Vivek Javalkote | North Maharashtra University (original) (raw)

Patent by Vivek Javalkote

A safe, easy-to-use, cost effective liquid medium capable of dissolution of maximum amount of flu... more A safe, easy-to-use, cost effective liquid medium capable of dissolution of maximum amount of flue gases is described. The components of the liquid medium are also preferably industrial waste chemicals. The medium is used for developing a process for trapping of flue gases generated due to combustion of fuel in generators. The maximum trapping capacity of the medium is achieved and the saturated medium is used for growing algal culture by releasing into open raceway ponds. Optionally, other substances, enhancing algal growth may be added to the saturated medium. Harvested culture is dried in natural sunlight and final biomass in powder form is produced.

Feed supplement is a good source of providing protein, essential fatty acids and pigments for goo... more Feed supplement is a good source of providing protein, essential fatty acids and pigments for good growth and health of poultry. The main problem of feed supplement is that many of the nutritional components in the feed are easily degraded upon drying, improper storage and adverse conditions such as exposure to heat and moisture. The present subject matter provides a crisp, palatable feed supplement and a method of preserving the nutritional components of a feed supplement by encapsulating the feed supplement in a natural, edible encapsulating material.

Papers by Vivek Javalkote

Bioresource Technology, 2016

The present study reports a process wherein CO 2 is captured in the form of bicarbonates using ca... more The present study reports a process wherein CO 2 is captured in the form of bicarbonates using calcium oxide and photosynthetically fixed into biomass. Microalgal cultures viz. Chlorella sorokiniana PAZ and Arthrospira sp. VSJ were grown in the medium containing bicarbonates. The rate of bicarbonate utilization by C. sorokiniana PAZ was higher when CO 2 trapped in the presence of 2.67 mM calcium oxide than in the presence of 10 mM sodium hydroxide and with direct addition of 10 mM sodium bicarbonate. For Arthrospira sp. VSJ the bicarbonate utilization was 92.37 %, 88.34 % and 59.23 % for the medium containing CaO, NaOH and NaHCO 3 , respectively. Illumination of photosynthetically active radiation (PAR) + ultraviolet A radiation (UVA) enhanced the yield of C. sorokiniana PAZ and Arthrospira sp. VSJ by 1.3 and 1.8 folds, respectively. FTIR analysis revealed elevation in the biosynthesis of specific metabolites in response to the UVA exposure.

Applied and Environmental Microbiology, 2016

The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological ... more The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms. MAAs have a range of physiological functions, including protection against UV radiation, making them important from a biotechnological perspective. In the present study, we identified a putative MAA ( mys ) gene cluster in two New Zealand isolates of Scytonema cf. crispum (UCFS10 and UCFS15). Homology to “ Anabaena -type” mys clusters suggested that this cluster was likely to be involved in shinorine biosynthesis. Surprisingly, high-performance liquid chromatography analysis of S . cf. crispum cell extracts revealed a complex MAA profile, including shinorine, palythine-serine, and their hexose-bound variants. It was hypothesized that a short-chain dehydrogenase (UCFS15_00405) encoded by a gene adjacent to the S . cf. crispum mys cluster was responsible for the conversion of shinorine to palythine-serine. Heterologous expression of MysABCE and UCFS15_004...

Applied microbiology and biotechnology, Jan 8, 2015

The present study is an investigation into the adsorption of zinc metal on marine diatom Nitzschi... more The present study is an investigation into the adsorption of zinc metal on marine diatom Nitzschia sp. under the influence of ultraviolet radiation to reveal the possible role of radiation in metal biomineralization. Nitzschia sp. was isolated from marine habitat and gradually acclimatized to UVA, UVB, and zinc (30 mg/L). The culture was subjected to various concentrations of zinc (5, 10, 15, 20 mg/L) with an artificial exposure of PAR, PAR + UVA, and PAR + UVB radiation. The adsorption isotherm models were applied for validating the hypothesis stating the association of zinc adsorption with exposure to ultraviolet radiation. Altered functional groups on the surface were observed in the FTIR spectra along with changes in antioxidant activity, superoxide dismutase activity, pigment and protein contents due to increased zinc concentration and ultraviolet radiation. Scanning electron micrographs (SEM-EDAX) supported the microscopic morphology of adsorbed metals.

Materials Science and Engineering: C, 2015

In the present investigation, curcumin loaded magnetically active frustules have been reported. T... more In the present investigation, curcumin loaded magnetically active frustules have been reported. The diatoms were cultured and frustules were obtained by chemical and thermal process. The frustules were rendered magnetically active by incorporation of iron oxide nanoparticle using two different method involving ferrofluid (CMDM-F) and in situ synthesis (CMDM-I) of iron oxide nanoparticle. These CMDM prepared by two techniques were characterized using FT-IR and vibrating sample magnetometer (VSM) analysis. Particle size and potential were measured using the Malvern Zetasizer. Scanning electron microscopy (SEM) was utilized for studying the surface morphology of CMDM, and in addition to this elemental analysis was also performed for confirming the presence of iron. The cell viability assay was carried out using the HeLa cell line. SEM images showed a change in surface morphology of diatoms before and after rendering magnetic activity. Cell viability assay revealed that CMDM-F had reasonably high cytotoxicity (60.2%) compared to Curcumin (42.1%), DM (1.9%), CDM (44.8%), CMDM-I (59.9). Both, CMDM-F and CMDM-I showed improved cytotoxicity when compared with pure curcumin. The overall study suggests that the developed CMDM could be utilized as a potential carrier to deliver cargo for efficient chemotherapy.

Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion from... more Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion from today. To meet the demands of growing, geographically and socio-economically diversified nations, we need to diversity and expand agricultural production. This expansion of agricultural productivity will need to occur under increasing biotic, and environmental constraints driven by climate change. Clustered regularly interspaced short palindromic repeats-site directed nucleases (CRISPR-SDN) and similar genome editing technologies will likely be key enablers to meet future agricultural needs. While the application of CRISPR-Cas9 mediated genome editing has led the way, the use of CRISPR-Cas12a is also increasing significantly for genome engineering of plants. The popularity of the CRISPR-Cas12a, the type V (class-II) system, is gaining momentum because of its versatility and simplified features. These include the use of a small guide RNA devoid of trans-activating crispr RNA (tracrRNA), ...

COVID-19 represents an unprecedented threat to global public health and economies. Assays are urg... more COVID-19 represents an unprecedented threat to global public health and economies. Assays are urgently needed for rapid detection and diagnosis of SARS-CoV-2-infected patients in order to inform treatment and quarantine strategies. Establishing globally accepted easy-to-access diagnostic tests is extremely important to understanding the epidemiology of the present pandemic. While nucleic acid-based tests are considered to be more sensitive with respect to serological tests, but the present gold standard RT-PCR-based assays possess limitations such as low sample throughput, requirement for sophisticated reagents and instrumentation. To overcome these shortcomings, recent efforts of incorporating LAMP-based isothermal detection, and minimizing the number of reagents required are on rise. Novel CRISPR- and other nuclease-based techniques, when merge with isothermal and allied technologies, promises to provide sensitive and rapid detection of SARS-CoV-2 nucleic acids. Here we discuss an...

The present study reports a process wherein CO2 is captured in the form of bicarbonates using cal... more The present study reports a process wherein CO2 is captured in the form of bicarbonates using calcium oxide and photosynthetically fixed into biomass. Microalgal cultures viz. Chlorella sorokiniana PAZ and Arthrospira sp. VSJ were grown in the medium containing bicarbonates. The rate of bicarbonate utilization by C. sorokiniana PAZ was higher when CO2 trapped in the presence of 2.67 mM calcium oxide than in the presence of 10 mM sodium hydroxide and with direct addition of 10 mM sodium bicarbonate. For Arthrospira sp. VSJ the bicarbonate utilization was 92.37%, 88.34% and 59.23% for the medium containing CaO, NaOH and NaHCO3, respectively. Illumination of photosynthetically active radiation (PAR) + ultraviolet A radiation (UVA) enhanced the yield of C. sorokiniana PAZ and Arthrospira sp. VSJ by 1.3 and 1.8 folds, respectively. FTIR analysis revealed elevation in the biosynthesis of specific metabolites in response to the UVA exposure.

The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological ... more The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms.
MAAs have a range of physiological functions, including protection against UV radiation, making them important
from a biotechnological perspective. In the present study, we identified a putative MAA (mys) gene cluster in two New Zealand
isolates of Scytonema cf. crispum (UCFS10 and UCFS15). Homology to “Anabaena-type” mys clusters suggested that this cluster
was likely to be involved in shinorine biosynthesis. Surprisingly, high-performance liquid chromatography analysis of S. cf.
crispum cell extracts revealed a complex MAA profile, including shinorine, palythine-serine, and their hexose-bound variants. It
was hypothesized that a short-chain dehydrogenase (UCFS15_00405) encoded by a gene adjacent to the S. cf. crispum mys cluster
was responsible for the conversion of shinorine to palythine-serine. Heterologous expression of MysABCE and UCFS15_00405
in Escherichia coli resulted in the exclusive production of the parent compound shinorine. Taken together, these results suggest
that shinorine biosynthesis in S. cf. crispum proceeds via an Anabaena-type mechanism and that the genes responsible for the
production of other MAA analogues, including palythine-serine and glycosylated analogues, may be located elsewhere in the genome.

Culture conditions for maximum dry weight production were optimized using response surface method... more Culture conditions for maximum dry weight production were optimized using response surface methodology. Central Composite Design (CCD) was used to access the effect of three parameters i.e. HCO3 ˉ concentration, pH of culture medium and cobalt ion concentration on maximum dry weight production of green microalgae Chlorella sorokiniana PAZ. The experiments estimated the optimized conditions as, 1127 mg/l for HCO3 ˉ , 7.86 for pH and 13.07 mg/l for Co 2+. Highest predicted biomass for Chlorella sorokiniana PAZ was 0.891g/l using response surface methodology.

In the present investigation, curcumin loaded magnetically active frustules have been reported. ... more In the present investigation, curcumin loaded magnetically active frustules have been
reported. The diatoms were cultured and frustules were obtained by chemical and thermal
process. The frustules were rendered magnetically active by incorporation of iron oxide
nanoparticle using two different method involving ferrofluid (CMDM-F) and in situ synthesis
(CMDM-I) of iron oxide nanoparticle. These CMDM prepared by two techniques were
characterized using FT-IR and vibrating sample magnetometer (VSM) analysis. Particle size
and potential were measured using the Malvern Zetasizer. Scanning electron microscopy
(SEM) was utilized for studying the surface morphology of CMDM, and in addition to this
elemental analysis was also performed for confirming the presence of iron. The cell viability
assay was carried out using the HeLa cell line. SEM images showed a change in surface
morphology of diatoms before and after rendering magnetic activity. Cell viability assay
revealed that CMDM-F had reasonably high cytotoxicity (60.2%) compared to Curcumin
(42.1%), DM (1.9%), CDM (44.8%), CMDM-I (59.9). Both, CMDM-F and CMDM-I
showed improved cytotoxicity when compared with pure curcumin. The overall study
suggests that the developed CMDM could be utilized as a potential carrier to deliver cargo
for efficient chemotherapy.

Bioresource Technology, 2016

Applied and Environmental Microbiology, 2016

Applied microbiology and biotechnology, Jan 8, 2015

Materials Science and Engineering: C, 2015

The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological ... more The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms.
MAAs have a range of physiological functions, including protection against UV radiation, making them important
from a biotechnological perspective. In the present study, we identified a putative MAA (mys) gene cluster in two New Zealand
isolates of Scytonema cf. crispum (UCFS10 and UCFS15). Homology to “Anabaena-type” mys clusters suggested that this cluster
was likely to be involved in shinorine biosynthesis. Surprisingly, high-performance liquid chromatography analysis of S. cf.
crispum cell extracts revealed a complex MAA profile, including shinorine, palythine-serine, and their hexose-bound variants. It
was hypothesized that a short-chain dehydrogenase (UCFS15_00405) encoded by a gene adjacent to the S. cf. crispum mys cluster
was responsible for the conversion of shinorine to palythine-serine. Heterologous expression of MysABCE and UCFS15_00405
in Escherichia coli resulted in the exclusive production of the parent compound shinorine. Taken together, these results suggest
that shinorine biosynthesis in S. cf. crispum proceeds via an Anabaena-type mechanism and that the genes responsible for the
production of other MAA analogues, including palythine-serine and glycosylated analogues, may be located elsewhere in the genome.