Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene - PubMed (original) (raw)
. 2003 Jun;21(10):988-92.
doi: 10.1007/s00299-003-0609-6. Epub 2003 Apr 3.
Affiliations
- PMID: 12835909
- DOI: 10.1007/s00299-003-0609-6
Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene
T Anai et al. Plant Cell Rep. 2003 Jun.
Abstract
Microsomal omega-3 fatty acid desaturase is an essential enzyme in the production of the n-3 polyunsaturated fatty acid alpha-linolenic acid during the seed developing stage. We have constructed a chimeric gene consisting of a maize Ubi1-P-int and a soybean GmFAD3 cDNA, which was introduced into rice plants by Agrobacterium-mediated transformation. Ten transformants containing the chimeric gene were established and expression subsequently confirmed by Northern blotting. Furthermore, alpha-linolenic acid content of the T(1) seeds increased dramatically up to tenfold that of the control, and this phenotype was also stably inherited in the T(2) and T(3) progenies. These results demonstrate that the alpha-linolenic acid content of rice seed oil can easily be altered using the combination of a high-activity promoter and a GmFAD3 gene.
Similar articles
- Identification and evaluation of ω-3 fatty acid desaturase genes for hyperfortifying α-linolenic acid in transgenic rice seed.
Liu HL, Yin ZJ, Xiao L, Xu YN, Qu le Q. Liu HL, et al. J Exp Bot. 2012 May;63(8):3279-87. doi: 10.1093/jxb/ers051. Epub 2012 Feb 29. J Exp Bot. 2012. PMID: 22378946 Free PMC article. - Developmental and growth temperature regulation of two different microsomal omega-6 desaturase genes in soybeans.
Heppard EP, Kinney AJ, Stecca KL, Miao GH. Heppard EP, et al. Plant Physiol. 1996 Jan;110(1):311-9. doi: 10.1104/pp.110.1.311. Plant Physiol. 1996. PMID: 8587990 Free PMC article. - Ribozyme termination of RNA transcripts down-regulate seed fatty acid genes in transgenic soybean.
Buhr T, Sato S, Ebrahim F, Xing A, Zhou Y, Mathiesen M, Schweiger B, Kinney A, Staswick P; Tom Clemente. Buhr T, et al. Plant J. 2002 Apr;30(2):155-63. doi: 10.1046/j.1365-313x.2002.01283.x. Plant J. 2002. PMID: 12000452 - Unsaturated Lipids Change in Olive Tree Drupe and Seed during Fruit Development and in Response to Cold-Stress and Acclimation.
D'Angeli S, Altamura MM. D'Angeli S, et al. Int J Mol Sci. 2016 Nov 12;17(11):1889. doi: 10.3390/ijms17111889. Int J Mol Sci. 2016. PMID: 27845749 Free PMC article. Review. - Biological Roles of Lipids in Rice.
Zhou K, Luo Z, Huang W, Liu Z, Miao X, Tao S, Wang J, Zhang J, Wang S, Zeng X. Zhou K, et al. Int J Mol Sci. 2024 Aug 21;25(16):9046. doi: 10.3390/ijms25169046. Int J Mol Sci. 2024. PMID: 39201734 Free PMC article. Review.
Cited by
- Improving linolenic acid content in rapeseed oil by overexpression of CsFAD2 and CsFAD3 genes.
Liu Y, Du Z, Li Y, Lu S, Tang S, Guo L. Liu Y, et al. Mol Breed. 2024 Jan 29;44(2):9. doi: 10.1007/s11032-024-01445-0. eCollection 2024 Feb. Mol Breed. 2024. PMID: 38298744 - Exogenous Jasmonic Acid Alleviates Blast Resistance Reduction Caused by LOX3 Knockout in Rice.
Su S, Tang P, Zuo R, Chen H, Zhao T, Yang S, Yang J. Su S, et al. Biomolecules. 2023 Jul 31;13(8):1197. doi: 10.3390/biom13081197. Biomolecules. 2023. PMID: 37627262 Free PMC article. - Progress in understanding and improving oil content and quality in seeds.
Sagun JV, Yadav UP, Alonso AP. Sagun JV, et al. Front Plant Sci. 2023 Jan 26;14:1116894. doi: 10.3389/fpls.2023.1116894. eCollection 2023. Front Plant Sci. 2023. PMID: 36778708 Free PMC article. Review. - Genome Editing and Protein Energy Malnutrition.
Moreno-Nombela S, Romero-Parra J, Ruiz-Ojeda FJ, Solis-Urra P, Baig AT, Plaza-Diaz J. Moreno-Nombela S, et al. Adv Exp Med Biol. 2023;1396:215-232. doi: 10.1007/978-981-19-5642-3_15. Adv Exp Med Biol. 2023. PMID: 36454470 - Heterologous Expression of Jatropha curcas Fatty Acyl-ACP Thioesterase A (JcFATA) and B (JcFATB) Affects Fatty Acid Accumulation and Promotes Plant Growth and Development in Arabidopsis.
Liu Y, Han J, Li Z, Jiang Z, Luo L, Zhang Y, Chen M, Yang Y, Liu Z. Liu Y, et al. Int J Mol Sci. 2022 Apr 11;23(8):4209. doi: 10.3390/ijms23084209. Int J Mol Sci. 2022. PMID: 35457027 Free PMC article.
References
- Plant Physiol. 1992 Nov;100(3):1503-7 - PubMed
- Am J Clin Nutr. 1991 Sep;54(3):438-63 - PubMed
- Science. 1992 Nov 20;258(5086):1353-5 - PubMed
- Ann Nutr Metab. 2000;44(5-6):263-5 - PubMed
- Nucleic Acids Res. 1980 Oct 10;8(19):4321-5 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources