MAPK signal transduction pathway mediates agrin effects on neurite elongation in cultured hippocampal neurons - PubMed (original) (raw)
Comparative Study
doi: 10.1002/neu.10197.
Affiliations
- PMID: 12605455
- DOI: 10.1002/neu.10197
Comparative Study
MAPK signal transduction pathway mediates agrin effects on neurite elongation in cultured hippocampal neurons
Lisa Karasewski et al. J Neurobiol. 2003 Apr.
Abstract
We have previously shown that agrin regulates the rates of axonal and dendritic elongation by modulating the expression of microtubule-associated proteins in cultured hippocampal neurons. However, the mechanisms by which agrin-induced signals are propagated to the nucleus where they can lead to the phosphorylation, and hence the activation, of transcription factors, are not known. In the present study, we identified downstream elements that play essential roles in the agrin-signaling pathway in developing central neurons. Our results indicate that agrin induces the combined activation of the extracellular signal-regulated kinases (ERK1/ERK2) and p38 in central neurons. In addition, they showed that PD98059 and SB202190, synthetic inhibitors of ERK1/ERK2 and p38 respectively, prevented the changes in the rate of neurite elongation induced by agrin in cultured hippocampal neurons. Collectively, these results suggest that agrin might modulate the expression of neuron-specific genes involved in neurite elongation by inducing CREB phosphorylation through the activation of the MAPK signal transduction pathway in cultured hippocampal neurons.
Copyright 2003 Wiley Periodicals, Inc. J Neurobiol 55: 14-24, 2003
Similar articles
- Agrin differentially regulates the rates of axonal and dendritic elongation in cultured hippocampal neurons.
Mantych KB, Ferreira A. Mantych KB, et al. J Neurosci. 2001 Sep 1;21(17):6802-9. doi: 10.1523/JNEUROSCI.21-17-06802.2001. J Neurosci. 2001. PMID: 11517268 Free PMC article. - Cyclic AMP-induced neuronal differentiation via activation of p38 mitogen-activated protein kinase.
Hansen TO, Rehfeld JF, Nielsen FC. Hansen TO, et al. J Neurochem. 2000 Nov;75(5):1870-7. doi: 10.1046/j.1471-4159.2000.0751870.x. J Neurochem. 2000. PMID: 11032876 - Specific agrin isoforms induce cAMP response element binding protein phosphorylation in hippocampal neurons.
Ji RR, Böse CM, Lesuisse C, Qiu D, Huang JC, Zhang Q, Rupp F. Ji RR, et al. J Neurosci. 1998 Dec 1;18(23):9695-702. doi: 10.1523/JNEUROSCI.18-23-09695.1998. J Neurosci. 1998. PMID: 9822730 Free PMC article. - PD98059 prevents neurite degeneration induced by fibrillar beta-amyloid in mature hippocampal neurons.
Rapoport M, Ferreira A. Rapoport M, et al. J Neurochem. 2000 Jan;74(1):125-33. doi: 10.1046/j.1471-4159.2000.0740125.x. J Neurochem. 2000. PMID: 10617113
Cited by
- Emerging roles for MAP kinases in agrin signaling.
Rimer M. Rimer M. Commun Integr Biol. 2011 Mar;4(2):143-6. doi: 10.4161/cib.4.2.14357. Commun Integr Biol. 2011. PMID: 21655426 Free PMC article. - Agrin induced morphological and structural changes in growth cones of cultured hippocampal neurons.
Bergstrom RA, Sinjoanu RC, Ferreira A. Bergstrom RA, et al. Neuroscience. 2007 Nov 9;149(3):527-36. doi: 10.1016/j.neuroscience.2007.08.017. Epub 2007 Aug 14. Neuroscience. 2007. PMID: 17870250 Free PMC article. - Leucine-rich repeat kinase 2 and alpha-synuclein: intersecting pathways in the pathogenesis of Parkinson's disease?
Greggio E, Bisaglia M, Civiero L, Bubacco L. Greggio E, et al. Mol Neurodegener. 2011 Jan 18;6(1):6. doi: 10.1186/1750-1326-6-6. Mol Neurodegener. 2011. PMID: 21244648 Free PMC article. - Transmembrane form agrin-induced process formation requires lipid rafts and the activation of Fyn and MAPK.
Ramseger R, White R, Kröger S. Ramseger R, et al. J Biol Chem. 2009 Mar 20;284(12):7697-705. doi: 10.1074/jbc.M806719200. Epub 2009 Jan 12. J Biol Chem. 2009. PMID: 19139104 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous