Effect of cold preservation on intracellular calcium concentration and calpain activity in rat sinusoidal endothelial cells - PubMed (original) (raw)

Effect of cold preservation on intracellular calcium concentration and calpain activity in rat sinusoidal endothelial cells

G Aravinda Upadhya et al. Hepatology. 2003 Feb.

Abstract

This study was performed to determine the role of intracellular calcium concentration and calpain activity on the cellular events that occur in rat sinusoidal endothelial cells (SEC) in the cold. Intracellular calcium concentrations were measured in isolated cold preserved rat SEC. Dantrolene or 1,2-bis(o-Aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetra(acetoxymethyl) ester (BAPTA-AM) was added in some studies. In other studies, calpain activity and m-calpain and mu-calpain expression were measured during cold preservation in the presence or absence of calpain inhibitors. The effect of addition of dantrolene to preservation solutions on function of whole livers after preservation was determined. Cold preservation caused an increase in intracellular calcium concentration first detected at 1 hour of preservation. This was associated with cell rounding and actin disassembly. Dantrolene and BAPTA-AM prevented the increase in intracellular calcium concentration and reduced cell rounding and actin disassembly. Cold preservation also resulted in increased calpain activity and expression on SEC. Calpain expression was reduced by dantrolene. Calpain inhibitors N-acetyl-leu-leu-norleucinal (ALLN) and N-acetyl-leu-leu-methioninal (ALLM) reduced calpain activity and expression and restored SEC cell shape and actin morphology. Dantrolene improved function of livers preserved in Eurocollins (EC) solution when tested on the isolated perfused rat liver (IPRL). In conclusion, exposure of SEC to cold results sequentially in elevated intracellular calcium concentration, increased calpain activity, and actin disassembly.

PubMed Disclaimer

Similar articles

• Leukocyte adhesion to cold-preserved rat endothelial cells: role of actin disassembly and ICAM-1.
  Topp SA, Upadhya GA, Strasberg SM. Topp SA, et al. Liver Transpl. 2003 Dec;9(12):1286-94. doi: 10.1016/j.lts.2003.09.008. Liver Transpl. 2003. PMID: 14625829
• Cold preservation-warm reperfusion perturbs cytosolic calcium ion homeostasis in rat liver sinusoidal endothelial cells.
  Auger S, Vallerand D, Haddad PS. Auger S, et al. Liver Transpl. 2003 Feb;9(2):150-9. doi: 10.1053/jlts.2003.50025. Liver Transpl. 2003. PMID: 12548509
• Chelation of intracellular Ca2+ inhibits murine keratinocyte differentiation in vitro.
  Li L, Tucker RW, Hennings H, Yuspa SH. Li L, et al. J Cell Physiol. 1995 Apr;163(1):105-14. doi: 10.1002/jcp.1041630112. J Cell Physiol. 1995. PMID: 7896886
• Na+/H+ exchanger is required for hyperglycaemia-induced endothelial dysfunction via calcium-dependent calpain.
  Wang S, Peng Q, Zhang J, Liu L. Wang S, et al. Cardiovasc Res. 2008 Nov 1;80(2):255-62. doi: 10.1093/cvr/cvn179. Epub 2008 Jun 30. Cardiovasc Res. 2008. PMID: 18591204
• Hypothermic preservation up-regulates calpain expression and increases ubiquitination in cultured vascular endothelial cells: influence of dopamine pretreatment.
  Rudic B, Song H, Breedijk A, Brinkkoetter P, Beck G, Yard B, Ponelies N. Rudic B, et al. J Surg Res. 2010 May 15;160(2):325-32. doi: 10.1016/j.jss.2008.12.025. Epub 2009 Jan 19. J Surg Res. 2010. PMID: 19375722

Cited by

• Arginine promotes myogenic differentiation and myotube formation through the elevation of cytoplasmic calcium concentration.
  Gong L, Zhang X, Qiu K, He L, Wang Y, Yin J. Gong L, et al. Anim Nutr. 2021 Dec;7(4):1115-1123. doi: 10.1016/j.aninu.2021.05.010. Epub 2021 Sep 28. Anim Nutr. 2021. PMID: 34738042 Free PMC article.
• Angiocrine Signaling in Sinusoidal Health and Disease.
  Cooper SA, Kostallari E, Shah VH. Cooper SA, et al. Semin Liver Dis. 2023 Aug;43(3):245-257. doi: 10.1055/a-2128-5907. Epub 2023 Jul 13. Semin Liver Dis. 2023. PMID: 37442155 Free PMC article. Review.
• The synergy between diurnal temperature range and calcium concentration help to predict hospital mortality in patients with acute myocardial infarction.
  Gu X, Liu D, Hao N, Sun X, Liu S, Duan X, Yang S, Li J, Wang S. Gu X, et al. Sci Rep. 2022 Sep 15;12(1):15527. doi: 10.1038/s41598-022-18816-2. Sci Rep. 2022. PMID: 36109534 Free PMC article.
• Liver graft exposure to carbon monoxide during cold storage protects sinusoidal endothelial cells and ameliorates reperfusion injury in rats.
  Ikeda A, Ueki S, Nakao A, Tomiyama K, Ross MA, Stolz DB, Geller DA, Murase N. Ikeda A, et al. Liver Transpl. 2009 Nov;15(11):1458-68. doi: 10.1002/lt.21918. Liver Transpl. 2009. PMID: 19877256 Free PMC article.
• Ischaemia reperfusion injury in liver transplantation: Cellular and molecular mechanisms.
  Dar WA, Sullivan E, Bynon JS, Eltzschig H, Ju C. Dar WA, et al. Liver Int. 2019 May;39(5):788-801. doi: 10.1111/liv.14091. Epub 2019 Apr 2. Liver Int. 2019. PMID: 30843314 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science
  • Ovid Technologies, Inc.
  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal