Dynamic interactions of p53 with DNA in solution by time-lapse atomic force microscopy - PubMed (original) (raw)
. 2001 Nov 23;314(2):233-43.
doi: 10.1006/jmbi.2001.5129.
Affiliations
- PMID: 11718557
- DOI: 10.1006/jmbi.2001.5129
Dynamic interactions of p53 with DNA in solution by time-lapse atomic force microscopy
Y Jiao et al. J Mol Biol. 2001.
Abstract
Dynamic interactions of the tumor suppressor protein p53 with a DNA fragment containing a p53-specific recognition sequence were directly observed by time-lapse tapping mode atomic force microscopy (AFM) in liquid. The divalent cation Mg(2+) was used to loosely attach both DNA and p53 to a mica surface so they could be imaged by the AFM while interacting with each other. Various interactions of p53 with DNA were observed, including dissociation/re-association, sliding and possibly direct binding to the specific sequence. Two modes of target recognition of p53 were detected: (a) direct binding, and (b) initial non-specific binding with subsequent translocation by one-dimensional diffusion of the protein along the DNA to the specific site.
Copyright 2001 Academic Press.
Similar articles
- Scanning force microscopy of the complexes of p53 core domain with supercoiled DNA.
Jett SD, Cherny DI, Subramaniam V, Jovin TM. Jett SD, et al. J Mol Biol. 2000 Jun 9;299(3):585-92. doi: 10.1006/jmbi.2000.3759. J Mol Biol. 2000. PMID: 10835269 - Conformation and rigidity of DNA microcircles containing waf1 response element for p53 regulatory protein.
Zhou H, Zhang Y, Ou-Yang Z, Lindsay SM, Feng XZ, Balagurumoorthy P, Harrington RE. Zhou H, et al. J Mol Biol. 2001 Feb 16;306(2):227-38. doi: 10.1006/jmbi.2000.4370. J Mol Biol. 2001. PMID: 11237596 - DNA-conformation is an important determinant of sequence-specific DNA binding by tumor suppressor p53.
Kim E, Albrechtsen N, Deppert W. Kim E, et al. Oncogene. 1997 Aug 14;15(7):857-69. doi: 10.1038/sj.onc.1201412. Oncogene. 1997. PMID: 9266973 - The versatile interactions of p53 with DNA: when flexibility serves specificity.
Kim E, Deppert W. Kim E, et al. Cell Death Differ. 2006 Jun;13(6):885-9. doi: 10.1038/sj.cdd.4401909. Cell Death Differ. 2006. PMID: 16543936 Review. No abstract available. - Deposition of supercoiled DNA on mica for scanning force microscopy imaging.
Samorì B, Muzzalupo I, Zuccheri G. Samorì B, et al. Scanning Microsc. 1996;10(4):953-60; discussion 960-2. Scanning Microsc. 1996. PMID: 9890815 Review.
Cited by
- Atomic force microscopy-based approaches for single-molecule investigation of nucleic acid-protein complexes.
Dubrovin EV. Dubrovin EV. Biophys Rev. 2023 Aug 26;15(5):1015-1033. doi: 10.1007/s12551-023-01111-3. eCollection 2023 Oct. Biophys Rev. 2023. PMID: 37974971 Free PMC article. Review. - Atomic force microscopy-A tool for structural and translational DNA research.
Main KHS, Provan JI, Haynes PJ, Wells G, Hartley JA, Pyne ALB. Main KHS, et al. APL Bioeng. 2021 Jul 9;5(3):031504. doi: 10.1063/5.0054294. eCollection 2021 Sep. APL Bioeng. 2021. PMID: 34286171 Free PMC article. Review. - Facilitated Diffusion Mechanisms in DNA Base Excision Repair and Transcriptional Activation.
Esadze A, Stivers JT. Esadze A, et al. Chem Rev. 2018 Dec 12;118(23):11298-11323. doi: 10.1021/acs.chemrev.8b00513. Epub 2018 Oct 31. Chem Rev. 2018. PMID: 30379068 Free PMC article. Review. - Compressive Force Spectroscopy: From Living Cells to Single Proteins.
Wang J, Liu M, Shen Y, Sun J, Shao Z, Czajkowsky DM. Wang J, et al. Int J Mol Sci. 2018 Mar 23;19(4):960. doi: 10.3390/ijms19040960. Int J Mol Sci. 2018. PMID: 29570665 Free PMC article. Review. - Investigating the Influence of Magnesium Ions on p53-DNA Binding Using Atomic Force Microscopy.
Chen Y, Gao T, Wang Y, Yang G. Chen Y, et al. Int J Mol Sci. 2017 Jul 21;18(7):1585. doi: 10.3390/ijms18071585. Int J Mol Sci. 2017. PMID: 28754018 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous