Mark Cannon | Northwestern University (original) (raw)
Papers by Mark Cannon
This pilot study of Streptococcus mutans ATCC 35668 grown in media with and without polyols (eryt... more This pilot study of Streptococcus mutans ATCC 35668 grown in media with and without polyols (erythritol) measured the resultant metabolites, including the short chain fatty acids by using head space analysis. Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose containing no polyols or either erythritol or xylitol and Streptococcus mutans (ATCC 35668) was grown aerobically. After 48 hours of growth the supernatant were harvested and centrifuged to pellet bacteria. Supernatants were removed from bacterial pellets then submitted for Short Chain Fatty Acid (SCFA) analysis with an Agilent Technologies (Santa Clara, CA 95051) system configured from three components, a 5973 mass selective detector, a 6890N gas chromatographer, and a 7697A headspace sampler. Streptococcus mutans growing in Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose but containing no polyols produced the following short chain fatty acids: methyl isovalerate, acet...
Journal of Clinical Pediatric Dentistry, 2014
Aim: The purpose of this in vivo study was to compare the effectiveness of a new light cured resi... more Aim: The purpose of this in vivo study was to compare the effectiveness of a new light cured resin based dicalcium/tricalcium silicate pulp capping material (TheraCal LC, Bisco), pure Portland cement, resin based calcium hydroxide or glass ionomer in the healing of bacterially contaminated primate pulps. Study design: The experiment required four primates each having 12 teeth prepared with buccal penetrations into the pulpal tissues with an exposure of approximately 1.0 mm. The exposed pulps of the primate teeth were covered with cotton pellets soaked in a bacterial mixture consisting of microorganisms normally found in human pulpal abscesses. After removal of the pellet, hemostasis was obtained and the pulp capping agents applied. The light cured resin based pulp capping material (TheraCal LC) was applied to the pulpal tissue of twelve teeth with a needle tip syringe and light cured for 15 seconds. Pure Portland cement mixed with a 2% Chlorhexidine solution was placed on the expose...
Interventions in Pediatric Dentistry Open Access Journal, 2019
The oral systemic health link has now become well recognized and numerous publications have discu... more The oral systemic health link has now become well recognized and numerous publications have discussed the importance of the oral microbiome in the overall health of any individual. This is likely even more important in children and this influences their future health as they mature. What is particularly interesting is that the microbiome of a child is developed prior to birth and is related to the maternal oral microbiome. Early intervention to the mother prior to the child's birth, with proper sleep, exercise and dietary adjustments such as the limitation of added sugars and use of coconut oil, for example, will influence the developing child's microbiome. Direct influence with the polyols xylitol and erythritol to reduce pathogens, and probiotics to boost levels of commensals would also be required. Due to the extent of oral disease, and its tremendous associated costs, urgent action is required by all health professions.
Viral pandemics have taken a significant toll on humanity and the world now is contending with th... more Viral pandemics have taken a significant toll on humanity and the world now is contending with the SARS-CoV-2 epidemic. Readily available economical preventive measures should be immediately explored. Xylitol has been reported to reduce the severity of viral infections as well as the severity of pneumonia, and increase the survivability of animal subjects. Since pneumonia and acute respiratory distress syndrome are potentially fatal complications of COVID-19, the present study tested the in vitro effectiveness of xylitol against SARS-CoV-2. Virus titers and LRV of SARS-CoV-2, were incubated with a single concentration of nasal spray. Toxicity was observed in the top dilution (1/10). Virus was seen below that dilution so it did not affect calculations of virus titer or LRV. After a 25-minute contact time, the nasal spray (11% Pure Xylitol, 0.85%NaCL (Saline), and 0.20% grapefruit seed extract) reduced virus from 4.2 to 1.7 log10 CCID50 per 0.1 mL, a statistically significant reductio...
Journal of applied oral science : revista FOB
Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized... more Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHA...
Probiotics and Antimicrobial Proteins, May 7, 2011
Probiotics have been widely publicized in the general press and the consumer media. Knowledge of ... more Probiotics have been widely publicized in the general press and the consumer media. Knowledge of the existence of "probiotics" is commonplace, and the effectiveness of probiotic therapy has been well reported in the medical literature. However, even though most published dental studies have reported positive results, the dental profession has not yet accepted the use of probiotic therapy as an adjunct for preventive dental care. This review article discusses published and current research into the applications of probiotics along with diagnostic testing of the oral biofilm. Probiotic therapy appears to be generally safe and effective in modifying with beneficial bacteria the oral biofilm and thereby reducing the effects of pathogenic oral bacteria. In this review, some examples of current oral probiotic research are discussed along with reference to the potential application of diagnostic testing of the oral biofilm for the presence of oral pathogens as a precursor to initiation of specific probiotic therapy. Dental professionals should be actively investigating this potentially very useful therapeutic measure for the benefit of their patients.
Journal of Dentistry For Children, 2005
The objective of this study was to evaluate the position of the mandibular lingula (ML) to provid... more The objective of this study was to evaluate the position of the mandibular lingula (ML) to provide data for inferior alveolar nerve block techniques in children. One hundred fifty-four panoramic radiographs of 7- to 10-year-old boys and girls were analyzed. Measurements were taken from the ML to the occlusal plane, and the percentile distances of the ML to ramal borders were determined. The distance between the ML and the occlusal plane showed a gradual increase, but only in the male group was it statistically significant. ML's ratio position on the ramus remained constant in all analyzed groups. In the 7-year-old group, the ML was observed above the occlusal plane in 70% of girls and 55% of boys. That percentage reached 85% of all children by age 10. The mandibular lingula's ratio position remained constant. Inferior alveolar anesthesia should be administered at least 6 mm above the occlusal plane in 7- to 8-year-old children, while 10 mm could be indicated for 9- to 10-year-old children. The mandibular lingula should be considered a reliable reference for further…
Journal of Dentistry For Children, 2003
This study evaluated the clinical efficacy of the &am... more This study evaluated the clinical efficacy of the "open sandwich" restoration for pediatric dental practice. Three pediatric dentists used a standardized preparation and restorative technique to place the restorations. The prepared tooth was etched with a phosphoric acid semigel and rinsed. A resin modified glass ionomer (Fuji II LCor Photac-Fil) was placed short of the margins and then light cured. The resin modified glass ionomer was covered with an occlusal layer of a microhybrid flowable composite (Aeliteflo or Flow-it). The same preparation for the experimental restorations was used for the control conventional amalgam (Tytin) restorations. The restorations were evaluated at 6-month intervals and ranked with a modified United States Public Heath Survey (USPHS) scale as follows: Alfa: No discernible marginal opening or stain; Beta: Slight opening of margin discernible with dental explorer, but without stain; Charlie: Open margin and stain; Delta: Recurrent caries or restoration failure. Restoration failures were categorized according to etiology, pulpal necrosis, bruxism, marginal leakage, isthmus fracture, or adhesive failure. All recalled experimental restorations, except 8, were rated as either Alpha or Beta. Six failed due to isthmus fracture and 2 due to pulpal necrosis. Fifteen restorations had delaminating of the flowable composite from the resin modified glass ionomer. The use of the "open sandwich" technique compared favorably with a similar study using adhesive amalgam restorations. The "open sandwich" technique can be successfully used in a pediatric dental practice.
American Journal of Dentistry, Aug 1, 2013
To evaluate the biochemical and microbiological characteristics of in situ biofilm formed on mate... more To evaluate the biochemical and microbiological characteristics of in situ biofilm formed on materials that release fluoride (F-) or calcium (Ca++) and phosphate (Pi). This study comprised an in situ and in vitro experiment, utilizing three materials [Auralay XF and Fuji IX GP, containing fluoride, and Aegis containing amorphous calcium phosphate (ACP)] and bovine dental enamel slabs. For the in situ: 10 volunteers wore palatal devices, each containing four material specimens or enamel slabs that were treated with 20% sucrose solution. The biofilm had pH measurements on Day 7 and the composition was analyzed on Day 8 by assessing the following: F-, Ca++, Pi and insoluble extracellular polysaccharides (EPS) concentrations, and then identification of the microbiota. For the in vitro: materials/enamel were subjected to a 7-day pH-cycling regimen to determine F, Ca++ and Pi release. The biofilm formed on F(-)-releasing materials was richer in F, Ca++ and Pi and had lower mutans streptococci counts than enamel biofilm. The biofilm on the ACP-containing material exhibited similar Ca++ and Pi concentrations to biofilm on F(-)-releasing materials. The materials showed buffering action compared with enamel. Biochemical and microbiological characteristics showed a less cariogenic biofilm on materials containing fluoride or amorphous calcium phosphate.
Pediatric dentistry, 1983
... Ira N. Moyer, DDS Roger H. Kobayashi, MD Mark L. Cannon, DDS, MS John F. Simon, DDS, MS Rober... more ... Ira N. Moyer, DDS Roger H. Kobayashi, MD Mark L. Cannon, DDS, MS John F. Simon, DDS, MS Robert O. Cooley, DDS, MS ... In one variant of this disease, a missing enzyme, adenine deaminase (ADA), leads to the accumulation of metabolic by-products which are toxic to the ...
Compendium of continuing education in dentistry (Jamesburg, N.J. : 1995), 2003
The introduction of low-shrinkage composite and no-rinse conditioners has created an opportunity ... more The introduction of low-shrinkage composite and no-rinse conditioners has created an opportunity for pediatric dentists to change their standard operative regimen. The low-shrink composite solves many of the problems that have discouraged clinicians from routinely providing posterior composites for their patients. The reduction in polymerization shrinkage decreases problems with contraction stresses, sensitivity, microleakage, recurrent caries, and negative pulpal sequelae. No-rinse conditioners simplify the process and shorten the time required for bonding procedures. Liquid polish reduces the tedious finishing previously required with resin-based composites. The combination of the easier bonding and more user-friendly composite could add a new operative technique to the pediatric dentist's armamentarium. A study of low-shrink, resin-based composite restorations was performed to determine the effectiveness of these new materials for pediatric dental practice. The restorations w...
Compendium of continuing education in dentistry (Jamesburg, N.J. : 1995), 2013
The Journal of clinical pediatric dentistry, 2013
This IRB approved clinical trial was to determine the effect of "over the counter" prob... more This IRB approved clinical trial was to determine the effect of "over the counter" probiotic supplements on the Caries Risk Test- CRT- (Ivoclar) results of the oral microflora in high caries risk children. Sixty subjects 6 to 12 years old with a caries risk assessment (CAMBRA) of moderate to high (caries prone) were evaluated by an analysis of the difference in the salivary levels of pathogenic bacteria (mutans streptococci and Lactobacilli). The subjects were randomly selected by randomizing software and assigned to two diferent Groups. Group A used PerioBalance (Lactobacilli reuteri-CFU of 200 million) lozenges for 28 days. Group B used the EvoraKids (Streptococcus uberis KJ2, Streptococcus oralis KJ3, Streptococcus rattus JH1 45, > or = 100 million) probiotics chewable tablets for 30 days. Salivary samples were collected then incubated for 48 hours for colony counting and ranking. Follow up testing with the CRT was performed after 60 days at a follow up visit. There ...
Encyclopedia of Medical Devices and Instrumentation, 2006
... 17. Sterman S, Marsden J. Silane coupling agents as integral blends in resin-filler systems. ... more ... 17. Sterman S, Marsden J. Silane coupling agents as integral blends in resin-filler systems. Mod Plastics 1963;49(11):125. ... Deliperi S, Bardwell D. An alternative method to reduce polymerization shrinkage in direct posterior composite restorations. JADA 2002;133:1387–1398. ...
Journal of Endodontics, 2008
The purpose of this study was to evaluate the subcutaneous response of rat connective tissue to l... more The purpose of this study was to evaluate the subcutaneous response of rat connective tissue to light-cure MTA and Ângelus MTA. These materials were placed in polyethylene and dentin tubes and implanted into dorsal connective tissue of Wistar rats for 30 and 60 days. The specimens were prepared to be stained with hematoxylin-eosin, Von Kossa, and without stain for polarized light and evaluated in an optic microscope. The Ângelus MTA showed a mild inflammatory response at 30 days and none at 60 days, characterized by organized connective tissue, presence of some chronic inflammatory cells, and induction of mineralized tissue formation. Light-cure MTA presented a moderate chronic inflammatory response at 30 days that decreased at 60 days but was more intense than with Ângelus MTA and without dystrophic calcifications. It was possible to conclude that light-cure MTA was similar to MTA at 60 days, but it did not stimulate mineralization.
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2010
The aim of this study was to evaluate the rat alveolar socket response to implanted polyethylene ... more The aim of this study was to evaluate the rat alveolar socket response to implanted polyethylene tubes filled with a light-cured MTA or Angelus MTA. Study design. Fifty-seven Wistar rats received an implant filled with one of the materials in the dental socket of the right central incisor. After 30, 60, and 90 days, animals were killed, and the right hemimaxillas were removed and processed to be analyzed on light microscopy. After embedding in paraffin, 6-m-thick sections were obtained and stained with hematoxylin and eosin. Qualitative and quantitative analyses of the reaction were performed. Results. It was observed that the light-cured MTA presented a similar response compared with Angelus MTA, characterized by the reduction of the chronic inflammatory response and organization of the alveolar bone close to the materials, especially at the 90th day of observation. Conclusions. It was concluded that both materials were well accepted by the alveolar tissue of rats, with the formation of mineralized tissue close to the materials. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;110:e93-e97) MATERIAL AND METHODS Fifty-seven 4-to 6-month-old male Wistar Albino rats weighing 250-280 g were used. The animals were housed in temperature-controlled rooms and received
Operative Dentistry, 2007
Clinical Relevance When considering the individual needs of patients in the clinical setting, it ... more Clinical Relevance When considering the individual needs of patients in the clinical setting, it is important to know the behavior of restorative materials in the oral environment under pH variations.
This pilot study of Streptococcus mutans ATCC 35668 grown in media with and without polyols (eryt... more This pilot study of Streptococcus mutans ATCC 35668 grown in media with and without polyols (erythritol) measured the resultant metabolites, including the short chain fatty acids by using head space analysis. Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose containing no polyols or either erythritol or xylitol and Streptococcus mutans (ATCC 35668) was grown aerobically. After 48 hours of growth the supernatant were harvested and centrifuged to pellet bacteria. Supernatants were removed from bacterial pellets then submitted for Short Chain Fatty Acid (SCFA) analysis with an Agilent Technologies (Santa Clara, CA 95051) system configured from three components, a 5973 mass selective detector, a 6890N gas chromatographer, and a 7697A headspace sampler. Streptococcus mutans growing in Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose but containing no polyols produced the following short chain fatty acids: methyl isovalerate, acet...
Journal of Clinical Pediatric Dentistry, 2014
Aim: The purpose of this in vivo study was to compare the effectiveness of a new light cured resi... more Aim: The purpose of this in vivo study was to compare the effectiveness of a new light cured resin based dicalcium/tricalcium silicate pulp capping material (TheraCal LC, Bisco), pure Portland cement, resin based calcium hydroxide or glass ionomer in the healing of bacterially contaminated primate pulps. Study design: The experiment required four primates each having 12 teeth prepared with buccal penetrations into the pulpal tissues with an exposure of approximately 1.0 mm. The exposed pulps of the primate teeth were covered with cotton pellets soaked in a bacterial mixture consisting of microorganisms normally found in human pulpal abscesses. After removal of the pellet, hemostasis was obtained and the pulp capping agents applied. The light cured resin based pulp capping material (TheraCal LC) was applied to the pulpal tissue of twelve teeth with a needle tip syringe and light cured for 15 seconds. Pure Portland cement mixed with a 2% Chlorhexidine solution was placed on the expose...
Interventions in Pediatric Dentistry Open Access Journal, 2019
The oral systemic health link has now become well recognized and numerous publications have discu... more The oral systemic health link has now become well recognized and numerous publications have discussed the importance of the oral microbiome in the overall health of any individual. This is likely even more important in children and this influences their future health as they mature. What is particularly interesting is that the microbiome of a child is developed prior to birth and is related to the maternal oral microbiome. Early intervention to the mother prior to the child's birth, with proper sleep, exercise and dietary adjustments such as the limitation of added sugars and use of coconut oil, for example, will influence the developing child's microbiome. Direct influence with the polyols xylitol and erythritol to reduce pathogens, and probiotics to boost levels of commensals would also be required. Due to the extent of oral disease, and its tremendous associated costs, urgent action is required by all health professions.
Viral pandemics have taken a significant toll on humanity and the world now is contending with th... more Viral pandemics have taken a significant toll on humanity and the world now is contending with the SARS-CoV-2 epidemic. Readily available economical preventive measures should be immediately explored. Xylitol has been reported to reduce the severity of viral infections as well as the severity of pneumonia, and increase the survivability of animal subjects. Since pneumonia and acute respiratory distress syndrome are potentially fatal complications of COVID-19, the present study tested the in vitro effectiveness of xylitol against SARS-CoV-2. Virus titers and LRV of SARS-CoV-2, were incubated with a single concentration of nasal spray. Toxicity was observed in the top dilution (1/10). Virus was seen below that dilution so it did not affect calculations of virus titer or LRV. After a 25-minute contact time, the nasal spray (11% Pure Xylitol, 0.85%NaCL (Saline), and 0.20% grapefruit seed extract) reduced virus from 4.2 to 1.7 log10 CCID50 per 0.1 mL, a statistically significant reductio...
Journal of applied oral science : revista FOB
Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized... more Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHA...
Probiotics and Antimicrobial Proteins, May 7, 2011
Probiotics have been widely publicized in the general press and the consumer media. Knowledge of ... more Probiotics have been widely publicized in the general press and the consumer media. Knowledge of the existence of "probiotics" is commonplace, and the effectiveness of probiotic therapy has been well reported in the medical literature. However, even though most published dental studies have reported positive results, the dental profession has not yet accepted the use of probiotic therapy as an adjunct for preventive dental care. This review article discusses published and current research into the applications of probiotics along with diagnostic testing of the oral biofilm. Probiotic therapy appears to be generally safe and effective in modifying with beneficial bacteria the oral biofilm and thereby reducing the effects of pathogenic oral bacteria. In this review, some examples of current oral probiotic research are discussed along with reference to the potential application of diagnostic testing of the oral biofilm for the presence of oral pathogens as a precursor to initiation of specific probiotic therapy. Dental professionals should be actively investigating this potentially very useful therapeutic measure for the benefit of their patients.
Journal of Dentistry For Children, 2005
The objective of this study was to evaluate the position of the mandibular lingula (ML) to provid... more The objective of this study was to evaluate the position of the mandibular lingula (ML) to provide data for inferior alveolar nerve block techniques in children. One hundred fifty-four panoramic radiographs of 7- to 10-year-old boys and girls were analyzed. Measurements were taken from the ML to the occlusal plane, and the percentile distances of the ML to ramal borders were determined. The distance between the ML and the occlusal plane showed a gradual increase, but only in the male group was it statistically significant. ML's ratio position on the ramus remained constant in all analyzed groups. In the 7-year-old group, the ML was observed above the occlusal plane in 70% of girls and 55% of boys. That percentage reached 85% of all children by age 10. The mandibular lingula's ratio position remained constant. Inferior alveolar anesthesia should be administered at least 6 mm above the occlusal plane in 7- to 8-year-old children, while 10 mm could be indicated for 9- to 10-year-old children. The mandibular lingula should be considered a reliable reference for further…
Journal of Dentistry For Children, 2003
This study evaluated the clinical efficacy of the &am... more This study evaluated the clinical efficacy of the "open sandwich" restoration for pediatric dental practice. Three pediatric dentists used a standardized preparation and restorative technique to place the restorations. The prepared tooth was etched with a phosphoric acid semigel and rinsed. A resin modified glass ionomer (Fuji II LCor Photac-Fil) was placed short of the margins and then light cured. The resin modified glass ionomer was covered with an occlusal layer of a microhybrid flowable composite (Aeliteflo or Flow-it). The same preparation for the experimental restorations was used for the control conventional amalgam (Tytin) restorations. The restorations were evaluated at 6-month intervals and ranked with a modified United States Public Heath Survey (USPHS) scale as follows: Alfa: No discernible marginal opening or stain; Beta: Slight opening of margin discernible with dental explorer, but without stain; Charlie: Open margin and stain; Delta: Recurrent caries or restoration failure. Restoration failures were categorized according to etiology, pulpal necrosis, bruxism, marginal leakage, isthmus fracture, or adhesive failure. All recalled experimental restorations, except 8, were rated as either Alpha or Beta. Six failed due to isthmus fracture and 2 due to pulpal necrosis. Fifteen restorations had delaminating of the flowable composite from the resin modified glass ionomer. The use of the "open sandwich" technique compared favorably with a similar study using adhesive amalgam restorations. The "open sandwich" technique can be successfully used in a pediatric dental practice.
American Journal of Dentistry, Aug 1, 2013
To evaluate the biochemical and microbiological characteristics of in situ biofilm formed on mate... more To evaluate the biochemical and microbiological characteristics of in situ biofilm formed on materials that release fluoride (F-) or calcium (Ca++) and phosphate (Pi). This study comprised an in situ and in vitro experiment, utilizing three materials [Auralay XF and Fuji IX GP, containing fluoride, and Aegis containing amorphous calcium phosphate (ACP)] and bovine dental enamel slabs. For the in situ: 10 volunteers wore palatal devices, each containing four material specimens or enamel slabs that were treated with 20% sucrose solution. The biofilm had pH measurements on Day 7 and the composition was analyzed on Day 8 by assessing the following: F-, Ca++, Pi and insoluble extracellular polysaccharides (EPS) concentrations, and then identification of the microbiota. For the in vitro: materials/enamel were subjected to a 7-day pH-cycling regimen to determine F, Ca++ and Pi release. The biofilm formed on F(-)-releasing materials was richer in F, Ca++ and Pi and had lower mutans streptococci counts than enamel biofilm. The biofilm on the ACP-containing material exhibited similar Ca++ and Pi concentrations to biofilm on F(-)-releasing materials. The materials showed buffering action compared with enamel. Biochemical and microbiological characteristics showed a less cariogenic biofilm on materials containing fluoride or amorphous calcium phosphate.
Pediatric dentistry, 1983
... Ira N. Moyer, DDS Roger H. Kobayashi, MD Mark L. Cannon, DDS, MS John F. Simon, DDS, MS Rober... more ... Ira N. Moyer, DDS Roger H. Kobayashi, MD Mark L. Cannon, DDS, MS John F. Simon, DDS, MS Robert O. Cooley, DDS, MS ... In one variant of this disease, a missing enzyme, adenine deaminase (ADA), leads to the accumulation of metabolic by-products which are toxic to the ...
Compendium of continuing education in dentistry (Jamesburg, N.J. : 1995), 2003
The introduction of low-shrinkage composite and no-rinse conditioners has created an opportunity ... more The introduction of low-shrinkage composite and no-rinse conditioners has created an opportunity for pediatric dentists to change their standard operative regimen. The low-shrink composite solves many of the problems that have discouraged clinicians from routinely providing posterior composites for their patients. The reduction in polymerization shrinkage decreases problems with contraction stresses, sensitivity, microleakage, recurrent caries, and negative pulpal sequelae. No-rinse conditioners simplify the process and shorten the time required for bonding procedures. Liquid polish reduces the tedious finishing previously required with resin-based composites. The combination of the easier bonding and more user-friendly composite could add a new operative technique to the pediatric dentist's armamentarium. A study of low-shrink, resin-based composite restorations was performed to determine the effectiveness of these new materials for pediatric dental practice. The restorations w...
Compendium of continuing education in dentistry (Jamesburg, N.J. : 1995), 2013
The Journal of clinical pediatric dentistry, 2013
This IRB approved clinical trial was to determine the effect of "over the counter" prob... more This IRB approved clinical trial was to determine the effect of "over the counter" probiotic supplements on the Caries Risk Test- CRT- (Ivoclar) results of the oral microflora in high caries risk children. Sixty subjects 6 to 12 years old with a caries risk assessment (CAMBRA) of moderate to high (caries prone) were evaluated by an analysis of the difference in the salivary levels of pathogenic bacteria (mutans streptococci and Lactobacilli). The subjects were randomly selected by randomizing software and assigned to two diferent Groups. Group A used PerioBalance (Lactobacilli reuteri-CFU of 200 million) lozenges for 28 days. Group B used the EvoraKids (Streptococcus uberis KJ2, Streptococcus oralis KJ3, Streptococcus rattus JH1 45, > or = 100 million) probiotics chewable tablets for 30 days. Salivary samples were collected then incubated for 48 hours for colony counting and ranking. Follow up testing with the CRT was performed after 60 days at a follow up visit. There ...
Encyclopedia of Medical Devices and Instrumentation, 2006
... 17. Sterman S, Marsden J. Silane coupling agents as integral blends in resin-filler systems. ... more ... 17. Sterman S, Marsden J. Silane coupling agents as integral blends in resin-filler systems. Mod Plastics 1963;49(11):125. ... Deliperi S, Bardwell D. An alternative method to reduce polymerization shrinkage in direct posterior composite restorations. JADA 2002;133:1387–1398. ...
Journal of Endodontics, 2008
The purpose of this study was to evaluate the subcutaneous response of rat connective tissue to l... more The purpose of this study was to evaluate the subcutaneous response of rat connective tissue to light-cure MTA and Ângelus MTA. These materials were placed in polyethylene and dentin tubes and implanted into dorsal connective tissue of Wistar rats for 30 and 60 days. The specimens were prepared to be stained with hematoxylin-eosin, Von Kossa, and without stain for polarized light and evaluated in an optic microscope. The Ângelus MTA showed a mild inflammatory response at 30 days and none at 60 days, characterized by organized connective tissue, presence of some chronic inflammatory cells, and induction of mineralized tissue formation. Light-cure MTA presented a moderate chronic inflammatory response at 30 days that decreased at 60 days but was more intense than with Ângelus MTA and without dystrophic calcifications. It was possible to conclude that light-cure MTA was similar to MTA at 60 days, but it did not stimulate mineralization.
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2010
The aim of this study was to evaluate the rat alveolar socket response to implanted polyethylene ... more The aim of this study was to evaluate the rat alveolar socket response to implanted polyethylene tubes filled with a light-cured MTA or Angelus MTA. Study design. Fifty-seven Wistar rats received an implant filled with one of the materials in the dental socket of the right central incisor. After 30, 60, and 90 days, animals were killed, and the right hemimaxillas were removed and processed to be analyzed on light microscopy. After embedding in paraffin, 6-m-thick sections were obtained and stained with hematoxylin and eosin. Qualitative and quantitative analyses of the reaction were performed. Results. It was observed that the light-cured MTA presented a similar response compared with Angelus MTA, characterized by the reduction of the chronic inflammatory response and organization of the alveolar bone close to the materials, especially at the 90th day of observation. Conclusions. It was concluded that both materials were well accepted by the alveolar tissue of rats, with the formation of mineralized tissue close to the materials. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;110:e93-e97) MATERIAL AND METHODS Fifty-seven 4-to 6-month-old male Wistar Albino rats weighing 250-280 g were used. The animals were housed in temperature-controlled rooms and received
Operative Dentistry, 2007
Clinical Relevance When considering the individual needs of patients in the clinical setting, it ... more Clinical Relevance When considering the individual needs of patients in the clinical setting, it is important to know the behavior of restorative materials in the oral environment under pH variations.