Differential effects of UTP, ATP, and adenosine on ciliary activity of human nasal epithelial cells - PubMed (original) (raw)
Comparative Study
. 2001 Jun;280(6):C1485-97.
doi: 10.1152/ajpcell.2001.280.6.C1485.
Affiliations
- PMID: 11350744
- DOI: 10.1152/ajpcell.2001.280.6.C1485
Free article
Comparative Study
Differential effects of UTP, ATP, and adenosine on ciliary activity of human nasal epithelial cells
D M Morse et al. Am J Physiol Cell Physiol. 2001 Jun.
Free article
Abstract
The purinergic regulation of ciliary activity was studied using small, continuously superfused explants of human nasal epithelium. The P2Y(2) purinoceptor (P2Y(2)-R) was identified as the major purinoceptor regulating ciliary beat frequency (CBF); UTP (EC(50) = 4.7 microM), ATP, and adenosine-5'-O-(3-thiotriphosphate) elicited similar maximal responses, approximately twofold over baseline. ATP, however, elicited a post-peak sustained plateau in CBF (1.83 +/- 0.1-fold), whereas the post-peak CBF response to UTP declined over 15 min to a low-level plateau (1.36 +/- 0.16-fold). UDP also stimulated ciliary beating, probably via P2Y(6)-R, with a maximal effect approximately one-half that elicited by P2Y(2)-R stimulation. Not indicated were P2Y(1)-R-, P2Y(4)-R-, or P2Y(11)-R-mediated effects. A(2B)-receptor agonists elicited sustained responses in CBF approximately equal to those from UTP/ATP [5'-(N-ethylcarboxamido)adenosine, EC(50) = 0.09 microM; adenosine, EC(50) = 0.7 microM]. Surprisingly, ADP elicited a sustained stimulation in CBF. The ADP effect and the post-peak sustained portion of the ATP response in CBF were inhibited by the A(2)-R antagonist 8-(p-sulfophenyl)theophylline. Hence, ATP affects ciliary activity through P2Y(2)-R and, after an apparent ectohydrolysis to adenosine, through A(2B)AR.
Similar articles
- P2Y(11) receptor expression by human lymphocytes: evidence for two cAMP-linked purinoceptors.
Conigrave AD, Fernando KC, Gu B, Tasevski V, Zhang W, Luttrell BM, Wiley JS. Conigrave AD, et al. Eur J Pharmacol. 2001 Aug 31;426(3):157-63. doi: 10.1016/s0014-2999(01)01222-5. Eur J Pharmacol. 2001. PMID: 11527539 - Contractile effects of uridine 5'-triphosphate in the rat duodenum.
Johnson CR, Hourani SM. Johnson CR, et al. Br J Pharmacol. 1994 Dec;113(4):1191-6. doi: 10.1111/j.1476-5381.1994.tb17123.x. Br J Pharmacol. 1994. PMID: 7889272 Free PMC article. - P2 purinergic receptors signal to STAT3 in astrocytes: Difference in STAT3 responses to P2Y and P2X receptor activation.
Washburn KB, Neary JT. Washburn KB, et al. Neuroscience. 2006 Oct 13;142(2):411-23. doi: 10.1016/j.neuroscience.2006.06.034. Epub 2006 Aug 14. Neuroscience. 2006. PMID: 16905269 - Purinergic regulation of epithelial transport.
Bucheimer RE, Linden J. Bucheimer RE, et al. J Physiol. 2004 Mar 1;555(Pt 2):311-21. doi: 10.1113/jphysiol.2003.056697. Epub 2003 Dec 23. J Physiol. 2004. PMID: 14694149 Free PMC article. Review. - Coupling of airway ciliary activity and mucin secretion to mechanical stresses by purinergic signaling.
Davis CW, Lazarowski E. Davis CW, et al. Respir Physiol Neurobiol. 2008 Nov 30;163(1-3):208-13. doi: 10.1016/j.resp.2008.05.015. Epub 2008 May 28. Respir Physiol Neurobiol. 2008. PMID: 18635403 Free PMC article. Review.
Cited by
- A succinate/SUCNR1-brush cell defense program in the tracheal epithelium.
Perniss A, Boonen B, Tonack S, Thiel M, Poharkar K, Alnouri MW, Keshavarz M, Papadakis T, Wiegand S, Pfeil U, Richter K, Althaus M, Oberwinkler J, Schütz B, Boehm U, Offermanns S, Leinders-Zufall T, Zufall F, Kummer W. Perniss A, et al. Sci Adv. 2023 Aug 2;9(31):eadg8842. doi: 10.1126/sciadv.adg8842. Epub 2023 Aug 2. Sci Adv. 2023. PMID: 37531421 Free PMC article. - CD73: Friend or Foe in Lung Injury.
Hu XM, Shi NR, Zhang JZ, Zuo YQ, Wang X, Zhao YF, Wu JS. Hu XM, et al. Int J Mol Sci. 2023 Mar 14;24(6):5545. doi: 10.3390/ijms24065545. Int J Mol Sci. 2023. PMID: 36982618 Free PMC article. Review. - Ciliated epithelia are key elements in the recruitment of bacterial partners in the squid-vibrio symbiosis.
Gundlach KA, Nawroth J, Kanso E, Nasrin F, Ruby EG, McFall-Ngai M. Gundlach KA, et al. Front Cell Dev Biol. 2022 Oct 20;10:974213. doi: 10.3389/fcell.2022.974213. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36340026 Free PMC article. - Effect of airway clearance therapies on mucociliary clearance in adults with cystic fibrosis: A randomized controlled trial.
Trimble A, Zeman K, Wu J, Ceppe A, Bennett W, Donaldson S. Trimble A, et al. PLoS One. 2022 May 20;17(5):e0268622. doi: 10.1371/journal.pone.0268622. eCollection 2022. PLoS One. 2022. PMID: 35594286 Free PMC article. Clinical Trial. - Differential Regulation of ATP- and UTP-Evoked Prostaglandin E2 and IL-6 Production from Human Airway Epithelial Cells.
Kountz TS, Jairaman A, Kountz CD, Stauderman KA, Schleimer RP, Prakriya M. Kountz TS, et al. J Immunol. 2021 Sep 1;207(5):1275-1287. doi: 10.4049/jimmunol.2100127. Epub 2021 Aug 13. J Immunol. 2021. PMID: 34389624 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials