Hypoxia/reoxygenation promotes myocardial angiogenesis via an NF kappa B-dependent mechanism in a rat model of chronic myocardial infarction - PubMed (original) (raw)
Hypoxia/reoxygenation promotes myocardial angiogenesis via an NF kappa B-dependent mechanism in a rat model of chronic myocardial infarction
H Sasaki et al. J Mol Cell Cardiol. 2001 Feb.
Abstract
Therapeutic angiogenesis achieved either through the use of discreet angiogenic proteins or by gene therapy is fast emerging as a highly attractive treatment modality for ischemic heart disease. Herein we examine a novel method of stimulating myocardial angiogenesis by hypoxic preconditioning at both capillary and arteriolar levels, and the potential role of NF kappa B in mediating such a response. We also investigate the functional relevance of such treatment by assessing whether the induced neovascularization can help preserve left ventricular contractile functional reserve in the setting of developing heart failure secondary to myocardial infarction. Male Sprague-Dawley rats were randomly divided into eight groups: normoxia + sham surgery (NS), normoxia + permanent left anterior descending coronary artery (LAD) occlusion (NMI), hypoxic preconditioning + sham surgery (HS), hypoxic preconditioning + permanent LAD occlusion (HMI), PDTC (NF kappa B inhibitor) + hypoxic preconditioning + LAD occlusion (PHMI), PDTC+normoxia + LAD occlusion (PNMI), PDTC + hypoxic preconditioning + sham surgery (PHS) and PDTC + normoxia + sham surgery (PNS). Rats in the preconditioned groups were subjected to systemic hypoxemic hypoxic exposure (10+/-0.4% O2) for 4 h followed by a 24-h period of normoxic reoxygenation prior to undergoing LAD occlusion. Rats in the normoxia groups were time matched with the preconditioned group and maintained under normoxic conditions for the 28-h period prior to LAD occlusion. The HMI group displayed significant increases in capillary as well as arteriolar density after 2, 4 and 7 days post-operation compared to the NMI. Prior PDTC administration prevented such increases in the PHMI group and effectively abolished the pro-angiogenic effect of hypoxic preconditioning (HP). One week after sham surgery or LAD occlusion, rats underwent a pharmacological stress test with dobutamine in progressively increasing doses which revealed significantly elevated values of dp/dt(max) at each dose point in the HMI group compared to the NMI or PHMI groups. Hypoxic preconditioning also decreases endothelial cell injury as determined by the extent of endothelial cell apoptosis using anti-VWF factor labelling and TUNEL assay. The results suggest that HP stimulates myocardial angiogenesis via redox-regulated transcription factor, NF kappa B-dependent pathway to an extent sufficient to exert significant preservation of contractile functional reserve in a rat model of myocardial infarction progressing to heart failure.
Copyright 2001 Academic Press.
Similar articles
- Hypoxic preconditioning triggers myocardial angiogenesis: a novel approach to enhance contractile functional reserve in rat with myocardial infarction.
Sasaki H, Fukuda S, Otani H, Zhu L, Yamaura G, Engelman RM, Das DK, Maulik N. Sasaki H, et al. J Mol Cell Cardiol. 2002 Mar;34(3):335-48. doi: 10.1006/jmcc.2001.1516. J Mol Cell Cardiol. 2002. PMID: 11945025 - Hyperbaric oxygen preconditioning alleviates myocardial ischemic injury in rats.
Han C, Lin L, Zhang W, Zhang L, Lv S, Sun Q, Tao H, Zhang JH, Sun X. Han C, et al. Exp Biol Med (Maywood). 2008 Nov;233(11):1448-53. doi: 10.3181/0801-RM-8. Epub 2008 Aug 14. Exp Biol Med (Maywood). 2008. PMID: 18703748 - Potentiation of angiogenic response by ischemic and hypoxic preconditioning of the heart.
Maulik N, Das DK. Maulik N, et al. J Cell Mol Med. 2002 Jan-Mar;6(1):13-24. doi: 10.1111/j.1582-4934.2002.tb00308.x. J Cell Mol Med. 2002. PMID: 12003666 Free PMC article. Review. - Ischemic preconditioning mediated angiogenic response in the heart.
Maulik N. Maulik N. Antioxid Redox Signal. 2004 Apr;6(2):413-21. doi: 10.1089/152308604322899486. Antioxid Redox Signal. 2004. PMID: 15025943 Review.
Cited by
- NF-kappaB in pancreatic cancer.
Sclabas GM, Fujioka S, Schmidt C, Evans DB, Chiao PJ. Sclabas GM, et al. Int J Gastrointest Cancer. 2003;33(1):15-26. doi: 10.1385/IJGC:33:1:15. Int J Gastrointest Cancer. 2003. PMID: 12909735 Review. - Identifying Candidate Genes for Hypoxia Adaptation of Tibet Chicken Embryos by Selection Signature Analyses and RNA Sequencing.
Liu X, Wang X, Liu J, Wang X, Bao H. Liu X, et al. Genes (Basel). 2020 Jul 20;11(7):823. doi: 10.3390/genes11070823. Genes (Basel). 2020. PMID: 32698384 Free PMC article. - Notch3 deficiency impairs coronary microvascular maturation and reduces cardiac recovery after myocardial ischemia.
Tao YK, Zeng H, Zhang GQ, Chen ST, Xie XJ, He X, Wang S, Wen H, Chen JX. Tao YK, et al. Int J Cardiol. 2017 Jun 1;236:413-422. doi: 10.1016/j.ijcard.2017.01.096. Epub 2017 Jan 24. Int J Cardiol. 2017. PMID: 28131704 Free PMC article. - Regulation of IkappaBalpha function and NF-kappaB signaling: AEBP1 is a novel proinflammatory mediator in macrophages.
Majdalawieh A, Ro HS. Majdalawieh A, et al. Mediators Inflamm. 2010;2010:823821. doi: 10.1155/2010/823821. Epub 2010 Apr 12. Mediators Inflamm. 2010. PMID: 20396415 Free PMC article. Review. - Sildenafil-mediated neovascularization and protection against myocardial ischaemia reperfusion injury in rats: role of VEGF/angiopoietin-1.
Koneru S, Varma Penumathsa S, Thirunavukkarasu M, Vidavalur R, Zhan L, Singal PK, Engelman RM, Das DK, Maulik N. Koneru S, et al. J Cell Mol Med. 2008 Dec;12(6B):2651-64. doi: 10.1111/j.1582-4934.2008.00319.x. Epub 2008 Mar 28. J Cell Mol Med. 2008. PMID: 18373738 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
Miscellaneous