Regulation of auxin transport by aminopeptidases and endogenous flavonoids - PubMed (original) (raw)
. 2000 Aug;211(3):315-24.
doi: 10.1007/s004250000300.
Affiliations
- PMID: 10987549
- DOI: 10.1007/s004250000300
Regulation of auxin transport by aminopeptidases and endogenous flavonoids
A Murphy et al. Planta. 2000 Aug.
Abstract
The 1-N-naphthylphthalamic acid (NPA)-binding protein is a putative negative regulator of polar auxin transport that has been shown to block auxin efflux from both whole plant tissues and microsomal membrane vesicles. We previously showed that NPA is hydrolyzed by plasma-membrane amidohydrolases that co-localize with tyrosine, proline, and tryptophan-specific aminopeptidases (APs) in the cotyledonary node, hypocotyl-root transition zone and root distal elongation zone of Arabidopsis thaliana (L.) Heynh. seedlings. Moreover, amino acyl-beta-naphthylamide (aa-NA) conjugates resembling NPA in structure have NPA-like inhibitory activity on growth, suggesting a possible role of APs in NPA action. Here we report that the same aa-NA conjugates and the AP inhibitor bestatin also block auxin efflux from seedling tissue. Bestatin and, to a lesser extent, some aa-NA conjugates were more effective inhibitors of low-affinity specific [3H]NPA-binding than were the flavonoids quercetin and kaempferol but had no effect on high-affinity binding. Since the APs are inhibited by flavonoids, we compared the localization of endogenous flavonoids and APs in seedling tissue. A correlation between AP and flavonoid localization was found in 5- to 6-d-old seedlings. Evidence that these flavonoids regulate auxin accumulation in vivo was obtained using the flavonoid-deficient mutant, tt4. In whole-seedling [14C]indole-3-acetic acid transport studies, the pattern of auxin distribution in the tt4 mutant was shown to be altered. The defect appeared to be in auxin accumulation, as a considerable amount of auxin escaped from the roots. Treatment of the tt4 mutant with the missing intermediate naringenin restored normal auxin distribution and accumulation by the root. These results implicate APs and endogenous flavonoids in the regulation of auxin efflux.
Similar articles
- Arabidopsis AtGSTF2 is regulated by ethylene and auxin, and encodes a glutathione S-transferase that interacts with flavonoids.
Smith AP, Nourizadeh SD, Peer WA, Xu J, Bandyopadhyay A, Murphy AS, Goldsbrough PB. Smith AP, et al. Plant J. 2003 Nov;36(4):433-42. doi: 10.1046/j.1365-313x.2003.01890.x. Plant J. 2003. PMID: 14617075 - PIS1, a negative regulator of the action of auxin transport inhibitors in Arabidopsis thaliana.
Fujita H, Syono K. Fujita H, et al. Plant J. 1997 Sep;12(3):583-95. doi: 10.1046/j.1365-313x.1997.00583.x. Plant J. 1997. PMID: 9351244 - Variation in expression and protein localization of the PIN family of auxin efflux facilitator proteins in flavonoid mutants with altered auxin transport in Arabidopsis thaliana.
Peer WA, Bandyopadhyay A, Blakeslee JJ, Makam SN, Chen RJ, Masson PH, Murphy AS. Peer WA, et al. Plant Cell. 2004 Jul;16(7):1898-911. doi: 10.1105/tpc.021501. Epub 2004 Jun 18. Plant Cell. 2004. PMID: 15208397 Free PMC article. - Phytotropins: receptors and endogenous ligands.
Rubery PH. Rubery PH. Symp Soc Exp Biol. 1990;44:119-46. Symp Soc Exp Biol. 1990. PMID: 2130510 Review. - Naphthylphthalamic acid and the mechanism of polar auxin transport.
Teale W, Palme K. Teale W, et al. J Exp Bot. 2018 Jan 4;69(2):303-312. doi: 10.1093/jxb/erx323. J Exp Bot. 2018. PMID: 28992080 Review.
Cited by
- Transcriptional Regulation and Gene Mapping of Internode Elongation and Late Budding in the Chinese Cabbage Mutant lcc.
Zhang Y, Xuan S, Zhao J, Li H, Lu Y, Li R, Wang Y, Shen S, Sun X, Feng D. Zhang Y, et al. Plants (Basel). 2024 Apr 12;13(8):1083. doi: 10.3390/plants13081083. Plants (Basel). 2024. PMID: 38674492 Free PMC article. - Characterization of leucine aminopeptidase (LAP) activity in sweet pepper fruits during ripening and its inhibition by nitration and reducing events.
Muñoz-Vargas MA, Taboada J, González-Gordo S, Palma JM, Corpas FJ. Muñoz-Vargas MA, et al. Plant Cell Rep. 2024 Mar 11;43(4):92. doi: 10.1007/s00299-024-03179-x. Plant Cell Rep. 2024. PMID: 38466441 Free PMC article. - Flavonoids are involved in salt tolerance through ROS scavenging in the halophyte Atriplex canescens.
Feng S, Yao YT, Wang BB, Li YM, Li L, Bao AK. Feng S, et al. Plant Cell Rep. 2023 Dec 21;43(1):5. doi: 10.1007/s00299-023-03087-6. Plant Cell Rep. 2023. PMID: 38127154 - Evolutionary studies of the bHLH transcription factors belonging to MBW complex: their role in seed development.
Zumajo-Cardona C, Gabrieli F, Anire J, Albertini E, Ezquer I, Colombo L. Zumajo-Cardona C, et al. Ann Bot. 2023 Nov 23;132(3):383-400. doi: 10.1093/aob/mcad097. Ann Bot. 2023. PMID: 37467144 Free PMC article. Review. - PIN structures shed light on their mechanism of auxin efflux.
Joshi C, Napier R. Joshi C, et al. J Exp Bot. 2023 Aug 17;74(15):4377-4383. doi: 10.1093/jxb/erad185. J Exp Bot. 2023. PMID: 37195878 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous