Micronuclei to detect in vivo chemotherapy damage in a p53 mutated solid tumour - PubMed (original) (raw)

Micronuclei to detect in vivo chemotherapy damage in a p53 mutated solid tumour

G Driessens et al. Br J Cancer. 2003.

Abstract

Apoptosis induction and micronuclei formation were compared following cytotoxic treatments in two rat glioma differing in p53 integrity. In vitro, micronuclei emergence but not apoptosis was linked to the p53 mutated status. In vivo, micronuclei assays were more sensitive to evaluate DNA damage induced by chemotherapy in a p53-mutated solid tumour.

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Figures

Figure 1

In vitro induction of apoptosis or MN formation after chemo- or radiotherapy. Measure of caspase-3 activity in 9L (A) and C6 (C) cells. Measure of PS externalisation by AnnexinV–FITC binding assay on 9L (B) and C6 cells (D). Total percentages of 9L (E) and C6 (F) binucleated cells with MN. Significantly different from the control: *P<0.05, **P<0.01.

Figure 2

In vivo induction of apoptosis or MN formation in 9L tumours after local _γ_-irradiation or systemic injection of cisplatin. TUNEL assay on tumour section from control nontreated rat (A), cisplatin (1 mg/kg−1) treated rat (B) or locally _γ_-irradiated rat (C). Total percentages of binucleated (BN) cells with MN (D) in 9L tumours. Percentages of BN cells with MN divided into subcategories depending on the number of MN per cell (E). *Significantly different from the control (P<0.05). Magnification bars=0.1 mm.

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Micronuclei to detect in vivo chemotherapy damage in a p53 mutated solid tumour - PubMed (original) (raw)