Mice lacking Smad3 show accelerated wound healing and an impaired local inflammatory response - PubMed (original) (raw)

Mice lacking Smad3 show accelerated wound healing and an impaired local inflammatory response

G S Ashcroft et al. Nat Cell Biol. 1999 Sep.

Abstract

The generation of animals lacking SMAD proteins, which transduce signals from transforming growth factor-beta (TGF-beta), has made it possible to explore the contribution of the SMAD proteins to TGF-beta activity in vivo. Here we report that, in contrast to predictions made on the basis of the ability of exogenous TGF-beta to improve wound healing, Smad3-null (Smad3ex8/ex8) mice paradoxically show accelerated cutaneous wound healing compared with wild-type mice, characterized by an increased rate of re-epithelialization and significantly reduced local infiltration of monocytes. Smad3ex8/ex8 keratinocytes show altered patterns of growth and migration, and Smad3ex8/ex8 monocytes exhibit a selectively blunted chemotactic response to TGF-beta. These data are, to our knowledge, the first to implicate Smad3 in specific pathways of tissue repair and in the modulation of keratinocyte and monocyte function in vivo.

PubMed Disclaimer

Comment in

• Wounding Smad.
  Massagué J. Massagué J. Nat Cell Biol. 1999 Sep;1(5):E117-9. doi: 10.1038/12944. Nat Cell Biol. 1999. PMID: 10559949 No abstract available.

Similar articles

• Wounding Smad.
  Massagué J. Massagué J. Nat Cell Biol. 1999 Sep;1(5):E117-9. doi: 10.1038/12944. Nat Cell Biol. 1999. PMID: 10559949 No abstract available.
• Selective reduction of fibrotic markers in repairing corneas of mice deficient in Smad3.
  Stramer BM, Austin JS, Roberts AB, Fini ME. Stramer BM, et al. J Cell Physiol. 2005 Apr;203(1):226-32. doi: 10.1002/jcp.20215. J Cell Physiol. 2005. PMID: 15521071
• Essential role for Smad3 in regulating MCP-1 expression and vascular inflammation.
  Feinberg MW, Shimizu K, Lebedeva M, Haspel R, Takayama K, Chen Z, Frederick JP, Wang XF, Simon DI, Libby P, Mitchell RN, Jain MK. Feinberg MW, et al. Circ Res. 2004 Mar 19;94(5):601-8. doi: 10.1161/01.RES.0000119170.70818.4F. Epub 2004 Jan 29. Circ Res. 2004. PMID: 14752027
• Loss of Smad3 modulates wound healing.
  Ashcroft GS, Roberts AB. Ashcroft GS, et al. Cytokine Growth Factor Rev. 2000 Mar-Jun;11(1-2):125-31. doi: 10.1016/s1359-6101(99)00036-2. Cytokine Growth Factor Rev. 2000. PMID: 10708960 Review.
• [Transforming growth factor-betas: smad signaling and roles in physiopathology].
  Javelaud D, Mauviel A. Javelaud D, et al. Pathol Biol (Paris). 2004 Feb;52(1):50-4. doi: 10.1016/j.patbio.2003.10.002. Pathol Biol (Paris). 2004. PMID: 14761714 Review. French.

Cited by

• Using widely targeted metabolomics profiling to explore differences in constituents of three Bletilla species.
  Xu W, Huang J, Wang P, Yang Y, Fu S, Ying Z, Zhou Z. Xu W, et al. Sci Rep. 2024 Oct 12;14(1):23873. doi: 10.1038/s41598-024-74204-y. Sci Rep. 2024. PMID: 39396087 Free PMC article.
• TGF-β Isoforms and Local Environments Greatly Modulate Biological Nature of Human Retinal Pigment Epithelium Cells.
  Nishikiori N, Sato T, Ogawa T, Higashide M, Umetsu A, Suzuki S, Furuhashi M, Ohguro H, Watanabe M. Nishikiori N, et al. Bioengineering (Basel). 2024 Jun 7;11(6):581. doi: 10.3390/bioengineering11060581. Bioengineering (Basel). 2024. PMID: 38927817 Free PMC article.
• Advances and Challenges in Targeting TGF-β Isoforms for Therapeutic Intervention of Cancer: A Mechanism-Based Perspective.
  Danielpour D. Danielpour D. Pharmaceuticals (Basel). 2024 Apr 20;17(4):533. doi: 10.3390/ph17040533. Pharmaceuticals (Basel). 2024. PMID: 38675493 Free PMC article. Review.
• TGF-β signaling in health, disease, and therapeutics.
  Deng Z, Fan T, Xiao C, Tian H, Zheng Y, Li C, He J. Deng Z, et al. Signal Transduct Target Ther. 2024 Mar 22;9(1):61. doi: 10.1038/s41392-024-01764-w. Signal Transduct Target Ther. 2024. PMID: 38514615 Free PMC article. Review.
• Antiviral drugs prolong survival in murine recessive dystrophic epidermolysis bullosa.
  Tartaglia G, Fuentes I, Patel N, Varughese A, Israel LE, Park PH, Alexander MH, Poojan S, Cao Q, Solomon B, Padron ZM, Dyer JA, Mellerio JE, McGrath JA, Palisson F, Salas-Alanis J, Han L, South AP. Tartaglia G, et al. EMBO Mol Med. 2024 Apr;16(4):870-884. doi: 10.1038/s44321-024-00048-8. Epub 2024 Mar 10. EMBO Mol Med. 2024. PMID: 38462666 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • GlyGen glycoinformatics resource
  • Mouse Genome Informatics (MGI)