Development of genetic circuitry exhibiting toggle switch or oscillatory behavior in Escherichia coli - PubMed (original) (raw)
Development of genetic circuitry exhibiting toggle switch or oscillatory behavior in Escherichia coli
Mariette R Atkinson et al. Cell. 2003.
Free article
Abstract
Analysis of the system design principles of signaling systems requires model systems where all components and regulatory interactions are known. Components of the Lac and Ntr systems were used to construct genetic circuits that display toggle switch or oscillatory behavior. Both devices contain an "activator module" consisting of a modified glnA promoter with lac operators, driving the expression of the activator, NRI. Since NRI activates the glnA promoter, this creates an autoactivated circuit repressible by LacI. The oscillator contains a "repressor module" consisting of the NRI-activated glnK promoter driving LacI expression. This circuitry produced synchronous damped oscillations in turbidostat cultures, with periods much longer than the cell cycle. For the toggle switch, LacI was provided constitutively; the level of active repressor was controlled by using a lacY mutant and varying the concentration of IPTG. This circuitry provided nearly discontinuous expression of activator.
Similar articles
- Role of multiple environmental stimuli in control of transcription from a nitrogen-regulated promoter in Escherichia coli with weak or no activator-binding sites.
Schneider BL, Shiau SP, Reitzer LJ. Schneider BL, et al. J Bacteriol. 1991 Oct;173(20):6355-63. doi: 10.1128/jb.173.20.6355-6363.1991. J Bacteriol. 1991. PMID: 1680849 Free PMC article. - Construction of a genetic toggle switch in Escherichia coli.
Gardner TS, Cantor CR, Collins JJ. Gardner TS, et al. Nature. 2000 Jan 20;403(6767):339-42. doi: 10.1038/35002131. Nature. 2000. PMID: 10659857 - Activation of the glnA, glnK, and nac promoters as Escherichia coli undergoes the transition from nitrogen excess growth to nitrogen starvation.
Atkinson MR, Blauwkamp TA, Bondarenko V, Studitsky V, Ninfa AJ. Atkinson MR, et al. J Bacteriol. 2002 Oct;184(19):5358-63. doi: 10.1128/JB.184.19.5358-5363.2002. J Bacteriol. 2002. PMID: 12218022 Free PMC article. - Activation of glnA transcription by nitrogen regulator I (NRI)-phosphate in Escherichia coli: evidence for a long-range physical interaction between NRI-phosphate and RNA polymerase.
Reitzer LJ, Movsas B, Magasanik B. Reitzer LJ, et al. J Bacteriol. 1989 Oct;171(10):5512-22. doi: 10.1128/jb.171.10.5512-5522.1989. J Bacteriol. 1989. PMID: 2571609 Free PMC article. - Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production.
Browning DF, Godfrey RE, Richards KL, Robinson C, Busby SJW. Browning DF, et al. Biochem Soc Trans. 2019 Apr 30;47(2):755-763. doi: 10.1042/BST20190059. Epub 2019 Apr 10. Biochem Soc Trans. 2019. PMID: 30971435 Review.
Cited by
- Phenotypic deconstruction of gene circuitry.
Lomnitz JG, Savageau MA. Lomnitz JG, et al. Chaos. 2013 Jun;23(2):025108. doi: 10.1063/1.4809776. Chaos. 2013. PMID: 23822506 Free PMC article. - Control theory meets synthetic biology.
Del Vecchio D, Dy AJ, Qian Y. Del Vecchio D, et al. J R Soc Interface. 2016 Jul;13(120):20160380. doi: 10.1098/rsif.2016.0380. Epub 2016 Jul 20. J R Soc Interface. 2016. PMID: 27440256 Free PMC article. Review. - Molecular recordings by directed CRISPR spacer acquisition.
Shipman SL, Nivala J, Macklis JD, Church GM. Shipman SL, et al. Science. 2016 Jul 29;353(6298):aaf1175. doi: 10.1126/science.aaf1175. Epub 2016 Jun 9. Science. 2016. PMID: 27284167 Free PMC article. - Biology by design: reduction and synthesis of cellular components and behaviour.
Marguet P, Balagadde F, Tan C, You L. Marguet P, et al. J R Soc Interface. 2007 Aug 22;4(15):607-23. doi: 10.1098/rsif.2006.0206. J R Soc Interface. 2007. PMID: 17251159 Free PMC article. Review. - How to turn a genetic circuit into a synthetic tunable oscillator, or a bistable switch.
Marucci L, Barton DA, Cantone I, Ricci MA, Cosma MP, Santini S, di Bernardo D, di Bernardo M. Marucci L, et al. PLoS One. 2009 Dec 7;4(12):e8083. doi: 10.1371/journal.pone.0008083. PLoS One. 2009. PMID: 19997611 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous