Isolation and characterization of runxa and runxb, zebrafish members of the runt family of transcriptional regulators - PubMed (original) (raw)
Isolation and characterization of runxa and runxb, zebrafish members of the runt family of transcriptional regulators
Caroline Erter Burns et al. Exp Hematol. 2002 Dec.
Free article
Abstract
Objective: The AML/RUNX family of transcription factors plays important roles in hematopoiesis, neurogenesis, bone development, and segmentation in vertebrate embryos. The aim of this study was to isolate runt-related genes in a genetically and embryologically exploitable system, the zebrafish, and characterize their function during hematopoietic development.
Materials and methods: Two runt-related genes were isolated by degenerate PCR and standard library screening, and a radiation hybrid panel, T51 RH, was used to resolve their chromosomal localization. In situ hybridization demonstrated their expression whereas their transcriptional activity was assessed using an AML1-responsive reporter gene in the MLA 144 T-cell line.
Results: We isolated the zebrafish runxa and runxb cDNAs, which encode proteins highly homologous to the human and murine Runx1 (AML1) and Runx3 (AML2) proteins. In contrast to a recent report, we detected runxa expression in both hematopoietic and neural tissues of the developing zebrafish. runxa transcripts first appear during segmentation in bilateral mesodermal cells that coexpress one of the earliest blood and endothelial cell markers, scl/tal-1. By 24 hours postfertilization (hpf), runxa transcripts are seen in the ventral wall of the dorsal aorta. Hematopoietic runxa expression is lost in cloche mutants, which are defective in blood and endothelial cell formation. runxb transcripts are seen in nonhematopoietic domains. Both Runxa and Runxb transactivate an AML1-responsive human promoter in hematopoietic cells. Genomic localization studies demonstrate that runxa is located on linkage group 1 (LG1), and the runxb gene is located on LG13.
Conclusions: Our gene expression analysis strongly suggests that both the functional and spatial aorta-gonad-mesonephros (AGM) region has been conserved throughout evolution. Our runxa spatiotemporal expression data shed light on the role of vertebrate Runx1/AML1 in primitive vs definitive hematopoietic development.
Similar articles
- Cloning and embryonic expression patterns of the zebrafish Runt domain genes, runxa and runxb.
Kataoka H, Ochi M, Enomoto K, Yamaguchi A. Kataoka H, et al. Mech Dev. 2000 Nov;98(1-2):139-43. doi: 10.1016/s0925-4773(00)00445-7. Mech Dev. 2000. PMID: 11044618 - Runx3 is required for hematopoietic development in zebrafish.
Kalev-Zylinska ML, Horsfield JA, Flores MV, Postlethwait JH, Chau JY, Cattin PM, Vitas MR, Crosier PS, Crosier KE. Kalev-Zylinska ML, et al. Dev Dyn. 2003 Nov;228(3):323-36. doi: 10.1002/dvdy.10388. Dev Dyn. 2003. PMID: 14579373 - Runx1 is required for zebrafish blood and vessel development and expression of a human RUNX1-CBF2T1 transgene advances a model for studies of leukemogenesis.
Kalev-Zylinska ML, Horsfield JA, Flores MV, Postlethwait JH, Vitas MR, Baas AM, Crosier PS, Crosier KE. Kalev-Zylinska ML, et al. Development. 2002 Apr;129(8):2015-30. doi: 10.1242/dev.129.8.2015. Development. 2002. PMID: 11934867 - Pathways in blood and vessel development revealed through zebrafish genetics.
Crosier PS, Kalev-Zylinska ML, Hall CJ, Flores MV, Horsfield JA, Crosier KE. Crosier PS, et al. Int J Dev Biol. 2002;46(4):493-502. Int J Dev Biol. 2002. PMID: 12141436 Review. - RUNX1/AML1: a central player in hematopoiesis.
Okuda T, Nishimura M, Nakao M, Fujita Y. Okuda T, et al. Int J Hematol. 2001 Oct;74(3):252-7. doi: 10.1007/BF02982057. Int J Hematol. 2001. PMID: 11721959 Review.
Cited by
- Exploring hematopoiesis in zebrafish using forward genetic screening.
Song H, Shin U, Nam U, Lee Y. Song H, et al. Exp Mol Med. 2024 Feb;56(1):51-58. doi: 10.1038/s12276-023-01138-2. Epub 2024 Jan 4. Exp Mol Med. 2024. PMID: 38172599 Free PMC article. Review. - Splicing factor deficits render hematopoietic stem and progenitor cells sensitive to STAT3 inhibition.
Potts KS, Cameron RC, Metidji A, Ghazale N, Wallace L, Leal-Cervantes AI, Palumbo R, Barajas JM, Gupta V, Aluri S, Pradhan K, Myers JA, McKinstry M, Bai X, Choudhary GS, Shastri A, Verma A, Obeng EA, Bowman TV. Potts KS, et al. Cell Rep. 2022 Dec 13;41(11):111825. doi: 10.1016/j.celrep.2022.111825. Cell Rep. 2022. PMID: 36516770 Free PMC article. - Dhx15 regulates zebrafish definitive hematopoiesis through the unfolded protein response pathway.
Cai Y, Wang J, Jin D, Liu Q, Chen X, Pan L, Li Y, Wang X, Qian F, Wang J, Zhong TP, Wang S. Cai Y, et al. Cancer Sci. 2021 Sep;112(9):3884-3894. doi: 10.1111/cas.15002. Epub 2021 Jul 11. Cancer Sci. 2021. PMID: 34077586 Free PMC article. - Antisense RNAs during early vertebrate development are divided in groups with distinct features.
Pillay S, Takahashi H, Carninci P, Kanhere A. Pillay S, et al. Genome Res. 2021 Jun;31(6):995-1010. doi: 10.1101/gr.262964.120. Epub 2021 Apr 1. Genome Res. 2021. PMID: 33795334 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous