified from patients suffering from skin and wound infections in Khartoum using conventional microbiology techniques. MRSA strains were investigated by detection of mecA gene using PCR. MRSA strains were also tested for antimicrobial resistance using Kirby-Bauer NCCLS modified disc diffusion technique. A total of 223 strains of Staphylococcus aureus isolates were collected from 400 patients suffering wound and skin infections 46.2% (103/223) strains were from community acquired infections, and 53.8% (120/223) strains were from hospital acquired infections, 78 out of 223 (35.3%) were found to be

Research paper thumbnail of Laboratory medicine journal Prevalence and Antimicrobial Susceptibility Pattern of Extended Spectrum β-Lactamases Producing Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis in Khartoum Sudan

Background: Resistance to broad-spectrum β-lactams, mediated by extended-spectrum β lactamase enz... more Background: Resistance to broad-spectrum β-lactams, mediated by extended-spectrum β lactamase enzymes (ESBL), has become increasingly prevalent worldwide. Objective: The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of extended spectrum β-lactamases producing Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis isolated from clinical specimens. Methods: This is across sectional study. A total of

Research paper thumbnail of PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF BACTERIA CAUSING POSTOPERATIVE WOUND INFECTIONS IN PORT-SUDAN

Research paper thumbnail of FREQUENCY OF HEPATITIS E VIRUS AMONG RENAL TRANSPLANT RECIPIENTS PATIENTS IN KHARTOUM -SUDAN

Research paper thumbnail of Frequency of Hepatitis E Virus among Pregnant Women Attending Khartoum Hospitals

Background: Hepatitis E virus (HEV) causes large-scale epidemics of acute viral hepatitis, partic... more Background: Hepatitis E virus (HEV) causes large-scale epidemics of acute viral hepatitis, particularly in developing countries with high mortality rate among pregnant women. The infection is more severe, often leading to fulminant hepatic failure and death in a significant proportion of patients. Objective: The aim of this study was to verify the frequency of Hepatitis E virus among pregnant women attending Khartoum hospitals. Methods: Enzyme linked immunoassay (ELISA) was done to determine the presence of anti-HEV IgG among 90 pregnant women during the period from July to September 2013. The results: HEV IgG antibodies were detected in 41.1% (37/90). The highest percentages were recorded in the second and third trimesters of pregnancies (37.8% and 48.7%) respectively. Conclusion: This study found a high frequency of anti-HEV IgG among Sudanese pregnant women in Khartoum.

Research paper thumbnail of Laboratory medicine journal Detection of Carbapenem-Resistant Escherichia coli, Klebsiellapneumoniae and Proteus mirabilis clinical isolates in Khartoum; Sudan

Background: Carbapenem resistantGram-negativerods are a group of emerging, highly drug

Research paper thumbnail of COMPARISON OF TYPHIDOT-EIA AND WIDAL TEST IN RESPECT TO POLYMERASE CHAIN REACTION AS DIAGNOSTIC PROCEDURES FOR EARLY DIAGNOSIS OF TYPHOID FEVER

Research paper thumbnail of Antimicrobial Resistance of Escherichia coli Strains isolated from Community and Hospital-acquired Urinary Tract Infections in Khartoum (Sudan

Background: Urinary tract infections (UTIs) are among the most common indications for

Research paper thumbnail of VANCOMYCIN RESISTANCE AMONG METHICILLIN RESISTANT Staphylococcus aureus ISOLATES IN KHARTOUM-SUDAN

Background: Glycopeptides such as vancomycin are frequently the antibiotics of choice for the tre... more Background: Glycopeptides such as vancomycin are frequently the antibiotics of choice for the treatment of infections caused by methicillin resistant Staphylococcus aureus. For the last 10 years incidence of vancomycin intermediate S. aureus and vancomycin resistant S. aureus has been increasing in various parts of the world Objective: The aim of this study was to determine the prevalence of vancomycin intermediate S. aureus and vancomycin resistant S. aureus and their antimicrobial susceptibility pattern among hospital and community acquired Methicillin Resistant Staphylococcus aureus isolates.