Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA - PubMed (original) (raw)
Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA
Andrei Alexandrov et al. RNA. 2002 Oct.
Abstract
7-methylguanosine (m7G) modification of tRNA occurs widely in eukaryotes and bacteria, is nearly always found at position 46, and is one of the few modifications that confers a positive charge to the base. Screening of a Saccharomyces cerevisiae genomic library of purified GST-ORF fusion proteins reveals two previously uncharacterized proteins that copurify with m7G methyltransferase activity on pre-tRNA(Phe). ORF YDL201w encodes Trm8, a protein that is highly conserved in prokaryotes and eukaryotes and that contains an S-adenosylmethionine binding domain. ORF YDR165w encodes Trm82, a less highly conserved protein containing putative WD40 repeats, which are often implicated in macromolecular interactions. Neither protein has significant sequence similarity to yeast Abd1, which catalyzes m7G modification of the 5' cap of mRNA, other than the methyltransferase motif shared by Trm8 and Abd1. Several lines of evidence indicate that both Trm8 and Trm82 proteins are required for tRNA m7G-methyltransferase activity: Extracts derived from strains lacking either gene have undetectable m7G methyltransferase activity, RNA from strains lacking either gene have much reduced m7G, and coexpression of both proteins is required to overproduce activity. Aniline cleavage mapping shows that Trm8/Trm82 proteins modify pre-tRNAPhe at G46, the site that is modified in vivo. Trm8 and Trm82 proteins form a complex, as affinity purification of Trm8 protein causes copurification of Trm82 protein in approximate equimolar yield. This functional two-protein family appears to be retained in eukaryotes, as expression of both corresponding human proteins, METTL1 and WDR4, is required for m7G-methyltransferase activity.
Similar articles
- tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p.
Alexandrov A, Grayhack EJ, Phizicky EM. Alexandrov A, et al. RNA. 2005 May;11(5):821-30. doi: 10.1261/rna.2030705. Epub 2005 Apr 5. RNA. 2005. PMID: 15811913 Free PMC article. - Production of yeast tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex) in a wheat germ cell-free translation system.
Matsumoto K, Tomikawa C, Toyooka T, Ochi A, Takano Y, Takayanagi N, Abe M, Endo Y, Hori H. Matsumoto K, et al. J Biotechnol. 2008 Feb 29;133(4):453-60. doi: 10.1016/j.jbiotec.2007.11.009. Epub 2007 Nov 24. J Biotechnol. 2008. PMID: 18164779 - Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system.
Muneyoshi Y, Matsumoto K, Tomikawa C, Toyooka T, Ochi A, Masaoka T, Endo Y, Hori H. Muneyoshi Y, et al. Nucleic Acids Symp Ser (Oxf). 2007;(51):359-60. doi: 10.1093/nass/nrm180. Nucleic Acids Symp Ser (Oxf). 2007. PMID: 18029735 - Two-subunit enzymes involved in eukaryotic post-transcriptional tRNA modification.
Guy MP, Phizicky EM. Guy MP, et al. RNA Biol. 2014;11(12):1608-18. doi: 10.1080/15476286.2015.1008360. RNA Biol. 2014. PMID: 25625329 Free PMC article. Review. - 7-Methylguanosine Modifications in Transfer RNA (tRNA).
Tomikawa C. Tomikawa C. Int J Mol Sci. 2018 Dec 17;19(12):4080. doi: 10.3390/ijms19124080. Int J Mol Sci. 2018. PMID: 30562954 Free PMC article. Review.
Cited by
- Decoding the epitranscriptome: a new frontier for cancer therapy and drug resistance.
Tang L, Tian H, Min Q, You H, Yin M, Yang L, Zhao Y, Wu X, Li M, Du F, Chen Y, Deng S, Li X, Chen M, Gu L, Sun Y, Xiao Z, Li W, Shen J. Tang L, et al. Cell Commun Signal. 2024 Oct 21;22(1):513. doi: 10.1186/s12964-024-01854-w. Cell Commun Signal. 2024. PMID: 39434167 Review. - Roles of clinical application of lenvatinib and its resistance mechanism in advanced hepatocellular carcinoma (Review).
Ye G, Ye M, Jin X. Ye G, et al. Am J Cancer Res. 2024 Sep 15;14(9):4113-4171. doi: 10.62347/UJVP4361. eCollection 2024. Am J Cancer Res. 2024. PMID: 39417171 Free PMC article. Review. - Mettl1-dependent m7G tRNA modification is essential for maintaining spermatogenesis and fertility in Drosophila melanogaster.
Kaneko S, Miyoshi K, Tomuro K, Terauchi M, Tanaka R, Kondo S, Tani N, Ishiguro KI, Toyoda A, Kamikouchi A, Noguchi H, Iwasaki S, Saito K. Kaneko S, et al. Nat Commun. 2024 Sep 24;15(1):8147. doi: 10.1038/s41467-024-52389-0. Nat Commun. 2024. PMID: 39317727 Free PMC article. - Metabolic rewiring during bone development underlies tRNA m7G-associated primordial dwarfism.
Li Q, Jiang S, Lei K, Han H, Chen Y, Lin W, Xiong Q, Qi X, Gan X, Sheng R, Wang Y, Zhang Y, Ma J, Li T, Lin S, Zhou C, Chen D, Yuan Q. Li Q, et al. J Clin Invest. 2024 Sep 10;134(20):e177220. doi: 10.1172/JCI177220. J Clin Invest. 2024. PMID: 39255038 Free PMC article. - Cilia defects upon loss of WDR4 are linked to proteasomal hyperactivity and ubiquitin shortage.
Burkhalter MD, Stiff T, Maerz LD, Casar Tena T, Wiese H, Gerhards J, Sailer SA, Vu LAT, Duong Phu M, Donow C, Alupei M, Iben S, Groth M, Wiese S, Church JA, Jeggo PA, Philipp M. Burkhalter MD, et al. Cell Death Dis. 2024 Sep 9;15(9):660. doi: 10.1038/s41419-024-07042-5. Cell Death Dis. 2024. PMID: 39251572 Free PMC article.
References
- Nucleic Acids Res. 1976 Nov;3(11):3109-22 - PubMed
- Nucleic Acids Res. 1988 Nov 25;16(22):10881-90 - PubMed
- Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760-4 - PubMed
- J Biol Chem. 1982 Aug 25;257(16):9759-69 - PubMed
- Gene. 1988 Dec 30;74(2):527-34 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous