GLUT2 is a high affinity glucosamine transporter - PubMed (original) (raw)
GLUT2 is a high affinity glucosamine transporter
Marc Uldry et al. FEBS Lett. 2002.
Free article
Abstract
When expressed in Xenopus oocytes, GLUT1, 2 and 4 transport glucosamine with V(max) values that are three- to four-fold lower than for glucose. The K(m)s for glucosamine and glucose of GLUT1 and GLUT4 were similar. In contrast, GLUT2 had a much higher apparent affinity for glucosamine than for glucose (K(m)=0.8+/-0.1 mM vs. approximately 17-20 mM). Glucosamine transport by GLUT2 was confirmed in mammalian cells and, using hepatocytes from control or GLUT2-null mice, HgCl(2)-inhibitable glucosamine uptake by liver was shown to be exclusively through GLUT2. These data have implications for glucosamine effects on impaired glucose metabolism and for structure-function studies of transporter sugar binding sites.
Similar articles
- Kinetic analysis of the liver-type (GLUT2) and brain-type (GLUT3) glucose transporters in Xenopus oocytes: substrate specificities and effects of transport inhibitors.
Colville CA, Seatter MJ, Jess TJ, Gould GW, Thomas HM. Colville CA, et al. Biochem J. 1993 Mar 15;290 ( Pt 3)(Pt 3):701-6. doi: 10.1042/bj2900701. Biochem J. 1993. PMID: 8457197 Free PMC article. - The glucose transporter-2 (GLUT2) is a low affinity dehydroascorbic acid transporter.
Mardones L, Ormazabal V, Romo X, Jaña C, Binder P, Peña E, Vergara M, Zúñiga FA. Mardones L, et al. Biochem Biophys Res Commun. 2011 Jun 24;410(1):7-12. doi: 10.1016/j.bbrc.2011.05.070. Epub 2011 May 20. Biochem Biophys Res Commun. 2011. PMID: 21621509 - Mammalian facilitative glucose transporters: evidence for similar substrate recognition sites in functionally monomeric proteins.
Burant CF, Bell GI. Burant CF, et al. Biochemistry. 1992 Oct 27;31(42):10414-20. doi: 10.1021/bi00157a032. Biochemistry. 1992. PMID: 1420159 - Structure, function, and regulation of the mammalian facilitative glucose transporter gene family.
Olson AL, Pessin JE. Olson AL, et al. Annu Rev Nutr. 1996;16:235-56. doi: 10.1146/annurev.nu.16.070196.001315. Annu Rev Nutr. 1996. PMID: 8839927 Review. - cypate-d: -(+)-glucosamine (cyp-GlcN), and d: -(+)-glucosamine-cypate-d: -(+)-glucosamine (cyp-2GlcN).
Chopra A. Chopra A. 2012 Aug 8 [updated 2012 Sep 13]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004–2013. 2012 Aug 8 [updated 2012 Sep 13]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004–2013. PMID: 22993875 Free Books & Documents. Review.
Cited by
- Computational modeling of glucose transport in pancreatic β-cells identifies metabolic thresholds and therapeutic targets in diabetes.
Luni C, Marth JD, Doyle FJ 3rd. Luni C, et al. PLoS One. 2012;7(12):e53130. doi: 10.1371/journal.pone.0053130. Epub 2012 Dec 27. PLoS One. 2012. PMID: 23300881 Free PMC article. - Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs.
Zhao W, Liu F, Marth CD, Green MP, Le HH, Leury BJ, Bell AW, Dunshea FR, Cottrell JJ. Zhao W, et al. Int J Mol Sci. 2021 Apr 16;22(8):4147. doi: 10.3390/ijms22084147. Int J Mol Sci. 2021. PMID: 33923747 Free PMC article. - Role of UDP-N-acetylglucosamine (GlcNAc) and O-GlcNAcylation of hyaluronan synthase 2 in the control of chondroitin sulfate and hyaluronan synthesis.
Vigetti D, Deleonibus S, Moretto P, Karousou E, Viola M, Bartolini B, Hascall VC, Tammi M, De Luca G, Passi A. Vigetti D, et al. J Biol Chem. 2012 Oct 12;287(42):35544-35555. doi: 10.1074/jbc.M112.402347. Epub 2012 Aug 10. J Biol Chem. 2012. PMID: 22887999 Free PMC article. - Water-Soluble Chitosan Conjugated DOTA-Bombesin Peptide Capped Gold Nanoparticles as a Targeted Therapeutic Agent for Prostate Cancer.
Tangthong T, Piroonpan T, Thipe VC, Khoobchandani M, Katti K, Katti KV, Pasanphan W. Tangthong T, et al. Nanotechnol Sci Appl. 2021 Mar 18;14:69-89. doi: 10.2147/NSA.S301942. eCollection 2021. Nanotechnol Sci Appl. 2021. PMID: 33776426 Free PMC article. - Ontogeny of D-mannose transport and metabolism in rat small intestine.
Cano M, Ilundain AA. Cano M, et al. J Membr Biol. 2010 Jun;235(2):101-8. doi: 10.1007/s00232-010-9259-0. Epub 2010 Jun 4. J Membr Biol. 2010. PMID: 20523973
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous