Expression of pTalpha mRNA in a committed dendritic cell precursor in the human thymus - PubMed (original) (raw)

Comparative Study

. 1999 Oct 15;94(8):2647-57.

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• PMID: 10515868

Free article

Comparative Study

Expression of pTalpha mRNA in a committed dendritic cell precursor in the human thymus

P C Res et al. Blood. 1999.

Free article

Abstract

We have characterized dendritic cell precursors (pre-DC) in the human thymus. These CD1a(-)CD3(-)CD4(+)CD8(-) cells express high levels of interleukin-3Ralpha (IL-3Ralpha) on the membrane and are able to develop into mature DC upon culture with IL-3 and CD40 ligation. The DC precursors are predominantly located in the thymic medulla. Interestingly, the pre-DC express pTalpha mRNA, which is also present in CD1a(+)CD3(-)CD4(+)CD8(-) pre-T cells. Yet, the pre-DC lack expression of recombination activating gene-1 mRNA and fail to develop into T cells in appropriate assays. The thymic pre-DC are very similar to the recently characterized pre-DC found in the T cell areas of the tonsil, and it is suggested that these pre-DC populations are of lymphoid origin.

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