Redox factor-1: an extra-nuclear role in the regulation of endothelial oxidative stress and apoptosis - PubMed (original) (raw)
Redox factor-1: an extra-nuclear role in the regulation of endothelial oxidative stress and apoptosis
P Angkeow et al. Cell Death Differ. 2002 Jul.
Abstract
The rac1 GTPase promotes oxidative stress through reactive oxygen species (ROS) production, whereas the DNA repair enzyme and transcriptional regulator redox factor-1 (ref-1) protects against cell death due to oxidative stimuli. However, the function of ref-1 in regulating intracellular oxidative stress, particularly that induced by rac1, has not been defined. We examined the role of ref-1 in vascular endothelial cell oxidative stress and apoptosis. Ref-1 was expressed in both the cytoplasm and nuclei of resting endothelial cells. Cytoplasmic ref-1 translocated to the nucleus with the oxidative trigger hypoxia/reoxygenation (H/R). Forced cytoplasmic overexpression of ref-1 suppressed H/R-induced oxidative stress (H(2)O(2) production), NF-kappaB activation, and apoptosis, and also mitigated rac1-regulated H(2)O(2) production and NF-kappaB transcriptional activity. We conclude that inhibition of oxidative stress is another mechanism by which ref-1 protects against apoptosis, and that this is achieved through modulation of cytoplasmic rac1-regulated ROS generation. This suggests a novel extra-nuclear function of ref-1.
Similar articles
- Redox factor-1/APE suppresses oxidative stress by inhibiting the rac1 GTPase.
Ozaki M, Suzuki S, Irani K. Ozaki M, et al. FASEB J. 2002 Jun;16(8):889-90. doi: 10.1096/fj.01-0664fje. Epub 2002 Apr 23. FASEB J. 2002. PMID: 12039869 - Loss of redox factor 1 decreases NF-kappaB activity and increases susceptibility of endothelial cells to apoptosis.
Guan Z, Basi D, Li Q, Mariash A, Xia YF, Geng JG, Kao E, Hall JL. Guan Z, et al. Arterioscler Thromb Vasc Biol. 2005 Jan;25(1):96-101. doi: 10.1161/01.ATV.0000150418.14698.75. Epub 2004 Nov 11. Arterioscler Thromb Vasc Biol. 2005. PMID: 15539619 - Free radicals, metals and antioxidants in oxidative stress-induced cancer.
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Valko M, et al. Chem Biol Interact. 2006 Mar 10;160(1):1-40. doi: 10.1016/j.cbi.2005.12.009. Epub 2006 Jan 23. Chem Biol Interact. 2006. PMID: 16430879 Review. - The structure and functions of the HAP1/Ref-1 protein.
Rothwell DG, Barzilay G, Gorman M, Morera S, Freemont P, Hickson ID. Rothwell DG, et al. Oncol Res. 1997;9(6-7):275-80. Oncol Res. 1997. PMID: 9406232 Review.
Cited by
- Examining the function of macrophage oxidative stress response and immune system in glioblastoma multiforme through analysis of single-cell transcriptomics.
Xing J, Cai H, Lin Z, Zhao L, Xu H, Song Y, Wang Z, Liu C, Hu G, Zheng J, Ren L, Wei Z. Xing J, et al. Front Immunol. 2024 Jan 11;14:1288137. doi: 10.3389/fimmu.2023.1288137. eCollection 2023. Front Immunol. 2024. PMID: 38274828 Free PMC article. - Astrocyte DNA damage and response upon acute exposure to ethanol and corticosterone.
Reyes-Ábalos AL, Álvarez-Zabaleta M, Olivera-Bravo S, Di Tomaso MV. Reyes-Ábalos AL, et al. Front Toxicol. 2024 Jan 8;5:1277047. doi: 10.3389/ftox.2023.1277047. eCollection 2023. Front Toxicol. 2024. PMID: 38259729 Free PMC article. - Targeting the Main Sources of Reactive Oxygen Species Production: Possible Therapeutic Implications in Chronic Pain.
Cheng PF, Yuan-He, Ge MM, Ye DW, Chen JP, Wang JX. Cheng PF, et al. Curr Neuropharmacol. 2024;22(12):1960-1985. doi: 10.2174/1570159X22999231024140544. Curr Neuropharmacol. 2024. PMID: 37921169 Free PMC article. Review. - More than Just Antioxidants: Redox-Active Components and Mechanisms Shaping Redox Signalling Network.
Kuczyńska M, Jakubek P, Bartoszek A. Kuczyńska M, et al. Antioxidants (Basel). 2022 Dec 4;11(12):2403. doi: 10.3390/antiox11122403. Antioxidants (Basel). 2022. PMID: 36552611 Free PMC article. Review. - Research progress on oxidative stress regulating different types of neuronal death caused by epileptic seizures.
Sun H, Li X, Guo Q, Liu S. Sun H, et al. Neurol Sci. 2022 Nov;43(11):6279-6298. doi: 10.1007/s10072-022-06302-6. Epub 2022 Aug 4. Neurol Sci. 2022. PMID: 35927358 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous