Rem2, a new member of the Rem/Rad/Gem/Kir family of Ras-related GTPases - PubMed (original) (raw)
. 2000 Apr 1;347 Pt 1(Pt 1):223-31.
Affiliations
- PMID: 10727423
- PMCID: PMC1220952
Rem2, a new member of the Rem/Rad/Gem/Kir family of Ras-related GTPases
B S Finlin et al. Biochem J. 2000.
Abstract
Here we report the molecular cloning and biochemical characterization of Rem2 (for Rem, Rad and Gem-related 2), a novel GTP-binding protein identified on the basis of its homology with the Rem, Rad, Gem and Kir (RGK) family of Ras-related small GTP-binding proteins. Rem2 mRNA was detected in rat brain and kidney, making it the first member of the RGK family to be expressed at relatively high levels in neuronal tissues. Recombinant Rem2 binds GTP saturably and exhibits a low intrinsic rate of GTP hydrolysis. Surprisingly, the guanine nucleotide dissociation constants for both Rem2 and Rem are significantly different than the majority of the Ras-related GTPases, displaying higher dissociation rates for GTP than GDP. Localization studies with green fluorescent protein (GFP)-tagged recombinant protein fusions indicate that Rem2 has a punctate, plasma membrane localization. Deletion of the C-terminal seven amino acid residues that are conserved in all RGK family members did not affect the cellular distribution of the GFP fusion protein, whereas a larger deletion, including much of the polybasic region of the Rem2 C-terminus, resulted in its redistribution to the cytosol. Thus Rem2 is a GTPase of the RGK family with distinctive biochemical properties and possessing a novel cellular localization signal, consistent with its having a unique role in cell physiology.
Similar articles
- Rem is a new member of the Rad- and Gem/Kir Ras-related GTP-binding protein family repressed by lipopolysaccharide stimulation.
Finlin BS, Andres DA. Finlin BS, et al. J Biol Chem. 1997 Aug 29;272(35):21982-8. doi: 10.1074/jbc.272.35.21982. J Biol Chem. 1997. PMID: 9268335 - Expression of Rem2, an RGK family small GTPase, reduces N-type calcium current without affecting channel surface density.
Chen H, Puhl HL 3rd, Niu SL, Mitchell DC, Ikeda SR. Chen H, et al. J Neurosci. 2005 Oct 19;25(42):9762-72. doi: 10.1523/JNEUROSCI.3111-05.2005. J Neurosci. 2005. PMID: 16237180 Free PMC article. - Roles of 14-3-3 and calmodulin binding in subcellular localization and function of the small G-protein Rem2.
Béguin P, Mahalakshmi RN, Nagashima K, Cher DH, Kuwamura N, Yamada Y, Seino Y, Hunziker W. Béguin P, et al. Biochem J. 2005 Aug 15;390(Pt 1):67-75. doi: 10.1042/BJ20050414. Biochem J. 2005. PMID: 15862114 Free PMC article. - Regulation of voltage-dependent calcium channels by RGK proteins.
Yang T, Colecraft HM. Yang T, et al. Biochim Biophys Acta. 2013 Jul;1828(7):1644-54. doi: 10.1016/j.bbamem.2012.10.005. Epub 2012 Oct 10. Biochim Biophys Acta. 2013. PMID: 23063948 Free PMC article. Review. - Molecular mechanisms and physiological relevance of RGK proteins in the heart.
Meza U, Beqollari D, Bannister RA. Meza U, et al. Acta Physiol (Oxf). 2018 Apr;222(4):e13016. doi: 10.1111/apha.13016. Epub 2017 Dec 29. Acta Physiol (Oxf). 2018. PMID: 29237245 Review.
Cited by
- Rem2 interacts with CaMKII at synapses and restricts long-term potentiation in hippocampus.
Anjum R, Clarke VRJ, Nagasawa Y, Murakoshi H, Paradis S. Anjum R, et al. bioRxiv [Preprint]. 2024 Mar 12:2024.03.11.584540. doi: 10.1101/2024.03.11.584540. bioRxiv. 2024. PMID: 38558974 Free PMC article. Updated. Preprint. - The life cycle of voltage-gated Ca2+ channels in neurons: an update on the trafficking of neuronal calcium channels.
Ferron L, Koshti S, Zamponi GW. Ferron L, et al. Neuronal Signal. 2021 Feb 23;5(1):NS20200095. doi: 10.1042/NS20200095. eCollection 2021 Apr. Neuronal Signal. 2021. PMID: 33664982 Free PMC article. Review. - Designer genetically encoded voltage-dependent calcium channel inhibitors inspired by RGK GTPases.
Colecraft HM. Colecraft HM. J Physiol. 2020 May;598(9):1683-1693. doi: 10.1113/JP276544. Epub 2020 Apr 21. J Physiol. 2020. PMID: 32104913 Free PMC article. Review. - PseudoGTPase domains in p190RhoGAP proteins: a mini-review.
Stiegler AL, Boggon TJ. Stiegler AL, et al. Biochem Soc Trans. 2018 Dec 17;46(6):1713-1720. doi: 10.1042/BST20180481. Epub 2018 Dec 4. Biochem Soc Trans. 2018. PMID: 30514771 Free PMC article. Review. - The Ras-like GTPase Rem2 is a potent inhibitor of calcium/calmodulin-dependent kinase II activity.
Royer L, Herzog JJ, Kenny K, Tzvetkova B, Cochrane JC, Marr MT 2nd, Paradis S. Royer L, et al. J Biol Chem. 2018 Sep 21;293(38):14798-14811. doi: 10.1074/jbc.RA118.003560. Epub 2018 Aug 2. J Biol Chem. 2018. PMID: 30072381 Free PMC article.
References
- Annu Rev Biochem. 1987;56:615-49 - PubMed
- J Biol Chem. 1995 May 26;270(21):12327-30 - PubMed
- Science. 1990 Feb 23;247(4945):939-45 - PubMed
- EMBO J. 1990 Aug;9(8):2351-9 - PubMed
- Cell. 1990 Oct 5;63(1):133-9 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials