Pyrin N-terminal homology domain- and caspase recruitment domain-dependent oligomerization of ASC - PubMed (original) (raw)

. 2001 Jan 26;280(3):652-5.

doi: 10.1006/bbrc.2000.4190.

Affiliations

• PMID: 11162571
• DOI: 10.1006/bbrc.2000.4190

Pyrin N-terminal homology domain- and caspase recruitment domain-dependent oligomerization of ASC

J Masumoto et al. Biochem Biophys Res Commun. 2001.

Abstract

ASC was first identified as a caspase recruitment domain (CARD)-containing proapoptotic molecule that forms insoluble aggregates during apoptosis. Here, we report both the pyrin N-terminal homology domain (PYD) and CARD domains are involved in the aggregation of ASC. Preliminary experiments indicated that overexpression of ASC formed filament-like aggregates in COS-7 cells. Expression experiments using green fluorescent protein (GFP) constructs showed that not only the GFP-ASC-CARD but also the GFP-ASC-PYD formed filament-like aggregates in COS-7 cells. We confirmed these filament-like aggregates of both the ASC-PYD and the ASC-CARD due to homophilic interaction by immunoprecipitation method. We also demonstrated that the ASC-PYD associated with the ASC-CARD by heterophilic interaction. These observations suggest that the dimerization of the PYD as well as the CARD plays an important role in the oligomerization of ASC as an adaptor molecule.

Copyright 2001 Academic Press.

PubMed Disclaimer

Similar articles

• Expression of apoptosis-associated speck-like protein containing a caspase recruitment domain, a pyrin N-terminal homology domain-containing protein, in normal human tissues.
  Masumoto J, Taniguchi S, Nakayama J, Shiohara M, Hidaka E, Katsuyama T, Murase S, Sagara J. Masumoto J, et al. J Histochem Cytochem. 2001 Oct;49(10):1269-75. doi: 10.1177/002215540104901009. J Histochem Cytochem. 2001. PMID: 11561011
• Role of charged and hydrophobic residues in the oligomerization of the PYRIN domain of ASC.
  Moriya M, Taniguchi S, Wu P, Liepinsh E, Otting G, Sagara J. Moriya M, et al. Biochemistry. 2005 Jan 18;44(2):575-83. doi: 10.1021/bi048374i. Biochemistry. 2005. PMID: 15641782
• Self-oligomerization of ASC PYD domain prevents the assembly of inflammasome in vitro.
  Narayanan KB, Jang TH, Park HH. Narayanan KB, et al. Appl Biochem Biotechnol. 2014 Apr;172(8):3902-12. doi: 10.1007/s12010-014-0819-0. Epub 2014 Mar 2. Appl Biochem Biotechnol. 2014. PMID: 24585381
• Inhibiting the inflammasome: one domain at a time.
  Dorfleutner A, Chu L, Stehlik C. Dorfleutner A, et al. Immunol Rev. 2015 May;265(1):205-16. doi: 10.1111/imr.12290. Immunol Rev. 2015. PMID: 25879295 Free PMC article. Review.
• TMS1/ASC: the cancer connection.
  McConnell BB, Vertino PM. McConnell BB, et al. Apoptosis. 2004 Jan;9(1):5-18. doi: 10.1023/B:APPT.0000012117.32430.0c. Apoptosis. 2004. PMID: 14739594 Review.

Cited by

• ASC filament formation serves as a signal amplification mechanism for inflammasomes.
  Dick MS, Sborgi L, Rühl S, Hiller S, Broz P. Dick MS, et al. Nat Commun. 2016 Jun 22;7:11929. doi: 10.1038/ncomms11929. Nat Commun. 2016. PMID: 27329339 Free PMC article.
• Comprehensive review of ASC structure and function in immune homeostasis and disease.
  Agrawal I, Jha S. Agrawal I, et al. Mol Biol Rep. 2020 Apr;47(4):3077-3096. doi: 10.1007/s11033-020-05345-2. Epub 2020 Mar 2. Mol Biol Rep. 2020. PMID: 32124174 Review.
• Unified polymerization mechanism for the assembly of ASC-dependent inflammasomes.
  Lu A, Magupalli VG, Ruan J, Yin Q, Atianand MK, Vos MR, Schröder GF, Fitzgerald KA, Wu H, Egelman EH. Lu A, et al. Cell. 2014 Mar 13;156(6):1193-1206. doi: 10.1016/j.cell.2014.02.008. Cell. 2014. PMID: 24630722 Free PMC article.
• Activation of inflammasomes and their effects on neuroinflammation at the microelectrode-tissue interface in intracortical implants.
  Franklin ME, Bennett C, Arboite M, Alvarez-Ciara A, Corrales N, Verdelus J, Dietrich WD, Keane RW, de Rivero Vaccari JP, Prasad A. Franklin ME, et al. Biomaterials. 2023 Jun;297:122102. doi: 10.1016/j.biomaterials.2023.122102. Epub 2023 Mar 28. Biomaterials. 2023. PMID: 37015177 Free PMC article.
• Applications of reconstituted inflammasomes in a cell-free system to drug discovery and elucidation of the pathogenesis of autoinflammatory diseases.
  Kaneko N, Iwasaki T, Ito Y, Takeda H, Sawasaki T, Morikawa S, Nakano N, Kurata M, Masumoto J. Kaneko N, et al. Inflamm Regen. 2017 May 3;37:9. doi: 10.1186/s41232-017-0040-y. eCollection 2017. Inflamm Regen. 2017. PMID: 29259708 Free PMC article. Review.

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Miscellaneous

  • NCI CPTAC Assay Portal