The influence of insulin on secretion of IGF-I and IGFBP-I in cultures of human endometrial stromal cells - PubMed (original) (raw)
Affiliations
- PMID: 12775252
The influence of insulin on secretion of IGF-I and IGFBP-I in cultures of human endometrial stromal cells
Jinfang Lin et al. Chin Med J (Engl). 2003 Feb.
Abstract
Objectives: To study the influence of insulin on IGF-I and IGFBP-I secretion of the human endometrial stromal cells.
Methods: Late proliferative phase endometrial stromal cells were isolated from endometrium tissues and then cultured for 24 h in Hams F-12 only as a control and in Hams F-12 with different concentrations of estradiol (E2) and insulin (INS) as treated groups. Simultaneously, the endometrial stromal cells from late secretory phase endometrium were cultured for 24 h in Hams F-12 only as a control and in Hams F-12 supplemented with different concentrations of progesterone (P) and insulin as treated groups. After 24 h of culturing, the mediums were collected for either IGF-I or IGFBP-I assays.
Result: The concentrations of IGF-I in medium from cultured endometrial stromal cells in the proliferative phase were 0.78 +/- 0.47 ng/ml in the hormone-free control group; 1.44 +/- 0.59 ng/ml and 1.39 +/- 0.33 ng/ml in 100 pg/ml E2 group and 20 microU/ml INS group, which was higher than that of the control group (P < 0.05 and P < 0.01, respectively). The IGF-I concentration in the 100 microU/ml INS group was 2.03 +/- 0.53 ng/ml, which was higher than that of the 20 micro U/ml INS group (P < 0.01). Levels of IGF-I in the 100 pg/ml E2 plus 20 microU/ml INS group was 2.18 +/- 0.36 ng/ml, which was significantly higher than that of the 20 microU/ml INS and 100 pg/ml E2 group (P < 0.01), but lower than that of the 100 pg/ml E2 plus 100 microU/ml INS group (3.42 +/- 0.75 ng/ml), P < 0.01. The concentration of IGFBP-I in medium from cultured endometrial stromal cells in the secretory phase was 2.50 +/- 1.39 ng/ml in the hormone-free control group and 5.44 +/- 2.09 ng/ml in the 10 pg/ml P group, which was significantly higher than that of the control (P < 0.01). IGFBP-I concentration in 20 microU/ml INS group was 0.16 +/- 0.58 ng/ml, which was lower compared with control, but higher compared with the 100 microU/ml INS group (P < 0.01). The level of IGFBP-I in the 10 ng/ml P plus 20 microU/ml INS group was 2.10 +/- 1.17 ng/ml, lower compared with the 10 ng/ml P group, but higher compared with the 10 pg/ml P plus 100 microU/ml INS group, P < 0.01.
Conclusions: Insulin can stimulate basal (without hormone) and E2-stimulated IGF-I secretion in cultured stromal cells from human late proliferative endometrium in a dose-dependent manner. Insulin can suppress basal (without hormone) and P-stimulated IGFBP-I secretions in cultured stromal cells from human secretory endometrium in a dose-dependent manner.
Similar articles
- Insulin-like growth factor binding protein-1 induces decidualization of human endometrial stromal cells via alpha5beta1 integrin.
Matsumoto H, Sakai K, Iwashita M. Matsumoto H, et al. Mol Hum Reprod. 2008 Aug;14(8):485-9. doi: 10.1093/molehr/gan038. Epub 2008 Jun 26. Mol Hum Reprod. 2008. PMID: 18583428 - Effect of leptin on prolactin and insulin-like growth factor-I secretion by cultured rat endometrial stromal cells.
Tennekoon KH, Eswaramohan T, Karunanayake EH. Tennekoon KH, et al. Fertil Steril. 2007 Jul;88(1):193-9. doi: 10.1016/j.fertnstert.2006.11.115. Epub 2007 Feb 20. Fertil Steril. 2007. PMID: 17307172 - Multifaceted roles for IGFBP-1 in human endometrium during implantation and pregnancy.
Giudice LC. Giudice LC. Ann N Y Acad Sci. 1997 Sep 26;828:146-56. doi: 10.1111/j.1749-6632.1997.tb48533.x. Ann N Y Acad Sci. 1997. PMID: 9329833 Review. - Biochemical characteristics of human endometrial cells.
Gurpide E. Gurpide E. Contrib Gynecol Obstet. 1991;18:15-23. Contrib Gynecol Obstet. 1991. PMID: 1935125 Review. No abstract available.
Cited by
- A Prospective Cohort Study of Metformin as an Adjuvant Therapy for Infertile Women With Endometrial Complex Hyperplasia/Complex Atypical Hyperplasia and Their Subsequent Assisted Reproductive Technology Outcomes.
Kong WY, Liu ZA, Zhang N, Wu X, Zhao XB, Yan L. Kong WY, et al. Front Endocrinol (Lausanne). 2022 Jun 30;13:849794. doi: 10.3389/fendo.2022.849794. eCollection 2022. Front Endocrinol (Lausanne). 2022. PMID: 35846327 Free PMC article. Clinical Trial. - Conservative treatment for atypical endometrial hyperplasia: what is the most effective therapeutic method?
Kim MK, Seong SJ. Kim MK, et al. J Gynecol Oncol. 2014 Jul;25(3):164-5. doi: 10.3802/jgo.2014.25.3.164. J Gynecol Oncol. 2014. PMID: 25045426 Free PMC article. No abstract available. - Direct effects of metformin in the endometrium: a hypothetical mechanism for the treatment of women with PCOS and endometrial carcinoma.
Shao R, Li X, Feng Y, Lin JF, Billig H. Shao R, et al. J Exp Clin Cancer Res. 2014 May 11;33(1):41. doi: 10.1186/1756-9966-33-41. J Exp Clin Cancer Res. 2014. PMID: 24887156 Free PMC article. Review. - Combination of Diane-35 and Metformin to Treat Early Endometrial Carcinoma in PCOS Women with Insulin Resistance.
Li X, Guo YR, Lin JF, Feng Y, Billig H, Shao R. Li X, et al. J Cancer. 2014 Jan 28;5(3):173-81. doi: 10.7150/jca.8009. eCollection 2014. J Cancer. 2014. PMID: 24563672 Free PMC article. - Endometrial progesterone resistance and PCOS.
Li X, Feng Y, Lin JF, Billig H, Shao R. Li X, et al. J Biomed Sci. 2014 Jan 9;21(1):2. doi: 10.1186/1423-0127-21-2. J Biomed Sci. 2014. PMID: 24405633 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Medical
Miscellaneous