C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression - PubMed (original) (raw)

Philippe Vaglio, Jean-François Rual, Philippe Lamesch, Monica Martinez, Christopher M Armstrong, Siming Li, Laurent Jacotot, Nicolas Bertin, Rekin's Janky, Troy Moore, James R Hudson Jr, James L Hartley, Michael A Brasch, Jean Vandenhaute, Simon Boulton, Gregory A Endress, Sarah Jenna, Eric Chevet, Vasilis Papasotiropoulos, Peter P Tolias, Jason Ptacek, Mike Snyder, Raymond Huang, Mark R Chance, Hongmei Lee, Lynn Doucette-Stamm, David E Hill, Marc Vidal

Affiliations

PMID: 12679813
DOI: 10.1038/ng1140

C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression

Jérôme Reboul et al. Nat Genet. 2003 May.

Abstract

To verify the genome annotation and to create a resource to functionally characterize the proteome, we attempted to Gateway-clone all predicted protein-encoding open reading frames (ORFs), or the 'ORFeome,' of Caenorhabditis elegans. We successfully cloned approximately 12,000 ORFs (ORFeome 1.1), of which roughly 4,000 correspond to genes that are untouched by any cDNA or expressed-sequence tag (EST). More than 50% of predicted genes needed corrections in their intron-exon structures. Notably, approximately 11,000 C. elegans proteins can now be expressed under many conditions and characterized using various high-throughput strategies, including large-scale interactome mapping. We suggest that similar ORFeome projects will be valuable for other organisms, including humans.

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ORFeomics: correcting the wiggle in worm genes.
Boone C, Andrews B. Boone C, et al. Nat Genet. 2003 May;34(1):8-9. doi: 10.1038/ng0503-8. Nat Genet. 2003. PMID: 12721544 No abstract available.

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C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression - PubMed (original) (raw)