Cyclin F regulates the nuclear localization of cyclin B1 through a cyclin-cyclin interaction - PubMed (original) (raw)

Cyclin F regulates the nuclear localization of cyclin B1 through a cyclin-cyclin interaction

M Kong et al. EMBO J. 2000.

Abstract

The key regulator of G(2)-M transition of the cell cycle is M-phase promoting factor (MPF), a complex composed of cdc2 and a B-type cyclin. Cyclin B1 nuclear localization involves phosphorylation within a region called the cytoplasmic retention signal, which also contains a nuclear export signal. The mechanism of MPF nuclear localization remains unclear since it contains no functional nuclear localization signal (NLS). We exploited the yeast two-hybrid screen to find protein(s) potentially mediating localization of cyclin B1 and identified a novel interaction between cyclin B1 and cyclin F. We found that cdc2, cyclin B1 and cyclin F form a complex that exhibits histone H1 kinase activity. Cyclin B1 and cyclin F also colocalize through immunofluorescence studies. Additionally, deletion analysis revealed that each putative NLS of cyclin F is functional. Taken together, the data suggest that the NLS regions of cyclin F regulate cyclin B1 localization to the nucleus. The interaction between cyclin B1 and cyclin F represents the first example of direct cyclin-cyclin binding, and elucidates a novel mechanism that regulates MPF localization and function.

PubMed Disclaimer

Figures

Fig. 1. Schematic representation of Xenopus cyclin B1-derived fusion proteins. The single and double CRS derivatives of cyclin B1 (aa 78–127) contained either all Ala or Glu mutations at residues 94, 96, 101 and 113. The NLS–B1 derivatives contained a nuclear localization signal appended to the N–terminus of cyclin B1. The CRS-B1 derivatives contained an additional CRS region appended to full-length cyclin B1.

Fig. 2. Human cyclin B1 and cyclin F constructs. (A) The CRS region of human cyclin B1 was synthesized with the Ser residues at 126, 128, 133 and 147 all mutated to either Ala or Glu. (B) Myristylated human cyclin F (top), ΔNLS1/2-cyclin F (middle) and cyclin F (bottom) are shown. The 188 aa region of mouse cyclin F that interacted with the CRS of cyclin B1 corresponding to human cyclin F is indicated at the bottom.

Fig. 3. Expression of cyclin F RNA during Xenopus development. Total RNA isolated from stage VI oocytes or staged embryos was isolated and analyzed by RT–PCR. RNA (200 ng) was assayed for either the presence of cyclin F (upper panel) or, as an internal control, c–src RNA (lower panel).

Fig. 4. In vitro binding of GST–cyclin F and Xenopus cyclin B1 fusion proteins. (A) Xenopus cyclin B1 derivatives were translated in reticulocyte lysates (lanes 1, 2 and 3) and then incubated with GST protein alone (lanes 4, 5 and 6) or with bacterially expressed GST–cyclin F (lanes 7, 8 and 9) bound to glutathione–agarose beads. The samples were analyzed by 7.5% SDS–PAGE and immunoblotted with mAb P5D4 directed against the epitope-tag VSV-G on each cyclin B1 derivative. (B) The membrane was re-probed with α–GST antibody, indicating the presence of GST protein (lanes 4, 5 and 6) and GST–cyclin F (lanes 7, 8 and 9). Molecular mass markers in kilodaltons are indicated.

Fig. 5. cdc2, cyclin B1 and cyclin F form a complex. Cyclin F was transfected into 293T cells and then immunoprecipitated with α–cdc2 antibody. The samples were analyzed by 10% SDS–PAGE followed by immunoblotting with α–cyclin F antibody (upper panel) and α–cyclin B1 (lower panel). The lower band in the immunoprecipitated samples in lanes 3 and 4 of the lower panel represents the IgG band from the α–cdc2 antibody. Molecular mass markers are indicated.

Fig. 6. Interaction of endogenous cyclin F with endogenous cyclin B1. (A) HeLa cells were synchronized by double-thymidine block to G2–M phase, and the lysate was immunoprecipitated with α–cyclin B1 antibody (lane 2) or α–cyclin B1 plus α–cyclin B1 blocking peptide (lane 3). Samples were analyzed by 10% SDS–PAGE followed by immunoblotting with α–cyclin F antibody (upper panel) and α–cyclin B1 antibody (lower panel). The lysate served as a positive control (lane 1). (B) Synchronized HeLa cells in G2–M were lysed and immunoprecipitated with α–cdc2 antibody (lane 2). Analysis by 10% SDS–PAGE followed by immunoblotting revealed the presence of cyclin F (upper panel), cyclin B1 (middle panel) and cdc2 (lower panel). The lysate served as a positive control (lane 1). The lower band in the cyclin B1 immunoblot of the α–cdc2 immunoprecipitate (lane 2) is due to the IgG band of the α–cdc2 antibody. (C) HeLa cells were lysed and immunoprecipitated with α–cyclin F (lane 1), α–cyclin F plus α–cyclin F blocking peptide (lane 2), or α–cdc2 antibody (lane 3). Samples were then assayed for histone H1 kinase activity. Molecular mass markers in all panels are indicated.

Fig. 7. (A) Individual localization of CRS-cyclin B1 and cyclin F in COS–1 cells synchronized in G2–M phase. Panels a and c are CRSAla-B1Ala and CRSGlu-B1Glu, respectively, fixed and stained with mAb P5D4 (green). Panel e shows cyclin F fixed and stained with α–cyclin F antibody (red). Hoechst dye 33342 reveals the nucleus in panels b, d and f (blue). (B) Colocalization of CRS-cyclin B1 and cyclin F. The CRSAla-B1Ala and cyclin F cotransfection (top row) shows that the cyclin B1 Ala mutant (green) and cyclin F (red) colocalize (yellow) in the cytoplasm. The CRSGlu-B1Glu and cyclin F cotransfection (bottom row) indicates that the cyclin B1 Glu mutant (green) and cyclin F (red) also colocalize (yellow), but in the nucleus.

Fig. 8. (A) Individual localization of NLS–B1 and myr-cyclin F in COS–1 cells synchronized in G2–M phase. Panels a and c are NLS–B1 derivatives stained with mAb P5D4 (green). Panel e is myristylated cyclin F stained with α–cyclin F antibody (red). Hoechst dye shows the nucleus in panels b, d and f (blue). (B) Colocalization of NLS–B1 and myr-cyclin F. The NLS–B1Ala and myr-cyclin F cotransfection (top row) shows that the NLS–B1Ala mutant (green) and myr-cyclin F (red) colocalize (yellow). The NLS–B1Glu and myr-cyclin F cotransfection indicates that the NLS–B1Glu mutant (green) and myr-cyclin F (red) colocalize (yellow).

Fig. 9. ΔNLS1/2-cyclin F localizes to the cytoplasm and retains NLS–B1Glu in the cytoplasm. (A) ΔNLS1/2-cyclin F transfected cells were fixed and stained with an antibody directed against the epitope-tag of cyclin F (panel a, red). The nucleus is seen with Hoechst dye (panel b, blue) and indicates that ΔNLS1/2-cyclin F is completely excluded from the nucleus. (B) NLS–B1Glu (panel c, green) is cotransfected with ΔNLS1/2-cyclin F (panel d, red) and they colocalize (panel e, yellow).

Fig. 10. Model of cyclin B1–cyclin F colocalization. (A) CRSAla-B1Ala and cyclin F colocalize in the cytoplasm, undergo nuclear transport, and then re-localize to the cytoplasm due to the functional NES of CRSAla-B1Ala. (B) CRSGlu-B1Glu and cyclin F interact in the cytoplasm and then colocalize to the nucleus. The complex is then retained in the nucleus due to the inactive NES of CRSGlu-B1Glu and properly localized to trigger the onset of mitosis. (C) Upper panel: unphosphorylated cyclin B1 and cyclin F interact in the cytoplasm and enter the nucleus. Re-localization to the cytoplasm may then occur via the active NES of cyclin B1. Lower panel: phosphorylated cyclin B1 and cyclin F colocalize from the cytoplasm to the nucleus. MPF is active and ready to initiate mitosis. In each panel, cdc2 is complexed to cyclin B1, although it is not represented.

Cited by

Comparative molecular and functional analyses of the tobacco cyclin-dependent kinase inhibitor NtKIS1a and its spliced variant NtKIS1b.
Jasinski S, Perennes C, Bergounioux C, Glab N. Jasinski S, et al. Plant Physiol. 2002 Dec;130(4):1871-82. doi: 10.1104/pp.008573. Plant Physiol. 2002. PMID: 12481070 Free PMC article.
Low cyclin F expression in hepatocellular carcinoma associates with poor differentiation and unfavorable prognosis.
Fu J, Qiu H, Cai M, Pan Y, Cao Y, Liu L, Yun J, Zhang CZ. Fu J, et al. Cancer Sci. 2013 Apr;104(4):508-15. doi: 10.1111/cas.12100. Epub 2013 Feb 17. Cancer Sci. 2013. PMID: 23305207 Free PMC article.
Recent advances in SCF ubiquitin ligase complex: Clinical implications.
Zheng N, Zhou Q, Wang Z, Wei W. Zheng N, et al. Biochim Biophys Acta. 2016 Aug;1866(1):12-22. doi: 10.1016/j.bbcan.2016.05.001. Epub 2016 May 5. Biochim Biophys Acta. 2016. PMID: 27156687 Free PMC article. Review.
The transcriptional profile of mesenchymal stem cell populations in primary osteoporosis is distinct and shows overexpression of osteogenic inhibitors.
Benisch P, Schilling T, Klein-Hitpass L, Frey SP, Seefried L, Raaijmakers N, Krug M, Regensburger M, Zeck S, Schinke T, Amling M, Ebert R, Jakob F. Benisch P, et al. PLoS One. 2012;7(9):e45142. doi: 10.1371/journal.pone.0045142. Epub 2012 Sep 24. PLoS One. 2012. PMID: 23028809 Free PMC article.
FHY1 mediates nuclear import of the light-activated phytochrome A photoreceptor.
Genoud T, Schweizer F, Tscheuschler A, Debrieux D, Casal JJ, Schäfer E, Hiltbrunner A, Fankhauser C. Genoud T, et al. PLoS Genet. 2008 Aug 1;4(8):e1000143. doi: 10.1371/journal.pgen.1000143. PLoS Genet. 2008. PMID: 18670649 Free PMC article.

References

1. Bai C., Richman, R. and Elledge, S.J. (1994) Human cyclin F. EMBO J., 13, 6087–6098. - PMC - PubMed
1. Bai C., Sen, P., Hofmann, K., Ma, L., Goebl, M., Harper, J.W. and Elledge, S.J. (1996) SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F–box. Cell, 86, 263–274. - PubMed
1. Booher R.N., Alfa, C.E., Hyams, J.S. and Beach, D.H. (1989) The fission yeast cdc2/cdc13/suc1 protein kinase: regulation of catalytic activity and nuclear localization. Cell, 58, 485–497. - PubMed
1. Boulikas T. (1996) Nuclear import of protein kinases and cyclins. J. Cell Biol., 60, 61–82. - PubMed
1. Chen C. and Okayama, H. (1987) High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol., 7, 2745–2752. - PMC - PubMed

Cyclin F regulates the nuclear localization of cyclin B1 through a cyclin-cyclin interaction - PubMed (original) (raw)