Adhesion of dendritic cells derived from CD34+ progenitors to resting human dermal microvascular endothelial cells is down-regulated upon maturation and partially depends on CD11a-CD18, CD11b-CD18 and CD36 - PubMed (original) (raw)
Adhesion of dendritic cells derived from CD34+ progenitors to resting human dermal microvascular endothelial cells is down-regulated upon maturation and partially depends on CD11a-CD18, CD11b-CD18 and CD36
Van Anh Nguyen et al. Eur J Immunol. 2002 Dec.
Free article
Abstract
DC are sentinels of the immune system. In order to reach the skin, bone-marrow-derived DC precursors need to bind and migrate through microvascular endothelial cells. Binding of DC toprimary endothelial cells of the skin has not been investigated. We therefore determined adhesion of DC at different stages of development to human dermal microvascular endothelial cells (HDMEC). DC were derived from CD34+ progenitors in cord blood. To enhance DC maturation, a defined cocktail of IL-1beta+IL-6+TNF-alpha+PGE2 was applied. Adhesion was quantified by fluorimetric and phase-contrast microscopical assays. Significantly more DC precursors (tested on day 5 after isolation) than mature DC (spontaneously matured or cytokine-cocktail-matured and tested on day 13) bound to unstimulated HDMEC. In contrast, the maturation stage of DC had no influence on their binding to human umbilical vein endothelial cells. Pretreatment of HDMEC with TNF-alpha and IFN-gamma resulted in an enhanced attachment of both DC precursors and mature DC. Mature DC lacked expression of CD31, CD36, CD45RA and CLA, and expressed lower levels of CD11a, CD11b and CD49d as compared with precursors tested on day 5. mAb against CD18, CD11a, CD11b, and CD36 markedly inhibited DC binding, whereas anti-CLA, anti-DC-SIGN, anti-CD29 and anti-CD49 mAb did not. Our data support the hypothesis of immunosurveillance with selective recruitment of blood DC precursors to resting and, more so, to inflamed skin. The data have potential relevance for anti-cancer immunotherapy strategies favoring the intracutaneous application of mature DC.
Similar articles
- Adhesion of monocyte-derived dendritic cells to human umbilical vein endothelial cells in flow field decreases upon maturation.
Jiang Y, Zeng Z, Sun D, Ka W, Wen Z. Jiang Y, et al. Clin Hemorheol Microcirc. 2005;32(4):261-8. Clin Hemorheol Microcirc. 2005. PMID: 15894824 - Selective generation of different dendritic cell precursors from CD34+ cells by interleukin-6 and interleukin-3.
Encabo A, Solves P, Mateu E, Sepúlveda P, Carbonell-Uberos F, Miñana MD. Encabo A, et al. Stem Cells. 2004;22(5):725-40. doi: 10.1634/stemcells.22-5-725. Stem Cells. 2004. PMID: 15342937 - Tumor necrosis factor alpha-stimulated endothelium: an inducer of dendritic cell development from hematopoietic progenitors and myeloid leukemic cells.
Moldenhauer A, Nociari M, Lam G, Salama A, Rafii S, Moore MA. Moldenhauer A, et al. Stem Cells. 2004;22(2):144-57. doi: 10.1634/stemcells.22-2-144. Stem Cells. 2004. PMID: 14990854 - In vitro generation of human dendritic cells and cell therapy.
Gluckman JC, Canque B, Chapuis F, Rosenzwajg M. Gluckman JC, et al. Cytokines Cell Mol Ther. 1997 Sep;3(3):187-96. Cytokines Cell Mol Ther. 1997. PMID: 9426977 Review. - Dendritic cell differentiation from hematopoietic CD34+ progenitor cells.
Curti A, Fogli M, Ratta M, Biasco G, Tura S, Lemoli RM. Curti A, et al. J Biol Regul Homeost Agents. 2001 Jan-Mar;15(1):49-52. J Biol Regul Homeost Agents. 2001. PMID: 11388744 Review.
Cited by
- Personalized risk predictor for acute cellular rejection in lung transplant using soluble CD31.
Tran-Dinh A, Laurent Q, Even G, Tanaka S, Lortat-Jacob B, Castier Y, Mal H, Messika J, Mordant P, Nicoletti A, Montravers P, Caligiuri G, Morilla I. Tran-Dinh A, et al. Sci Rep. 2022 Oct 21;12(1):17628. doi: 10.1038/s41598-022-21070-1. Sci Rep. 2022. PMID: 36271122 Free PMC article. - 1,25-Dihydroxyvitamin D3 Restrains CD4+ T Cell Priming Ability of CD11c+ Dendritic Cells by Upregulating Expression of CD31.
Saul L, Mair I, Ivens A, Brown P, Samuel K, Campbell JDM, Soong DY, Kamenjarin N, Mellanby RJ. Saul L, et al. Front Immunol. 2019 Mar 28;10:600. doi: 10.3389/fimmu.2019.00600. eCollection 2019. Front Immunol. 2019. PMID: 30984180 Free PMC article. - Interleukin-10 reorganizes the cytoskeleton of mature dendritic cells leading to their impaired biophysical properties and motilities.
Xu X, Liu X, Long J, Hu Z, Zheng Q, Zhang C, Li L, Wang Y, Jia Y, Qiu W, Zhou J, Yao W, Zeng Z. Xu X, et al. PLoS One. 2017 Feb 24;12(2):e0172523. doi: 10.1371/journal.pone.0172523. eCollection 2017. PLoS One. 2017. PMID: 28234961 Free PMC article. - CD31 is a key coinhibitory receptor in the development of immunogenic dendritic cells.
Clement M, Fornasa G, Guedj K, Ben Mkaddem S, Gaston AT, Khallou-Laschet J, Morvan M, Nicoletti A, Caligiuri G. Clement M, et al. Proc Natl Acad Sci U S A. 2014 Mar 25;111(12):E1101-10. doi: 10.1073/pnas.1314505111. Epub 2014 Mar 10. Proc Natl Acad Sci U S A. 2014. PMID: 24616502 Free PMC article. - CD34+ -derived Langerhans cell-like cells are different from epidermal Langerhans cells in their response to thymic stromal lymphopoietin.
Nguyen VA, Dubrac S, Forstner M, Huter O, Del Frari B, Romani N, Ebner S. Nguyen VA, et al. J Cell Mol Med. 2011 Sep;15(9):1847-56. doi: 10.1111/j.1582-4934.2010.01206.x. J Cell Mol Med. 2011. PMID: 21054781 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials