Human CED-6 encodes a functional homologue of the Caenorhabditis elegans engulfment protein CED-6 - PubMed (original) (raw)

Comparative Study

. 1999 Nov 18;9(22):1347-50.

doi: 10.1016/s0960-9822(00)80061-5.

Affiliations

• PMID: 10574771
• DOI: 10.1016/s0960-9822(00)80061-5

Free article

Comparative Study

Human CED-6 encodes a functional homologue of the Caenorhabditis elegans engulfment protein CED-6

Q A Liu et al. Curr Biol. 1999.

Free article

Abstract

The rapid engulfment of apoptotic cells is a specialized innate immune response used by organisms to remove apoptotic cells. In mammals, several receptors that recognize apoptotic cells have been identified; molecules that transduce signals from these receptors to downstream cytoskeleton molecules have not been found, however [1] [2] [3]. Our previous analysis of the engulfment gene ced-6 in Caenorhabditis elegans has suggested that CED-6 is an adaptor protein that participates in a signal transduction pathway that mediates the specific recognition and engulfment of apoptotic cells [1]. Here, we describe our isolation and characterization of a human cDNA encoding a protein, hCED-6, with strong sequence similarity to C. elegans CED-6. As is the case with the worm protein, hCED-6 contains a phosphotyrosine-binding (PTB) domain and potential Src-homology domain 3 (SH3) binding sites. Both CED-6 and hCED-6 contain a predicted coiled-coil domain in the middle region. The hCED-6 protein lacks the extended carboxyl terminus found in worm CED-6; this carboxy-terminal extension appears not to be essential for CED-6 function in C. elegans, however. Overexpression of hCED-6 rescues the engulfment defect of ced-6 mutants in C. elegans significantly, suggesting that hCED-6 is a functional homologue of C. elegans CED-6. Human ced-6 is expressed widely in most human tissues. Thus, CED-6, and the CED-6 signal transduction pathway, might be conserved from C. elegans to humans and are present in most, if not all, human tissues.

PubMed Disclaimer

Similar articles

• The human homologue of Caenorhabditis elegans CED-6 specifically promotes phagocytosis of apoptotic cells.
  Smits E, Van Criekinge W, Plaetinck G, Bogaert T. Smits E, et al. Curr Biol. 1999 Nov 18;9(22):1351-4. doi: 10.1016/s0960-9822(00)80062-7. Curr Biol. 1999. PMID: 10574763
• Candidate adaptor protein CED-6 promotes the engulfment of apoptotic cells in C. elegans.
  Liu QA, Hengartner MO. Liu QA, et al. Cell. 1998 Jun 12;93(6):961-72. doi: 10.1016/s0092-8674(00)81202-7. Cell. 1998. PMID: 9635426
• Identification of a mouse orthologue of the CED-6 gene of Caenorhabditis elegans.
  Banerjee H, Hawkins Z, Johnson T, Eley S, Alikhan A, Mcdaniel M, Singh I, Raymond J. Banerjee H, et al. Plasmid. 2003 Jan;49(1):30-3. doi: 10.1016/s0147-619x(02)00106-3. Plasmid. 2003. PMID: 12583998
• The engulfment process of programmed cell death in caenorhabditis elegans.
  Reddien PW, Horvitz HR. Reddien PW, et al. Annu Rev Cell Dev Biol. 2004;20:193-221. doi: 10.1146/annurev.cellbio.20.022003.114619. Annu Rev Cell Dev Biol. 2004. PMID: 15473839 Review.
• STAR, a gene family involved in signal transduction and activation of RNA.
  Vernet C, Artzt K. Vernet C, et al. Trends Genet. 1997 Dec;13(12):479-84. doi: 10.1016/s0168-9525(97)01269-9. Trends Genet. 1997. PMID: 9433137 Review.

Cited by

• Programmed Cell Death During Caenorhabditis elegans Development.
  Conradt B, Wu YC, Xue D. Conradt B, et al. Genetics. 2016 Aug;203(4):1533-62. doi: 10.1534/genetics.115.186247. Genetics. 2016. PMID: 27516615 Free PMC article.
• Cooperation between engulfment receptors: the case of ABCA1 and MEGF10.
  Hamon Y, Trompier D, Ma Z, Venegas V, Pophillat M, Mignotte V, Zhou Z, Chimini G. Hamon Y, et al. PLoS One. 2006 Dec 27;1(1):e120. doi: 10.1371/journal.pone.0000120. PLoS One. 2006. PMID: 17205124 Free PMC article.
• Integrin α PAT-2/CDC-42 signaling is required for muscle-mediated clearance of apoptotic cells in Caenorhabditis elegans.
  Hsieh HH, Hsu TY, Jiang HS, Wu YC. Hsieh HH, et al. PLoS Genet. 2012;8(5):e1002663. doi: 10.1371/journal.pgen.1002663. Epub 2012 May 17. PLoS Genet. 2012. PMID: 22615577 Free PMC article.
• Apoptotic engulfment pathway and schizophrenia.
  Chen X, Sun C, Chen Q, O'Neill FA, Walsh D, Fanous AH, Chowdari KV, Nimgaonkar VL, Scott A, Schwab SG, Wildenauer DB, Che R, Tang W, Shi Y, He L, Luo XJ, Su B, Edwards TL, Zhao Z, Kendler KS. Chen X, et al. PLoS One. 2009 Sep 1;4(9):e6875. doi: 10.1371/journal.pone.0006875. PLoS One. 2009. PMID: 19721717 Free PMC article.
• A Study to Investigate the Role of GULP/ CED 6 Gene in "Eat Me" Signaling in Cellular Efferocytosis and Immunosurveillance.
  Banerjee H, Bazemore B, Barfield A, Crummity D, Krauss C, Payne G, Rousch J, Manglik V. Banerjee H, et al. Immunol Disord Immunother. 2016 Dec;1(3):109. Epub 2016 Nov 30. Immunol Disord Immunother. 2016. PMID: 28286882 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science
  • Zurich Open Access Repository and Archive

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • GlyGen glycoinformatics resource

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal