Cerebral cortical blood flow maps are reorganized in MAOB-deficient mice - PubMed (original) (raw)

Cerebral cortical blood flow maps are reorganized in MAOB-deficient mice

O U Scremin et al. Brain Res. 1999.

Abstract

Cerebral cortical blood flow (CBF) was measured autoradiographically in conscious mice without the monoamine oxidase B (MAOB) gene (KO, n=11) and the corresponding wild-type animals (WILD, n=11). Subgroups of animals of each genotype received a continuous intravenous infusion over 30 min of phenylethylamine (PEA), an endogenous substrate of MAOB, (8 nmol g-1 min-1 in normal saline at a volume rate of 0.11 microl g-1 min-1) or saline at the same volume rate. Maps of relative CBF distribution showed predominance of midline motor and sensory area CBF in KO mice over WILD mice that received saline. PEA enhanced CBF in lateral frontal and piriform cortex in both KO and WILD mice. These changes may reflect a differential activation due to chronic and acute PEA elevations on motor and olfactory function, as well as on the anxiogenic effects of this amine. In addition to its effects on regional CBF distribution, PEA decreased CBF globally in KO mice (range -31% to -41% decrease from control levels) with a lesser effect in WILD mice. It is concluded that MAOB may normally regulate CBF distribution and its response to blood PEA.

Copyright 1999 Elsevier Science B.V.

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Figures

Fig. 1

Fig. 1

Bars represent _Z_-score differences between KO mice and WILD mice receiving saline (top figure), and KO mice receiving saline or PEA (middle figure). Negative values are shown with bars projecting below the zero plane (black top). Coronal slices are numbered from rostral to caudal, with distance to bregma as follows (positive values being rostral to this landmark): (1) 1.54 mm, (2) 1.18 mm, (3) 0.74 mm, (4) 0.26 mm, (5) −0.34 mm, (6) −0.82 mm, (7) −1.34 mm, (8) −1.82 mm, (9) −2.46 mm, (10) −2.92 mm, (11) −3.64 mm. The cerebral cortical locations corresponding to each measurement are numbered from dorsal midline to lateral and ventral. These are shown in the bottom panel. Abbreviations: AI = agranular insular, AU = auditory, BF = barrel field, C1 = cingulate, CA = amygdaloid, EC = ectorhinal, EN = entorhinal, GI = granular insular, M1 = primary motor, M2 = secondary motor, PI = piriform, PR = perirhinal, RS = retrosplenial, S1 = primary somatosensory, S2 = secondary somatosensory, TA = transition between barrel field and secondary somatosensory, TB = transition between primary somatosensory and primary motor, TC = transition between secondary somatosensory and perirhinal, TD = transition between cingulate and motor, V1 = primary visual, V2 = secondary visual. Outlined areas are those listed in Table 1.

Fig. 2

Fig. 2

Canonical plot for the two first canonical variables providing discrimination between the relative CBF (_Z_-scores) of KO and WILD mice receiving saline, and by infusion type (PEA or saline) for both KO and WILD mice. For details see text.

Fig. 3

Fig. 3

Two representative iodo-14C-antipyrine autoradiographs of slice 4 and slice 6 are shown in the left panels. Regions sampled are indicated by numerals. The corpus callosum and anterior commissure (slice 4) and the corpus callosum and hippocampal commissure (slice 6) are outlined as reference. The bar graphs (right panels) indicate group means and standard errors of CBF of the regions (numerals), as well as the slice means (ALL) in units of ml g−1 min−1. Abbreviations: WILD + S = WILD mice receiving saline; WILD + PEA = WILD mice receiving PEA; KO + S = KO mice receiving saline; KO + PEA = KO mice receiving PEA. * Statistically significant when compared to WILD + S, P < 0.05, Bonferroni correction for three contrasts.

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References

    1. B A. Bailey, S R. Philips, A A. Boulton. In vivo release of endogenous dopamine, 5-hydroxytryptamine and some of their metabolites from rat caudate nucleus by phenylethylamine. Neurochem. Res. 1987;12:173–178. - PubMed
    1. Boulton AA. Some aspects of basic psychopharmacology: the trace amines, Prog. Neuro-Psychopharmacol. Biol. Psychiatry. 1982;6:563–570. - PubMed
    1. Bussone G, Giovannini P, Boiardi A, Boeri R. A study of the activity of platelet monoamine oxidase in patients with migraine headaches or with ‘cluster headaches’. Eur. Neurol. 1977;15:157–162. - PubMed
    1. Caramona MM, Cotrim MD, Ribeiro CF, Macedo T. Monoamine oxidase activity in blood platelets of migraine patients. J. Neural Transm. Suppl. 1990;32:161–164. - PubMed
    1. Clark C, Carson R, Kessler R, Margolin R, Buchsbaum M, DeLisi L, King C, Cohen R. Alternative statistical models for the examination of clinical positron emission tomography/fluorodeoxyglucose data. J. Cereb. Blood Flow Metab. 1985;5:142–150. - PubMed

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