Monoclonal and polyclonal humoral immune response to EC HER-2/NEU peptides with low similarity to the host's proteome - PubMed (original) (raw)

Comparative Study

. 2002 Apr 10;98(5):741-7.

doi: 10.1002/ijc.10259.

Affiliations

• PMID: 11920645
• DOI: 10.1002/ijc.10259

Comparative Study

Monoclonal and polyclonal humoral immune response to EC HER-2/NEU peptides with low similarity to the host's proteome

Abraham Mittelman et al. Int J Cancer. 2002.

Abstract

We are studying peptide immunogenicity as a function of the similarity level to the host's proteome. By using as a model the breast/prostate cancer-associated HER-2/neu antigen, we analyzed the monoclonal and polyclonal humoral immune responses against HER-2/neu peptide motifs not shared with the host proteome. We show here that (i) a mouse monoclonal antibody (MAb) raised against the extracellular domain (EC) of human HER-2/neu oncoprotein recognized a linear peptide motif endowed with low similarity level to the mouse proteome; (ii) likewise, human sera from breast/prostate cancer patients preferentially recognized peptide fragments from the EC of the HER-2/neu oncoprotein having sequences that are not present in the human proteome. Together with previous results obtained in other disease models (cervical cancer-associated HPV16 E7 oncoprotein and Pemphigus vulgaris auto-antigen desmoglein-3), the present data suggest that a low level of sequence similarity to the host's proteome might be an important factor in shaping the pool of B cell epitopes.

Copyright 2002 Wiley-Liss, Inc.

PubMed Disclaimer

Similar articles

• Role of MHC II affinity and molecular mimicry in defining anti-HER-2/neu MAb-3 linear peptide epitope.
  Lucchese A, Stevanovic S, Sinha AA, Mittelman A, Kanduc D. Lucchese A, et al. Peptides. 2003 Feb;24(2):193-7. doi: 10.1016/s0196-9781(03)00026-3. Peptides. 2003. PMID: 12668202
• Inhibition of tumor cell growth by antibodies induced after vaccination with peptides derived from the extracellular domain of Her-2/neu.
  Jasinska J, Wagner S, Radauer C, Sedivy R, Brodowicz T, Wiltschke C, Breiteneder H, Pehamberger H, Scheiner O, Wiedermann U, Zielinski CC. Jasinska J, et al. Int J Cancer. 2003 Dec 20;107(6):976-83. doi: 10.1002/ijc.11485. Int J Cancer. 2003. PMID: 14601058
• Individuation of monoclonal anti-HPV16 E7 antibody linear peptide epitope by computational biology.
  Kanduc D, Lucchese A, Mittelman A. Kanduc D, et al. Peptides. 2001 Dec;22(12):1981-5. doi: 10.1016/s0196-9781(01)00539-3. Peptides. 2001. PMID: 11786180
• Cancer vaccines targeting the HER2/neu oncogenic protein.
  Disis ML, Schiffman K. Disis ML, et al. Semin Oncol. 2001 Dec;28(6 Suppl 18):12-20. Semin Oncol. 2001. PMID: 11774201 Review.
• Epitopic peptides with low similarity to the host proteome: towards biological therapies without side effects.
  Kanduc D. Kanduc D. Expert Opin Biol Ther. 2009 Jan;9(1):45-53. doi: 10.1517/14712590802614041. Expert Opin Biol Ther. 2009. PMID: 19063692 Review.

Cited by

• Preliminary data on Pemphigus vulgaris treatment by a proteomics-defined peptide: a case report.
  Angelini G, Bonamonte D, Lucchese A, Favia G, Serpico R, Mittelman A, Simone S, Sinha AA, Kanduc D. Angelini G, et al. J Transl Med. 2006 Oct 24;4:43. doi: 10.1186/1479-5876-4-43. J Transl Med. 2006. PMID: 17062151 Free PMC article.
• Beyond the Gut: A Systematic Review of Oral Manifestations in Celiac Disease.
  Lucchese A, Di Stasio D, De Stefano S, Nardone M, Carinci F. Lucchese A, et al. J Clin Med. 2023 Jun 6;12(12):3874. doi: 10.3390/jcm12123874. J Clin Med. 2023. PMID: 37373569 Free PMC article. Review.
• Proposing low-similarity peptide vaccines against Mycobacterium tuberculosis.
  Lucchese G, Stufano A, Kanduc D. Lucchese G, et al. J Biomed Biotechnol. 2010;2010:832341. doi: 10.1155/2010/832341. Epub 2010 Jun 3. J Biomed Biotechnol. 2010. PMID: 20625421 Free PMC article.
• The tumor microenvironment and its contribution to tumor evolution toward metastasis.
  Lorusso G, Rüegg C. Lorusso G, et al. Histochem Cell Biol. 2008 Dec;130(6):1091-103. doi: 10.1007/s00418-008-0530-8. Epub 2008 Nov 6. Histochem Cell Biol. 2008. PMID: 18987874 Review.
• Epitope definition by proteomic similarity analysis: identification of the linear determinant of the anti-Dsg3 MAb 5H10.
  Lucchese A, Mittelman A, Lin MS, Kanduc D, Sinha AA. Lucchese A, et al. J Transl Med. 2004 Dec 11;2(1):43. doi: 10.1186/1479-5876-2-43. J Transl Med. 2004. PMID: 15588331 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal
  • SciCrunch