Neosynthesis and activation of Rho by Escherichia coli cytotoxic necrotizing factor (CNF1) reverse cytopathic effects of ADP-ribosylated Rho - PubMed (original) (raw)
. 1999 Sep 24;274(39):27407-14.
doi: 10.1074/jbc.274.39.27407.
Affiliations
- PMID: 10488072
- DOI: 10.1074/jbc.274.39.27407
Free article
Neosynthesis and activation of Rho by Escherichia coli cytotoxic necrotizing factor (CNF1) reverse cytopathic effects of ADP-ribosylated Rho
H Barth et al. J Biol Chem. 1999.
Free article
Abstract
Clostridium botulinum exoenzyme C3 inactivates the small GTPase Rho by ADP-ribosylation. We used a C3 fusion toxin (C2IN-C3) with high cell accessibility to study the kinetics of Rho inactivation by ADP-ribosylation. In primary cultures of rat astroglial cells and Chinese hamster ovary cells, C2IN-C3 induced the complete ADP-ribosylation of RhoA and concomitantly the disassembly of stress fibers within 3 h. Removal of C2IN-C3 from the medium caused the recovery of stress fibers and normal cell morphology within 4 h. The regeneration was preceded by the appearance of non-ADP-ribosylated RhoA. Recovery of cell morphology was blocked by the proteasome inhibitor lactacystin and by the translation inhibitors cycloheximide and puromycin, indicating that intracellular degradation of the C3 fusion toxin and the neosynthesis of Rho were required for reversal of cell morphology. Escherichia coli cytotoxic necrotizing factor CNF1, which activates Rho by deamidation of Gln(63), caused reconstitution of stress fibers and cell morphology in C2IN-C3-treated cells within 30-60 min. The effect of CNF1 was independent of RhoA neosynthesis and occurred in the presence of completely ADP-ribosylated RhoA. The data show three novel findings; 1) the cytopathic effects of ADP-ribosylation of Rho are rapidly reversed by neosynthesis of Rho, 2) CNF1-induced deamidation activates ADP-ribosylated Rho, and 3) inhibition of Rho activation but not inhibition of Rho-effector interaction is a major mechanism underlying inhibition of cellular functions of Rho by ADP-ribosylation.
Similar articles
- Gln 63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor-1.
Schmidt G, Sehr P, Wilm M, Selzer J, Mann M, Aktories K. Schmidt G, et al. Nature. 1997 Jun 12;387(6634):725-9. doi: 10.1038/42735. Nature. 1997. PMID: 9192900 - Escherichia coli cytotoxic necrotizing factor 1: evidence for induction of actin assembly by constitutive activation of the p21 Rho GTPase.
Fiorentini C, Donelli G, Matarrese P, Fabbri A, Paradisi S, Boquet P. Fiorentini C, et al. Infect Immun. 1995 Oct;63(10):3936-44. doi: 10.1128/iai.63.10.3936-3944.1995. Infect Immun. 1995. PMID: 7558302 Free PMC article. - Cytotoxic necrotizing factor 1 from Escherichia coli: a toxin with a new intracellular activity for eukaryotic cells.
Boquet P. Boquet P. Folia Microbiol (Praha). 1998;43(3):285-9. doi: 10.1007/BF02818614. Folia Microbiol (Praha). 1998. PMID: 9717256 Review. - Bacterial toxins and the Rho GTP-binding protein: what microbes teach us about cell regulation.
Fiorentini C, Gauthier M, Donelli G, Boquet P. Fiorentini C, et al. Cell Death Differ. 1998 Sep;5(9):720-8. doi: 10.1038/sj.cdd.4400412. Cell Death Differ. 1998. PMID: 10200530 Review.
Cited by
- Role of Host Small GTPases in Apicomplexan Parasite Infection.
Paone S, Olivieri A. Paone S, et al. Microorganisms. 2022 Jul 7;10(7):1370. doi: 10.3390/microorganisms10071370. Microorganisms. 2022. PMID: 35889089 Free PMC article. Review. - Novel bacterial ADP-ribosylating toxins: structure and function.
Simon NC, Aktories K, Barbieri JT. Simon NC, et al. Nat Rev Microbiol. 2014 Sep;12(9):599-611. doi: 10.1038/nrmicro3310. Epub 2014 Jul 14. Nat Rev Microbiol. 2014. PMID: 25023120 Free PMC article. Review. - Targeting of the actin cytoskeleton by insecticidal toxins from Photorhabdus luminescens.
Lang AE, Schmidt G, Sheets JJ, Aktories K. Lang AE, et al. Naunyn Schmiedebergs Arch Pharmacol. 2011 Mar;383(3):227-35. doi: 10.1007/s00210-010-0579-5. Epub 2010 Nov 12. Naunyn Schmiedebergs Arch Pharmacol. 2011. PMID: 21072628 Review. - Characterization of the enzymatic component of the ADP-ribosyltransferase toxin CDTa from Clostridium difficile.
Gülke I, Pfeifer G, Liese J, Fritz M, Hofmann F, Aktories K, Barth H. Gülke I, et al. Infect Immun. 2001 Oct;69(10):6004-11. doi: 10.1128/IAI.69.10.6004-6011.2001. Infect Immun. 2001. PMID: 11553537 Free PMC article. - Cytotoxic necrotizing factor 1 of Escherichia coli stimulates Rho/Rho-kinase-dependent myosin light-chain phosphorylation without inactivating myosin light-chain phosphatase in endothelial cells.
Essler M, Linder S, Schell B, Hüfner K, Wiedemann A, Randhahn K, Staddon JM, Aepfelbacher M. Essler M, et al. Infect Immun. 2003 Sep;71(9):5188-93. doi: 10.1128/IAI.71.9.5188-5193.2003. Infect Immun. 2003. PMID: 12933863 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous