Hexokinase in astrocytes: kinetic and regulatory properties - PubMed (original) (raw)
Hexokinase in astrocytes: kinetic and regulatory properties
J C Lai et al. Metab Brain Dis. 1999 Jun.
Abstract
Hexokinase (HK, EC 2.7.1.1) is a key enzyme in the control of brain glucose metabolism. The regulatory role of HK in different neural cell types has not been elucidated. In this study we determined some kinetic and regulatory properties of HK in mouse cerebrocortical astrocytes in primary culture. Astroglial HK showed an absolute requirement for Mg-ATP and D-glucose. The pH optimum of HK was between 7.4 and 8.0. For astroglial HK, the Km for Mg-ATP was approximately 208 microM and Vmax approximately 35.4 mU/mg protein. At levels higher than 0.2 mM, D-glucose-1,6-bisphosphate, a known regulator of glycolysis, inhibited astroglial HK in a concentration-dependent manner, with an IC50 of approximately 0.4 mM; at 1.2 mM, it almost completely inhibited HK activity. The results obtained for astroglial HK are compatible with those reported for the highly purified preparations of brain HK. These data are of direct relevance to the assessment of glycolytic flux and its regulation in astrocytes.
Similar articles
- Enzymatic properties of overexpressed human hexokinase fragments.
Bianchi M, Serafini G, Bartolucci E, Giammarini C, Magnani M. Bianchi M, et al. Mol Cell Biochem. 1998 Dec;189(1-2):185-93. doi: 10.1023/a:1006962217495. Mol Cell Biochem. 1998. PMID: 9879670 - Retinal hexokinase: kinetic properties and the effect of cyclic 3',5'-adenosine monophosphate.
Liu SS, Witkovsky P, Yang CY. Liu SS, et al. J Neurochem. 1983 Dec;41(6):1694-701. doi: 10.1111/j.1471-4159.1983.tb00882.x. J Neurochem. 1983. PMID: 6315879 - Mitochondria-Bound Hexokinase (mt-HK) Activity Differ in Cortical and Hypothalamic Synaptosomes: Differential Role of mt-HK in H2O2 Depuration.
Cavalcanti-de-Albuquerque JP, de Souza Ferreira E, de Carvalho DP, Galina A. Cavalcanti-de-Albuquerque JP, et al. Mol Neurobiol. 2018 Jul;55(7):5889-5900. doi: 10.1007/s12035-017-0807-9. Epub 2017 Nov 8. Mol Neurobiol. 2018. PMID: 29119535 - [Targeting the gene of glucose metabolism for the treatment of advanced gliomas].
Oudard S, Miccoli L, Dutrillaux B, Poupon MF. Oudard S, et al. Bull Cancer. 1998 Jul;85(7):622-6. Bull Cancer. 1998. PMID: 9752269 Review. French. - Isozymes of plant hexokinase: occurrence, properties and functions.
Claeyssen E, Rivoal J. Claeyssen E, et al. Phytochemistry. 2007 Mar;68(6):709-31. doi: 10.1016/j.phytochem.2006.12.001. Epub 2007 Jan 17. Phytochemistry. 2007. PMID: 17234224 Review.
Cited by
- Integrated lipidomic and transcriptomic analysis reveals diacylglycerol accumulation in olive of Longnan (China).
Hu W, Ma J, Zhang H, Miu X, Miao X, Deng Y. Hu W, et al. PeerJ. 2023 Aug 11;11:e15724. doi: 10.7717/peerj.15724. eCollection 2023. PeerJ. 2023. PMID: 37583911 Free PMC article. - The Role of Dual-Phase FDG PET/CT in the Diagnosis and Follow-Up of Brain Tumors.
Özütemiz C, Neil EC, Tanwar M, Rubin NT, Ozturk K, Cayci Z. Özütemiz C, et al. AJR Am J Roentgenol. 2020 Oct;215(4):985-996. doi: 10.2214/AJR.19.22571. Epub 2020 Aug 25. AJR Am J Roentgenol. 2020. PMID: 32841063 Free PMC article. - How glycogen sustains brain function: A plausible allosteric signaling pathway mediated by glucose phosphates.
DiNuzzo M. DiNuzzo M. J Cereb Blood Flow Metab. 2019 Aug;39(8):1452-1459. doi: 10.1177/0271678X19856713. Epub 2019 Jun 17. J Cereb Blood Flow Metab. 2019. PMID: 31208240 Free PMC article. Review. - Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism.
Lundgaard I, Li B, Xie L, Kang H, Sanggaard S, Haswell JD, Sun W, Goldman S, Blekot S, Nielsen M, Takano T, Deane R, Nedergaard M. Lundgaard I, et al. Nat Commun. 2015 Apr 23;6:6807. doi: 10.1038/ncomms7807. Nat Commun. 2015. PMID: 25904018 Free PMC article. - Diffusion of D-glucose measured in the cytosol of a single astrocyte.
Kreft M, Lukšič M, Zorec TM, Prebil M, Zorec R. Kreft M, et al. Cell Mol Life Sci. 2013 Apr;70(8):1483-92. doi: 10.1007/s00018-012-1219-7. Epub 2012 Dec 8. Cell Mol Life Sci. 2013. PMID: 23224430 Free PMC article.
References
- Brain Res. 1992 Sep 25;591(2):277-82 - PubMed
- J Neurochem. 1985 Jun;44(6):1741-6 - PubMed
- Comp Biochem Physiol C. 1985;80(2):291-4 - PubMed
- J Biol Chem. 1966 Aug 10;241(15):3546-60 - PubMed
- J Neurochem. 1977 Dec;29(6):1039-51 - PubMed
Publication types
MeSH terms
Substances
Grants and funding
- DK27121/DK/NIDDK NIH HHS/United States
- P01 HD032573/HD/NICHD NIH HHS/United States
- NS34813/NS/NINDS NIH HHS/United States
- R01 NS034813/NS/NINDS NIH HHS/United States
- R01 DK027121/DK/NIDDK NIH HHS/United States
- HD32573/HD/NICHD NIH HHS/United States
LinkOut - more resources
Full Text Sources
Research Materials