Activation of the specific neurotrophin receptors TrkA, TrkB and TrkC influences the function of eosinophils - PubMed (original) (raw)
Activation of the specific neurotrophin receptors TrkA, TrkB and TrkC influences the function of eosinophils
O Noga et al. Clin Exp Allergy. 2002 Sep.
Abstract
Background: Recent studies have shown that nerve growth factor (NGF) can act on several immune cells as well as residential cells. But little is known about their role in modulating eosinophil function via activation of high-affinity receptors.
Objectives: The aim of this study was to assess whether eosinophils express functional receptors and if their function is influenced by NGF, brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3).
Methods: Eosinophils were purified by negative immunoselection (purity > 96%). High-affinity neurotrophin receptors were demonstrated by reverse transcription polymerase chain reaction, western blotting and flow-cytometry analysis. Functionality of receptors was demonstrated by receptor phosphorylation after ligand binding. Eosinophils were incubated with NGF, BDNF and NT-3, and cells and supernatants were collected for measurement of the mediators IL-4, IL-5, IL-8, transforming growth factor (TGF)-beta1, eosinophil cationic protein (ECP), eosinophil protein X (EPX) as well as eosinophil viability.
Results: Eosinophils expressed mRNA for neurotrophin receptors. Proteins were detectable by western blot and fluorescent-activated cell sorter analysis. The receptors were phosphorylated after stimulation with neurotrophins. After NGF stimulation, a significant increase in IL-4 was detectable. BDNF and NT-3 stimulation led to a significant increase in EPX. Eosinophil viability was not influenced.
Conclusions: Eosinophils express the functionally active receptors TrkA, TrkB and TrkC. Receptor activation stimulates eosinophils. This might be an additional pathway regulating inflammatory responses in allergic reactions.
Comment in
- The role of neurotrophins in the pathogenesis of asthma and related diseases: allergy and asthma as prototypic neuro-immune diseases?
Nockher WA, Renz H. Nockher WA, et al. Clin Exp Allergy. 2002 Sep;32(9):1266-8. doi: 10.1046/j.1365-2745.2002.01489.x. Clin Exp Allergy. 2002. PMID: 12220461 No abstract available.
Similar articles
- The neurotrophins nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 are survival and activation factors for eosinophils in patients with allergic bronchial asthma.
Nassenstein C, Braun A, Erpenbeck VJ, Lommatzsch M, Schmidt S, Krug N, Luttmann W, Renz H, Virchow JC Jr. Nassenstein C, et al. J Exp Med. 2003 Aug 4;198(3):455-67. doi: 10.1084/jem.20010897. J Exp Med. 2003. PMID: 12900521 Free PMC article. - The production, storage and release of the neurotrophins nerve growth factor, brain-derived neurotrophic factor and neurotrophin-3 by human peripheral eosinophils in allergics and non-allergics.
Noga O, Englmann C, Hanf G, Grützkau A, Seybold J, Kunkel G. Noga O, et al. Clin Exp Allergy. 2003 May;33(5):649-54. doi: 10.1046/j.1365-2222.2003.01586.x. Clin Exp Allergy. 2003. PMID: 12752594 - The role of neurotrophins in the pathophysiology of allergic rhinitis.
Raap U, Braunstahl GJ. Raap U, et al. Curr Opin Allergy Clin Immunol. 2010 Feb;10(1):8-13. doi: 10.1097/ACI.0b013e328334f5de. Curr Opin Allergy Clin Immunol. 2010. PMID: 19935061 Review. - Molecular interactions between neurotrophin receptors.
Dechant G. Dechant G. Cell Tissue Res. 2001 Aug;305(2):229-38. doi: 10.1007/s004410100378. Cell Tissue Res. 2001. PMID: 11545260 Review.
Cited by
- TRPV1 Channel in Human Eosinophils: Functional Expression and Inflammatory Modulation.
Weihrauch T, Gray N, Wiebe D, Schmelz M, Limberg MM, Raap U. Weihrauch T, et al. Int J Mol Sci. 2024 Feb 5;25(3):1922. doi: 10.3390/ijms25031922. Int J Mol Sci. 2024. PMID: 38339203 Free PMC article. - Neurotrophins: Expression of Brain-Lung Axis Development.
Manti S, Xerra F, Spoto G, Butera A, Gitto E, Di Rosa G, Nicotera AG. Manti S, et al. Int J Mol Sci. 2023 Apr 11;24(8):7089. doi: 10.3390/ijms24087089. Int J Mol Sci. 2023. PMID: 37108250 Free PMC article. Review. - Neurotrophins: Neuroimmune Interactions in Human Atopic Diseases.
Weihrauch T, Limberg MM, Gray N, Schmelz M, Raap U. Weihrauch T, et al. Int J Mol Sci. 2023 Mar 24;24(7):6105. doi: 10.3390/ijms24076105. Int J Mol Sci. 2023. PMID: 37047077 Free PMC article. Review. - Further Understanding of Neuro-Immune Interactions in Allergy: Implications in Pathophysiology and Role in Disease Progression.
Konstantinou GN, Konstantinou GN, Koulias C, Petalas K, Makris M. Konstantinou GN, et al. J Asthma Allergy. 2022 Sep 10;15:1273-1291. doi: 10.2147/JAA.S282039. eCollection 2022. J Asthma Allergy. 2022. PMID: 36117919 Free PMC article. Review. - Eosinophils and eosinophil-associated disorders: immunological, clinical, and molecular complexity.
Valent P, Degenfeld-Schonburg L, Sadovnik I, Horny HP, Arock M, Simon HU, Reiter A, Bochner BS. Valent P, et al. Semin Immunopathol. 2021 Jun;43(3):423-438. doi: 10.1007/s00281-021-00863-y. Epub 2021 May 30. Semin Immunopathol. 2021. PMID: 34052871 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous