A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta - PubMed (original) (raw)
. 2002 Oct 4;277(40):36955-61.
doi: 10.1074/jbc.M206210200. Epub 2002 Jul 29.
Affiliations
- PMID: 12147701
- DOI: 10.1074/jbc.M206210200
Free article
A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta
Chie Tanji et al. J Biol Chem. 2002.
Free article
Abstract
Glycogen synthase kinase-3 (GSK-3) is regulated by various extracellular ligands and phosphorylates many substrates, thereby regulating cellular functions. Using yeast two-hybrid screening, we found that GSK-3beta binds to AKAP220, which is known to act as an A-kinase anchoring protein. GSK-3beta formed a complex with AKAP220 in intact cells at the endogenous level. Cyclic AMP-dependent protein kinase (PKA) and type 1 protein phosphatase (PP1) were also detected in this complex, suggesting that AKAP220, GSK-3beta, PKA, and PP1 form a quaternary complex. It has been reported that PKA phosphorylates GSK-3beta, thereby decreasing its activity. When COS cells were treated with dibutyryl cyclic AMP to activate PKA, the activity of GSK-3beta bound to AKAP220 decreased more markedly than the total GSK-3beta activity. Calyculin A, a protein phosphatase inhibitor, also inhibited the activity of GSK-3beta bound to AKAP220 more strongly than the total GSK-3beta activity. These results suggest that PKA and PP1 regulate the activity of GSK-3beta efficiently by forming a complex with AKAP220.
Similar articles
- Protein Kinase A Opposes the Phosphorylation-dependent Recruitment of Glycogen Synthase Kinase 3β to A-kinase Anchoring Protein 220.
Whiting JL, Nygren PJ, Tunquist BJ, Langeberg LK, Seternes OM, Scott JD. Whiting JL, et al. J Biol Chem. 2015 Aug 7;290(32):19445-57. doi: 10.1074/jbc.M115.654822. Epub 2015 Jun 18. J Biol Chem. 2015. PMID: 26088133 Free PMC article. - Phosphorylation of TIMAP by glycogen synthase kinase-3beta activates its associated protein phosphatase 1.
Li L, Kozlowski K, Wegner B, Rashid T, Yeung T, Holmes C, Ballermann BJ. Li L, et al. J Biol Chem. 2007 Aug 31;282(35):25960-9. doi: 10.1074/jbc.M703532200. Epub 2007 Jul 3. J Biol Chem. 2007. PMID: 17609201 - Regulation of type 1 protein phosphatase/inhibitor-2 complex by glycogen synthase kinase-3beta in intact cells.
Sakashita G, Shima H, Komatsu M, Urano T, Kikuchi A, Kikuchi K. Sakashita G, et al. J Biochem. 2003 Feb;133(2):165-71. doi: 10.1093/jb/mvg020. J Biochem. 2003. PMID: 12761178 - GSK-3β activity and hyperdopamine-dependent behaviors.
Li YC, Gao WJ. Li YC, et al. Neurosci Biobehav Rev. 2011 Jan;35(3):645-54. doi: 10.1016/j.neubiorev.2010.08.001. Epub 2010 Aug 18. Neurosci Biobehav Rev. 2011. PMID: 20727368 Free PMC article. Review. - Many faces and functions of GSKIP: a temporospatial regulation view.
Wu NS, Lin YF, Ma IC, Ko HJ, Hong YR. Wu NS, et al. Cell Signal. 2022 Sep;97:110391. doi: 10.1016/j.cellsig.2022.110391. Epub 2022 Jun 18. Cell Signal. 2022. PMID: 35728705 Review.
Cited by
- When You Come to a Fork in the Road, Take It: Wnt Signaling Activates Multiple Pathways through the APC/Axin/GSK-3 Complex.
Li C, Furth EE, Rustgi AK, Klein PS. Li C, et al. Cells. 2023 Sep 12;12(18):2256. doi: 10.3390/cells12182256. Cells. 2023. PMID: 37759479 Free PMC article. Review. - AKAP220 protein organizes signaling elements that impact cell migration.
Logue JS, Whiting JL, Tunquist B, Sacks DB, Langeberg LK, Wordeman L, Scott JD. Logue JS, et al. J Biol Chem. 2011 Nov 11;286(45):39269-81. doi: 10.1074/jbc.M111.277756. Epub 2011 Sep 2. J Biol Chem. 2011. PMID: 21890631 Free PMC article. - A-kinase anchoring protein mediates TRPV1 thermal hyperalgesia through PKA phosphorylation of TRPV1.
Jeske NA, Diogenes A, Ruparel NB, Fehrenbacher JC, Henry M, Akopian AN, Hargreaves KM. Jeske NA, et al. Pain. 2008 Sep 15;138(3):604-616. doi: 10.1016/j.pain.2008.02.022. Epub 2008 Apr 1. Pain. 2008. PMID: 18381233 Free PMC article. - Role of GSK3 Signaling in Neuronal Morphogenesis.
Kim YT, Hur EM, Snider WD, Zhou FQ. Kim YT, et al. Front Mol Neurosci. 2011 Nov 23;4:48. doi: 10.3389/fnmol.2011.00048. eCollection 2011. Front Mol Neurosci. 2011. PMID: 22131966 Free PMC article. - H-prune-nm23-H1 protein complex and correlation to pathways in cancer metastasis.
Garzia L, Roma C, Tata N, Pagnozzi D, Pucci P, Zollo M. Garzia L, et al. J Bioenerg Biomembr. 2006 Aug;38(3-4):205-13. doi: 10.1007/s10863-006-9036-z. J Bioenerg Biomembr. 2006. PMID: 17103319
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous