Transcription of antisense RNA leading to gene silencing and methylation as a novel cause of human genetic disease - PubMed (original) (raw)
Transcription of antisense RNA leading to gene silencing and methylation as a novel cause of human genetic disease
Cristina Tufarelli et al. Nat Genet. 2003 Jun.
Abstract
Nearly all human genetic disorders result from a limited repertoire of mutations in an associated gene or its regulatory elements. We recently described an individual with an inherited form of anemia (alpha-thalassemia) who has a deletion that results in a truncated, widely expressed gene (LUC7L) becoming juxtaposed to a structurally normal alpha-globin gene (HBA2). Although it retains all of its local and remote cis-regulatory elements, expression of HBA2 is silenced and its CpG island becomes completely methylated early during development. Here we show that in the affected individual, in a transgenic model and in differentiating embryonic stem cells, transcription of antisense RNA mediates silencing and methylation of the associated CpG island. These findings identify a new mechanism underlying human genetic disease.
Comment in
- Turned off by RNA.
Kleinjan DA, van Heyningen V. Kleinjan DA, et al. Nat Genet. 2003 Jun;34(2):125-6. doi: 10.1038/ng0603-125. Nat Genet. 2003. PMID: 12776110 No abstract available.
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