Harjeet Singh | National Institute Of Technology Kurukshetra (original) (raw)

Harjeet Singh

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Papers by Harjeet Singh

Research paper thumbnail of Manufacture of T cells using the Sleeping Beauty system to enforce expression of a CD19-specific chimeric antigen receptor

Cancer gene therapy, 2015

T cells can be reprogrammed to redirect specificity to tumor-associated antigens (TAAs) through t... more T cells can be reprogrammed to redirect specificity to tumor-associated antigens (TAAs) through the enforced expression of chimeric antigen receptors (CARs). The prototypical CAR is a single-chain molecule that docks with TAA expressed on the cell surface and, in contrast to the T-cell receptor complex, recognizes target cells independent of human leukocyte antigen. The bioprocessing to generate CAR(+) T cells has been reduced to clinical practice based on two common steps that are accomplished in compliance with current good manufacturing practice. These are (1) gene transfer to stably integrate the CAR using viral and nonviral approaches and (2) activating the T cells for proliferation by crosslinking CD3 or antigen-driven numeric expansion using activating and propagating cells (AaPCs). Here, we outline our approach to nonviral gene transfer using the Sleeping Beauty system and the selective propagation of CD19-specific CAR(+) T cells on AaPCs.

Research paper thumbnail of Identifying candidate allogeneic NK-cell donors for hematopoietic stem-cell transplantation based on functional phenotype

Research paper thumbnail of PET imaging of T cells derived from umbilical cord blood

Research paper thumbnail of Prosthetic Valve Leakage following Cardiac Valve Replacements

CHEST Journal, 1972

Thirty cases were evaluated in order to study the etiology of prosthetic valvular dehiscence in v... more Thirty cases were evaluated in order to study the etiology of prosthetic valvular dehiscence in view of its continued occurrence in a small percentage of cases. The clinical course, management, initial valvular pathology, as well as findings at reoperation and necropsy were the main basis of this study. Factors in the genesis of prosthetic valvular dehiscence in the early and late groups seemed to be distinctly different. Of the 24 patients in the early group, defined as those with evident regurgitation within six months of the operation, in 12 the stitches, which were well tied in, had come loose from the tissue bed. The left ventricular contraction was believed to be the disrupting force in at least six patients with grossly dilated mitral ring.

Research paper thumbnail of Analysis of Layered Protocol Structure of TCP/IP Communication Networks Using Wireshark Protocol Analyser

TCP/IP is a family of communication protocols used to connect computer systems in a network. It i... more TCP/IP is a family of communication protocols used to connect computer systems in a network. It is named after two of the protocols in the family: Transmission Control Protocol (TCP) and Internet Protocol (IP). Hypertext Transfer Protocol (HTTP) is a member of the TCP/IP family.
The protocols in the TCP/IP family correspond, in many cases, to the layers of the Open Systems Interconnection (OSI) model. Table 1 shows HTTP and the underlying layers of the TCP/IP family in terms of the OSI model. The Systems Network Architecture (SNA) layers, which match the OSI layers, are also shown.

Research paper thumbnail of DISEASE PREDICTION SYSTEM USING SYMPTOMS

IRJET, 2023

Technology has revolutionized the health domain largely. This project aims to design a diagnostic... more Technology has revolutionized the health domain largely. This project aims to design a diagnostic model for various diseases relying on their symptoms. This System has used data mining techniques such as classification in order to achieve such a model. Datasets consisting of voluminous data about patient diseases are gathered, refined, classified, and used for training the intelligent agent. Here, the Naive Bayes Algorithm is used for classification purposes. Naïve Bayes Classifier calculates the probabilities of the disease. Based on the result, the patient can contact the doctor accordingly for further treatment. It is an exemplar where technology and health knowledge are sewn into a thread perfectly with a desire to achieve "prediction is better than cure"

Research paper thumbnail of Manufacture of T cells using the Sleeping Beauty system to enforce expression of a CD19-specific chimeric antigen receptor

Cancer gene therapy, 2015

T cells can be reprogrammed to redirect specificity to tumor-associated antigens (TAAs) through t... more T cells can be reprogrammed to redirect specificity to tumor-associated antigens (TAAs) through the enforced expression of chimeric antigen receptors (CARs). The prototypical CAR is a single-chain molecule that docks with TAA expressed on the cell surface and, in contrast to the T-cell receptor complex, recognizes target cells independent of human leukocyte antigen. The bioprocessing to generate CAR(+) T cells has been reduced to clinical practice based on two common steps that are accomplished in compliance with current good manufacturing practice. These are (1) gene transfer to stably integrate the CAR using viral and nonviral approaches and (2) activating the T cells for proliferation by crosslinking CD3 or antigen-driven numeric expansion using activating and propagating cells (AaPCs). Here, we outline our approach to nonviral gene transfer using the Sleeping Beauty system and the selective propagation of CD19-specific CAR(+) T cells on AaPCs.

Research paper thumbnail of Identifying candidate allogeneic NK-cell donors for hematopoietic stem-cell transplantation based on functional phenotype

Research paper thumbnail of PET imaging of T cells derived from umbilical cord blood

Research paper thumbnail of Prosthetic Valve Leakage following Cardiac Valve Replacements

CHEST Journal, 1972

Thirty cases were evaluated in order to study the etiology of prosthetic valvular dehiscence in v... more Thirty cases were evaluated in order to study the etiology of prosthetic valvular dehiscence in view of its continued occurrence in a small percentage of cases. The clinical course, management, initial valvular pathology, as well as findings at reoperation and necropsy were the main basis of this study. Factors in the genesis of prosthetic valvular dehiscence in the early and late groups seemed to be distinctly different. Of the 24 patients in the early group, defined as those with evident regurgitation within six months of the operation, in 12 the stitches, which were well tied in, had come loose from the tissue bed. The left ventricular contraction was believed to be the disrupting force in at least six patients with grossly dilated mitral ring.

Research paper thumbnail of Analysis of Layered Protocol Structure of TCP/IP Communication Networks Using Wireshark Protocol Analyser

TCP/IP is a family of communication protocols used to connect computer systems in a network. It i... more TCP/IP is a family of communication protocols used to connect computer systems in a network. It is named after two of the protocols in the family: Transmission Control Protocol (TCP) and Internet Protocol (IP). Hypertext Transfer Protocol (HTTP) is a member of the TCP/IP family.
The protocols in the TCP/IP family correspond, in many cases, to the layers of the Open Systems Interconnection (OSI) model. Table 1 shows HTTP and the underlying layers of the TCP/IP family in terms of the OSI model. The Systems Network Architecture (SNA) layers, which match the OSI layers, are also shown.

Research paper thumbnail of DISEASE PREDICTION SYSTEM USING SYMPTOMS

IRJET, 2023

Technology has revolutionized the health domain largely. This project aims to design a diagnostic... more Technology has revolutionized the health domain largely. This project aims to design a diagnostic model for various diseases relying on their symptoms. This System has used data mining techniques such as classification in order to achieve such a model. Datasets consisting of voluminous data about patient diseases are gathered, refined, classified, and used for training the intelligent agent. Here, the Naive Bayes Algorithm is used for classification purposes. Naïve Bayes Classifier calculates the probabilities of the disease. Based on the result, the patient can contact the doctor accordingly for further treatment. It is an exemplar where technology and health knowledge are sewn into a thread perfectly with a desire to achieve "prediction is better than cure"

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