Role of ANC-1 in tethering nuclei to the actin cytoskeleton - PubMed (original) (raw)
. 2002 Oct 11;298(5592):406-9.
doi: 10.1126/science.1075119. Epub 2002 Aug 8.
Affiliations
- PMID: 12169658
- DOI: 10.1126/science.1075119
Role of ANC-1 in tethering nuclei to the actin cytoskeleton
Daniel A Starr et al. Science. 2002.
Abstract
Mutations in anc-1 (nuclear anchorage defective) disrupt the positioning of nuclei and mitochondria in Caenorhabditis elegans. ANC-1 is shown to consist of mostly coiled regions with a nuclear envelope localization domain (called the KASH domain) and an actin-binding domain; this structure was conserved with the Drosophila protein Msp-300 and the mammalian Syne proteins. Antibodies against ANC-1 localized cytoplasmically and were enriched at the nuclear periphery in an UNC-84-dependent manner. Overexpression of the KASH domain or the actin-binding domain caused a dominant negative anchorage defect. Thus, ANC-1 may connect nuclei to the cytoskeleton by interacting with UNC-84 at the nuclear envelope and with actin in the cytoplasm.
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