Isolation and characterization of promoter regions from Streptococcus gordonii CH1 - PubMed (original) (raw)

. 1999 Dec;39(6):321-0326.

doi: 10.1007/s002849900466.

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Isolation and characterization of promoter regions from Streptococcus gordonii CH1

A J Vriesema et al. Curr Microbiol. 1999 Dec.

Abstract

We aimed to identify transcription signal sequences from Streptococcus gordonii strain CH1 by random chromosomal cloning. Five genomic fragments from a Sau3A digest, which constitutively activated transcription of a promoterless spectinomycin resistance gene in this strain, were isolated and characterized. Additionally, one promoter fragment was isolated that was specifically activated under iron-limiting conditions. A sequence motif with similarity to the consensus for Fur-binding regulatory DNA sequences (Fur box) in Escherichia coli was detected within the putative promoter region. The open reading frame downstream of this region possibly encodes a transmembrane protein involved in iron uptake.

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